##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6041210
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.83700202442888	28.0	18.0	32.0	18.0	33.0
2	27.888805884913783	29.0	25.0	32.0	18.0	33.0
3	30.688679089122875	31.0	29.0	33.0	27.0	33.0
4	30.230532956146202	31.0	29.0	33.0	27.0	33.0
5	31.78309643266829	33.0	31.0	33.0	29.0	33.0
6	36.17033607505781	38.0	36.0	38.0	33.0	38.0
7	36.51577763395081	38.0	37.0	38.0	34.0	38.0
8	36.70911390267844	38.0	37.0	38.0	34.0	38.0
9	36.990608503925536	38.0	38.0	38.0	35.0	38.0
10-11	37.153496650505446	38.0	38.0	38.0	36.0	38.0
12-13	37.253635198908825	38.0	38.0	38.0	36.0	38.0
14-15	37.163825127747586	38.0	38.0	38.0	36.0	38.0
16-17	37.349124099311226	38.0	38.0	38.0	36.5	38.0
18-19	37.38078199565981	38.0	38.0	38.0	37.0	38.0
20-21	37.41569018084159	38.0	38.0	38.0	37.0	38.0
22-23	37.435546863018764	38.0	38.0	38.0	37.0	38.0
24-25	37.45049754218982	38.0	38.0	38.0	37.0	38.0
26-27	37.41252486135495	38.0	38.0	38.0	37.0	38.0
28-29	37.38799928390853	38.0	38.0	38.0	37.0	38.0
30-31	37.3927022710077	38.0	38.0	38.0	37.0	38.0
32-33	37.383744286529854	38.0	38.0	38.0	37.0	38.0
34-35	37.35530904562197	38.0	38.0	38.0	37.0	38.0
36-37	37.332822113217844	38.0	38.0	38.0	37.0	38.0
38-39	37.33898349439985	38.0	38.0	38.0	37.0	38.0
40-41	37.33503406962048	38.0	38.0	38.0	37.0	38.0
42-43	37.29572132282424	38.0	38.0	38.0	37.0	38.0
44-45	37.20851532618181	38.0	38.0	38.0	37.0	38.0
46-47	37.24449904426125	38.0	38.0	38.0	37.0	38.0
48-49	37.19988502158785	38.0	38.0	38.0	37.0	38.0
50-51	37.15744105781711	38.0	38.0	38.0	37.0	38.0
52-53	37.142397960347004	38.0	38.0	38.0	36.0	38.0
54-55	37.12214358907535	38.0	38.0	38.0	36.0	38.0
56-57	37.08496762966007	38.0	38.0	38.0	36.0	38.0
58-59	37.07557420100002	38.0	38.0	38.0	36.0	38.0
60-61	37.08721038088315	38.0	38.0	38.0	36.0	38.0
62-63	37.09758286109009	38.0	38.0	38.0	36.0	38.0
64-65	37.06179455618988	38.0	38.0	38.0	36.0	38.0
66-67	37.09321845525435	38.0	38.0	38.0	36.0	38.0
68-69	37.084037170443224	38.0	38.0	38.0	36.0	38.0
70-71	37.09025757453697	38.0	38.0	38.0	36.0	38.0
72-73	37.0782621572611	38.0	38.0	38.0	36.0	38.0
74-75	37.07841715985076	38.0	38.0	38.0	36.0	38.0
76-77	37.069172922591335	38.0	38.0	38.0	36.0	38.0
78-79	37.050577837685736	38.0	38.0	38.0	36.0	38.0
80-81	37.02500362046983	38.0	38.0	38.0	36.0	38.0
82-83	36.99545789978265	38.0	38.0	38.0	36.0	38.0
84-85	36.977792086165614	38.0	38.0	38.0	36.0	38.0
86-87	36.974266662111944	38.0	38.0	38.0	36.0	38.0
88-89	36.96151874821	38.0	38.0	38.0	36.0	38.0
90-91	36.94271464131974	38.0	38.0	38.0	36.0	38.0
92-93	36.92391733563619	38.0	38.0	38.0	35.0	38.0
94-95	36.89973982879012	38.0	38.0	38.0	35.0	38.0
96-97	36.904564563433084	38.0	38.0	38.0	35.0	38.0
98-99	36.910299651435146	38.0	38.0	38.0	35.0	38.0
100	35.592939419417796	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	25.0
21	88.0
22	365.0
23	1106.0
24	2706.0
25	5621.0
26	10028.0
27	16905.0
28	25986.0
29	37377.0
30	50343.0
31	65316.0
32	85971.0
33	117097.0
34	182841.0
35	355604.0
36	1128797.0
