##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5211744
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.981519046215624	18.0	18.0	32.0	18.0	33.0
2	27.852820859965494	29.0	25.0	31.0	18.0	33.0
3	30.5248638459602	31.0	29.0	33.0	27.0	33.0
4	31.430764250891833	33.0	31.0	33.0	29.0	33.0
5	32.45787321863852	33.0	33.0	33.0	31.0	34.0
6	36.23220115953508	38.0	36.0	38.0	33.0	38.0
7	36.84454397606636	38.0	37.0	38.0	35.0	38.0
8	36.97704933319825	38.0	38.0	38.0	35.0	38.0
9	37.24460833072384	38.0	38.0	38.0	36.0	38.0
10-11	37.34208117282814	38.0	38.0	38.0	37.0	38.0
12-13	37.37208849859088	38.0	38.0	38.0	37.0	38.0
14-15	37.31466923548048	38.0	38.0	38.0	37.0	38.0
16-17	37.391803684141045	38.0	38.0	38.0	37.0	38.0
18-19	37.40668948820203	38.0	38.0	38.0	37.0	38.0
20-21	37.415280554084454	38.0	38.0	38.0	37.0	38.0
22-23	37.42449847989659	38.0	38.0	38.0	37.0	38.0
24-25	37.43018363373949	38.0	38.0	38.0	37.0	38.0
26-27	37.39827541470608	38.0	38.0	38.0	37.0	38.0
28-29	37.37726193806732	38.0	38.0	38.0	37.0	38.0
30-31	37.375321441309325	38.0	38.0	38.0	37.0	38.0
32-33	37.355418835329786	38.0	38.0	38.0	37.0	38.0
34-35	37.33350523190986	38.0	38.0	38.0	37.0	38.0
36-37	37.31192059000089	38.0	38.0	38.0	37.0	38.0
38-39	37.30655791740105	38.0	38.0	38.0	37.0	38.0
40-41	37.29273971469796	38.0	38.0	38.0	37.0	38.0
42-43	37.24507659184633	38.0	38.0	38.0	37.0	38.0
44-45	37.14881214708505	38.0	38.0	38.0	36.0	38.0
46-47	37.16428885162612	38.0	38.0	38.0	36.5	38.0
48-49	37.11507105451001	38.0	38.0	38.0	36.0	38.0
50-51	37.062438144905414	38.0	38.0	38.0	36.0	38.0
52-53	37.03666387204971	38.0	38.0	38.0	36.0	38.0
54-55	37.00606588483873	38.0	38.0	38.0	36.0	38.0
56-57	36.95944935696107	38.0	38.0	38.0	36.0	38.0
58-59	36.9451616206662	38.0	38.0	38.0	36.0	38.0
60-61	36.95244822720735	38.0	38.0	38.0	36.0	38.0
62-63	36.957410437120146	38.0	38.0	38.0	36.0	38.0
64-65	36.91821468177352	38.0	38.0	38.0	36.0	38.0
66-67	36.94442106884521	38.0	38.0	38.0	36.0	38.0
68-69	36.926685406448264	38.0	38.0	38.0	35.5	38.0
70-71	36.93311847443931	38.0	38.0	38.0	35.5	38.0
72-73	36.91838225927275	38.0	38.0	38.0	35.0	38.0
74-75	36.89747222720602	38.0	38.0	38.0	35.0	38.0
76-77	36.87860255104827	38.0	38.0	38.0	35.0	38.0
78-79	36.85108640132343	38.0	38.0	38.0	35.0	38.0
80-81	36.80465958277074	38.0	38.0	38.0	35.0	38.0
82-83	36.737115571741825	38.0	38.0	38.0	35.0	38.0
84-85	36.7486164143289	38.0	38.0	38.0	35.0	38.0
86-87	36.74443999359974	38.0	38.0	38.0	35.0	38.0
88-89	36.71831175592973	38.0	38.0	38.0	34.5	38.0
90-91	36.70106819331959	38.0	38.0	38.0	34.0	38.0
92-93	36.675347069512455	38.0	38.0	38.0	34.0	38.0
94-95	36.59710922899823	38.0	38.0	38.0	34.0	38.0
96-97	36.53248911537504	38.0	38.0	38.0	34.0	38.0
98-99	36.57317124044424	38.0	38.0	38.0	34.0	38.0
100	35.10112525995395	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	5.0
20	14.0
21	47.0
22	265.0
23	840.0
24	2270.0
25	4751.0
26	8867.0
27	15152.0
28	24673.0
29	35365.0
30	46721.0
31	59567.0
32	77478.0
33	107096.0
34	164751.0
35	315909.0
36	1062854.0
37	3285112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10144320212198	21.7692964197781	13.12201827257824	24.007242105521684
