##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7421227
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.57713097847566	28.0	18.0	32.0	18.0	33.0
2	27.74870031599896	29.0	25.0	31.0	18.0	33.0
3	30.582366905095345	31.0	29.0	33.0	27.0	33.0
4	30.15040935953044	31.0	29.0	33.0	27.0	33.0
5	31.723375932308766	33.0	31.0	33.0	29.0	33.0
6	36.100580672171866	38.0	36.0	38.0	33.0	38.0
7	36.47332550264262	38.0	37.0	38.0	34.0	38.0
8	36.671100749242676	38.0	37.0	38.0	34.0	38.0
9	36.98255584959199	38.0	38.0	38.0	35.0	38.0
10-11	37.14507344944441	38.0	38.0	38.0	36.0	38.0
12-13	37.243046331826264	38.0	38.0	38.0	36.0	38.0
14-15	37.15757630106181	38.0	38.0	38.0	36.0	38.0
16-17	37.33977919015279	38.0	38.0	38.0	36.5	38.0
18-19	37.373253506461936	38.0	38.0	38.0	37.0	38.0
20-21	37.40863738202395	38.0	38.0	38.0	37.0	38.0
22-23	37.4300470239165	38.0	38.0	38.0	37.0	38.0
24-25	37.44833831124983	38.0	38.0	38.0	37.0	38.0
26-27	37.410190855513456	38.0	38.0	38.0	37.0	38.0
28-29	37.38783602539746	38.0	38.0	38.0	37.0	38.0
30-31	37.39524419965408	38.0	38.0	38.0	37.0	38.0
32-33	37.38685595401627	38.0	38.0	38.0	37.0	38.0
34-35	37.36252475610855	38.0	38.0	38.0	37.0	38.0
36-37	37.345108520518956	38.0	38.0	38.0	37.0	38.0
38-39	37.35652405328486	38.0	38.0	38.0	37.0	38.0
40-41	37.35476339609761	38.0	38.0	38.0	37.0	38.0
42-43	37.32144500383841	38.0	38.0	38.0	37.0	38.0
44-45	37.243977055375304	38.0	38.0	38.0	37.0	38.0
46-47	37.285618858399985	38.0	38.0	38.0	37.0	38.0
48-49	37.24805576456024	38.0	38.0	38.0	37.0	38.0
50-51	37.20784262889	38.0	38.0	38.0	37.0	38.0
52-53	37.191623502547905	38.0	38.0	38.0	37.0	38.0
54-55	37.17279118430405	38.0	38.0	38.0	36.0	38.0
56-57	37.13872838474357	38.0	38.0	38.0	36.0	38.0
58-59	37.131701949071854	38.0	38.0	38.0	36.0	38.0
60-61	37.14187050006358	38.0	38.0	38.0	36.0	38.0
62-63	37.15011256663417	38.0	38.0	38.0	36.0	38.0
64-65	37.112891321152816	38.0	38.0	38.0	36.0	38.0
66-67	37.143370604966066	38.0	38.0	38.0	36.0	38.0
68-69	37.13353891817815	38.0	38.0	38.0	36.0	38.0
70-71	37.13676844112753	38.0	38.0	38.0	36.0	38.0
72-73	37.12308398263008	38.0	38.0	38.0	36.0	38.0
74-75	37.11831469024989	38.0	38.0	38.0	36.0	38.0
76-77	37.10776708023795	38.0	38.0	38.0	36.0	38.0
78-79	37.08959018222423	38.0	38.0	38.0	36.0	38.0
80-81	37.06012110794238	38.0	38.0	38.0	36.0	38.0
82-83	37.0343887014126	38.0	38.0	38.0	36.0	38.0
84-85	37.01417261648905	38.0	38.0	38.0	36.0	38.0
86-87	37.009271715353094	38.0	38.0	38.0	36.0	38.0
88-89	36.994825657871516	38.0	38.0	38.0	36.0	38.0
90-91	36.974516307649935	38.0	38.0	38.0	36.0	38.0
92-93	36.95206265600992	38.0	38.0	38.0	35.5	38.0
94-95	36.9272437164912	38.0	38.0	38.0	35.0	38.0
96-97	36.93401729406955	38.0	38.0	38.0	35.0	38.0
98-99	36.93819962817986	38.0	38.0	38.0	35.0	38.0
100	35.627793892777085	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	20.0
21	94.0
22	464.0
23	1322.0
24	3248.0
25	6544.0
26	11614.0
27	19217.0
28	29344.0
29	41856.0
30	57300.0
31	75884.0
32	101491.0
33	141971.0
34	223612.0
35	441968.0
36	1422477.0