37	3955026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4189872558643	21.457456370495315	13.527935628789598	23.595620744850784
2	15.013587754947318	21.257995852080604	41.987213920111586	21.741202472860493
3	17.83420396027246	27.65370716244811	30.49869319053694	24.013395686742484
4	12.531746542786387	18.382992543379974	39.79806028892561	29.287200624908028
5	13.094164910671868	38.0093722946231	36.14189872558643	12.754564069118604
6	28.98370463852431	39.03108455700695	19.243301832245326	12.741908972223412
7	25.56037614981105	33.31372357524403	24.351595127466187	16.77430514747873
8	23.590356898700758	37.897739029101785	21.60017612365735	16.911727948540108
9	25.606856904494297	17.05539122129507	21.852244831747285	35.48550704246335
10-11	23.825504133361385	27.051274008232458	29.67414303991138	19.449078818494776
12-13	24.715942667114703	24.862693069765825	29.650136313751712	20.77122794936776
14-15	21.945413253305215	26.113882814866557	27.191845673300545	24.74885825852768
16-17	21.39836224862238	29.069044115334506	27.913514014576553	21.61907962146656
18-19	21.380791265325986	28.348468270429265	29.571898675927503	20.69884178831724
20-21	22.23829711089833	27.558352328692703	29.447506719933404	20.755843840475563
22-23	21.983232607919437	27.464939229542868	29.22692079190056	21.324907370637135
24-25	21.73360770771166	27.615771245866444	29.295921143605124	21.354699902816773
26-27	21.647956916470694	27.747505458620115	29.27193729523966	21.33260032966953
28-29	21.636287065454066	27.785187795650877	29.28621323760775	21.2923119012873
30-31	21.919445478058876	27.60642798598778	29.20500455388879	21.269121982064558
32-33	21.712896251359343	27.784158754002476	29.1178847145451	21.38506028009308
34-35	21.84795894075454	27.803158791377996	29.109754233479567	21.239128034387893
36-37	21.849821632882332	27.714220878670954	29.089285750066956	21.346671738379754
38-39	21.691003374293693	27.86389747410039	29.161045011532245	21.284054140073675
40-41	21.93416275423229	27.692069601418584	29.062505959574636	21.31126168477449
42-43	21.870644077371033	27.791830266605377	28.855396778085495	21.482128877938095
44-45	21.889008940769834	27.994963062113687	28.68656785800593	21.429460139110546
46-47	21.960146835337056	27.89446750656167	28.492212433180608	21.65317322492066
48-49	21.97868346331338	27.997287961827254	28.45202928369147	21.571999291167895
50-51	21.918871828035556	28.168141323495753	28.32718134779575	21.58580550067294
52-53	22.078121831408886	28.055532700344816	28.243038415371014	21.62330705287529
54-55	22.033990623056155	28.024451163495424	28.219115183545178	21.722443029903246
56-57	21.996047828519224	28.139496717479663	28.234486564271528	21.629968889729586
58-59	22.00358438324224	28.11966148012628	28.248723805181537	21.628030331449942
60-61	22.068152740312836	28.139177940237385	28.280037541956315	21.51263177749346
62-63	22.034378497624534	28.22118539032471	28.22876057124742	21.515675540803343
64-65	22.043114531530737	28.132462147322308	28.208159656015752	21.616263665131207
