FastQCFastQC Report
Fri 27 May 2016
SRR2139179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062889
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.051.567941
GTATCAA124250.036.4135931
TCAACGC155400.031.141324
ATCAACG156650.030.8656313
CAACGCA158550.030.522625
AACGCAG163600.029.6585646
TATCAAC164650.029.6005442
ACGCAGA187350.025.8533297
CGCAGAG191400.025.3062748
GCAGAGT221700.021.9437129
GGACCGA6650.021.1398546
TACATGG174000.021.1053922
ACATGGG174400.020.3473683
GAGTACT129200.019.766812-13
GTACATG173550.019.2699431
CAGAGTA223250.018.89093210-11
AGAGTAC209350.018.42574910-11
CATGGGG113200.018.1388724
GTACTTT147950.017.0027414-15
ACCGAAC5550.016.886498
Produced by FastQC (version 0.11.5)