##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6934668
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.788159865764303	28.0	18.0	32.0	18.0	33.0
2	27.728480296389098	29.0	25.0	31.0	18.0	33.0
3	30.625245505624783	31.0	29.0	33.0	27.0	33.0
4	30.11485365990124	31.0	29.0	33.0	25.0	33.0
5	31.752695875274778	33.0	31.0	33.0	29.0	33.0
6	36.135481323691344	38.0	36.0	38.0	33.0	38.0
7	36.5003537299839	38.0	37.0	38.0	34.0	38.0
8	36.692523275807865	38.0	37.0	38.0	34.0	38.0
9	36.991342051270514	38.0	38.0	38.0	35.0	38.0
10-11	37.15751006392807	38.0	38.0	38.0	36.0	38.0
12-13	37.25640910278618	38.0	38.0	38.0	36.0	38.0
14-15	37.16561975281297	38.0	38.0	38.0	36.0	38.0
16-17	37.35645715122916	38.0	38.0	38.0	36.5	38.0
18-19	37.38695147337984	38.0	38.0	38.0	37.0	38.0
20-21	37.42354446535424	38.0	38.0	38.0	37.0	38.0
22-23	37.44338542428904	38.0	38.0	38.0	37.0	38.0
24-25	37.458800970013755	38.0	38.0	38.0	37.0	38.0
26-27	37.421903940322125	38.0	38.0	38.0	37.0	38.0
28-29	37.400266722487686	38.0	38.0	38.0	37.0	38.0
30-31	37.40667831625072	38.0	38.0	38.0	37.0	38.0
32-33	37.395758575215666	38.0	38.0	38.0	37.0	38.0
34-35	37.36912000082718	38.0	38.0	38.0	37.0	38.0
36-37	37.34868337145952	38.0	38.0	38.0	37.0	38.0
38-39	37.35805407354937	38.0	38.0	38.0	37.0	38.0
40-41	37.353325519494405	38.0	38.0	38.0	37.0	38.0
42-43	37.31661644011855	38.0	38.0	38.0	37.0	38.0
44-45	37.23541313332558	38.0	38.0	38.0	37.0	38.0
46-47	37.27319418169173	38.0	38.0	38.0	37.0	38.0
48-49	37.2339046383397	38.0	38.0	38.0	37.0	38.0
50-51	37.19227715117026	38.0	38.0	38.0	37.0	38.0
52-53	37.17591530981227	38.0	38.0	38.0	37.0	38.0
54-55	37.1561063174712	38.0	38.0	38.0	36.0	38.0
56-57	37.119045546490504	38.0	38.0	38.0	36.0	38.0
58-59	37.109244855332236	38.0	38.0	38.0	36.0	38.0
60-61	37.12201534593884	38.0	38.0	38.0	36.0	38.0
62-63	37.131745399528526	38.0	38.0	38.0	36.0	38.0
64-65	37.09617754589621	38.0	38.0	38.0	36.0	38.0
66-67	37.12888778762192	38.0	38.0	38.0	36.0	38.0
68-69	37.1185476976391	38.0	38.0	38.0	36.0	38.0
70-71	37.1238830970606	38.0	38.0	38.0	36.0	38.0
72-73	37.11047508672953	38.0	38.0	38.0	36.0	38.0
74-75	37.10648402219579	38.0	38.0	38.0	36.0	38.0
76-77	37.100109268830906	38.0	38.0	38.0	36.0	38.0
78-79	37.08214175825723	38.0	38.0	38.0	36.0	38.0
80-81	37.05338852669307	38.0	38.0	38.0	36.0	38.0
82-83	37.025587358661184	38.0	38.0	38.0	36.0	38.0
84-85	37.00968560128001	38.0	38.0	38.0	36.0	38.0
86-87	37.00459446502019	38.0	38.0	38.0	36.0	38.0
88-89	36.991712639768274	38.0	38.0	38.0	36.0	38.0
90-91	36.97351601162185	38.0	38.0	38.0	36.0	38.0
92-93	36.95153236789665	38.0	38.0	38.0	35.5	38.0
94-95	36.92967867809332	38.0	38.0	38.0	35.0	38.0
96-97	36.934349882584115	38.0	38.0	38.0	35.0	38.0
98-99	36.93698947669539	38.0	38.0	38.0	35.0	38.0
100	35.62046557404259	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	29.0
21	111.0
22	358.0
23	1164.0
24	2856.0
25	6193.0
26	10870.0
27	18176.0
28	27961.0
29	40475.0
30	54815.0
31	72348.0
32	94986.0
33	131527.0
34	207453.0
35	409929.0
36	1316727.0