66-67	22.08788466999027	28.290810745390477	28.047675571506375	21.57362901311288
68-69	22.059954899809767	28.153985874694825	28.185382957509376	21.60067626798603
70-71	22.075983559506604	27.914082765201393	28.23899692544452	21.770936749847486
72-73	22.157050098828577	28.063745700207953	28.11414822649819	21.66505597446527
74-75	21.948125932978133	28.1304518269474	28.16206445732295	21.759357782751515
76-77	22.062623445692665	28.01048661110575	28.098095903478743	21.828794039722837
78-79	22.003952778786566	27.954845794497814	28.100051921177023	21.941149505538604
80-81	22.038365990915988	28.024818503632908	28.04913399665786	21.887681508793243
82-83	22.090446013945495	27.953583191792948	28.200440071057493	21.755530723204064
84-85	22.185448763235836	27.92694193760615	28.060602047481026	21.82700725167699
86-87	22.021479897292128	27.99052547718549	28.128592401058523	21.85940222446386
88-89	22.109015636521086	27.940382222394994	28.050291941552967	21.90031019953096
90-91	22.17096543777165	27.992289086731788	28.07065627399342	21.766089201503146
92-93	22.195155010117016	27.920837014592735	28.062674887333127	21.82133308795712
94-95	22.05002169867622	27.971121423057095	28.085309237653178	21.893547640613512
96-97	22.126844390197746	28.170035479309234	28.124948456472087	21.578171674020933
98-99	23.19687335370567	30.08817433884468	28.31070631859694	18.40424598885271
100	29.384134877201166	41.0091805044854	29.606684618313434	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1021.0
1	965.5
2	1264.0
3	1766.5
4	2384.0
5	3509.0
6	4380.0
7	5146.0
8	5968.0
9	6414.0
10	6408.5
11	6397.5
12	7207.5
13	9164.0
14	12956.0
15	17406.0
16	20761.5
17	22809.0
18	23359.5
19	22567.5
20	21344.5
21	20727.5
22	21933.5
23	25886.0
24	32616.0
25	42389.0
26	54875.0
27	68814.0
28	83631.0
29	99247.0
30	113851.5
31	128324.5
32	144468.5
33	160839.0
34	174938.0
35	187972.5
36	202047.5
37	214437.0
38	223940.0
39	230255.5
40	235574.5
41	239933.0
42	242169.5
43	244591.5
44	247859.0
45	249791.0
46	249082.5
47	248124.0
48	245213.0
49	239413.0
50	232559.5
51	224725.0
52	214173.5
53	200984.0
54	185659.0
55	169331.0
56	152834.0
57	135741.5
58	118741.5
59	100539.5
60	83036.5
61	66897.5
62	52189.0
63	39998.0
64	29625.0
65	21633.5
66	15773.5
67	11591.5
68	8556.0
69	6249.0
70	4532.0
71	3117.0
72	2161.5
73	1470.5
74	969.0
75	644.5
76	407.5
77	244.0
78	153.5
79	102.0
80	71.0
81	41.5
82	24.0
83	15.0
84	8.5
85	6.0
86	4.0
87	1.5
88	0.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018042743092857227
3	0.002665029025642214
4	1.655297531454791E-5
5	0.0
6	7.117779385255604E-4
7	0.0
8	0.0
9	0.0
10-11	1.655297531454791E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.9864185254715966E-4
24-25	0.0012415725161534791
26-27	0.0028393289945406242
28-29	0.0018216498782309858
30-31	0.002693310273072657
32-33	0.0035033030253346087
34-35	0.001164910467061699
36-37	8.348956317843098E-6
38-39	8.391044841995368E-5
40-41	0.0
42-43	5.855955213654526E-4
44-45	0.0
46-47	0.0
48-49	2.2574219910930808E-4
50-51	0.006867145920552283
52-53	0.0
