##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4609030
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.145199098291833	18.0	18.0	30.0	18.0	32.0
2	28.573706181127047	29.0	27.0	32.0	25.0	33.0
3	30.503132546327535	31.0	29.0	33.0	27.0	33.0
4	31.42631594934292	33.0	31.0	33.0	29.0	33.0
5	32.630310065241495	33.0	33.0	33.0	32.0	34.0
6	36.617765994146275	38.0	37.0	38.0	34.0	38.0
7	37.04541237527202	38.0	38.0	38.0	35.0	38.0
8	37.23966691473043	38.0	38.0	38.0	36.0	38.0
9	37.40334690813469	38.0	38.0	38.0	37.0	38.0
10-11	37.454851888575256	38.0	38.0	38.0	37.0	38.0
12-13	37.496383946296724	38.0	38.0	38.0	37.0	38.0
14-15	37.49693623170168	38.0	38.0	38.0	37.0	38.0
16-17	37.51598340648683	38.0	38.0	38.0	37.0	38.0
18-19	37.51775818339217	38.0	38.0	38.0	37.0	38.0
20-21	37.520442656110276	38.0	38.0	38.0	37.0	38.0
22-23	37.530283051140614	38.0	38.0	38.0	37.5	38.0
24-25	37.535054109425076	38.0	38.0	38.0	38.0	38.0
26-27	37.50216785742046	38.0	38.0	38.0	37.5	38.0
28-29	37.47903848410817	38.0	38.0	38.0	37.0	38.0
30-31	37.47768606286081	38.0	38.0	38.0	37.0	38.0
32-33	37.467888495274366	38.0	38.0	38.0	37.0	38.0
34-35	37.44213684884958	38.0	38.0	38.0	37.0	38.0
36-37	37.42263093575876	38.0	38.0	38.0	37.0	38.0
38-39	37.413114920271035	38.0	38.0	38.0	37.0	38.0
40-41	37.404915927244474	38.0	38.0	38.0	37.0	38.0
42-43	37.36925455532514	38.0	38.0	38.0	37.0	38.0
44-45	37.32872693637539	38.0	38.0	38.0	37.0	38.0
46-47	37.30164624904396	38.0	38.0	38.0	37.0	38.0
48-49	37.26172648654811	38.0	38.0	38.0	37.0	38.0
50-51	37.22777151897816	38.0	38.0	38.0	37.0	38.0
52-53	37.19009159258589	38.0	38.0	38.0	37.0	38.0
54-55	37.170695848959625	38.0	38.0	38.0	36.5	38.0
56-57	37.12261688922301	38.0	38.0	38.0	36.0	38.0
58-59	37.091620948511434	38.0	38.0	38.0	36.0	38.0
60-61	37.116238881905545	38.0	38.0	38.0	36.0	38.0
62-63	37.127392514400256	38.0	38.0	38.0	36.0	38.0
64-65	37.13220403383093	38.0	38.0	38.0	36.0	38.0
66-67	37.12322472874568	38.0	38.0	38.0	36.0	38.0
68-69	37.08399924540787	38.0	38.0	38.0	36.0	38.0
70-71	37.07475569614836	38.0	38.0	38.0	36.0	38.0
72-73	37.08256046636458	38.0	38.0	38.0	36.0	38.0
74-75	37.091610925656795	38.0	38.0	38.0	36.0	38.0
76-77	37.07079918502729	38.0	38.0	38.0	36.0	38.0
78-79	37.027504101013065	38.0	38.0	38.0	36.0	38.0
80-81	36.96963436830276	38.0	38.0	38.0	36.0	38.0
82-83	36.9211792534646	38.0	38.0	38.0	35.5	38.0
84-85	36.89681177961363	38.0	38.0	38.0	35.0	38.0
86-87	36.916211654577566	38.0	38.0	38.0	35.0	38.0
88-89	36.865503884811865	38.0	38.0	38.0	35.0	38.0
90-91	36.87874290951818	38.0	38.0	38.0	35.0	38.0
92-93	36.86437206671769	38.0	38.0	38.0	35.0	38.0
94-95	36.86697518003993	38.0	38.0	38.0	35.0	38.0
96-97	36.85521253497906	38.0	38.0	38.0	35.0	38.0
98-99	36.83241262751184	38.0	38.0	38.0	35.0	38.0
100	35.281976248990574	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	20.0
21	47.0
22	178.0
23	585.0
24	1540.0
25	3261.0
26	6058.0
27	10221.0
28	16435.0
29	24262.0
30	32344.0
31	42662.0
32	55544.0
33	77413.0
34	121370.0
35	239056.0
36	893547.0
37	3084478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.40936813168931	27.438701852667478	12.858584127245862	22.293345888397344
