FastQCFastQC Report
Fri 27 May 2016
SRR2139180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447491
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82450.054.6122251
GTATCAA160650.036.7376251
TGCGCGA1651.900241E-532.79432394
TCAACGC213400.030.5357994
TATCAAC215750.030.2438342
ATCAACG215300.030.2279873
CAACGCA216600.030.0271725
ACCGAAC10050.029.1547268
AACGCAG223750.029.1045366
GGACCGA10200.027.91686
CCGAACC10450.027.2489349
GACCGAA11000.025.511327
ACGCAGA255100.025.479267
AGGACCG12200.025.3698655
CGCAGAG258150.025.1782268
GCAGAGT289350.022.50619
TGGTATC34900.020.9316582
GAGTACT152000.020.47125412-13
ACATGGG247200.020.3004993
GTGGTAT36200.020.2939781
Produced by FastQC (version 0.11.5)