##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6121690
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.90124867479405	28.0	18.0	32.0	18.0	33.0
2	27.768301400430275	29.0	25.0	31.0	18.0	33.0
3	30.62486715269803	31.0	29.0	33.0	27.0	33.0
4	30.10260793996429	31.0	29.0	33.0	25.0	33.0
5	31.711964996594077	33.0	31.0	33.0	29.0	33.0
6	36.073396071999724	38.0	36.0	38.0	33.0	38.0
7	36.411800172828094	38.0	37.0	38.0	34.0	38.0
8	36.63226984705204	38.0	37.0	38.0	34.0	38.0
9	36.93433349287533	38.0	38.0	38.0	35.0	38.0
10-11	37.1144992640921	38.0	38.0	38.0	36.0	38.0
12-13	37.218544797270035	38.0	38.0	38.0	36.0	38.0
14-15	37.13648494124988	38.0	38.0	38.0	36.0	38.0
16-17	37.33007551836176	38.0	38.0	38.0	36.5	38.0
18-19	37.36279736477999	38.0	38.0	38.0	37.0	38.0
20-21	37.40022431456579	38.0	38.0	38.0	37.0	38.0
22-23	37.421415790841564	38.0	38.0	38.0	37.0	38.0
24-25	37.438883378106496	38.0	38.0	38.0	37.0	38.0
26-27	37.40040711152784	38.0	38.0	38.0	37.0	38.0
28-29	37.37464607533599	38.0	38.0	38.0	37.0	38.0
30-31	37.383862693481646	38.0	38.0	38.0	37.0	38.0
32-33	37.370631894422274	38.0	38.0	38.0	37.0	38.0
34-35	37.341367185560955	38.0	38.0	38.0	37.0	38.0
36-37	37.31879482975315	38.0	38.0	38.0	37.0	38.0
38-39	37.32904157494123	38.0	38.0	38.0	37.0	38.0
40-41	37.323858312705894	38.0	38.0	38.0	37.0	38.0
42-43	37.28381959787866	38.0	38.0	38.0	37.0	38.0
44-45	37.19375611920192	38.0	38.0	38.0	37.0	38.0
46-47	37.22347599971831	38.0	38.0	38.0	37.0	38.0
48-49	37.1762154788032	38.0	38.0	38.0	37.0	38.0
50-51	37.13198016605892	38.0	38.0	38.0	36.0	38.0
52-53	37.11592662527243	38.0	38.0	38.0	36.0	38.0
54-55	37.09434651775348	38.0	38.0	38.0	36.0	38.0
56-57	37.060199508494605	38.0	38.0	38.0	36.0	38.0
58-59	37.05452328019007	38.0	38.0	38.0	36.0	38.0
60-61	37.065091403972744	38.0	38.0	38.0	36.0	38.0
62-63	37.079746050806946	38.0	38.0	38.0	36.0	38.0
64-65	37.043427501751864	38.0	38.0	38.0	36.0	38.0
66-67	37.078575355756456	38.0	38.0	38.0	36.0	38.0
68-69	37.074203354226086	38.0	38.0	38.0	36.0	38.0
70-71	37.08230953249928	38.0	38.0	38.0	36.0	38.0
72-73	37.07087933743439	38.0	38.0	38.0	36.0	38.0
74-75	37.066896946302556	38.0	38.0	38.0	36.0	38.0
76-77	37.06015007236651	38.0	38.0	38.0	36.0	38.0
78-79	37.041783857049225	38.0	38.0	38.0	36.0	38.0
80-81	37.01484105423711	38.0	38.0	38.0	36.0	38.0
82-83	36.984931297730824	38.0	38.0	38.0	36.0	38.0
84-85	36.96930820424465	38.0	38.0	38.0	36.0	38.0
86-87	36.96884404213811	38.0	38.0	38.0	36.0	38.0
88-89	36.95533267288396	38.0	38.0	38.0	36.0	38.0
90-91	36.93528311087978	38.0	38.0	38.0	35.0	38.0
92-93	36.91339261107714	38.0	38.0	38.0	35.0	38.0
94-95	36.890109748706756	38.0	38.0	38.0	35.0	38.0
96-97	36.89364518028687	38.0	38.0	38.0	35.0	38.0
98-99	36.90050024504544	38.0	38.0	38.0	35.0	38.0
100	35.55925345854078	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	9.0
20	19.0
21	90.0
22	367.0
23	1089.0
24	2784.0
25	5811.0
26	10432.0
27	17218.0
28	26894.0
29	39421.0
30	52546.0
31	68586.0
32	88921.0
33	122092.0
34	189031.0
35	364533.0
36	1157409.0
37	3974433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03043603972106	20.70343320226931	13.272691037932335	23.9934397200773