2	14.880183956884338	20.590134882271585	40.81971258271843	23.709968578125647
3	18.194335606960106	26.420741708374557	30.750814906006628	24.634107778658713
4	12.986516605573872	17.825990685651483	38.700634566855165	30.48685814191948
5	13.604543891641645	37.365687953974714	35.11592280818091	13.91384534620273
6	29.776816440026792	38.139930414513906	18.672188581157194	13.411064564302105
7	26.32402896228211	32.77996770370916	23.71511340541669	17.18088992859204
8	24.27979962177728	36.938191898911384	21.271842976170742	17.5101655031406
9	26.312132752491298	16.247689832808366	21.257164588283693	36.18301282641664
10-11	24.49632023368761	26.680138164883004	28.795831107590857	20.02771049383853
12-13	25.296676122234707	24.52147496116463	28.93792365856803	21.24392525803263
14-15	22.64170112730019	25.444313841969212	26.612732705213453	25.301252325517137
16-17	21.926307201581658	28.447281754437668	27.35580450613077	22.2706065378499
18-19	21.96854834005661	27.58589063468965	29.176241196804753	21.269319828448978
20-21	22.923459495345554	26.7605198702531	28.95090551328409	21.36511512111726
22-23	22.656681586849416	26.632823621718778	28.787170855888384	21.92332393554343
24-25	22.30486609964779	26.81615677569134	29.006676883292133	21.872300241368738
26-27	22.142462934434853	26.975359485384654	28.96795659257344	21.91422098760705
28-29	22.076897192475315	27.00348450453936	28.99666526339235	21.922953039592976
30-31	22.425638463270207	26.879630984292458	28.818009298448356	21.876721253988983
32-33	22.195499306795412	27.06508567576991	28.752436046021923	21.986978971412753
34-35	22.334067094740888	27.021003134705307	28.855851416070564	21.78907835448324
36-37	22.398616067954663	26.846121107266104	28.791238490750874	21.964024334028363
38-39	22.124576559448283	27.107568622345863	28.756749144573668	22.011105673632184
40-41	22.421202973860517	26.836815564720457	28.874009848019323	21.867971613399707
42-43	22.220977860427165	26.978839743199373	28.521336761434558	22.278845634938907
44-45	22.14562575714054	27.097590888086824	28.49324224750534	22.2635411072673
46-47	22.339464850015727	27.01665773858059	28.42035482452398	22.223522586879703
48-49	22.27161767693563	27.399807368270785	28.10440602988945	22.224168924904138
50-51	22.195115766453334	27.461567862965374	27.813852144505823	22.52946422607547
52-53	22.259809680408615	27.22217782261162	27.72350858749928	22.794503909480486
54-55	22.49696678225625	26.988788217353672	28.076887180855103	22.437357819534977
56-57	22.21888547617691	26.665658391559493	28.22487297287498	22.890583159388612
58-59	21.993701329396835	26.384681251606807	28.3283643360065	23.293253082989857
60-61	22.305390535192483	26.770081052542288	28.645619832362435	22.278908579902797
62-63	21.7775562097089	26.96569915276591	28.711033034743984	22.545711602781207
64-65	21.4828063559499	27.36189379416044	28.302193179483726	22.85310667040593
66-67	21.595307006016657	28.214177929840023	27.66170631598702	22.5288087481563
68-69	21.725651411406808	28.11400970896833	27.491862189359072	22.66847669026579