37	4538683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.610759159631	21.10983539514797	13.421392343512336	23.858013101708693
2	15.277988478530347	21.029782543364135	41.66223474057082	22.0299942375347
3	17.960159100564397	27.280523620942414	30.331220125742632	24.428097152750556
4	12.756904872735076	18.12359293912845	39.26637872209599	29.853123466040483
5	13.322757484568836	37.87150300490232	35.881126536987786	12.924612973541056
6	29.600735439912036	38.50648016295041	18.97039854354057	12.922385853596985
7	26.086612942393206	32.98108864043672	24.004537780323442	16.927760636846635
8	23.97297174140132	37.58474089891542	21.396107210900364	17.046180148782895
9	25.97974409157007	16.907586635726467	21.48643597645915	35.62623329624432
10-11	24.16074022294508	26.778645118889983	29.408583756899663	19.652030901265274
12-13	25.007339933216706	24.521606513823013	29.384528574403273	21.086524978557012
14-15	22.222873539151404	25.824956580473646	26.821651735887	25.130518144487958
16-17	21.762015139008817	28.794824784690487	27.54845653750115	21.89470353879955
18-19	21.81893927726605	28.076499120073233	29.102308863236136	21.002252739424584
20-21	22.640113782829154	27.32364771853199	28.904377374140793	21.131861124498062
22-23	22.397614043760463	27.17641247226423	28.767311943819884	21.658661540155432
24-25	22.133663187135934	27.331377169911487	28.8100082766307	21.72495136632189
26-27	22.026508060044897	27.455009308816752	28.830829058739297	21.687653572399054
28-29	22.061808387261944	27.469135156803365	28.820034485552043	21.64902197038265
30-31	22.294592795495994	27.361448236128215	28.711141177608766	21.632817790767017
32-33	22.12621974533212	27.522494756322903	28.63805731293139	21.713228185413584
34-35	22.22029926457383	27.49541033833751	28.653452974972975	21.630837422115683
36-37	22.233819099817296	27.36113135208865	28.650810044527404	21.75423950356665
38-39	22.091778966277932	27.593331070761923	28.695778743921778	21.619111219038366
40-41	22.290145570561766	27.41043076426421	28.61596801635261	21.68345564882141
42-43	22.181844312816605	27.536668522007922	28.402262554247642	21.87922461092783
44-45	22.254700021172425	27.637362346634998	28.28009187684639	21.827845755346186
46-47	22.335534645304246	27.577612589631016	28.088998724897117	21.99785404016762
48-49	22.261128689390006	27.68277039365488	28.06379148867539	21.992309428279725
50-51	22.230539499367616	27.79415655289074	28.005369072174396	21.969934875567247
52-53	22.45217928699134	27.696307787527207	27.875833490218373	21.975679435263082
54-55	22.42713118575358	27.627991548234853	27.8588879704134	22.085989295598168
56-57	22.37517749387095	27.76863865405405	27.89930884465011	21.956875007424888
58-59	22.371697069542083	27.758562674157954	27.885132697482035	21.984607558817927
60-61	22.419988667232698	27.734177198081017	27.921779258777867	21.924054875908425
62-63	22.410722687475264	27.80843989552526	27.94401884046378	21.836818576535695
64-65	22.372498651108643	27.77583898779322	27.883144143710194	21.968518217387945