2	15.393799798254534	20.900487211216067	41.56969407382904	22.13601891670036
3	18.195884759679675	27.177712529106245	29.98308594996878	24.643316761245295
4	12.831017714228867	17.9162156065099	38.86595676454652	30.386809914714714
5	13.428497686096485	37.56292788429339	35.56468883592603	13.443885593684096
6	29.622462004599402	38.14972143185952	18.91079579369132	13.317020769849758
7	26.117085314676174	32.70337112790749	24.035225566796097	17.144317990620237
8	23.960050247562357	37.32549345033806	21.48767742241113	17.22677887968845
9	26.008210151118398	16.672275139708155	21.462373952290953	35.857140756882494
10-11	24.3000175278424	26.729102050104146	29.141761366838466	19.829119055214992
12-13	25.130584854835835	24.476427261099467	29.175652475051823	21.217335409012872
14-15	22.41675909756946	25.530784799622324	26.748912473516302	25.303543629291912
16-17	21.814507105064123	28.512673787793897	27.497267911312072	22.17555119582991
18-19	21.87745540855548	27.686431361274423	29.232769055603924	21.203344174566176
20-21	22.757576175863317	26.916150989265052	29.024164355943476	21.302108478928155
22-23	22.555091842539778	26.78334864144429	28.854578935739834	21.8069805802761
24-25	22.19921728766512	26.964105849055663	28.967438392504807	21.86923847077441
26-27	22.099681939153278	27.066950280588582	28.982177515106134	21.851190265152002
28-29	22.105244750987683	27.001114318887392	29.047630370693046	21.846010559431882
30-31	22.339086544798935	26.968698002681496	28.858665094010988	21.83355035850858
32-33	22.11877754335377	27.170804685870326	28.79305596061742	21.917361810158482
34-35	22.25293459891339	27.08606772690427	28.84185408255594	21.8191435916264
36-37	22.27127212677811	26.95224125398025	28.786663422766644	21.98982319647499
38-39	22.106363844007497	27.192278991045622	28.86617335003973	21.835183814907147
40-41	22.31853774968591	27.033241110682717	28.77057594835598	21.877645191275395
42-43	22.213190363104676	27.168424204840562	28.511024815752023	22.107360616302742
44-45	22.24952306855686	27.255830471660637	28.3952724435941	22.0993740161884
46-47	22.378910145372263	27.207991067145688	28.19115034716251	22.22194844031954
48-49	22.314954418244	27.322057863536546	28.126733893787815	22.236253824431635
50-51	22.304752807742087	27.459127592934575	27.996375205117275	22.239744394206067
52-53	22.501394409628418	27.353040297400238	27.865926995216796	22.279638297754552
54-55	22.499743379026523	27.27562729353635	27.874017777238368	22.350611550198764
56-57	22.407069753635938	27.397847843883554	27.91637468557485	22.27870771690566
58-59	22.397433921870306	27.342608508382217	27.969372844019535	22.290584725727943
60-61	22.399284518689374	27.40534563135157	27.9649481171786	22.230421732780457
62-63	22.388761958134406	27.455571242280875	28.00019398851157	22.155472811073153
64-65	22.378948971332083	27.385407144667155	27.960038360653332	22.275605523347426
66-67	22.42640504442366	27.550725537760112	27.745325651741876	22.277543766074352
68-69	22.350719516000186	27.512921340441082	27.856267191265342	22.280091952293386
70-71	22.396681331624347	27.188261627016896	28.017006806522016	22.39805023483674