37	4842795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0860189292148	20.60525840268732	13.112454315169176	24.1962683529287
2	15.729835775042005	20.577647440723418	40.967532984660906	22.724983799573675
3	18.470692914088236	26.71455854928857	29.643697275617463	25.17105126100573
4	13.336797270855616	17.508371594077367	37.97275032821392	31.182080806853097
5	13.991284729600645	37.34971319432756	35.111134587312854	13.547867488758936
6	30.78036935041828	37.329897586519465	18.423538755436965	13.466194307625285
7	26.98109086273739	32.267938980979835	23.410966946570966	17.340003209711817
8	24.65850997415926	37.12224407096023	20.85230649864234	17.366939456238168
9	26.292687179626768	16.615742922295734	21.02560668202172	36.06596321605578
10-11	24.975160773648504	26.259598229429898	28.61223527030677	20.15300572661483
12-13	25.568332568185824	23.989078894905113	28.736562026737626	21.70602651017143
14-15	22.902149469353247	25.12481426588892	25.93166197449559	26.041374290262244
16-17	22.567380030283402	28.077365104180213	26.764219717305508	22.59103514823088
18-19	22.684510256861838	27.443292059385865	28.162054603638992	21.710143080113305
20-21	23.33015594405585	26.67850843807062	27.974992410243804	22.016343207629724
22-23	23.305737693486204	26.516682736966974	27.788701299865064	22.38887826968176
24-25	22.94870230462204	26.715118686313488	27.857110563197455	22.479068445867018
26-27	22.825489860471134	26.778389939581963	27.91013979840889	22.48598040153801
28-29	22.880609916289384	26.73745769712761	27.905889185347515	22.476043201235495
30-31	23.04582958538221	26.73090575618502	27.7273407133985	22.495923945034264
32-33	22.879574930101594	26.90631917258352	27.721251886117887	22.492854011196993
34-35	23.03857548346666	26.77003553335851	27.748898684085965	22.442490299088867
36-37	22.995982296285227	26.73711732904151	27.712021423037452	22.554878951635814
38-39	22.928012616958895	26.88867626571392	27.803392107151137	22.379919010176046
40-41	23.065596322320975	26.693828046706436	27.732537864410634	22.508037766561955
42-43	22.892458409703632	26.86274698506224	27.542846239161	22.701948366073122
44-45	22.948802209706656	26.87763526242184	27.475939419952	22.697623107919497
46-47	23.108289396587807	26.908104482908634	27.261014117018195	22.72259200348536
48-49	23.016496381499277	27.012154691118607	27.157515812329162	22.81383311505295
50-51	23.003743268381545	27.124962181718015	27.133424614199917	22.737869935700523
52-53	23.163072594913974	26.94330841521348	27.090828408139895	22.802790581732648
54-55	23.162263861837744	26.845694893671173	27.06096580840279	22.931075436088292
56-57	23.036441104943393	27.050661145677473	27.158270959212345	22.754626790166792
58-59	23.091235888285937	27.052425001129738	27.06481516213094	22.791523948453385
60-61	23.12309127358062	27.03251154508564	27.140655950936406	22.703741230397338
62-63	23.207003806596223	27.06750897199371	27.11222530603539	22.613261915374675
64-65	23.193807695558615	27.06731530983486	26.991470956323525	22.747406038282993
66-67	23.143091658863572	27.127711654107955	26.916353530073533	22.812843156954944
