##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4843792
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.218256688148458	18.0	18.0	30.0	18.0	32.0
2	28.56693970343896	29.0	27.0	32.0	25.0	33.0
3	30.551976426733436	31.0	29.0	33.0	27.0	33.0
4	31.453152612663796	33.0	31.0	33.0	29.0	33.0
5	32.651837857612385	33.0	33.0	33.0	32.0	34.0
6	36.66356131724896	38.0	37.0	38.0	34.0	38.0
7	37.069624376934435	38.0	38.0	38.0	35.0	38.0
8	37.254099061231365	38.0	38.0	38.0	36.0	38.0
9	37.40664772558359	38.0	38.0	38.0	37.0	38.0
10-11	37.4573489117617	38.0	38.0	38.0	37.0	38.0
12-13	37.49666820953501	38.0	38.0	38.0	37.0	38.0
14-15	37.499482636744105	38.0	38.0	38.0	37.0	38.0
16-17	37.521008437191355	38.0	38.0	38.0	37.0	38.0
18-19	37.52117834539551	38.0	38.0	38.0	37.0	38.0
20-21	37.523726794198254	38.0	38.0	38.0	37.0	38.0
22-23	37.5320611564301	38.0	38.0	38.0	37.5	38.0
24-25	37.53757111245338	38.0	38.0	38.0	38.0	38.0
26-27	37.502243270084925	38.0	38.0	38.0	37.5	38.0
28-29	37.478307539453745	38.0	38.0	38.0	37.0	38.0
30-31	37.47533857572857	38.0	38.0	38.0	37.0	38.0
32-33	37.46071625298326	38.0	38.0	38.0	37.0	38.0
34-35	37.42809901364529	38.0	38.0	38.0	37.0	38.0
36-37	37.400220535489765	38.0	38.0	38.0	37.0	38.0
38-39	37.377033171292354	38.0	38.0	38.0	37.0	38.0
40-41	37.3842863056728	38.0	38.0	38.0	37.0	38.0
42-43	37.346227271371546	38.0	38.0	38.0	37.0	38.0
44-45	37.29747035790399	38.0	38.0	38.0	37.0	38.0
46-47	37.26013529384501	38.0	38.0	38.0	37.0	38.0
48-49	37.21210122352253	38.0	38.0	38.0	37.0	38.0
50-51	37.173030854238505	38.0	38.0	38.0	37.0	38.0
52-53	37.13490983917528	38.0	38.0	38.0	36.5	38.0
54-55	37.11629520316774	38.0	38.0	38.0	36.0	38.0
56-57	37.072041818341646	38.0	38.0	38.0	36.0	38.0
58-59	37.03990162691177	38.0	38.0	38.0	36.0	38.0
60-61	37.06007343740899	38.0	38.0	38.0	36.0	38.0
62-63	37.074509645764984	38.0	38.0	38.0	36.0	38.0
64-65	37.08184853837264	38.0	38.0	38.0	36.0	38.0
66-67	37.069432519957374	38.0	38.0	38.0	36.0	38.0
68-69	37.03306835691454	38.0	38.0	38.0	36.0	38.0
70-71	37.02884507793614	38.0	38.0	38.0	36.0	38.0
72-73	37.03898575900379	38.0	38.0	38.0	36.0	38.0
74-75	37.055165538667126	38.0	38.0	38.0	36.0	38.0
76-77	37.03757512024764	38.0	38.0	38.0	36.0	38.0
78-79	36.99786309888091	38.0	38.0	38.0	36.0	38.0
80-81	36.949917665303985	38.0	38.0	38.0	36.0	38.0
82-83	36.90857871736201	38.0	38.0	38.0	35.5	38.0
84-85	36.88356689731552	38.0	38.0	38.0	35.0	38.0
86-87	36.911185068431664	38.0	38.0	38.0	35.0	38.0
88-89	36.86401040414523	38.0	38.0	38.0	35.0	38.0
90-91	36.878467325715064	38.0	38.0	38.0	35.0	38.0
92-93	36.86397442247244	38.0	38.0	38.0	35.0	38.0
94-95	36.87875142812601	38.0	38.0	38.0	35.0	38.0
96-97	36.87068218420633	38.0	38.0	38.0	35.0	38.0
98-99	36.851324086709454	38.0	38.0	38.0	35.0	38.0
100	35.35137510319294	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	10.0
20	20.0
21	80.0
22	234.0
23	684.0
24	1686.0
25	3614.0
26	6870.0
27	12051.0
28	19275.0
29	28123.0
30	37134.0
31	46894.0
32	60580.0
33	82718.0
34	128861.0
35	251822.0
36	921438.0
37	3241696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.2197014240083	28.63904973623971	13.495686850302407	21.64556198944959
2	13.83207960285287	21.612517278150612	42.55356355727055	22.001839561725966
3	17.209867902889993	28.167662272375836	31.46380361638677	23.158666208347398
4	12.000553289180306	18.902732587906915	40.11278428328406	28.98392983962872
5	12.46866091690147	38.37053696773107	36.22073367312221	12.94006844224525
6	27.79784928832617	39.59373152274086	19.65709923134602	12.951319957586948
