FastQCFastQC Report
Fri 27 May 2016
SRR2139171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041210
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72100.050.1213151
GTATCAA131600.036.189981
TCAACGC150800.030.7565844
ATCAACG152100.030.4660323
CAACGCA153100.030.2385375
AACGCAG158600.029.2439676
TATCAAC160650.029.1388282
ACGCAGA187000.024.7105377
CGCAGAG193450.023.8644838
GTACATG170000.020.626931
GCAGAGT227600.020.3402619
TACATGG170400.020.125682
ACATGGG166750.019.9487323
TATACCG3450.019.879855
GAGTACT135500.019.05999612-13
CAGAGTA220800.018.60793110-11
AGAGTAC204900.017.85527610-11
GTGGTAT35350.017.583641
CATGGGG118700.016.7563534
GTACTTT156750.016.62657514-15
Produced by FastQC (version 0.11.5)