FastQCFastQC Report
Fri 27 May 2016
SRR2139177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609030
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.050.7974851
GTATCAA91000.035.513531
TCAACGC112600.030.6832394
CAACGCA114900.030.032375
ATCAACG115050.030.0298373
AACGCAG118100.029.1472726
TATCAAC121250.028.460472
CGAGACC40200.027.21952686-87
AGACCGA42450.026.3717188-89
GAGACCG42300.025.04744788-89
GACCGAG43400.024.84130590-91
ACGCAGA139450.024.6847847
ACCGAGG46050.024.48456290-91
GCCCACG43100.024.00821382-83
CGCAGAG143950.024.0009218
CACGAGA45300.023.98301584-85
CCCACGA44600.023.57098882-83
ACGAGAC45550.023.46527986-87
TCCGAGC43750.023.18913576-77
TCTCCGA44300.022.86414574-75
Produced by FastQC (version 0.11.5)