##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139180_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4447491 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.13653046178171 18.0 18.0 30.0 18.0 32.0 2 28.354311003664762 28.0 27.0 32.0 25.0 33.0 3 30.414119106705332 31.0 29.0 33.0 27.0 33.0 4 31.409526629733485 33.0 31.0 33.0 29.0 33.0 5 32.626266584912706 33.0 33.0 33.0 32.0 33.0 6 36.61922328791672 38.0 37.0 38.0 34.0 38.0 7 37.052534788715704 38.0 38.0 38.0 35.0 38.0 8 37.233343024190496 38.0 38.0 38.0 36.0 38.0 9 37.39806466162607 38.0 38.0 38.0 37.0 38.0 10-11 37.459651183105265 38.0 38.0 38.0 37.0 38.0 12-13 37.496537485966805 38.0 38.0 38.0 37.0 38.0 14-15 37.50234772819102 38.0 38.0 38.0 37.0 38.0 16-17 37.526293139210395 38.0 38.0 38.0 37.0 38.0 18-19 37.5278505341551 38.0 38.0 38.0 37.0 38.0 20-21 37.52997052160418 38.0 38.0 38.0 37.0 38.0 22-23 37.538023376486876 38.0 38.0 38.0 37.5 38.0 24-25 37.54369387043308 38.0 38.0 38.0 38.0 38.0 26-27 37.5127905674463 38.0 38.0 38.0 37.5 38.0 28-29 37.49207660503319 38.0 38.0 38.0 37.0 38.0 30-31 37.48640717128172 38.0 38.0 38.0 37.0 38.0 32-33 37.473893458217205 38.0 38.0 38.0 37.0 38.0 34-35 37.44718417748811 38.0 38.0 38.0 37.0 38.0 36-37 37.42267378817991 38.0 38.0 38.0 37.0 38.0 38-39 37.404141471030826 38.0 38.0 38.0 37.0 38.0 40-41 37.388306337529464 38.0 38.0 38.0 37.0 38.0 42-43 37.33860199088465 38.0 38.0 38.0 37.0 38.0 44-45 37.28185646988094 38.0 38.0 38.0 37.0 38.0 46-47 37.22675494875661 38.0 38.0 38.0 37.0 38.0 48-49 37.16749451276728 38.0 38.0 38.0 37.0 38.0 50-51 37.124814912607945 38.0 38.0 38.0 37.0 38.0 52-53 37.08858332307254 38.0 38.0 38.0 36.0 38.0 54-55 37.0662563874086 38.0 38.0 38.0 36.0 38.0 56-57 37.015476699483905 38.0 38.0 38.0 36.0 38.0 58-59 36.98594902762083 38.0 38.0 38.0 36.0 38.0 60-61 37.00960979469656 38.0 38.0 38.0 36.0 38.0 62-63 37.022985548935985 38.0 38.0 38.0 36.0 38.0 64-65 37.02817147952939 38.0 38.0 38.0 36.0 38.0 66-67 37.02258686832993 38.0 38.0 38.0 36.0 38.0 68-69 36.98654593501677 38.0 38.0 38.0 36.0 38.0 70-71 36.986608800164234 38.0 38.0 38.0 36.0 38.0 72-73 36.9971386737532 38.0 38.0 38.0 36.0 38.0 74-75 37.00827787900951 38.0 38.0 38.0 36.0 38.0 76-77 36.99255258100558 38.0 38.0 38.0 36.0 38.0 78-79 36.952140779795 38.0 38.0 38.0 36.0 38.0 80-81 36.90332059789324 38.0 38.0 38.0 35.0 38.0 82-83 36.859247204341905 38.0 38.0 38.0 35.0 38.0 84-85 36.83198319804091 38.0 38.0 38.0 35.0 38.0 86-87 36.85854897354025 38.0 38.0 38.0 35.0 38.0 88-89 36.811515841114144 38.0 38.0 38.0 35.0 38.0 90-91 36.82231330153733 38.0 38.0 38.0 35.0 38.0 92-93 36.80744231202388 38.0 38.0 38.0 35.0 38.0 94-95 36.81812061486663 38.0 38.0 38.0 35.0 38.0 96-97 36.808597825719275 38.0 38.0 38.0 35.0 38.0 98-99 36.79520486109737 38.0 38.0 38.0 35.0 38.0 100 35.21719684000612 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 6.0 20 22.0 21 69.0 22 195.0 23 607.0 24 1481.0 25 3198.0 26 6162.0 27 11043.0 28 18143.0 29 27124.0 30 35229.0 31 44047.0 32 56161.0 33 77042.0 34 120625.0 35 241368.0 36 931633.0 37 