70-71	21.771403262519804	27.444499042574055	27.41671275679177	23.367384938114366
72-73	22.0667041755702	26.922907165909578	27.362815717895	23.64757294062522
74-75	22.066954514175087	26.9971617833086	27.607876344243508	23.328007358272806
76-77	22.473248181095055	26.75806211630738	26.96504507985956	23.803644622738005
78-79	22.73582908809694	26.82796105526885	26.351158791593672	24.085051065040542
80-81	22.54054420718733	27.38623471817271	26.498078202185905	23.575142872454062
82-83	22.58472539471966	26.953715654232578	26.62213316099339	23.83942579005437
84-85	22.54873890086912	27.54635249275852	26.49459800719069	23.41031059918167
86-87	23.2077473691141	27.43427823057878	26.550177603179463	22.807796797127654
88-89	22.903719325829922	27.61591831853497	26.936489249704458	22.54387310593065
90-91	22.922067893954615	27.068124859408776	26.795644787982226	23.214162458654386
92-93	22.901035432572584	26.535592224102928	27.000770705443323	23.562601637881166
94-95	21.975225918614967	27.081322029134185	27.838295095916415	23.105156956334433
96-97	22.150544141081923	26.89981903517283	28.026460132679837	22.923176691065407
98-99	23.344955306911856	28.768673961599767	28.303293173420883	19.583077558067497
100	30.35042734343222	40.05197851456519	29.59759414200259	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1948.0
1	1743.5
2	1899.5
3	2399.0
4	2999.0
5	4264.0
6	5413.0
7	6382.5
8	7366.5
9	7860.0
10	7493.5
11	7069.5
12	7798.5
13	9722.0
14	13612.0
15	18355.0
16	21896.0
17	23740.0
18	23399.5
19	21233.0
20	18466.0
21	16107.0
22	15214.0
23	16627.5
24	20403.5
25	26286.0
26	34726.0
27	44646.5
28	54408.5
29	65752.5
30	76702.5
31	86718.0
32	99913.0
33	115696.5
34	129096.0
35	141619.5
36	156607.5
37	168994.5
38	177841.0
39	183511.0
40	189547.5
41	195810.5
42	199472.5
43	204716.0
44	211282.0
45	216215.0
46	217857.5
47	220482.5
48	223469.0
49	222555.5
50	219687.0
51	216164.5
52	208768.5
53	198645.0
54	187436.5
55	173859.0
56	158808.0
57	143266.0
58	127457.0
59	109087.0
60	90528.5
61	72803.5
62	56763.5
63	44067.5
64	33210.5
65	24286.0
66	17545.0
67	12874.5
68	9442.0
69	6963.5
70	5047.0
71	3448.5
72	2345.0
73	1529.5
74	973.5
75	616.0
76	398.0
77	250.5
78	165.0
79	105.0
80	65.5
81	41.0
82	26.5
83	19.5
84	13.5
85	9.0
86	7.0
87	4.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00168849429288929
3	0.0019187435146469207
4	0.0
5	0.0
6	4.7968587866173017E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.2066496716265435E-4
24-25	9.882626065128809E-4
26-27	0.002840468266547623
28-29	0.001843263644207107
30-31	0.0029702013825374267
32-33	0.003411456325649526
34-35	0.0011602364910039615
36-37	9.714802541780936E-6
38-39	8.789371501341843E-5
40-41	1.965122997048385E-5
42-43	6.040234297717802E-4
44-45	0.0
46-47	0.0
48-49	2.653041462038089E-4
50-51	0.006225610346881938
52-53	0.0
54-55	0.0030315737892820313
56-57	0.04870495956059512
58-59	0.0608866829107129
60-61	0.03622588704603424
62-63	0.00828173950289518
64-65	0.0
66-67	0.0
68-69	6.533290271691231E-4
70-71	0.00251194053135034
72-73	0.0102641108399152
74-75	0.0050583313477411285