68-69	23.096226545872582	27.023603751271054	27.077039187478803	22.803130515377564
70-71	23.139393847725394	26.80593283286228	27.110302418108333	22.944370901303994
72-73	23.290504152165134	26.954154720348072	26.828701150019935	22.926639977466863
74-75	22.953239453278325	27.095692508879054	27.03244500678577	22.918623031056853
76-77	23.302102295894617	26.85750427811435	26.940441905936062	22.899951520054966
78-79	23.0361500930655	26.838490667457766	26.97443742025405	23.15092181922269
80-81	23.163056002062334	26.975113292097646	26.880909564093137	22.980921141746887
82-83	23.174431906838674	26.91096509053657	26.98823693670524	22.926366065919517
84-85	23.18075351315534	26.89313756789052	26.92307756729423	23.003031351659907
86-87	23.137547282311864	26.88394083222283	27.04419648325856	22.934315402206746
88-89	23.149788685459093	26.87653102231903	26.927716546625135	23.04596374559674
90-91	23.201924494376424	26.96463559044611	26.917140921268544	22.916298993908917
92-93	23.281107525244934	26.892572442661717	26.914482124876788	22.91183790721657
94-95	23.075487327418667	26.86339383149271	26.996429958091582	23.06468888299704
96-97	23.29267600686329	26.918806940747846	27.01301052460921	22.775506527779655
98-99	24.408691126419573	28.886718673855665	27.090102260012554	19.614487939712205
100	31.562882174584168	39.97942067273303	28.457697152682798	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	894.0
1	844.5
2	1199.5
3	1798.5
4	2369.0
5	3536.0
6	4533.0
7	5349.5
8	6148.5
9	6450.0
10	6347.5
11	6209.0
12	6887.0
13	8640.0
14	11939.0
15	15927.5
16	18950.0
17	20779.5
18	21161.0
19	20066.5
20	18644.5
21	17846.0
22	18517.0
23	21754.5
24	27368.5
25	35483.5
26	45721.5
27	57594.5
28	71547.5
29	87157.0
30	102419.0
31	118698.5
32	136883.5
33	155854.0
34	170899.5
35	185112.5
36	202937.5
37	220530.0
38	237616.0
39	249995.5
40	260404.5
41	270839.0
42	278249.0
43	285063.0
44	294300.0
45	303737.0
46	308869.5
47	313141.0
48	316125.0
49	316813.5
50	318372.0
51	318095.0
52	312086.0
53	301955.5
54	289762.5
55	274182.5
56	254274.5
57	231781.0
58	207400.5
59	177882.0
60	147910.5
61	120513.0
62	95463.0
63	73729.5
64	54466.0
65	39756.0
66	29349.5
67	22073.5
68	16501.5
69	12110.5
70	8810.5
71	6170.0
72	4333.5
73	2868.0
74	1812.5
75	1161.0
76	746.5
77	452.5
78	287.0
79	168.5
80	104.0
81	73.5
82	48.0
83	33.0
84	22.0
85	12.5
86	5.0
87	3.0
88	3.5
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016978324473837009
3	0.0027758213028654154
4	0.0
5	0.0
6	4.8509498496677165E-4
7	0.0
8	0.0
9	0.0
10-11	2.0212291040282154E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.737596035167287E-5
24-25	0.0010915330656022827
26-27	0.0028233818332831437
28-29	0.0018198801278661257
30-31	0.0024016894401021013
32-33	0.00308907534646819
34-35	9.82566958211224E-4
36-37	6.801482015725299E-6
38-39	5.4713581921757115E-5
40-41	6.881059848362084E-6
42-43	5.608092380374889E-4
44-45	0.0
46-47	0.0
48-49	2.410626381412993E-4
50-51	0.0061820536911005725
52-53	0.0
54-55	0.003025251271784439