2873335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.65102413922816 29.140857170930758 13.719623041395698 20.488495648445383 2 14.102423711080345 22.3662556205817 41.901269340231345 21.63005132810661 3 16.794619159956508 29.529842041028143 31.561552554208177 22.113986244807176 4 11.987536259452302 19.469355661578593 40.66868714531729 27.874420933651805 5 12.146084162958395 39.11825791215766 35.825030337329515 12.910627587554421 6 26.941797071652307 40.44028419619062 19.817600530276508 12.800318201880568 7 24.827121628801496 34.30412787794287 24.889898596759387 15.978851896496248 8 23.715483628859506 37.15937817524532 22.121258930034934 17.003879265860235 9 26.46238069958995 16.795941801793415 22.034243576884137 34.7074339217325 10-11 23.203880569966305 27.76238333028667 29.71552949741776 19.31820660232927 12-13 24.767200203440545 25.983425261568826 29.600655740506276 19.648718794484353 14-15 22.17698697984999 26.693454804068185 27.75921300346645 23.37034521261538 16-17 20.612554359300557 29.796878734549438 28.39369433237751 21.19687257377249 18-19 20.624347525380042 28.64578028375999 30.761208960287945 19.968663230572023 20-21 21.992022753074323 27.63655790721723 30.56764189585868 19.803777443849764 22-23 21.745804542576778 27.58343833618369 30.32989015429488 20.34086696694465 24-25 21.17232281028764 27.939870409356832 30.53674347453245 20.35106330582308 26-27 20.850523297026598 28.083603344835677 30.583445308526247 20.482428049611478 28-29 20.811549630936273 27.750076003074565 30.76324195293178 20.67513241305738 30-31 21.211283588005397 27.86146600337358 30.519850261913138 20.407400146707882 32-33 20.816404146982684 28.152028144600365 30.4650398216972 20.566527886719747 34-35 21.075620152514322 28.081688636677736 30.591800318481383 20.25089089232656 36-37 21.14431568930862 27.881584459568504 30.29303347893325 20.681066372189623 38-39 20.784143613994736 28.320114198060654 30.379537705261438 20.516204482683175 40-41 20.986484897606335 27.92013483415763 30.624571345466567 20.468808922769465 42-43 21.059591399701155 28.064827326865593 29.921715889302224 20.953865384131024 44-45 20.909601923342674 28.26584530912869 29.943192805203534 20.881359962325106 46-47 21.211238366832312 28.26817767909489 29.512791087067285 21.007792867005513 48-49 21.168860986459926 28.462036526374355 29.285629250254498 21.08347323691122 50-51 21.161181244447473 28.54657271859476 28.96756543141606 21.324680605541708 52-53 21.241885989840213 28.530335957392882 29.05286366075086 21.17491439201604 54-55 21.364621009375398 28.606448312699552 28.948051406410325 21.08087927151473 56-57 21.25148851254415 28.657498851864045 28.77904377547744 21.311968860114366 58-59 21.10317860775893 28.56705993397961 28.99230824283508 21.33745321542638 60-61 21.426467970726332 28.920704728400505 28.69875602896059 20.954071271912575 62-63 21.19279739555136 28.873919442984423 28.984090660734058 20.949192500730156 64-65 21.217242383426164 28.62897017193718 28.964534216576226 21.189253228060426 66-67 21.29537910733012 29.018631321302724 28.571397825278698 21.114591746088458 