76-77	0.003543010841499252
78-79	0.0038504237195323694
80-81	0.01629593606670034
82-83	0.05504024936448372
84-85	0.03554717350333781
86-87	0.01029845927304253
88-89	0.008931642063402898
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	33.0
20-21	124.0
22-23	327.0
24-25	572.0
26-27	1644.0
28-29	4343.0
30-31	12683.0
32-33	15889.0
34-35	21673.0
36-37	33699.0
38-39	22080.0
40-41	39287.0
42-43	42445.0
44-45	51837.0
46-47	52488.0
48-49	49965.0
50-51	25558.0
52-53	3721.0
54-55	3546.0
56-57	2923.0
58-59	26133.0
60-61	49789.0
62-63	46596.0
64-65	47871.0
66-67	51261.0
68-69	53497.0
70-71	52968.0
72-73	48665.0
74-75	48678.0
76-77	50890.0
78-79	53547.0
80-81	54502.0
82-83	50060.0
84-85	48591.0
86-87	49739.0
88-89	52925.0
90-91	53862.0
92-93	52908.0
94-95	59614.0
96-97	182440.0
98-99	1248669.0
100-101	2443702.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.38487349314943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.16206232894595	76.98476285838504
2	6.893790377715716	11.772508385790942
3	1.6413015180887933	4.204269674583772
4	0.604987658797911	2.066271788455052
5	0.26879184554949953	1.147537886411709
6	0.144464009251849	0.7401024692569378
7	0.0775903650388112	0.4637530452188325
8	0.053813459746274994	0.3675884362131504
9	0.03275327400511751	0.2516970741672076
>10	0.1169702097056791	1.560110977614681
>50	0.0019777926447137064	0.10888459940057307
>100	0.0013910636465169876	0.26363369670493053
>500	7.07312423792366E-5	0.037232766467200674
>1k	3.536562080220559E-5	0.03164634132996763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.837487029293841E-5	0.0	0.0	0.0
5	0.0	5.7562305439407614E-5	0.0	0.0	0.0
6	7.674974058587682E-5	5.7562305439407614E-5	0.0	1.9187435146469206E-5	0.0
7	7.674974058587682E-5	5.7562305439407614E-5	0.0	1.9187435146469206E-5	0.0
8	7.674974058587682E-5	7.674974058587682E-5	0.0	1.9187435146469206E-5	0.0
9	7.674974058587682E-5	9.593717573234602E-5	0.0	3.837487029293841E-5	0.0
10-11	7.674974058587682E-5	9.593717573234602E-5	0.0	3.837487029293841E-5	0.0
12-13	7.674974058587682E-5	9.593717573234602E-5	0.0	3.837487029293841E-5	0.0
14-15	7.674974058587682E-5	1.1512461087881523E-4	0.0	3.837487029293841E-5	0.0
16-17	7.674974058587682E-5	1.1512461087881523E-4	0.0	4.7968587866173017E-5	0.0
18-19	7.674974058587682E-5	1.3431204602528442E-4	0.0	5.7562305439407614E-5	0.0
20-21	8.634345815911142E-5	1.5349948117175365E-4	0.0	7.674974058587681E-5	0.0
22-23	9.593717573234602E-5	1.5349948117175365E-4	0.0	1.0553089330558063E-4	0.0
24-25	9.593717573234602E-5	1.7268691631822284E-4	0.0	1.3431204602528442E-4	0.0
26-27	9.593717573234602E-5	1.9187435146469204E-4	0.0	2.0146806903792664E-4	0.0
28-29	9.593717573234602E-5	1.9187435146469204E-4	0.0	7.387162531390643E-4	0.0
30-31	9.593717573234602E-5	2.3024922175763046E-4	0.0	0.0025231477217607006	0.0
32-33	9.593717573234602E-5	2.3024922175763046E-4	0.0	0.006869101782435976	0.0
34-35	9.593717573234602E-5	3.3578011506321106E-4	0.0	0.012184021318007946	3.837487029293841E-5
36-37	9.593717573234602E-5	4.221235732223225E-4	0.0	0.024339261483296185	3.837487029293841E-5
