FastQCFastQC Report
Fri 27 May 2016
SRR2139175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6121690
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74400.055.0634731
GTATCAA138500.038.1146771
TCAACGC168850.030.702894
ATCAACG172450.030.0125853
CAACGCA174600.029.6179125
TATCAAC179550.029.0893862
AACGCAG179150.028.9136736
ACGCAGA210100.024.6337157
CGCAGAG218800.023.6738668
GTACATG198250.022.6809981
TACATGG202550.021.7962532
ACATGGG198650.021.6829953
GTGGTAT36100.020.9579661
GCAGAGT254450.020.424599
TGGTATC33400.020.2067682
GAGTACT145950.019.37988912-13
CATGGGG137100.018.7811584
CAGAGTA247150.018.61026810-11
AGAGTAC231300.018.0735510-11
GTACTTT166900.017.01163114-15
Produced by FastQC (version 0.11.5)