56-57	0.04757217667763614
58-59	0.059272220957866245
60-61	0.03875906084249363
62-63	0.008561010529667469
64-65	0.0
66-67	0.0
68-69	6.792267004015928E-4
70-71	0.0029314175376264203
72-73	0.010432377564829549
74-75	0.005142519728160207
76-77	0.00405320859617321
78-79	0.0038845054619066253
80-81	0.016756911324130475
82-83	0.05512113640359942
84-85	0.03634190321902531
86-87	0.00977867376144976
88-89	0.008585773458894285
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	24.0
20-21	103.0
22-23	265.0
24-25	450.0
26-27	1474.0
28-29	4201.0
30-31	13721.0
32-33	17138.0
34-35	23579.0
36-37	38289.0
38-39	44988.0
40-41	43835.0
42-43	48753.0
44-45	58238.0
46-47	59707.0
48-49	56944.0
50-51	52983.0
52-53	50910.0
54-55	56122.0
56-57	59130.0
58-59	59545.0
60-61	58827.0
62-63	55948.0
64-65	58626.0
66-67	63288.0
68-69	64617.0
70-71	64164.0
72-73	59790.0
74-75	60298.0
76-77	62942.0
78-79	66814.0
80-81	65764.0
82-83	61986.0
84-85	61178.0
86-87	63377.0
88-89	66462.0
90-91	67946.0
92-93	66746.0
94-95	77217.0
96-97	235142.0
98-99	1756770.0
100-101	3592926.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.66160116500566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49375266467122	73.1503528836171
2	7.704142394319452	12.736734918352926
3	2.0596024380369773	5.107501058744577
4	0.7669140806624235	2.5357738345417697
5	0.37807554824292494	1.5626165089548758
6	0.203699631639672	1.0102882624834282
7	0.11460158801617044	0.6631205533028282
8	0.06713776843309142	0.44397723498597674
9	0.044431949437154575	0.3305534475021992
>10	0.16250582565592375	2.073545484917125
>50	0.0037027732088975246	0.2130632495553253
>100	0.0014333376760303639	0.17247256304188044
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3474860693521435E-5
2	0.0	8.084916416112862E-5	0.0	0.0	1.3474860693521435E-5
3	0.0	8.084916416112862E-5	0.0	0.0	1.3474860693521435E-5
4	0.0	2.0212291040282152E-4	0.0	0.0	1.3474860693521435E-5
5	0.0	2.1559777109634296E-4	0.0	1.3474860693521435E-5	1.3474860693521435E-5
6	0.0	2.1559777109634296E-4	0.0	1.3474860693521435E-5	2.694972138704287E-5
7	0.0	2.1559777109634296E-4	0.0	1.3474860693521435E-5	2.694972138704287E-5
8	0.0	2.5602235317690727E-4	0.0	1.3474860693521435E-5	2.694972138704287E-5
9	0.0	5.120447063538145E-4	0.0	1.3474860693521435E-5	2.694972138704287E-5
10-11	0.0	5.120447063538145E-4	0.0	1.3474860693521435E-5	2.694972138704287E-5
12-13	0.0	5.120447063538145E-4	0.0	1.3474860693521435E-5	3.3687151733803586E-5
14-15	0.0	6.939553257163539E-4	0.0	1.3474860693521435E-5	8.758659450788933E-5
16-17	0.0	7.006927560631146E-4	0.0	1.3474860693521435E-5	1.3474860693521434E-4
18-19	0.0	8.017542112645254E-4	0.0	1.3474860693521435E-5	1.5496089797549653E-4
20-21	0.0	9.29765387852979E-4	0.0	1.3474860693521435E-5	1.886480497093001E-4
22-23	0.0	0.0010173519823608683	0.0	1.3474860693521435E-5	2.2233520144310367E-4
24-25	0.0	0.00121947489276369	0.0	1.3474860693521435E-5	2.5602235317690727E-4
26-27	0.0	0.0013474860693521436	0.0	2.0212291040282154E-5	2.694972138704287E-4