2	14.618815671821364	21.184289122976942	41.11315574724652	23.083739457955172
3	17.943303819486257	26.800755821786957	30.689974860181863	24.56596549854492
4	12.827245464419413	17.784414066212165	38.98496898026948	30.40337148909894
5	13.33757862283387	37.683243545822	35.527648984710446	13.451528846633673
6	29.77016856041293	38.43244674042043	18.66659579130533	13.130788907861307
7	26.067437183094928	32.72259021963407	24.091468269896268	17.11850432737474
8	23.948965400528962	37.50368298752666	21.48456399719681	17.06278761474757
9	25.89080565758956	16.499523760964887	21.543773852632768	36.06589672881279
10-11	24.272222137846793	26.804056384966035	29.214823943432783	19.708897533754392
12-13	24.903190042156375	24.538482066725535	29.28691069487506	21.27141719624303
14-15	22.232389461556988	25.684753624949288	26.70237555407537	25.38048135941836
16-17	21.81622814344884	28.645691175800547	27.4799903667366	22.058090314014013
18-19	21.84429261688468	27.941486603471883	29.022581757983783	21.191639021659654
20-21	22.690640343961853	27.232406946453345	28.73507580115815	21.341876908426656
22-23	22.427216390430267	27.05724129124954	28.650359973221285	21.86518234509891
24-25	22.14462401055409	27.225363664768782	28.76202089987502	21.86799142480211
26-27	22.06973005606164	27.344014138453197	28.733847611466835	21.852408194018327
28-29	22.05021904661941	27.352698325897904	28.783434581090233	21.813648046392448
30-31	22.27090370130171	27.290517442417713	28.64129989474281	21.797278961537767
32-33	22.073567665070144	27.516574392435682	28.557031618857714	21.85282632363646
34-35	22.217261950296837	27.42303030091086	28.57651026983928	21.783197478953024
36-37	22.243265230838144	27.223047474125334	28.57681924128792	21.9568680537486
38-39	22.013037882850483	27.524749695726737	28.6773408041833	21.784871617239485
40-41	22.25616524342004	27.35256532865675	28.61282384882019	21.77844557910302
42-43	22.143442826574507	27.509233350668588	28.324917491175448	22.02240633158145
44-45	22.197181492703976	27.57473722692798	28.2526919789682	21.975389301399844
46-47	22.316063613275734	27.50471332992761	28.06350452415702	22.11571853263964
48-49	22.214357812491166	27.630347976479687	28.069862724324597	22.08543148670455
50-51	22.10557713404276	27.76271379503001	27.96729175239277	22.16441731853446
52-53	22.367575721771303	27.664441145926684	27.78425188231229	22.183731249989723
54-55	22.384226643237607	27.545476701561565	27.8700539801573	22.200242675043526
56-57	22.30435753474045	27.646894422114542	27.883677462481458	22.16507058066355
58-59	22.186640180683483	27.590305908528684	27.832288416168478	22.39076549461935
60-61	22.420779806995768	27.577800508902143	28.054146994334612	21.947272689767473
62-63	22.17411060097411	27.6060058821	28.044327321334002	22.17555619559189
64-65	22.10013801725395	27.56583895480408	28.03922119135643	22.294801836585542
66-67	22.145499103082926	27.797732174827424	27.98489965736356	22.071869064726087
68-69	22.139735249829126	27.84862475654224	27.927849976334752	22.083790017293886
70-71	22.245935155397927	27.539901073418687	27.713559804691734	22.500603966491646
