FastQCFastQC Report
Fri 27 May 2016
SRR2139176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5211744
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT61100.063.10123494
GGTATCA71550.054.4686051
TATCTCG73050.044.2099494
GTATCAA135900.040.0853651
GCCGTCT33800.035.7575290-91
CCGTCTT32950.035.10794490-91
TGCCGTC33550.034.04866488-89
TATGCCG32850.033.98786586-87
ATGCCGT35200.033.33624688-89
TCTCGTA33850.032.61083282-83
TCAACGC167350.032.220044
ATCAACG168950.031.8395883
TATCAAC172450.031.3918652
CAACGCA172100.031.3060135
AACGCAG177450.030.4101626
CTCGTAT36050.029.86650782-83
TCGTATG40400.028.71247984-85
CTATCTC93650.026.90171292-93
CGTATGC41900.026.82971884-85
ACGCAGA206150.026.1351687
Produced by FastQC (version 0.11.5)