54-55	0.002989419141667686
56-57	0.04995180473242397
58-59	0.06255318845975233
60-61	0.040852223170534135
62-63	0.009223099917770615
64-65	0.0
66-67	0.0
68-69	6.482300032787286E-4
70-71	0.0031104001444439483
72-73	0.011072781189785172
74-75	0.005223439125837669
76-77	0.0044041437146146055
78-79	0.004781596638084984
80-81	0.017393586040603586
82-83	0.05794873524777
84-85	0.03812336156212324
86-87	0.010397435844955403
88-89	0.00909714552248835
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	29.0
20-21	105.0
22-23	265.0
24-25	453.0
26-27	1206.0
28-29	3322.0
30-31	10012.0
32-33	12801.0
34-35	17784.0
36-37	28048.0
38-39	33721.0
40-41	33262.0
42-43	38070.0
44-45	45407.0
46-47	46692.0
48-49	44130.0
50-51	40603.0
52-53	38454.0
54-55	42201.0
56-57	45240.0
58-59	45388.0
60-61	44240.0
62-63	42545.0
64-65	44677.0
66-67	48197.0
68-69	49253.0
70-71	49063.0
72-73	46163.0
74-75	46267.0
76-77	48791.0
78-79	51325.0
80-81	51004.0
82-83	48158.0
84-85	46573.0
86-87	48454.0
88-89	50983.0
90-91	52388.0
92-93	52246.0
94-95	59356.0
96-97	183591.0
98-99	1398841.0
100-101	3051902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.19865012301679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07635548971324	77.64460251214274
2	7.075002010403321	12.197112458287926
3	1.6573683414458555	4.285887413677679
4	0.5981035705122836	2.0622288164766176
5	0.24344481107498264	1.0492307047058176
6	0.13373330833162195	0.6916578392802605
7	0.06661823242985278	0.4019681196324283
8	0.04111924623447342	0.28355388155900396
9	0.028667821959540707	0.22240147993914483
>10	0.07816804256449439	1.0065800732018153
>50	8.305064126854984E-4	0.046441535361937375
>100	5.88618917669798E-4	0.10833516573464884
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6552975314547915E-5
2	0.0	4.965892594364374E-5	0.0	0.0	3.310595062909583E-5
3	0.0	4.965892594364374E-5	0.0	0.0	3.310595062909583E-5
4	0.0	4.965892594364374E-5	0.0	0.0	4.965892594364374E-5
5	0.0	6.621190125819166E-5	0.0	0.0	4.965892594364374E-5
6	0.0	6.621190125819166E-5	0.0	0.0	4.965892594364374E-5
7	0.0	6.621190125819166E-5	0.0	0.0	4.965892594364374E-5
8	0.0	8.276487657273956E-5	0.0	1.6552975314547915E-5	4.965892594364374E-5
9	0.0	1.6552975314547912E-4	0.0	4.965892594364374E-5	4.965892594364374E-5
10-11	0.0	1.6552975314547912E-4	0.0	4.965892594364374E-5	4.965892594364374E-5
12-13	0.0	1.6552975314547912E-4	0.0	4.965892594364374E-5	4.965892594364374E-5
14-15	0.0	2.3174165440367078E-4	0.0	7.448838891546561E-5	7.448838891546561E-5
16-17	0.0	2.3174165440367078E-4	0.0	1.0759433954456144E-4	8.276487657273956E-5
18-19	0.0	2.896770680045885E-4	0.0	1.3242380251638332E-4	1.1587082720183539E-4
20-21	0.0	3.476124816055062E-4	0.0	1.4897677783093122E-4	1.1587082720183539E-4
22-23	0.0	3.889949198918759E-4	0.0	2.1518867908912288E-4	1.1587082720183539E-4
24-25	0.0	4.386538458355197E-4	0.0	2.2346516674639683E-4	1.1587082720183539E-4
26-27	0.0	4.5520682115006764E-4	0.0	2.7312409269004056E-4	1.3242380251638332E-4
28-29	0.0	4.6348330880734156E-4	0.0	4.4693033349279366E-4	1.3242380251638332E-4