72-73	22.291659830702336	27.6940007921537	27.572025657925504	22.44231371921846
74-75	22.064032411215997	27.83521774784552	27.824866021509138	22.275883819429342
76-77	22.26843654546856	27.378812450706185	27.833912453134584	22.518838550690667
78-79	22.24742000179715	27.4293266816536	27.440128273239413	22.883125043309843
80-81	22.531183176976015	27.729649585197265	27.372075462315326	22.367091775511398
82-83	22.636064465055846	27.3613295845951	27.46124486726958	22.541361083079476
84-85	22.419456461222982	27.372762199191502	27.416983944200652	22.790797395384864
86-87	22.244902583071656	27.703643533537754	27.518163402652725	22.533290480737868
88-89	22.649432221664647	27.68857993007387	27.476729734908467	22.185258113353015
90-91	22.710089209030652	27.65745073611889	27.498749262132733	22.133710792717732
92-93	22.53144135065638	27.414703569154714	27.374551776216027	22.67930330397288
94-95	22.603315075047277	27.643416599606564	27.46090235524685	22.292365970099308
96-97	22.95369321598524	27.487274624581232	27.58263640698943	21.976395752444102
98-99	23.62401255441824	29.577678184485045	27.890991508387053	18.907317752709663
100	30.168404361994334	40.600929819698955	29.23066581830671	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1074.0
1	1051.0
2	1309.5
3	1731.5
4	2165.0
5	2874.5
6	3480.5
7	4231.5
8	4985.0
9	5246.5
10	5224.5
11	5143.5
12	5510.0
13	6850.5
14	9771.0
15	13169.0
16	15346.0
17	16177.5
18	15971.5
19	14975.5
20	13970.0
21	13875.5
22	15268.5
23	18293.0
24	23336.5
25	30947.5
26	41223.5
27	51960.5
28	61220.0
29	71070.0
30	80369.0
31	88773.0
32	100310.5
33	113881.0
34	125405.5
35	137269.0
36	150941.0
37	160126.0
38	166389.5
39	171374.5
40	176397.5
41	180461.0
42	181711.0
43	184987.0
44	188809.0
45	190646.5
46	189721.0
47	188993.0
48	189193.5
49	188755.0
50	185994.0
51	180923.5
52	175404.5
53	168828.5
54	159834.5
55	148591.0
56	135898.0
57	122153.0
58	108293.5
59	93655.0
60	79342.0
61	64699.0
62	50625.5
63	39151.0
64	29420.5
65	21579.5
66	15686.5
67	11717.5
68	8836.0
69	6455.5
70	4639.5
71	3317.0
72	2305.5
73	1507.5
74	948.0
75	599.0
76	405.0
77	257.5
78	152.0
79	90.0
80	58.5
81	39.0
82	30.5
83	23.0
84	15.5
85	9.0
86	6.5
87	5.0
88	2.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004838328238262715
3	1.5187577429524217E-4
4	6.075030971809687E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.254755224505964E-5
26-27	4.8826381957501735E-4
28-29	0.0010311545435816925
30-31	5.976781616115229E-4
32-33	5.882624644101209E-4
34-35	8.305502614047664E-4
36-37	0.0
38-39	1.6578610299096918E-4
40-41	6.123288972145827E-4
42-43	2.0187662265345064E-4
44-45	0.0
46-47	0.0
48-49	0.0014305714267584923
50-51	0.003202797404779086
52-53	0.0
54-55	0.003919690166847355
56-57	0.040172898803249105
58-59	0.058780787987580796
60-61	0.03078674321381575
62-63	0.0068967257580089605
64-65	0.0
66-67	0.0
68-69	2.5263137684226693E-5
70-71	0.00309159962558429
72-73	0.007672915744151404
74-75	0.005456223854506267
76-77	0.006756820957969407
78-79	0.010012833782820568
80-81	0.011647065277342026
82-83	0.04501274016423087
84-85	0.02677209191954378
86-87	0.0012027672597801063
88-89	0.001344388642095299
