##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5062889
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.300557843555328	18.0	18.0	30.0	18.0	33.0
2	28.678113424963495	29.0	27.0	32.0	25.0	33.0
3	30.639201056945947	31.0	29.0	33.0	27.0	33.0
4	31.46434910976717	33.0	31.0	33.0	29.0	33.0
5	32.65700255328529	33.0	33.0	33.0	32.0	34.0
6	36.68942554340022	38.0	37.0	38.0	34.0	38.0
7	37.058486172618046	38.0	38.0	38.0	35.0	38.0
8	37.25054983429421	38.0	38.0	38.0	36.0	38.0
9	37.405599451222415	38.0	38.0	38.0	37.0	38.0
10-11	37.44819331413349	38.0	38.0	38.0	37.0	38.0
12-13	37.491380316653206	38.0	38.0	38.0	37.0	38.0
14-15	37.49298868689399	38.0	38.0	38.0	37.0	38.0
16-17	37.51549668578552	38.0	38.0	38.0	37.0	38.0
18-19	37.51287229484984	38.0	38.0	38.0	37.0	38.0
20-21	37.51390412516085	38.0	38.0	38.0	37.0	38.0
22-23	37.52217041795579	38.0	38.0	38.0	37.5	38.0
24-25	37.52982412840244	38.0	38.0	38.0	38.0	38.0
26-27	37.49283728012571	38.0	38.0	38.0	37.0	38.0
28-29	37.46834342426376	38.0	38.0	38.0	37.0	38.0
30-31	37.465867904508926	38.0	38.0	38.0	37.0	38.0
32-33	37.454117355302785	38.0	38.0	38.0	37.0	38.0
34-35	37.42224005875288	38.0	38.0	38.0	37.0	38.0
36-37	37.39512259708631	38.0	38.0	38.0	37.0	38.0
38-39	37.3801776342269	38.0	38.0	38.0	37.0	38.0
40-41	37.37677468611325	38.0	38.0	38.0	37.0	38.0
42-43	37.335587822384355	38.0	38.0	38.0	37.0	38.0
44-45	37.289964293737384	38.0	38.0	38.0	37.0	38.0
46-47	37.25503042167246	38.0	38.0	38.0	37.0	38.0
48-49	37.20910351129049	38.0	38.0	38.0	37.0	38.0
50-51	37.1728479329908	38.0	38.0	38.0	37.0	38.0
52-53	37.132622681663356	38.0	38.0	38.0	36.5	38.0
54-55	37.11560522004794	38.0	38.0	38.0	36.0	38.0
56-57	37.07063158816203	38.0	38.0	38.0	36.0	38.0
58-59	37.03869436480882	38.0	38.0	38.0	36.0	38.0
60-61	37.0628382756958	38.0	38.0	38.0	36.0	38.0
62-63	37.076076889189	38.0	38.0	38.0	36.0	38.0
64-65	37.0838501459089	38.0	38.0	38.0	36.0	38.0
66-67	37.072551863164776	38.0	38.0	38.0	36.0	38.0
68-69	37.034803648314366	38.0	38.0	38.0	36.0	38.0
70-71	37.03047419319594	38.0	38.0	38.0	36.0	38.0
72-73	37.04126335005253	38.0	38.0	38.0	36.0	38.0
74-75	37.0551331558959	38.0	38.0	38.0	36.0	38.0
76-77	37.03935603558392	38.0	38.0	38.0	36.0	38.0
78-79	36.996211965570296	38.0	38.0	38.0	36.0	38.0
80-81	36.94515604373374	38.0	38.0	38.0	36.0	38.0
82-83	36.90053439488676	38.0	38.0	38.0	35.5	38.0
84-85	36.87553295950032	38.0	38.0	38.0	35.0	38.0
86-87	36.89687781865254	38.0	38.0	38.0	35.0	38.0
88-89	36.85438900914677	38.0	38.0	38.0	35.0	38.0
90-91	36.86878775846903	38.0	38.0	38.0	35.0	38.0
92-93	36.85447055681644	38.0	38.0	38.0	35.0	38.0
94-95	36.86896605683749	38.0	38.0	38.0	35.0	38.0
96-97	36.860454873166574	38.0	38.0	38.0	35.0	38.0
98-99	36.8407079678167	38.0	38.0	38.0	35.0	38.0
100	35.19819573998513	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	10.0
20	20.0
21	71.0
22	239.0
23	752.0
24	1909.0