30-31	0.0	5.628011606946291E-4	0.0	9.766255435583269E-4	1.3242380251638332E-4
32-33	0.0	5.793541360091769E-4	0.0	0.0026153700996985703	1.4897677783093122E-4
34-35	0.0	6.372895496100947E-4	0.0	0.004676215526359786	1.4897677783093122E-4
36-37	0.0	6.952249632110124E-4	0.0	0.007407456453260191	1.6552975314547912E-4
38-39	0.0	7.035014508682863E-4	0.0	0.011702953547385375	1.6552975314547912E-4
40-41	0.0	7.61436864469204E-4	0.0	0.016577804777519734	1.6552975314547912E-4
42-43	0.0	7.779898397837519E-4	0.0	0.02111332001370586	1.6552975314547912E-4
44-45	0.0	9.021371546428614E-4	0.0	0.026137148021671157	1.6552975314547912E-4
46-47	0.0	0.0010097314941874228	0.0	0.03215415454850932	1.6552975314547912E-4
48-49	0.0	0.0010097314941874228	0.0	0.037186259044131885	1.6552975314547912E-4
50-51	0.0	0.001133878809046532	0.0	0.04232595787929901	1.6552975314547912E-4
52-53	0.0	0.001142155296703806	0.0	0.04771395134418436	1.6552975314547912E-4
54-55	0.0	0.001142155296703806	0.0	0.053358515926445194	1.6552975314547912E-4
56-57	0.0	0.001175261247332902	0.0	0.058986527533391495	1.6552975314547912E-4
58-59	0.0	0.0011918142226474498	0.0	0.06464764509096688	1.6552975314547912E-4
60-61	0.0	0.0011918142226474498	0.0	0.07081362839563599	1.6552975314547912E-4
62-63	0.0	0.0012414731485910935	0.0	0.07662372273104229	1.6552975314547912E-4
64-65	0.0	0.0014235558770511205	0.0	0.08224345785033131	1.6552975314547912E-4
66-67	0.0	0.0014401088523656686	0.0	0.08779698106836213	1.6552975314547912E-4
68-69	0.0	0.0014483853400229425	0.0	0.09373122271862755	1.6552975314547912E-4
70-71	0.0	0.0014566618276802164	0.0	0.09878815667722195	1.8208272846002705E-4
72-73	0.0	0.0014566618276802164	0.0	0.10282708265397164	1.8208272846002705E-4
74-75	0.0	0.0014649383153374903	0.0	0.10616250717985304	1.8208272846002705E-4
76-77	0.0	0.0014814912906520382	0.0	0.10917514868710076	1.8208272846002705E-4
78-79	0.0	0.0014897677783093123	0.0	0.11153394766942384	1.8208272846002705E-4
80-81	0.0	0.0014980442659665862	0.0	0.11219606668200575	1.8208272846002705E-4
82-83	0.0	0.001514597241281134	0.0	0.11274231486738584	1.8208272846002705E-4
84-85	0.0	0.001531150216595682	0.0	0.11295750354647496	1.9863570377457495E-4
86-87	0.0	0.0015890856301965996	0.0	0.11308165086133407	1.9863570377457495E-4
88	0.0	0.001704956457398435	0.0	0.11312303329962044	1.9863570377457495E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7210	0.0	50.121315	1
GTATCAA	13160	0.0	36.18998	1
TCAACGC	15080	0.0	30.756584	4
ATCAACG	15210	0.0	30.466032	3
CAACGCA	15310	0.0	30.238537	5
AACGCAG	15860	0.0	29.243967	6
TATCAAC	16065	0.0	29.138828	2
ACGCAGA	18700	0.0	24.710537	7
CGCAGAG	19345	0.0	23.864483	8
GTACATG	17000	0.0	20.62693	1
GCAGAGT	22760	0.0	20.340261	9
TACATGG	17040	0.0	20.12568	2
ACATGGG	16675	0.0	19.948732	3
TATACCG	345	0.0	19.87985	5
GAGTACT	13550	0.0	19.059996	12-13
CAGAGTA	22080	0.0	18.607931	10-11
AGAGTAC	20490	0.0	17.855276	10-11
GTGGTAT	3535	0.0	17.58364	1
CATGGGG	11870	0.0	16.756353	4
GTACTTT	15675	0.0	16.626575	14-15
>>END_MODULE