FastQCFastQC Report
Fri 27 May 2016
SRR2139170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6250802
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77300.052.993521
GTATCAA144800.037.513781
TCAACGC173500.030.8398084
ATCAACG176050.030.4178313
CAACGCA177950.030.0925755
TATCAAC183050.029.3951932
AACGCAG184550.029.0163846
ACGCAGA211750.025.2284777
CGCAGAG217800.024.4297318
GTACATG211400.023.9594421
TACATGG214850.023.1774922
ACATGGG214300.022.738643
GCAGAGT254900.021.0414459
TGGTATC36950.020.0939732
CATGGGG141100.019.898144
GTGGTAT38950.019.7195051
GAGTACT142100.019.35258312-13
CAGAGTA249950.018.77801510-11
AGAGTAC231750.018.30108310-11
GTACTTT166200.016.49506414-15
Produced by FastQC (version 0.11.5)