68-69 21.173108604998497 28.80423286188783 28.901159896235225 21.121498636878446 70-71 21.38484746033668 28.392675136329622 28.996154842781763 21.226322560551942 72-73 21.401740933935397 28.72518613849227 28.63337399419614 21.239698933376193 74-75 21.137024196277807 28.863464154157935 28.682958509039224 21.316553140525034 76-77 21.2673219879528 28.376239045475867 29.055568938785648 21.300870027785688 78-79 21.23502082263379 28.354661268652414 28.91955352834778 21.490764380366016 80-81 21.187744952464556 28.769476016872503 28.678175803779776 21.364603226883165 82-83 21.44493778713944 28.4269053033958 28.874677777980253 21.253479131484507 84-85 21.335133779329986 28.523342814619916 28.714955370582558 21.426568035467536 86-87 21.11989628723559 28.817052319407498 28.94635630646423 21.11669508689268 88-89 21.375179328225062 28.472339290709332 28.83121306133499 21.32126831973062 90-91 21.43813021311692 28.484833788831143 28.867014216979666 21.210021781072275 92-93 21.456379055452043 28.642485784733378 28.708662023835913 21.192473135978666 94-95 21.313580831564213 28.38782867902374 28.86624488928749 21.432345600124556 96-97 21.472289617361824 28.640633704746875 28.7978248184294 21.089251859461896 98-99 22.269180239362512 30.53250213490918 29.15341931573647 18.04489830999184 100 28.338675755965596 41.00588747684304 30.655436767191368 0.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4447.0 1 3966.0 2 3165.5 3 2914.0 4 3460.5 5 4721.5 6 5898.5 7 7345.0 8 8565.5 9 8730.0 10 8361.0 11 8109.5 12 8906.5 13 11419.0 14 17328.5 15 24512.5 16 29175.5 17 30546.0 18 28728.5 19 24752.5 20 20634.0 21 17373.0 22 16214.5 23 17868.5 24 21676.0 25 27636.0 26 36249.0 27 46936.0 28 58555.0 29 72135.0 30 87445.5 31 102706.0 32 123583.0 33 147696.5 34 163412.5 35 178201.5 36 196472.5 37 206768.5 38 212606.5 39 212517.5 40 208861.0 41 203703.0 42 195877.5 43 195519.0 44 195996.5 45 191392.5 46 183217.5 47 175878.0 48 170552.0 49 163606.0 50 156496.5 51 148276.0 52 138627.5 53 129174.0 54 119179.0 55 108497.5 56 96984.5 57 85272.5 58 73752.0 59 62728.5 60 52475.5 61 42224.0 62 32375.5 63 24772.5 64 19500.5 65 14707.0 66 10138.0 67 7060.0 68 5033.5 69 3575.0 70 2538.5 71 1806.5 72 1277.0 73 861.0 74 627.0 75 415.5 76 245.5 77 152.5 78 98.0 79 61.5 80 41.0 81 34.5 82 26.0 83 19.5 84 16.0 85 12.5 86 8.5 87 6.5 88 4.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.004946609223042835 3 1.5739211164227202E-4 4 6.74537621324023E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.2489780643675513E-5 26-27 4.3867537583512823E-4 28-29 0.0015419860284810445 30-31 7.552907553764302E-4 32-33 7.687745484975771E-4 34-35 9.644668841218002E-4 36-37 0.0 38-39 1.4946163917568917E-4 40-41 8.472910017463481E-4 42-43 3.280131552018845E-4 44-45 0.0 46-47 0.0 48-49 0.0015427623974624838 50-51 0.0035667737559738847 52-53 0.0 54-55 0.0037810702539183383 56-57 0.040079783779913364 58-59 0.05917285137251352 60-61 0.03134979995006387 62-63 0.0068901576070956 64-65 0.0 66-67 0.0 68-69 4.086298260544556E-5 70-71 0.003741064240285105 72-73 0.00843358530592516 