38-39	9.593717573234602E-5	4.413110083687917E-4	0.0	0.22425314827435883	3.837487029293841E-5
40-41	9.593717573234602E-5	4.988733138081993E-4	0.0	0.4343651568457698	3.837487029293841E-5
42-43	9.593717573234602E-5	5.08467031381434E-4	0.0	0.46481561642321645	3.837487029293841E-5
44-45	9.593717573234602E-5	5.56435619247607E-4	0.0	0.5041594521910516	3.837487029293841E-5
46-47	9.593717573234602E-5	5.948104895405453E-4	0.0	0.5507369510091056	3.837487029293841E-5
48-49	9.593717573234602E-5	5.948104895405453E-4	0.0	0.5971129817581217	3.837487029293841E-5
50-51	9.593717573234602E-5	6.52372794979953E-4	0.0	0.6441413853021176	3.837487029293841E-5
52-53	9.593717573234602E-5	6.715602301264221E-4	0.0	1.2607775823217717	4.7968587866173017E-5
54-55	9.593717573234602E-5	6.811539476996567E-4	0.0	2.0531610915655105	5.7562305439407614E-5
56-57	9.593717573234602E-5	6.907476652728914E-4	0.0	2.928223258855385	5.7562305439407614E-5
58-59	9.593717573234602E-5	6.907476652728914E-4	0.0	3.851935167959132	5.7562305439407614E-5
60-61	9.593717573234602E-5	7.00341382846126E-4	0.0	4.100757826938545	5.7562305439407614E-5
62-63	9.593717573234602E-5	7.195288179925952E-4	0.0	4.11070651206199	5.7562305439407614E-5
64-65	9.593717573234602E-5	8.250597112981758E-4	0.0	4.119571107099658	5.7562305439407614E-5
66-67	9.593717573234602E-5	8.250597112981758E-4	0.0	4.12811910945741	6.715602301264221E-5
68-69	9.593717573234602E-5	8.634345815911142E-4	0.0	4.135132117003444	7.674974058587682E-5
70-71	1.1512461087881523E-4	8.92215734310818E-4	0.0	4.140974691005544	7.674974058587682E-5
72-73	1.1512461087881523E-4	9.114031694572873E-4	0.0	4.145723581204296	9.593717573234602E-5
74-75	1.1512461087881523E-4	9.689654748966949E-4	0.0	4.149302037859112	9.593717573234602E-5
76-77	1.1512461087881523E-4	0.0010361214979093371	0.0	4.152909275666648	9.593717573234602E-5
78-79	1.1512461087881523E-4	0.0010744963682022754	0.0	4.155470798258702	9.593717573234602E-5
80-81	1.1512461087881523E-4	0.0010744963682022754	0.0	4.156286264252427	9.593717573234602E-5
82-83	1.1512461087881523E-4	0.0010744963682022754	0.0	4.156900262177114	9.593717573234602E-5
84-85	1.1512461087881523E-4	0.0010744963682022754	0.0	4.15727441716247	9.593717573234602E-5
86-87	1.1512461087881523E-4	0.001112871238495214	0.0	4.1574758852315075	9.593717573234602E-5
88	1.1512461087881523E-4	0.001132058673641683	0.0	4.1575142601018005	9.593717573234602E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	6110	0.0	63.101234	94
GGTATCA	7155	0.0	54.468605	1
TATCTCG	7305	0.0	44.20994	94
GTATCAA	13590	0.0	40.085365	1
GCCGTCT	3380	0.0	35.75752	90-91
CCGTCTT	3295	0.0	35.107944	90-91
TGCCGTC	3355	0.0	34.048664	88-89
TATGCCG	3285	0.0	33.987865	86-87
ATGCCGT	3520	0.0	33.336246	88-89
TCTCGTA	3385	0.0	32.610832	82-83
TCAACGC	16735	0.0	32.22004	4
ATCAACG	16895	0.0	31.839588	3
TATCAAC	17245	0.0	31.391865	2
CAACGCA	17210	0.0	31.306013	5
AACGCAG	17745	0.0	30.410162	6
CTCGTAT	3605	0.0	29.866507	82-83
TCGTATG	4040	0.0	28.712479	84-85
CTATCTC	9365	0.0	26.901712	92-93
CGTATGC	4190	0.0	26.829718	84-85
ACGCAGA	20615	0.0	26.135168	7
>>END_MODULE