25	4069.0
26	7406.0
27	12876.0
28	20325.0
29	29728.0
30	39048.0
31	50078.0
32	64924.0
33	87742.0
34	135540.0
35	262342.0
36	943846.0
37	3401961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.14750609780305	27.641076073364435	13.607527243832523	21.603890584999988
2	13.823455048966741	21.698310361038356	42.45679114766662	22.02144344232828
3	17.24561840815693	28.044064195796626	31.35391553355142	23.356401862495026
4	11.99017074780257	18.884137719350395	40.45401727526034	28.671674257586687
5	12.564980192139311	38.331711400348695	36.14924206317776	12.954066344334233
6	27.79889110742898	39.71325462596553	19.779023399485947	12.708830867119545
7	24.888497456689255	33.55110096231618	24.769138726920538	16.791262854074027
8	23.182811236825458	37.85297287773838	22.118399198560347	16.845816686875814
9	25.459574563060734	17.05459076823529	22.064793441057073	35.4210412276469
10-11	23.30890525152734	27.39620600017105	29.930569285639088	19.364319462662525
12-13	24.491253906613398	25.0917707261605	29.967642980124587	20.449332387101514
14-15	21.68264996526687	26.16280941573082	27.65054695056518	24.503993668437133
16-17	20.954449129736005	29.347157324602613	28.213358025427777	21.485035520233605
18-19	20.95952528289678	28.437933361762425	30.1244901873219	20.478051168018894
20-21	21.938278438833713	27.59528661659244	29.905512098747387	20.56092284582646
22-23	21.58778490248317	27.583967684509158	29.726350408758368	21.10189700424931
24-25	21.30792662961912	27.723493987387187	29.88430897152413	21.08427041146956
26-27	21.24438420900818	27.862838379807254	29.806168776004522	21.086608635180042
28-29	21.21128575738414	27.886616718511124	29.827742798126383	21.07435472597835
30-31	21.427079973408436	27.780934726531907	29.784619602841666	21.007365697217985
32-33	21.214896196744824	27.968187972701287	29.691721011767324	21.125194818786568
34-35	21.40415906122071	27.94180239233252	29.70543129187466	20.94860725457211
36-37	21.36697601549456	27.889146945479464	29.589578342710322	21.154298696315653
38-39	21.19775410826639	27.957790359238793	29.7467946988869	21.097660833607918
40-41	21.38852112081108	27.79086176429828	29.691085244597424	21.12953187029321
42-43	21.38127078578457	27.865790440177324	29.458633653578975	21.294305120459132
44-45	21.354412753367797	28.087764565807866	29.28685251134336	21.270970169480975
46-47	21.48280563069152	28.00301241459301	29.024653843905867	21.4895281108096
48-49	21.470903561296158	28.045657856762567	29.006377430086346	21.477061151854933
50-51	21.37881435548274	28.258077846604017	28.861959551412973	21.501148246500268
52-53	21.566048414872782	28.14422151831434	28.840639143087	21.44909092372588
54-55	21.533592781536505	28.148081768856404	28.78687695626292	21.531448493344172
56-57	21.589510111287456	28.244763783164718	28.66872879220466	21.49699731334317
58-59	21.432869406389525	28.307216448099993	28.836699111504853	21.423215034005626
