FastQCFastQC Report
Fri 27 May 2016
SRR2139172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6934668
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81600.051.8855321
GTATCAA145000.035.7695471
TCAACGC167200.029.9970784
ATCAACG169200.029.6683943
CAACGCA171300.029.2791065
AACGCAG175950.028.6752856
TATCAAC179300.028.2596762
ACGCAGA205050.024.459597
CGCAGAG212200.023.6153018
GTACATG201900.020.6526741
TACATGG200700.020.1375472
GCAGAGT251650.020.0320749
GAGTACT141050.019.70221312-13
ACATGGG202150.019.5278633
CAGAGTA244300.018.77238310-11
CATGGGG147700.018.0778434
AGAGTAC225350.017.61160710-11
GTGGTAT42350.017.3515321
GTACTTT163900.017.03382314-15
TGGTATC38350.016.821872
Produced by FastQC (version 0.11.5)