FastQCFastQC Report
Fri 27 May 2016
SRR2139174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4940240
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55850.051.7515561
GTATCAA102500.035.941431
TCAACGC118600.030.3093244
ATCAACG120200.029.9773963
CAACGCA121400.029.5753025
AACGCAG126200.028.4840376
TATCAAC128300.028.1515542
ACGCAGA145350.024.7020427
CGCAGAG149600.023.9719098
GTACATG158200.022.1601681
TACATGG160000.021.2211782
ACATGGG159550.020.9081923
TGCGCGA2554.46961E-420.60758694
GCAGAGT178450.020.1439389
GAGTACT93900.018.89244712-13
CATGGGG102600.018.5727624
CAGAGTA176300.018.21101410-11
AGAGTAC160900.017.73837310-11
TGGTATC26150.017.2041682
GTGGTAT29100.016.7727551
Produced by FastQC (version 0.11.5)