7	24.782216081945716	33.505195929139816	24.807712635059474	16.904875353854994
8	23.122545311607105	37.918453145799816	22.18668761994735	16.77231392264573
9	25.393286912402512	17.005333837621432	22.13480677948186	35.46657247049419
10-11	23.381681129164917	27.397037279883197	29.845449598166063	19.375831992785816
12-13	24.47574132002365	25.096876579341142	29.886811820160737	20.540570280474473
14-15	21.654614814178643	26.150080350271026	27.567100321401085	24.628204514149246
16-17	20.93081205799093	29.38895807251839	28.17161636998451	21.508613499506172
18-19	20.965227243448933	28.350143854236514	30.15928429627036	20.52534460604419
20-21	21.85448511046634	27.530948205703943	29.941111596044212	20.67345508778551
22-23	21.55523478330169	27.639426247793576	29.76312988932944	21.042209079575297
24-25	21.321031571468556	27.848099828324703	29.90240142039656	20.928467179810184
26-27	21.265742614004434	27.953360540992282	29.85500904593053	20.925887799072754
28-29	21.151994245389634	27.91394031430345	29.88946400791383	21.04460143239309
30-31	21.542761237096858	27.76110732480023	29.82267346362034	20.873457974482573
32-33	21.24863031962715	27.933955626553182	29.806866906086743	21.010547147732925
34-35	21.358644338358364	27.798307345592182	29.879107248732485	20.96394106731697
36-37	21.275288244507728	27.90318490745601	29.801377646480592	21.02014920155567
38-39	21.13300199314449	27.789753865924304	29.95630749217984	21.12093664875136
40-41	21.3035986042134	27.67163964735373	29.946482437332012	21.078279311100857
42-43	21.41428829185401	27.716730356746105	29.64969355695265	21.219287794447236
44-45	21.23189202609146	27.990183487263103	29.541672715901857	21.236251770743582
46-47	21.51154357116203	27.824855847653524	29.17363134683923	21.489969234345217
48-49	21.479839803570854	27.94924529404926	29.051586516997606	21.519328385382284
50-51	21.312615717115396	28.102981422156926	29.035227735261675	21.549175125466004
52-53	21.48852181211157	28.04342075382597	29.10754523515192	21.360512198910538
54-55	21.431134043192287	28.070372044320802	28.94117977323066	21.55731413925625
56-57	21.585197727598562	28.198925041690813	28.695624995328682	21.520252235381943
58-59	21.296396298479202	28.30001203454841	29.056679980100135	21.34691168687225
60-61	21.487653832515516	28.574464015828344	28.69777688468551	21.24010526697063
62-63	21.30749181609605	28.83017475101895	28.695180360260604	21.167153072624398
64-65	21.257594957387848	28.671230046378927	28.76607519492617	21.305099801307055
66-67	21.284495495528663	28.743193176675103	28.620681342039006	21.351629985757228
68-69	21.359761208259105	28.48690949721422	28.759402809217082	21.393926485309592
70-71	21.464160481959595	28.192096936770046	28.787699308428454	21.556043272841904
72-73	21.530701070334697	28.21777360232019	28.79145141583246	21.46007391151266
74-75	21.43225578282288	28.321729795165723	28.735348114233716	21.510666307777672
76-77	21.55324617331866	28.184081991227007	28.65506589299121	21.607605942463127
78-79	21.526249862741828	28.169189505748577	28.594842346281013	21.70971828522858
80-81	21.595839467204947	28.265780389182098	28.515746639495493	21.62263350411746
82-83	21.667495634097637	28.139088028274838	28.6696315208328	21.523784816794727
84-85	21.690099551413862	28.20094870972322	28.55008861285386	21.558863126009058
86-87	21.516799127765186	28.340963262634283	28.619660244986616	21.52257736461392
88-89	21.65735893375139	28.235492112211784	28.5245760279416	21.582572926095224