66-67	22.439494827532073	27.914126715575634	27.70452850392956	21.941849952962738
68-69	22.444623531589723	27.810007424814692	27.814726167958923	21.93064287563666
70-71	22.45268249463363	27.500950504796855	27.91265985885368	22.133707141715835
72-73	22.52037420909295	27.637618204516052	27.756585005193614	22.085422581197385
74-75	22.275884710353985	27.80139130747393	27.788223691093993	22.134500291078087
76-77	22.46721706740023	27.606563367778474	27.75223199301873	22.173987571802567
78-79	22.373736132845075	27.62894576747682	27.681744598652752	22.31557350102535
80-81	22.397153988999055	27.68497309235027	27.66471790674726	22.25315501190342
82-83	22.477684994231904	27.61091005749703	27.791486916600388	22.119918031670675
84-85	22.500491863466383	27.54822115110251	27.72011213861878	22.231174846812326
86-87	22.330270854501148	27.650726037793856	27.763445634648313	22.25555747305668
88-89	22.468178338819097	27.58675530218153	27.685971439978157	22.259094919021212
90-91	22.4986862947498	27.63092247704717	27.714121503600108	22.15626972460292
92-93	22.552150930494633	27.633365734092752	27.64096126586722	22.1735220695454
94-95	22.4175076678478	27.621880845662837	27.73191185319958	22.228699633289775
96-97	22.540424783303354	27.692292631483777	27.772067748576703	21.995214836636166
98-99	23.59914852726584	29.63509526478761	27.960570945619345	18.805185262327207
100	30.038619583658914	40.76335628027017	29.198024136070917	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	950.0
1	913.0
2	1212.5
3	1738.0
4	2369.0
5	3505.0
6	4484.0
7	5352.0
8	6208.5
9	6544.0
10	6428.5
11	6399.0
12	7128.5
13	9061.5
14	12931.0
15	17527.5
16	20936.5
17	23058.0
18	23698.5
19	22810.0
20	21447.0
21	20857.5
22	22102.0
23	26025.5
24	32971.5
25	42899.5
26	56015.0
27	70887.0
28	86069.0
29	103040.0
30	119706.0
31	135330.5
32	153034.5
33	171419.0
34	187448.5
35	203755.5
36	223217.5
37	238487.5
38	249359.0
39	259034.5
40	268567.0
41	276543.0
42	279348.0
43	281850.5
44	286463.0
45	289542.5
46	288698.5
47	287551.0
48	286572.0
49	283535.0
50	278736.0
51	271790.5
52	260999.5
53	246421.0
54	229546.0
55	212494.0
56	193453.5
57	173101.5
58	152484.5
59	129472.0
60	107616.5
61	86915.0
62	67977.0
63	51892.0
64	38105.0
65	27831.5
66	20221.5
67	14969.0
68	11262.5
69	8324.0
70	6060.0
71	4239.5
72	2866.0
73	1835.5
74	1216.0
75	773.0
76	476.5
77	298.5
78	175.5
79	101.5
80	67.0
81	48.5
82	36.0
83	27.5
84	19.0
85	10.5
86	4.0
87	2.0
88	2.5
89	3.0
90	2.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019611609380578853
3	0.002682175988814461
4	0.0
5	0.0
6	6.20072943650655E-4
7	0.0
8	0.0
9	0.0
10-11	7.210150507565755E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.3073285151987695E-4
24-25	0.0011032501533193228
26-27	0.0030143849623439855
28-29	0.0019115794248266702
30-31	0.0026784777669379245
32-33	0.003659843445495239
34-35	0.0012035459363395253
36-37	0.0
38-39	6.582908181084687E-5
40-41	1.4714052980007722E-5
42-43	5.846473961951148E-4
44-45	0.0
46-47	0.0
48-49	1.666623612223351E-4
