FastQCFastQC Report
Fri 27 May 2016
SRR2139178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4843792
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.048.7364651
GTATCAA125800.035.7842251
TCAACGC153550.031.6260664
ATCAACG153750.031.5849253
CAACGCA156950.030.9675355
TATCAAC161900.030.361362
AACGCAG162650.029.934226
ACGCAGA185250.026.1683487
CGCAGAG190800.025.517828
GCAGAGT220250.022.02919
TACATGG170500.021.523382
ACATGGG170250.020.8596023
GTACATG172700.020.0510251
TATACAC75800.019.5539743
GAGTACT127200.019.4869412-13
CAGAGTA219300.018.7259410-11
AGAGTAC204900.018.54783410-11
CTTATAC53750.018.3845271
CATGGGG108400.018.1532254
TGGTATC31400.017.8869822
Produced by FastQC (version 0.11.5)