FastQCFastQC Report
Fri 27 May 2016
SRR2139173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421227
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77000.048.8066141
GTATCAA143000.037.364771
TCAACGC166150.031.182694
CAACGCA170300.030.3727665
ATCAACG172950.029.9820983
AACGCAG176100.029.4456
TATCAAC178100.029.3071082
ACGCAGA209400.024.7216227
CGCAGAG217050.023.7914128
GTACATG226850.023.6664181
TACATGG236850.022.3973582
ACATGGG234600.022.1031723
GCAGAGT259450.019.9526239
CATGGGG179500.019.7724674
GAGTACT132800.018.30353512-13
CAGAGTA253850.017.52268810-11
AGAGTAC228700.017.4841910-11
GTACTTT149200.016.2773414-15
ATGGGGG111950.015.6422075
AGTACTT146550.015.13255712-13
Produced by FastQC (version 0.11.5)