90-91	21.720631315640556	28.252664906532697	28.53030535555853	21.496398422268218
92-93	21.778250136325187	28.268925328195245	28.45388452145283	21.498940014026736
94-95	21.550517047034315	28.294959467011683	28.572678275220913	21.581845210733093
96-97	21.650197049314286	28.378060040369697	28.610459030476036	21.36128387983998
98-99	22.617910411296922	30.3101523276694	28.928407827712157	18.143529433321525
100	28.563336917649796	41.154132853265075	30.28253022908513	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1554.0
1	1600.0
2	2065.0
3	2583.0
4	3178.5
5	4235.5
6	5075.0
7	6358.0
8	7497.5
9	7660.0
10	7291.0
11	7002.0
12	7742.5
13	9773.5
14	14237.5
15	19551.5
16	23081.0
17	24211.5
18	23068.5
19	21048.0
20	19220.5
21	18487.0
22	19807.5
23	23291.0
24	29337.5
25	38132.0
26	48984.0
27	60936.5
28	73821.0
29	88051.0
30	101515.5
31	113919.5
32	127889.0
33	141579.5
34	152052.5
35	162965.0
36	174865.0
37	183908.0
38	191200.5
39	195871.0
40	198685.0
41	200315.0
42	201980.5
43	208192.5
44	213430.0
45	212462.5
46	206254.5
47	199149.0
48	192662.5
49	184954.5
50	177385.0
51	169204.0
52	159200.5
53	147716.5
54	135338.0
55	123080.0
56	110296.0
57	96943.5
58	84395.0
59	71721.5
60	59810.5
61	48846.5
62	38559.0
63	29744.0
64	22211.5
65	16305.0
66	11835.5
67	8600.5
68	6186.0
69	4333.5
70	3124.5
71	2289.5
72	1605.0
73	1101.5
74	769.5
75	521.5
76	326.5
77	180.0
78	109.5
79	77.0
80	55.5
81	44.0
82	29.0
83	15.5
84	10.0
85	8.0
86	5.5
87	5.5
88	6.0
89	3.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005099310622751761
3	1.4451487594843048E-4
4	6.606394329071107E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.510564527686583E-4
28-29	0.001187845223973898
30-31	4.963368787297002E-4
32-33	5.803586927627716E-4
34-35	9.145467282974163E-4
36-37	0.0
38-39	2.310156339830298E-4
40-41	8.778321986735216E-4
42-43	3.727874135040004E-4
44-45	0.0
46-47	0.0
48-49	0.0012042394483444774
50-51	0.0031054019848933793
52-53	0.0
54-55	0.003346125338444297
56-57	0.03776396570542884
58-59	0.056118286794258
60-61	0.028830055586653577
62-63	0.0061590162525844
64-65	0.0
66-67	0.0
68-69	3.603930879488876E-5
70-71	0.003210625809345256
72-73	0.007415732883823306
74-75	0.005587106600500724
76-77	0.006901353758993915
78-79	0.010077292707992208
80-81	0.011480003902944217
82-83	0.04251230698125167
84-85	0.024868520186350915
86-87	0.0012751808365823879
88-89	0.0017729127142689252
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	26.0
20-21	175.0
22-23	346.0
24-25	514.0
26-27	1312.0
28-29	3562.0
30-31	10342.0
32-33	12616.0
34-35	17126.0
36-37	27867.0
38-39	34122.0
40-41	32902.0
42-43	36176.0
44-45	43228.0
46-47	45330.0
48-49	43541.0
50-51	38942.0
52-53	34037.0
54-55	35743.0
56-57	38336.0
58-59	41030.0
60-61	41969.0
62-63	40058.0
64-65	43040.0
66-67	46961.0
68-69	48988.0
70-71	55747.0
72-73	41757.0
74-75	40243.0
76-77	41972.0
78-79	45030.0
80-81	45296.0
82-83	41623.0
84-85	39554.0
86-87	40914.0
88-89	43812.0
90-91	44817.0
92-93	44022.0
94-95	48145.0
96-97	140211.0
98-99	1066027.0
100-101	2346333.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.95105172111039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07064272422303	80.09758808512906
2	6.574820495919623	11.565247549872868
3	1.4487648894783929	3.8226118717872875
4	0.4644559648798752	1.6339756235731255
5	0.1932746318472494	0.8499353570988003
6	0.09403482048209279	0.4962276815883524
7	0.05011360612932265	0.30852810552279875
8	0.030276855854899115	0.2130305052197469
9	0.01772905709458006	0.14033602957427366