50-51	0.006374985016876532
52-53	0.0
54-55	0.00305259599659008
56-57	0.04840245343255888
58-59	0.060622860097346416
60-61	0.03910159476490047
62-63	0.008559616849750032
64-65	0.0
66-67	0.0
68-69	6.835130959050402E-4
70-71	0.0027530818297430986
72-73	0.01020475269348778
74-75	0.005002256950508857
76-77	0.004109684746083128
78-79	0.004074592201230526
80-81	0.016448073144520063
82-83	0.05512154446378456
84-85	0.03638866919691118
86-87	0.00925487019503331
88-89	0.008525659911152592
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	28.0
20-21	154.0
22-23	329.0
24-25	519.0
26-27	1433.0
28-29	3743.0
30-31	12167.0
32-33	15374.0
34-35	21023.0
36-37	33927.0
38-39	40087.0
40-41	39295.0
42-43	44424.0
44-45	53763.0
46-47	54967.0
48-49	52085.0
50-51	48346.0
52-53	46781.0
54-55	52111.0
56-57	54605.0
58-59	54592.0
60-61	53384.0
62-63	51619.0
64-65	54693.0
66-67	58705.0
68-69	60045.0
70-71	59381.0
72-73	55847.0
74-75	56695.0
76-77	59345.0
78-79	61823.0
80-81	61439.0
82-83	57247.0
84-85	57193.0
86-87	58751.0
88-89	62197.0
90-91	62737.0
92-93	62191.0
94-95	70963.0
96-97	213614.0
98-99	1604948.0
100-101	3422098.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.65811270348414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64490889662818	75.89168800664315
2	7.093668826276025	12.010732299521353
3	1.7950972694080751	4.559086408417963
4	0.6638987758709649	2.248176695655572
5	0.3170252873212472	1.3419381251948292
6	0.16278291891919386	0.8268536817637938
7	0.09431417618930862	0.5589122110159525
8	0.05758253596035497	0.3899863055267327
9	0.04020414506337197	0.30632463395299525
>10	0.12800085842964096	1.6806106485449341
>50	0.002133546772327237	0.12000149089492226
>100	3.827631613060555E-4	0.06568949286777456
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4420301015131511E-5	0.0	0.0	0.0
3	0.0	1.4420301015131511E-5	0.0	0.0	0.0
4	0.0	5.7681204060526044E-5	0.0	0.0	0.0
5	0.0	5.7681204060526044E-5	0.0	0.0	0.0
6	0.0	7.210150507565755E-5	0.0	1.4420301015131511E-5	2.8840602030263022E-5
7	0.0	7.210150507565755E-5	0.0	1.4420301015131511E-5	2.8840602030263022E-5
8	0.0	1.2978270913618358E-4	0.0	1.4420301015131511E-5	2.8840602030263022E-5
9	0.0	2.5956541827236717E-4	0.0	5.7681204060526044E-5	2.8840602030263022E-5
10-11	0.0	2.5956541827236717E-4	0.0	8.652180609078907E-5	2.8840602030263022E-5
12-13	0.0	2.5956541827236717E-4	0.0	1.2257255862861783E-4	4.3260903045394535E-5
14-15	0.0	4.181887294388138E-4	0.0	1.442030101513151E-4	7.210150507565755E-5
16-17	0.0	4.398191809615111E-4	0.0	1.442030101513151E-4	7.210150507565755E-5
18-19	0.0	5.047105355296029E-4	0.0	1.730436121815781E-4	7.210150507565755E-5
20-21	0.0	5.696018900976946E-4	0.0	1.946740637042754E-4	7.210150507565755E-5
22-23	0.0	6.056526426355235E-4	0.0	2.0188421421184116E-4	1.0094210710592056E-4
24-25	0.0	6.48913545680918E-4	0.0	2.0188421421184116E-4	1.442030101513151E-4
26-27	0.0	6.77754147711181E-4	0.0	2.3072481624210418E-4	1.5141316065888085E-4
28-29	0.0	6.77754147711181E-4	0.0	4.68659782991774E-4	1.586233111664466E-4