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	16.0
20-21	82.0
22-23	229.0
24-25	365.0
26-27	1171.0
28-29	3410.0
30-31	10955.0
32-33	13454.0
34-35	18289.0
36-37	29114.0
38-39	32940.0
40-41	32422.0
42-43	35847.0
44-45	42620.0
46-47	43620.0
48-49	41692.0
50-51	37275.0
52-53	34198.0
54-55	37209.0
56-57	39954.0
58-59	21726.0
60-61	41070.0
62-63	38805.0
64-65	40288.0
66-67	42876.0
68-69	44421.0
70-71	44061.0
72-73	40387.0
74-75	41186.0
76-77	42997.0
78-79	44626.0
80-81	44495.0
82-83	41447.0
84-85	40598.0
86-87	41212.0
88-89	43762.0
90-91	44942.0
92-93	43454.0
94-95	48827.0
96-97	159144.0
98-99	1031810.0
100-101	2172034.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.49471461274955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.06410291411447	81.47186513463365
2	5.751473283064554	10.179499735753028
3	1.2676346816004815	3.3653690814437476
4	0.4313861043452925	1.5270156076776984
5	0.19053284521346764	0.8430574880760502
6	0.09586998736686587	0.509039230317522
7	0.058681195777204896	0.36350829714070426
8	0.03746666259778068	0.26524812912662227
9	0.022967044568876053	0.18292158491588872
>10	0.07773556352949512	1.0533260655519023
>50	0.00122137897921879	0.07044168973988642
>100	8.577992946639829E-4	0.1269776644646418
>500	7.053954763487404E-5	0.041730291158637335
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1696539185034596E-5	0.0	0.0	0.0
3	0.0	2.1696539185034596E-5	0.0	0.0	0.0
4	0.0	6.508961755510379E-5	0.0	0.0	0.0
5	0.0	6.508961755510379E-5	0.0	0.0	0.0
6	0.0	6.508961755510379E-5	0.0	0.0	0.0
7	0.0	6.508961755510379E-5	0.0	0.0	0.0
8	2.1696539185034596E-5	6.508961755510379E-5	0.0	0.0	0.0
9	2.1696539185034596E-5	1.3017923511020758E-4	0.0	2.1696539185034596E-5	0.0
10-11	2.1696539185034596E-5	1.3017923511020758E-4	0.0	3.2544808777551894E-5	1.0848269592517298E-5
12-13	2.1696539185034596E-5	1.3017923511020758E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
14-15	2.1696539185034596E-5	2.9290327899796705E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
16-17	2.1696539185034596E-5	3.0375154859048435E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
18-19	2.1696539185034596E-5	3.2544808777551894E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
20-21	2.1696539185034596E-5	3.6884116614558813E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
22-23	2.1696539185034596E-5	4.122342445156573E-4	0.0	4.339307837006919E-5	2.1696539185034596E-5
24-25	2.1696539185034596E-5	4.339307837006919E-4	0.0	6.508961755510379E-5	8.678615674013838E-5
26-27	2.1696539185034596E-5	4.556273228857265E-4	0.0	1.3017923511020758E-4	8.678615674013838E-5
28-29	2.1696539185034596E-5	4.556273228857265E-4	0.0	5.966548275884514E-4	8.678615674013838E-5
30-31	2.1696539185034596E-5	4.773238620707611E-4	0.0	0.001822509291542906	8.678615674013838E-5
32-33	2.1696539185034596E-5	4.773238620707611E-4	0.0	0.005228865943593337	1.3017923511020758E-4
34-35	2.1696539185034596E-5	5.532617492183822E-4	0.0	0.008700312213198873	1.5187577429524217E-4
36-37	2.1696539185034596E-5	6.291996363660033E-4	0.0	0.015513025517299736	1.5187577429524217E-4