28-29	0.0	0.0013474860693521436	0.0	2.3581006213662512E-4	2.964469352574716E-4
30-31	0.0	0.0015293966887146829	0.0	0.0011386257286025612	3.031843656042323E-4
32-33	0.0	0.0015563464101017257	0.0	0.0027623464421718943	3.368715173380359E-4
34-35	0.0	0.0017584693205045473	0.0	0.004594927496490809	4.0424582080564303E-4
36-37	0.0	0.0019605922309073685	0.0	0.007593084000799329	4.0424582080564303E-4
38-39	0.0	0.002034703964721737	0.0	0.012619207039482824	4.0424582080564303E-4
40-41	0.0	0.0022637765965116013	0.0	0.017840715558222378	4.1098325115240374E-4
42-43	0.0	0.0023917877731000547	0.0	0.022893788318292918	4.244581118459252E-4
44-45	0.0	0.002674759847664005	0.0	0.02883620188413587	4.311955421926859E-4
46-47	0.0	0.002883620188413587	0.0	0.035203073561824755	4.311955421926859E-4
48-49	0.0	0.002890357618760348	0.0	0.0420213530727466	4.581452635797288E-4
50-51	0.0	0.003247441427138666	0.0	0.04923040354378057	4.7162012427325023E-4
52-53	0.0	0.003274391148525709	0.0	0.05546926404488099	5.187821367005753E-4
54-55	0.0	0.003294603439565991	0.0	0.06180918600118282	5.72681579474661E-4
56-57	0.0	0.0033552403126868376	0.0	0.06822321969129902	6.063687312084646E-4
58-59	0.0	0.0033754526037271197	0.0	0.07493370031667271	6.19843591901986E-4
60-61	0.0	0.003436089476847966	0.0	0.08132078428540186	6.333184525955074E-4
62-63	0.0	0.0034765140589285305	0.0	0.08751922020442171	6.333184525955074E-4
64-65	0.0	0.003914447031467977	0.0	0.09338752203645032	6.333184525955074E-4
66-67	0.0	0.0039818213349355844	0.0	0.10028665071153328	6.400558829422682E-4
68-69	0.0	0.004082882790136995	0.0	0.10626275142911004	6.602681739825504E-4
70-71	0.0	0.0041502570936046024	0.0	0.11197609236316314	6.872178953695932E-4
72-73	0.0	0.004170469384644884	0.0	0.11583663995185703	7.41117338143679E-4
74-75	0.0	0.0043052179915800985	0.0	0.1188415338865123	7.478547684904397E-4
76-77	0.0	0.004547765484063484	0.0	0.12192727698532871	7.680670595307218E-4
78-79	0.0	0.004675776660651938	0.0	0.12433253961912227	7.680670595307218E-4
80-81	0.0	0.00468925152134546	0.0	0.12518819327316089	7.748044898774826E-4
82-83	0.0	0.004729676103426023	0.0	0.12564633853674062	7.882793505710039E-4
84-85	0.0	0.004824000128280674	0.0	0.12588214859887725	7.950167809177647E-4
86-87	0.0	0.004985698456602931	0.0	0.12591583575061105	8.017542112645254E-4
88	0.0	0.005241720809779838	0.0	0.12592257318095781	8.219665023048076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7700	0.0	48.806614	1
GTATCAA	14300	0.0	37.36477	1
TCAACGC	16615	0.0	31.18269	4
CAACGCA	17030	0.0	30.372766	5
ATCAACG	17295	0.0	29.982098	3
AACGCAG	17610	0.0	29.445	6
TATCAAC	17810	0.0	29.307108	2
ACGCAGA	20940	0.0	24.721622	7
CGCAGAG	21705	0.0	23.791412	8
GTACATG	22685	0.0	23.666418	1
TACATGG	23685	0.0	22.397358	2
ACATGGG	23460	0.0	22.103172	3
GCAGAGT	25945	0.0	19.952623	9
CATGGGG	17950	0.0	19.772467	4
GAGTACT	13280	0.0	18.303535	12-13
CAGAGTA	25385	0.0	17.522688	10-11
AGAGTAC	22870	0.0	17.48419	10-11
GTACTTT	14920	0.0	16.27734	14-15
ATGGGGG	11195	0.0	15.642207	5
AGTACTT	14655	0.0	15.132557	12-13
>>END_MODULE