>10	0.054308281016928917	0.688148110995645
>50	9.129683942336056E-4	0.05634141701166983
>100	6.657046798139838E-4	0.12802966262635412
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.128996455669442E-5	0.0	0.0	0.0
3	0.0	4.128996455669442E-5	0.0	0.0	0.0
4	0.0	1.4451487594843048E-4	0.0	0.0	0.0
5	0.0	1.4451487594843048E-4	0.0	0.0	0.0
6	0.0	1.4451487594843048E-4	0.0	0.0	0.0
7	0.0	1.4451487594843048E-4	0.0	0.0	0.0
8	0.0	1.4451487594843048E-4	0.0	0.0	0.0
9	0.0	2.6838476961851376E-4	0.0	0.0	0.0
10-11	0.0	2.6838476961851376E-4	0.0	0.0	0.0
12-13	0.0	2.6838476961851376E-4	0.0	0.0	0.0
14-15	0.0	2.8902975189686096E-4	0.0	0.0	0.0
16-17	0.0	3.0967473417520817E-4	0.0	0.0	0.0
18-19	0.0	3.0967473417520817E-4	0.0	1.0322491139173605E-5	0.0
20-21	0.0	3.0967473417520817E-4	0.0	4.128996455669442E-5	0.0
22-23	0.0	3.5096469873190263E-4	0.0	4.128996455669442E-5	0.0
24-25	0.0	3.9225466328859704E-4	0.0	4.128996455669442E-5	0.0
26-27	0.0	4.1289964556694424E-4	0.0	1.1354740253090968E-4	0.0
28-29	0.0	4.2322213670611784E-4	0.0	3.716096810102498E-4	0.0
30-31	0.0	5.161245569586803E-4	0.0	0.0020644982278347213	0.0
32-33	0.0	5.161245569586803E-4	0.0	0.00503737567591672	0.0
34-35	0.0	5.883819949328955E-4	0.0	0.008650247574627483	0.0
36-37	0.0	6.606394329071107E-4	0.0	0.014626969944209001	0.0
38-39	0.0	7.122518886029789E-4	0.0	0.03309390659219058	0.0
40-41	0.0	8.051543088555413E-4	0.0	0.05336727918952754	0.0
42-43	0.0	8.464442734122357E-4	0.0	0.06428847481477322	0.0
44-45	0.0	9.187017113864509E-4	0.0	0.07696449393367841	0.0
46-47	0.0	9.496691848039718E-4	0.0	0.08988825283992377	0.0
48-49	0.0	9.599916759431454E-4	0.0	0.10294620413097838	0.0
50-51	0.0	0.0010425716050565343	0.0	0.11769704396885745	0.0
52-53	0.0	0.0010528940961957078	0.0	0.15729411997872741	0.0
54-55	0.0	0.0010632165873348813	0.0	0.204065327330323	0.0
56-57	0.0	0.001073539078474055	0.0	0.2710892622969773	0.0
58-59	0.0	0.0010941840607524022	0.0	0.3159714537701041	0.0
60-61	0.0	0.0011148290430307494	0.0	0.33507838486871444	0.0
62-63	0.0	0.0011561190075874439	0.0	0.35035567175469134	0.0
64-65	0.0	0.0013832138126492633	0.0	0.3564356190356646	0.0
66-67	0.0	0.0013832138126492633	0.0	0.36164847706094727	0.0
68-69	0.0	0.0015586961620152147	0.0	0.3667168202102815	0.0
70-71	0.0	0.0016619210734069505	0.0	0.37179548585075495	0.0
72-73	0.0	0.0016825660556852979	0.0	0.37621351205832126	0.0
74-75	0.0	0.0017238560202419923	0.0	0.37945477427602176	0.0
76-77	0.0	0.0018477259139120755	0.0	0.3827889389139748	0.0
78-79	0.0	0.0018786933873295964	0.0	0.3850702094557322	0.0
80-81	0.0	0.0018786933873295964	0.0	0.3858134288177527	0.0
82-83	0.0	0.00188901587846877	0.0	0.3862572959367372	0.0
84-85	0.0	0.0018993383696079436	0.0	0.3864740682506598	0.0
86-87	0.0	0.0019199833518862908	0.0	0.38661858312660824	0.0
88	0.0	0.0020851432101130682	0.0	0.38661858312660824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6360	0.0	48.736465	1
GTATCAA	12580	0.0	35.784225	1
TCAACGC	15355	0.0	31.626066	4
ATCAACG	15375	0.0	31.584925	3
CAACGCA	15695	0.0	30.967535	5
TATCAAC	16190	0.0	30.36136	2
AACGCAG	16265	0.0	29.93422	6
ACGCAGA	18525	0.0	26.168348	7
CGCAGAG	19080	0.0	25.51782	8
GCAGAGT	22025	0.0	22.0291	9
TACATGG	17050	0.0	21.52338	2
ACATGGG	17025	0.0	20.859602	3
GTACATG	17270	0.0	20.051025	1
TATACAC	7580	0.0	19.553974	3
GAGTACT	12720	0.0	19.48694	12-13
CAGAGTA	21930	0.0	18.72594	10-11
AGAGTAC	20490	0.0	18.547834	10-11
CTTATAC	5375	0.0	18.384527	1
CATGGGG	10840	0.0	18.153225	4
TGGTATC	3140	0.0	17.886982	2
>>END_MODULE