74-75 0.005987242502571117 76-77 0.007308094804044901 78-79 0.010656660826797176 80-81 0.012042344888929978 82-83 0.04574512144105493 84-85 0.02646717374746747 86-87 0.0013172229814974589 88-89 0.0018627125005704558 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 57.0 20-21 229.0 22-23 572.0 24-25 1042.0 26-27 2170.0 28-29 5077.0 30-31 12305.0 32-33 15048.0 34-35 19839.0 36-37 32141.0 38-39 41197.0 40-41 39696.0 42-43 43109.0 44-45 52372.0 46-47 53781.0 48-49 51372.0 50-51 47337.0 52-53 39641.0 54-55 41517.0 56-57 43675.0 58-59 46513.0 60-61 45670.0 62-63 42053.0 64-65 42931.0 66-67 45269.0 68-69 48574.0 70-71 48336.0 72-73 42888.0 74-75 42760.0 76-77 44867.0 78-79 46917.0 80-81 48045.0 82-83 44032.0 84-85 41670.0 86-87 42606.0 88-89 44921.0 90-91 46853.0 92-93 45898.0 94-95 47962.0 96-97 119115.0 98-99 908063.0 100-101 2019371.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.04056017224343 #Duplication Level Percentage of deduplicated Percentage of total 1 92.29810063262977 82.1827458316345 2 5.772482845696401 10.279702123309468 3 1.165688074527567 3.1138055742611526 4 0.37818919336437923 1.3469671051301286 5 0.16033296957791218 0.7138068712648281 6 0.07559021434999783 0.4038357017558256 7 0.04417034917586024 0.2753066843535531 8 0.02498266188741281 0.1779576167239194 9 0.01681115893817802 0.13471875080999893 >10 0.06101753643519128 0.8449717129956849 >50 9.846000316397483E-4 0.06596142396406261 >100 0.001471564718804811 0.31084952162406126 >500 1.0691920061798057E-4 0.0626425573932759 >1k 7.127946636121553E-5 0.08672852477958727 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.2484587377467432E-5 0.0 0.0 0.0 0.0 6 2.2484587377467432E-5 0.0 0.0 0.0 0.0 7 4.4969174754934864E-5 0.0 0.0 0.0 0.0 8 4.4969174754934864E-5 0.0 0.0 0.0 0.0 9 4.4969174754934864E-5 2.2484587377467432E-5 0.0 2.2484587377467432E-5 0.0 10-11 4.4969174754934864E-5 2.2484587377467432E-5 0.0 2.2484587377467432E-5 0.0 12-13 6.745376213240229E-5 2.2484587377467432E-5 0.0 2.2484587377467432E-5 0.0 14-15 7.869605582113601E-5 2.2484587377467432E-5 0.0 2.2484587377467432E-5 0.0 16-17 1.0118064319860343E-4 2.2484587377467432E-5 0.0 4.4969174754934864E-5 0.0 18-19 1.2366523057607087E-4 2.2484587377467432E-5 0.0 5.6211468443668575E-5 0.0 20-21 1.3490752426480458E-4 2.2484587377467432E-5 0.0 7.869605582113601E-5 0.0 22-23 1.3490752426480458E-4 2.2484587377467432E-5 0.0 8.993834950986973E-5 0.0 24-25 1.6863440533100574E-4 2.2484587377467432E-5 0.0 8.993834950986973E-5 0.0 26-27 1.7987669901973945E-4 2.2484587377467432E-5 0.0 1.7987669901973945E-4 0.0 28-29 2.248458737746743E-4 2.2484587377467432E-5 0.0 5.621146844366858E-4 0.0 30-31 2.4733046115214173E-4 2.2484587377467432E-5 0.0 0.0021922472693030742 0.0 32-33 2.4733046115214173E-4 2.2484587377467432E-5 0.0 0.005519966201168254 0.0 34-35 2.4733046115214173E-4 3.3726881066201146E-5 0.0 0.009949429914529337 2.2484587377467432E-5 36-37 2.4733046115214173E-4 4.4969174754934864E-5 0.0 0.017335616868027388 2.2484587377467432E-5 38-39 2.4733046115214173E-4 4.4969174754934864E-5 0.0 0.0373806265150396 