72-73	22.518816672852203	27.347882297815175	27.685889240040236	22.44741178929239
74-75	22.268564613032652	27.52652575795203	27.72685019582469	22.478059433190634
76-77	22.56644303280496	27.287661278106828	27.670261170348414	22.475634518739803
78-79	22.47836547754262	27.32363234231643	27.597685613055887	22.600316567085066
80-81	22.52286649961667	27.403509029762823	27.500300053152273	22.57332441746824
82-83	22.62262856467842	27.284816620167106	27.582464660550915	22.510090154603553
84-85	22.62584974889527	27.351811362453926	27.478405073440975	22.54393381520983
86-87	22.539059812804254	27.373978739160172	27.63864665487757	22.44831479315801
88-89	22.67155374795512	27.376128129279824	27.487766015827543	22.464552106937514
90-91	22.685445049176657	27.419959005911466	27.458214358943888	22.436381585967986
92-93	22.793178500131578	27.41300060844889	27.408810189088484	22.385010702331044
94-95	22.611510095473413	27.39960527700187	27.512721161972188	22.476163465552528
96-97	22.780751177658324	27.41675289202773	27.575431283186518	22.227064647127428
98-99	23.791654786331815	29.374671650868006	27.7530686248957	19.08060493790448
100	30.4517719922767	40.45724805645961	29.09097995126369	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1413.0
1	1256.0
2	1704.5
3	2537.5
4	3223.0
5	4633.5
6	5919.0
7	7182.0
8	8539.0
9	8977.0
10	8476.5
11	8001.0
12	8790.0
13	10926.5
14	14958.0
15	19634.0
16	23022.5
17	24874.5
18	24887.0
19	23031.5
20	20704.5
21	19216.5
22	19562.0
23	22693.5
24	28577.0
25	37142.0
26	48171.5
27	60194.0
28	70859.0
29	82629.5
30	95028.5
31	106865.0
32	122250.5
33	139885.0
34	154507.5
35	168453.0
36	184959.0
37	197833.0
38	207381.0
39	215488.5
40	223278.0
41	228164.5
42	230682.5
43	235923.5
44	241086.5
45	244929.5
46	246492.0
47	247184.0
48	247784.0
49	247843.0
50	245826.0
51	241400.5
52	234497.5
53	224449.5
54	212033.5
55	197790.0
56	180990.0
57	163010.5
58	145403.0
59	124762.5
60	103753.0
61	84328.5
62	66447.5
63	50895.0
64	37344.5
65	27209.5
66	19935.5
67	14955.0
68	11355.0
69	8436.0
70	6166.0
71	4277.0
72	2900.0
73	1965.5
74	1247.5
75	777.0
76	483.0
77	289.5
78	178.0
79	107.0
80	74.5
81	59.5
82	43.5
83	27.0
84	16.0
85	9.5
86	5.0
87	2.0
88	1.5
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018785662129248623
3	0.00248297447273547
4	1.6335358373259672E-5
5	0.0
6	4.5739003445127084E-4
7	0.0
8	0.0
9	0.0
10-11	1.6335358373259672E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.613739103260419E-4
24-25	0.0012415812419292113
26-27	0.003006186699551735
28-29	0.0018630195281216669
30-31	0.0025021869604659364
32-33	0.0034969914408063355
34-35	0.0012063053828546585
36-37	8.230462835962348E-6
38-39	4.960002949548421E-5
40-41	3.3230382838102323E-5
42-43	6.34657122314294E-4
44-45	0.0
46-47	0.0
48-49	2.0477129051901246E-4
50-51	0.006093093182387334
52-53	0.0
54-55	0.0032299679736922157
56-57	0.04924521645451274
58-59	0.061361271310120126
60-61	0.039711401973722596
62-63	0.008638927149418057
64-65	0.0
66-67	0.0
68-69	5.163346143349241E-4
70-71	0.002989148274325591
72-73	0.010993563264006802
74-75	0.0050595926031224234
76-77	0.004112674236224147
78-79	0.004098132743460572