60-61	21.555693395523985	28.384187929588116	28.730314813090494	21.3298038617974
62-63	21.482133952917135	28.3998619975088	28.756059406408284	21.361944643165774
64-65	21.375295358591075	28.333696066616753	28.789804875469304	21.501203699322865
66-67	21.466377691890383	28.484080243537058	28.537352851416053	21.512189213156503
68-69	21.483717162691253	28.27398019715666	28.68488215868882	21.557420481463268
70-71	21.488857391502314	28.064102587640864	28.73332005302332	21.713719967833498
72-73	21.548391377882435	28.134062844655645	28.718794870126125	21.5987509073358
74-75	21.41742421332414	28.298208870205393	28.643402809332464	21.640964107138004
76-77	21.584701068612766	28.096819461307565	28.628276683103525	21.690202786976144
78-79	21.593539390123244	28.16037535287269	28.4949504986917	21.751134758312364
80-81	21.644063867396913	28.178325460990855	28.448255044577742	21.729355627034494
82-83	21.71561810612076	28.10630025239985	28.559999697183674	21.618081944295717
84-85	21.802147232643836	28.1325977016257	28.427457121755236	21.637797943975233
86-87	21.593402269504537	28.251822561662276	28.54939432230391	21.605380846529275
88-89	21.749802580619836	28.168562177919533	28.455581520237438	21.6260537212232
90-91	21.824785853292852	28.194970375836647	28.463769620862372	21.516474150008126
92-93	21.81200678429703	28.182939627713438	28.428910952432023	21.576142635557506
94-95	21.683063791027656	28.262626515271723	28.467750150918157	21.586559542782467
96-97	21.743354386663203	28.374544613458006	28.56506198761571	21.317039012263084
98-99	22.71512924648262	30.29774460721534	28.75591556916165	18.23121057714039
100	21.705270867892796	31.139929236674053	22.817889845812086	24.336910049621068
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1376.0
1	1370.0
2	1776.5
3	2279.5
4	2822.0
5	3849.0
6	4744.0
7	6053.0
8	7236.0
9	7454.5
10	7155.5
11	6893.0
12	7543.5
13	9558.5
14	13738.0
15	18441.5
16	21597.0
17	22917.0
18	22083.5
19	20141.5
20	18653.0
21	18268.5
22	19877.0
23	23890.5
24	29878.0
25	38545.0
26	50594.5
27	64012.5
28	77216.0
29	91398.5
30	104973.5
31	117350.5
32	131214.0
33	144951.0
34	157127.0
35	169060.0
36	181428.0
37	192047.0
38	197661.0
39	201014.5
40	204229.5
41	205386.0
42	205784.5
43	208242.0
44	209709.5
45	208785.5
46	205485.5
47	200867.5
48	196561.0
49	190703.5
50	184189.0
51	176871.5
52	167814.5
53	156671.5
54	144750.0
55	132641.5
56	119849.0
57	106698.0
58	93976.0
59	81088.5
60	67522.5
61	54347.0
62	42078.0
63	32159.5
64	24496.0
65	18258.5
66	13233.0
67	9611.5
68	7096.0
69	5072.5
70	3710.0
71	2706.0
72	1811.5
73	1178.0
74	775.0
75	503.5
76	323.0
77	178.5
78	110.0
79	68.0
80	42.0
81	32.5
82	25.5
83	20.5
84	17.0
85	11.0
86	5.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00531317198540201
3	5.9254706156899745E-5
4	8.295658861965965E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.87637153171461E-6