2.2484587377467432E-5 40-41 2.4733046115214173E-4 4.4969174754934864E-5 0.0 0.06005633288521551 2.2484587377467432E-5 42-43 2.5857275484087545E-4 4.4969174754934864E-5 0.0 0.07360329678013963 2.2484587377467432E-5 44-45 2.6981504852960917E-4 6.745376213240229E-5 0.0 0.0894324462938767 2.2484587377467432E-5 46-47 2.810573422183429E-4 8.993834950986973E-5 0.0 0.10635209829542094 3.3726881066201146E-5 48-49 2.922996359070766E-4 8.993834950986973E-5 0.0 0.121506710187834 4.4969174754934864E-5 50-51 2.922996359070766E-4 8.993834950986973E-5 0.0 0.13832518154617962 4.4969174754934864E-5 52-53 2.922996359070766E-4 8.993834950986973E-5 0.0 0.18000036425031551 5.6211468443668575E-5 54-55 2.922996359070766E-4 8.993834950986973E-5 0.0 0.22548668451493215 6.745376213240229E-5 56-57 3.1478422328454404E-4 8.993834950986973E-5 0.0 0.28448624179340665 7.869605582113601E-5 58-59 3.1478422328454404E-4 8.993834950986973E-5 0.0 0.33300798135398135 8.993834950986973E-5 60-61 3.372688106620115E-4 1.1242293688733715E-4 0.0 0.3503435982220088 1.1242293688733715E-4 62-63 3.372688106620115E-4 1.1242293688733715E-4 0.0 0.36978152400982933 1.1242293688733715E-4 64-65 3.372688106620115E-4 1.3490752426480458E-4 0.0 0.37892150877876984 1.1242293688733715E-4 66-67 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.38703844482203564 1.1242293688733715E-4 68-69 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.39425599737020267 1.1242293688733715E-4 70-71 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.40090019294024426 1.1242293688733715E-4 72-73 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.4063302207919027 1.1242293688733715E-4 74-75 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.41020881211451576 1.1242293688733715E-4 76-77 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.4138063460949106 1.1242293688733715E-4 78-79 3.597533980394789E-4 1.3490752426480458E-4 0.0 0.4163133775874982 1.1242293688733715E-4 80-81 3.8223798541694634E-4 1.3490752426480458E-4 0.0 0.4174713338374378 1.1242293688733715E-4 82-83 4.384494538606149E-4 1.3490752426480458E-4 0.0 0.4182807789830266 1.2366523057607087E-4 84-85 4.496917475493486E-4 1.3490752426480458E-4 0.0 0.41866301696844355 1.3490752426480458E-4 86-87 4.496917475493486E-4 1.3490752426480458E-4 0.0 0.4187641976116422 1.3490752426480458E-4 88 4.496917475493486E-4 1.3490752426480458E-4 0.0 0.4187754399053309 1.3490752426480458E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8245 0.0 54.612225 1 GTATCAA 16065 0.0 36.737625 1 TGCGCGA 165 1.900241E-5 32.794323 94 TCAACGC 21340 0.0 30.535799 4 TATCAAC 21575 0.0 30.243834 2 ATCAACG 21530 0.0 30.227987 3 CAACGCA 21660 0.0 30.027172 5 ACCGAAC 1005 0.0 29.154726 8 AACGCAG 22375 0.0 29.104536 6 GGACCGA 1020 0.0 27.9168 6 CCGAACC 1045 0.0 27.248934 9 GACCGAA 1100 0.0 25.51132 7 ACGCAGA 25510 0.0 25.47926 7 AGGACCG 1220 0.0 25.369865 5 CGCAGAG 25815 0.0 25.178226 8 GCAGAGT 28935 0.0 22.5061 9 TGGTATC 3490 0.0 20.931658 2 GAGTACT 15200 0.0 20.471254 12-13 ACATGGG 24720 0.0 20.300499 3 GTGGTAT 3620 0.0 20.293978 1 >>END_MODULE