30-31	0.0	8.075368568473646E-4	0.0	0.0011896748337483496	1.586233111664466E-4
32-33	0.0	8.075368568473646E-4	0.0	0.0024947120756177513	1.730436121815781E-4
34-35	0.0	9.517398669986797E-4	0.0	0.0043405106055545845	1.730436121815781E-4
36-37	0.0	0.0010815225761348633	0.0	0.0074120347217775965	1.730436121815781E-4
38-39	0.0	0.0011031530276575606	0.0	0.012033741197127246	1.730436121815781E-4
40-41	0.0	0.00124014588730131	0.0	0.016489614210802882	1.8025376268914387E-4
42-43	0.0	0.00129061694085427	0.0	0.02123389324478115	1.946740637042754E-4
44-45	0.0	0.0014203996499904537	0.0	0.026353100105152835	2.090943647194069E-4
46-47	0.0	0.0015141316065888086	0.0	0.031991437802069256	2.3072481624210418E-4
48-49	0.0	0.0015213417570963744	0.0	0.03755046384340245	2.3072481624210418E-4
50-51	0.0	0.0017592767238460442	0.0	0.04321043199184157	2.3072481624210418E-4
52-53	0.0	0.0017592767238460442	0.0	0.04830079825018299	2.739857192874987E-4
54-55	0.0	0.0017809071753687416	0.0	0.0540689186562356	3.172466223328932E-4
56-57	0.0	0.0018169579279065703	0.0	0.060103814631068134	3.316669233480247E-4
58-59	0.0	0.0018169579279065703	0.0	0.06649921813127896	3.460872243631562E-4
60-61	0.0	0.0018313782289217017	0.0	0.07250527350408123	3.460872243631562E-4
62-63	0.0	0.0018746391319670963	0.0	0.0788285754992164	3.8934812740855075E-4
64-65	0.0	0.0020476827441486744	0.0	0.08433713048699663	3.8934812740855075E-4
66-67	0.0	0.002083733496686503	0.0	0.08993941743137523	3.8934812740855075E-4
68-69	0.0	0.002148624851254595	0.0	0.09561380588082949	3.9655827791611653E-4
70-71	0.0	0.0021846756037924237	0.0	0.10045902702191367	4.181887294388138E-4
72-73	0.0	0.002199095904807555	0.0	0.10481395792848339	4.3260903045394533E-4
74-75	0.0	0.0022423568078529495	0.0	0.10802247490435014	4.3260903045394533E-4
76-77	0.0	0.002357719215974002	0.0	0.1115049775995044	4.398191809615111E-4
78-79	0.0	0.002379349667496699	0.0	0.1136752329022817	4.4702933146907684E-4
80-81	0.0	0.002379349667496699	0.0	0.11451161036115931	4.5423948197664257E-4
82-83	0.0	0.002386559818004265	0.0	0.11505958179973433	5.047105355296029E-4
84-85	0.0	0.0024009801190193964	0.0	0.11531914721800668	5.047105355296029E-4
86-87	0.0	0.002451451172572357	0.0	0.11544171977663531	5.191308365447343E-4
88	0.0	0.0026100744837388033	0.0	0.115463350228158	5.335511375598659E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8160	0.0	51.885532	1
GTATCAA	14500	0.0	35.769547	1
TCAACGC	16720	0.0	29.997078	4
ATCAACG	16920	0.0	29.668394	3
CAACGCA	17130	0.0	29.279106	5
AACGCAG	17595	0.0	28.675285	6
TATCAAC	17930	0.0	28.259676	2
ACGCAGA	20505	0.0	24.45959	7
CGCAGAG	21220	0.0	23.615301	8
GTACATG	20190	0.0	20.652674	1
TACATGG	20070	0.0	20.137547	2
GCAGAGT	25165	0.0	20.032074	9
GAGTACT	14105	0.0	19.702213	12-13
ACATGGG	20215	0.0	19.527863	3
CAGAGTA	24430	0.0	18.772383	10-11
CATGGGG	14770	0.0	18.077843	4
AGAGTAC	22535	0.0	17.611607	10-11
GTGGTAT	4235	0.0	17.351532	1
GTACTTT	16390	0.0	17.033823	14-15
TGGTATC	3835	0.0	16.82187	2
>>END_MODULE