24-25	3.950823702295162E-5
26-27	5.828139621283536E-4
28-29	0.0013241333078124755
30-31	6.528164728147933E-4
32-33	7.730254551336026E-4
34-35	0.0011922176798728618
36-37	0.0
38-39	2.1057446820671474E-4
40-41	7.97380554411634E-4
42-43	3.759015796197136E-4
44-45	0.0
46-47	0.0
48-49	0.0016565832148112295
50-51	0.0038149062153124656
52-53	0.0
54-55	0.0038403686753928374
56-57	0.03919663455072604
58-59	0.05682001747207406
60-61	0.030124115951813225
62-63	0.007040898127253529
64-65	0.0
66-67	0.0
68-69	6.81071325194531E-5
70-71	0.003821003101667713
72-73	0.008143172280641556
74-75	0.005925423509691581
76-77	0.00711648527904188
78-79	0.010449529191983886
80-81	0.011469122105103036
82-83	0.0428643177134474
84-85	0.025434557364728348
86-87	0.001220255949930657
88-89	0.0017741006222060274
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	179.0
22-23	365.0
24-25	442.0
26-27	1267.0
28-29	3296.0
30-31	9551.0
32-33	11608.0
34-35	15775.0
36-37	26121.0
38-39	32521.0
40-41	32056.0
42-43	34778.0
44-45	41801.0
46-47	43494.0
48-49	41997.0
50-51	38306.0
52-53	33470.0
54-55	35885.0
56-57	37957.0
58-59	40369.0
60-61	41082.0
62-63	39201.0
64-65	40776.0
66-67	44262.0
68-69	46258.0
70-71	49037.0
72-73	41407.0
74-75	39646.0
76-77	42041.0
78-79	44935.0
80-81	45487.0
82-83	42020.0
84-85	39881.0
86-87	40835.0
88-89	44100.0
90-91	45887.0
92-93	44937.0
94-95	49499.0
96-97	148665.0
98-99	331292.0
100-101	3320364.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.4608063661859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.45653265398003	78.2094475435234
2	6.83943070809285	11.826853882147233
3	1.5487149836066192	4.017094389420681
4	0.554648417598413	1.918213977411512
5	0.24294503768280823	1.0502611930359511
6	0.12197142052620356	0.6327448423394831
7	0.07210578023391889	0.43640267318813314
8	0.04368985293874024	0.30219679320828463
9	0.030092332531821336	0.2341626602526613
>10	0.08765486014588215	1.1371298656660134
>50	0.001553876544209675	0.09163668277007324
>100	5.912395272391704E-4	0.10850259397646521
>500	6.883659124711378E-5	0.03535290306012751
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.975156871896658E-5	0.0
2	0.0	0.0	0.0	1.975156871896658E-5	0.0
3	0.0	0.0	0.0	1.975156871896658E-5	0.0
4	0.0	3.950313743793316E-5	0.0	9.875784359483291E-5	0.0
5	0.0	5.9254706156899745E-5	0.0	9.875784359483291E-5	0.0
6	0.0	5.9254706156899745E-5	0.0	9.875784359483291E-5	0.0
7	0.0	5.9254706156899745E-5	0.0	9.875784359483291E-5	0.0
8	0.0	7.900627487586633E-5	0.0	9.875784359483291E-5	0.0
9	0.0	1.7776411847069925E-4	0.0	9.875784359483291E-5	0.0
10-11	0.0	1.7776411847069925E-4	0.0	1.0863362795431619E-4	0.0
12-13	0.0	1.7776411847069925E-4	0.0	1.5801254975173265E-4	0.0
14-15	0.0	2.567703933465656E-4	0.0	1.6788833411121595E-4	0.0
16-17	0.0	2.567703933465656E-4	0.0	1.8763990283018255E-4	0.0