80-81	0.016574882763561942
82-83	0.056762199366355175
84-85	0.03676403982447348
86-87	0.009801929209840084
88-89	0.008806281445891179
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	109.0
22-23	257.0
24-25	409.0
26-27	1155.0
28-29	2969.0
30-31	8942.0
32-33	11447.0
34-35	15508.0
36-37	25342.0
38-39	29406.0
40-41	30502.0
42-43	35261.0
44-45	43915.0
46-47	45278.0
48-49	42960.0
50-51	39029.0
52-53	36433.0
54-55	39787.0
56-57	42315.0
58-59	45991.0
60-61	45698.0
62-63	44710.0
64-65	47163.0
66-67	51084.0
68-69	52954.0
70-71	53620.0
72-73	49986.0
74-75	50571.0
76-77	53836.0
78-79	56691.0
80-81	56516.0
82-83	53294.0
84-85	52413.0
86-87	53533.0
88-89	57223.0
90-91	58196.0
92-93	57792.0
94-95	65414.0
96-97	193019.0
98-99	1443211.0
100-101	3027722.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.32400375192348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.08967981724088	79.54315542122251
2	6.486142054921011	11.327917862788622
3	1.4016592827480445	3.671955013968258
4	0.4860315806915205	1.6976889430343853
5	0.2134106937044061	0.9317938108872076
6	0.10330869120662792	0.5412797123120329
7	0.06534970634604105	0.39946186015041507
8	0.038999337191206046	0.27244626137659245
9	0.023809711812549463	0.18712434272861578
>10	0.08882008146008381	1.196174739489428
>50	0.00235470722565402	0.14200895610970637
>100	3.7805504069353255E-4	0.057883470273991204
>500	5.628041124288969E-5	0.031109605658234247
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6335358373259672E-5	0.0	0.0	0.0
3	0.0	1.6335358373259672E-5	0.0	0.0	0.0
4	0.0	4.9006075119779014E-5	0.0	0.0	0.0
5	0.0	4.9006075119779014E-5	0.0	0.0	0.0
6	3.2670716746519345E-5	4.9006075119779014E-5	0.0	0.0	0.0
7	3.2670716746519345E-5	4.9006075119779014E-5	0.0	0.0	0.0
8	3.2670716746519345E-5	6.534143349303869E-5	0.0	1.6335358373259672E-5	0.0
9	3.2670716746519345E-5	2.286950172256354E-4	0.0	1.6335358373259672E-5	0.0
10-11	6.534143349303869E-5	2.286950172256354E-4	0.0	3.2670716746519345E-5	0.0
12-13	6.534143349303869E-5	2.286950172256354E-4	0.0	3.2670716746519345E-5	0.0
14-15	6.534143349303869E-5	3.5937788421171277E-4	0.0	3.2670716746519345E-5	0.0
16-17	9.801215023955803E-5	3.5937788421171277E-4	0.0	3.2670716746519345E-5	0.0
18-19	9.801215023955803E-5	3.920486009582321E-4	0.0	4.9006075119779014E-5	2.4503037559889507E-5
20-21	9.801215023955803E-5	4.2471931770475145E-4	0.0	4.9006075119779014E-5	4.9006075119779014E-5
22-23	1.143475086128177E-4	4.9822843038442E-4	0.0	4.9006075119779014E-5	4.9006075119779014E-5
24-25	1.388505461727072E-4	5.47234505504199E-4	0.0	6.534143349303869E-5	4.9006075119779014E-5
26-27	1.6335358373259672E-4	5.880729014373482E-4	0.0	1.3068286698607738E-4	4.9006075119779014E-5
28-29	1.6335358373259672E-4	5.880729014373482E-4	0.0	4.8189307201116033E-4	4.9006075119779014E-5
30-31	1.6335358373259672E-4	6.697496933036466E-4	0.0	0.001388505461727072	5.7173754306408855E-5
32-33	1.8785662129248623E-4	6.697496933036466E-4	0.0	0.0030138736198664093	7.350911267966852E-5
34-35	2.1235965885237572E-4	7.759295227298344E-4	0.0	0.005153805566763427	8.167679186629836E-5
36-37	2.286950172256354E-4	8.821093521560223E-4	0.0	0.009384663385437681	8.167679186629836E-5