38-39	2.1696539185034596E-5	6.400479059585206E-4	0.0	0.03510500040138598	1.6272404388775947E-4
40-41	2.1696539185034596E-5	7.376823322911763E-4	0.0	0.05600961590616681	1.9526885266531136E-4
42-43	2.1696539185034596E-5	7.702271410687282E-4	0.0	0.06702060954257186	2.0611712225782866E-4
44-45	2.1696539185034596E-5	8.570132978088665E-4	0.0	0.08099318077773415	2.1696539185034596E-4
46-47	2.1696539185034596E-5	9.11254645771453E-4	0.0	0.09598548935459306	2.1696539185034596E-4
48-49	2.1696539185034596E-5	9.221029153639703E-4	0.0	0.11042453618223358	2.1696539185034596E-4
50-51	4.339307837006919E-5	0.0010305856112891433	0.0	0.12384384566817747	2.1696539185034596E-4
52-53	4.339307837006919E-5	0.0010414338808816606	0.0	0.16659687613228813	2.1696539185034596E-4
54-55	4.339307837006919E-5	0.0010414338808816606	0.0	0.2230838159005257	2.1696539185034596E-4
56-57	4.339307837006919E-5	0.0010631304200666952	0.0	0.29449797462806704	2.1696539185034596E-4
58-59	4.339307837006919E-5	0.001095675228844247	0.0	0.5491285585036331	2.1696539185034596E-4
60-61	4.339307837006919E-5	0.001128220037621799	0.0	0.7740999733132568	2.1696539185034596E-4
62-63	4.339307837006919E-5	0.0011499165768068336	0.0	0.7829196164919734	2.1696539185034596E-4
64-65	4.339307837006919E-5	0.0012150061943619374	0.0	0.7909256394512512	2.1696539185034596E-4
66-67	4.339307837006919E-5	0.0012150061943619374	0.0	0.7982265248870153	2.1696539185034596E-4
68-69	4.339307837006919E-5	0.0012150061943619374	0.0	0.806568844203661	2.1696539185034596E-4
70-71	6.508961755510379E-5	0.0012150061943619374	0.0	0.812774054410581	2.3866193103538056E-4
72-73	1.0848269592517298E-4	0.0012150061943619374	0.0	0.8173954172569933	2.3866193103538056E-4
74-75	1.0848269592517298E-4	0.0012583992727320066	0.0	0.8212031598839669	2.3866193103538056E-4
76-77	1.0848269592517298E-4	0.0013234888902871104	0.0	0.8253255023291235	2.3866193103538056E-4
78-79	1.0848269592517298E-4	0.0013777302382496969	0.0	0.8278531491441801	2.3866193103538056E-4
80-81	1.0848269592517298E-4	0.0013885785078422141	0.0	0.8285365901285087	2.4951020062789785E-4
82-83	1.0848269592517298E-4	0.0013994267774347314	0.0	0.8291440932256895	2.8205500940544975E-4
84-85	1.0848269592517298E-4	0.0014102750470272487	0.0	0.8294478447742801	2.8205500940544975E-4
86-87	1.0848269592517298E-4	0.0014319715862122833	0.0	0.8296105688181679	2.8205500940544975E-4
88	1.0848269592517298E-4	0.0014970612037673871	0.0	0.8296322653573528	2.8205500940544975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4645	0.0	50.797485	1
GTATCAA	9100	0.0	35.51353	1
TCAACGC	11260	0.0	30.683239	4
CAACGCA	11490	0.0	30.03237	5
ATCAACG	11505	0.0	30.029837	3
AACGCAG	11810	0.0	29.147272	6
TATCAAC	12125	0.0	28.46047	2
CGAGACC	4020	0.0	27.219526	86-87
AGACCGA	4245	0.0	26.37171	88-89
GAGACCG	4230	0.0	25.047447	88-89
GACCGAG	4340	0.0	24.841305	90-91
ACGCAGA	13945	0.0	24.684784	7
ACCGAGG	4605	0.0	24.484562	90-91
GCCCACG	4310	0.0	24.008213	82-83
CGCAGAG	14395	0.0	24.000921	8
CACGAGA	4530	0.0	23.983015	84-85
CCCACGA	4460	0.0	23.570988	82-83
ACGAGAC	4555	0.0	23.465279	86-87
TCCGAGC	4375	0.0	23.189135	76-77
TCTCCGA	4430	0.0	22.864145	74-75
>>END_MODULE