18-19	0.0	2.6664617770604887E-4	0.0	1.9751568718966582E-4	0.0
20-21	0.0	2.7652196206553217E-4	0.0	2.468946089870823E-4	0.0
22-23	0.0	3.160250995034653E-4	0.0	2.7652196206553217E-4	0.0
24-25	0.0	3.160250995034653E-4	0.0	3.259008838629486E-4	0.0
26-27	0.0	3.160250995034653E-4	0.0	4.147829430982982E-4	0.0
28-29	0.0	3.259008838629486E-4	0.0	9.184479454319461E-4	0.0
30-31	0.0	3.7527980566036504E-4	0.0	0.002617082855263072	0.0
32-33	0.0	3.7527980566036504E-4	0.0	0.005767458065938242	0.0
34-35	0.0	4.147829430982982E-4	0.0	0.008917833276613413	0.0
36-37	0.0	4.5428608053623136E-4	0.0	0.014724794479989587	0.0
38-39	0.0	4.5428608053623136E-4	0.0	0.03190865926549051	0.0
40-41	0.0	5.135407866931311E-4	0.0	0.050820786313901016	0.0
42-43	0.0	5.332923554120977E-4	0.0	0.06261247283912406	0.0
44-45	0.0	6.12298630287964E-4	0.0	0.07628055839264894	0.0
46-47	0.0	6.715533364448638E-4	0.0	0.09079796140108938	0.0
48-49	0.0	6.715533364448638E-4	0.0	0.10513760029105912	0.0
50-51	0.0	7.505596113207302E-4	0.0	0.12013891673311423	0.0
52-53	0.0	7.703111800396967E-4	0.0	0.1569755923939869	0.0
54-55	0.0	7.703111800396967E-4	0.0	0.19998463327953664	0.0
56-57	0.0	7.703111800396967E-4	0.0	0.2607997133652348	0.0
58-59	0.0	7.703111800396967E-4	0.0	0.3021594982627508	0.0
60-61	0.0	7.8018696439918E-4	0.0	0.3231159126735743	0.0
62-63	0.0	7.900627487586633E-4	0.0	0.34022077118419936	0.0
64-65	0.0	8.49317454915563E-4	0.0	0.34593885032834015	0.0
66-67	0.0	8.690690236345296E-4	0.0	0.3524963711430371	0.0
68-69	0.0	8.789448079940129E-4	0.0	0.3587279910738711	0.0
70-71	0.0	8.986963767129794E-4	0.0	0.3634584917820636	0.0
72-73	0.0	9.085721610724627E-4	0.0	0.36791247052819054	0.0
74-75	0.0	9.381995141509126E-4	0.0	0.37121098250425794	0.0
76-77	0.0	9.678268672293625E-4	0.0	0.3740058294779917	0.0
78-79	0.0	9.875784359483291E-4	0.0	0.37581309801577717	0.0
80-81	1.975156871896658E-5	9.875784359483291E-4	0.0	0.3764747755678626	0.0
82-83	3.950313743793316E-5	9.875784359483291E-4	0.0	0.37716608047302635	0.0
84-85	3.950313743793316E-5	9.875784359483291E-4	0.0	0.37744260243509187	0.0
86-87	3.950313743793316E-5	9.875784359483291E-4	0.0	0.3775512360630462	0.0
88	3.950313743793316E-5	0.001086336279543162	0.0	0.37757098763176516	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6320	0.0	51.56794	1
GTATCAA	12425	0.0	36.413593	1
TCAACGC	15540	0.0	31.14132	4
ATCAACG	15665	0.0	30.865631	3
CAACGCA	15855	0.0	30.52262	5
AACGCAG	16360	0.0	29.658564	6
TATCAAC	16465	0.0	29.600544	2
ACGCAGA	18735	0.0	25.853329	7
CGCAGAG	19140	0.0	25.306274	8
GCAGAGT	22170	0.0	21.943712	9
GGACCGA	665	0.0	21.139854	6
TACATGG	17400	0.0	21.105392	2
ACATGGG	17440	0.0	20.347368	3
GAGTACT	12920	0.0	19.7668	12-13
GTACATG	17355	0.0	19.269943	1
CAGAGTA	22325	0.0	18.890932	10-11
AGAGTAC	20935	0.0	18.425749	10-11
CATGGGG	11320	0.0	18.138872	4
GTACTTT	14795	0.0	17.00274	14-15
ACCGAAC	555	0.0	16.88649	8
>>END_MODULE