38-39	2.286950172256354E-4	8.98444710529282E-4	0.0	0.022101739879020338	8.167679186629836E-5
40-41	2.286950172256354E-4	0.0010127922191420996	0.0	0.03598679449629105	8.167679186629836E-5
42-43	2.286950172256354E-4	0.0010209598983287294	0.0	0.04400745545756156	8.167679186629836E-5
44-45	2.4503037559889506E-4	0.001143475086128177	0.0	0.05367798761453128	8.167679186629836E-5
46-47	2.6136573397215476E-4	0.0012578225947409948	0.0	0.0644674918200693	8.167679186629836E-5
48-49	2.6136573397215476E-4	0.0012741579531142543	0.0	0.07518348691292764	8.167679186629836E-5
50-51	2.695334131587846E-4	0.001404840820100332	0.0	0.08602199719358543	8.167679186629836E-5
52-53	2.777010923454144E-4	0.0014211761784735915	0.0	0.122858230325286	8.167679186629836E-5
54-55	2.777010923454144E-4	0.0014211761784735915	0.0	0.16809900534002864	8.167679186629836E-5
56-57	2.777010923454144E-4	0.0014211761784735915	0.0	0.23446139873139607	8.167679186629836E-5
58-59	2.8586877153204425E-4	0.0014211761784735915	0.0	0.27480973391334745	8.167679186629836E-5
60-61	3.022041299053039E-4	0.0014783499327800003	0.0	0.289315532148802	8.167679186629836E-5
62-63	3.2670716746519343E-4	0.0014946852911532599	0.0	0.30165689539979973	8.167679186629836E-5
64-65	3.2670716746519343E-4	0.0016335358373259672	0.0	0.3082400448242234	8.167679186629836E-5
66-67	3.2670716746519343E-4	0.001641703516512597	0.0	0.3144719840436219	8.167679186629836E-5
68-69	3.2670716746519343E-4	0.0017315479875655254	0.0	0.321332834560391	8.167679186629836E-5
70-71	3.2670716746519343E-4	0.001747883345938785	0.0	0.32667449674844695	8.167679186629836E-5
72-73	3.2670716746519343E-4	0.0017642187043120446	0.0	0.33075016866257523	8.167679186629836E-5
74-75	3.2670716746519343E-4	0.0018132247794318236	0.0	0.3342214323168929	8.167679186629836E-5
76-77	3.4304252583845313E-4	0.001894901571298122	0.0	0.3375701807834111	8.167679186629836E-5
78-79	3.4304252583845313E-4	0.0019112369296713815	0.0	0.3400939936520797	8.167679186629836E-5
80-81	3.4304252583845313E-4	0.0019194046088580113	0.0	0.34097610300423575	8.167679186629836E-5
82-83	3.4304252583845313E-4	0.001943907646417901	0.0	0.3415968466224196	8.167679186629836E-5
84-85	3.4304252583845313E-4	0.0019602430047911605	0.0	0.3418582123563918	8.167679186629836E-5
86-87	3.4304252583845313E-4	0.00197657836316442	0.0	0.34203790129849765	8.167679186629836E-5
88	3.4304252583845313E-4	0.0020745905134039785	0.0	0.3420624043360575	8.167679186629836E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7440	0.0	55.063473	1
GTATCAA	13850	0.0	38.114677	1
TCAACGC	16885	0.0	30.70289	4
ATCAACG	17245	0.0	30.012585	3
CAACGCA	17460	0.0	29.617912	5
TATCAAC	17955	0.0	29.089386	2
AACGCAG	17915	0.0	28.913673	6
ACGCAGA	21010	0.0	24.633715	7
CGCAGAG	21880	0.0	23.673866	8
GTACATG	19825	0.0	22.680998	1
TACATGG	20255	0.0	21.796253	2
ACATGGG	19865	0.0	21.682995	3
GTGGTAT	3610	0.0	20.957966	1
GCAGAGT	25445	0.0	20.42459	9
TGGTATC	3340	0.0	20.206768	2
GAGTACT	14595	0.0	19.379889	12-13
CATGGGG	13710	0.0	18.781158	4
CAGAGTA	24715	0.0	18.610268	10-11
AGAGTAC	23130	0.0	18.07355	10-11
GTACTTT	16690	0.0	17.011631	14-15
>>END_MODULE