##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6250802
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.800562871772293	28.0	18.0	32.0	18.0	33.0
2	27.699631023347084	29.0	25.0	31.0	18.0	33.0
3	30.60123868905142	31.0	29.0	33.0	27.0	33.0
4	30.06586370836894	31.0	29.0	33.0	25.0	33.0
5	31.71050594787677	33.0	31.0	33.0	29.0	33.0
6	36.09311925093772	38.0	36.0	38.0	33.0	38.0
7	36.423086189580154	38.0	37.0	38.0	34.0	38.0
8	36.65996187369237	38.0	37.0	38.0	34.0	38.0
9	36.960342368867224	38.0	38.0	38.0	35.0	38.0
10-11	37.133857703379505	38.0	38.0	38.0	36.0	38.0
12-13	37.23675705933415	38.0	38.0	38.0	36.0	38.0
14-15	37.15033718873194	38.0	38.0	38.0	36.0	38.0
16-17	37.34520586318364	38.0	38.0	38.0	36.5	38.0
18-19	37.37748436120677	38.0	38.0	38.0	37.0	38.0
20-21	37.41427930754654	38.0	38.0	38.0	37.0	38.0
22-23	37.43628253359033	38.0	38.0	38.0	37.0	38.0
24-25	37.45310385536112	38.0	38.0	38.0	37.0	38.0
26-27	37.413012715364374	38.0	38.0	38.0	37.0	38.0
28-29	37.38958947791147	38.0	38.0	38.0	37.0	38.0
30-31	37.399548674760474	38.0	38.0	38.0	37.0	38.0
32-33	37.38764255852827	38.0	38.0	38.0	37.0	38.0
34-35	37.35805219732394	38.0	38.0	38.0	37.0	38.0
36-37	37.335605013698334	38.0	38.0	38.0	37.0	38.0
38-39	37.346216757876945	38.0	38.0	38.0	37.0	38.0
40-41	37.3419969664741	38.0	38.0	38.0	37.0	38.0
42-43	37.30154844291456	38.0	38.0	38.0	37.0	38.0
44-45	37.21616781455472	38.0	38.0	38.0	37.0	38.0
46-47	37.24771569108037	38.0	38.0	38.0	37.0	38.0
48-49	37.20239220622875	38.0	38.0	38.0	37.0	38.0
50-51	37.163260684121084	38.0	38.0	38.0	37.0	38.0
52-53	37.14554708441193	38.0	38.0	38.0	36.5	38.0
54-55	37.13020220879949	38.0	38.0	38.0	36.0	38.0
56-57	37.09826163904883	38.0	38.0	38.0	36.0	38.0
58-59	37.09200721490792	38.0	38.0	38.0	36.0	38.0
60-61	37.10504402562877	38.0	38.0	38.0	36.0	38.0
62-63	37.11767833127311	38.0	38.0	38.0	36.0	38.0
64-65	37.086388068126936	38.0	38.0	38.0	36.0	38.0
66-67	37.1209799433591	38.0	38.0	38.0	36.0	38.0
68-69	37.11487664306789	38.0	38.0	38.0	36.0	38.0
70-71	37.12267507580243	38.0	38.0	38.0	36.0	38.0
72-73	37.11218558883334	38.0	38.0	38.0	36.0	38.0
74-75	37.11365213239668	38.0	38.0	38.0	36.0	38.0
76-77	37.105343079853	38.0	38.0	38.0	36.0	38.0
78-79	37.08967743752552	38.0	38.0	38.0	36.0	38.0
80-81	37.064389888531636	38.0	38.0	38.0	36.0	38.0
82-83	37.03804838954078	38.0	38.0	38.0	36.0	38.0
84-85	37.0214638128349	38.0	38.0	38.0	36.0	38.0
86-87	37.01968354726042	38.0	38.0	38.0	36.0	38.0
88-89	37.00872794041406	38.0	38.0	38.0	36.0	38.0
90-91	36.99227073675671	38.0	38.0	38.0	36.0	38.0
92-93	36.97584075640148	38.0	38.0	38.0	36.0	38.0
94-95	36.95186034057433	38.0	38.0	38.0	35.5	38.0
96-97	36.95554886598999	38.0	38.0	38.0	35.0	38.0
98-99	36.959647342010356	38.0	38.0	38.0	35.0	38.0
100	35.66472971118875	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	26.0
21	85.0
22	343.0
23	1032.0
24	2668.0
25	5389.0
26	9651.0
27	16276.0
28	25506.0
29	37478.0
30	50691.0
31	67182.0
32	87504.0
33	121176.0
34	191037.0
35	376476.0
36	1196620.0
37	4061652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89785566716079	21.13330417440834	13.462032551982931	23.50680760644794
2	15.250463869382571	21.10585692889192	41.82065253039588	21.82302667132962
3	17.959990644155045	27.634411079317022	30.318265111497418	24.087333165030515
4	12.587858740023878	18.20118733583104	39.41427666630244	29.796677257842635
5	13.181380565245867	38.001859601375955	35.845080359288296	12.971679474089884
6	29.173705792688782	38.60746249745632	19.209198985853916	13.009632724000989
7	25.87044030510005	33.06934054222162	24.23103467363068	16.829184479047647
8	23.912035607590834	37.48322215293334	21.603739808107825	17.001002431368008
9	26.026804240479862	16.783654321477467	21.590333528401636	35.59920790964103
10-11	23.999370320750067	26.88195265839109	29.500824814225822	19.61785220663302
12-13	24.919770295075736	24.71501256958707	29.489647888382965	20.87556924695423
14-15	22.214069490602967	25.831229336651525	27.02004478785282	24.93465638489269
16-17	21.55516204160682	28.883333690620823	27.780763172469708	21.78074109530265
18-19	21.62607294232004	28.06393483588186	29.433791055931703	20.876201165866394
20-21	22.518660509982215	27.239873122770376	29.294852935647736	20.946613431599673
22-23	22.290702446961934	27.125080301924836	29.10158554455764	21.482631706555587
24-25	21.95689866059079	27.31442349982633	29.213398475879448	21.515279363703428
26-27	21.858062346206793	27.41375079774257	29.222799328572176	21.50538752747847
28-29	21.858208310836048	27.34280088006107	29.284981991366642	21.51400881773624
30-31	22.062583048528776	27.30266388042774	29.141549534367574	21.49320353667591
32-33	21.862309908665644	27.487072340887863	29.081842716309346	21.56877503413714
34-35	22.005425738888746	27.46327282776806	29.068932171507655	21.462369261835534
36-37	22.01930483263304	27.370061003284036	28.995346238574747	21.615287925508177
38-39	21.85555663193949	27.535707684845818	29.140190461539913	21.46854522167478
40-41	22.082554598740938	27.406283769987795	29.006947528508174	21.504214102763097
42-43	21.98273859335363	27.471874444347577	28.804668604995754	21.74071835730304
44-45	22.014948479988732	27.574167162670154	28.69637385139606	21.714510505945057
46-47	22.131086925764887	27.57355684468355	28.450442156858742	21.844914072692823
48-49	22.066772697776617	27.6874359355679	28.38139354940892	21.864397817246566
50-51	22.045294385029727	27.786715124940386	28.31088036711142	21.857110122918467
52-53	22.28400655070759	27.651455236191254	28.18555899328753	21.87897921981363
54-55	22.228650898675077	27.618198879782106	28.212474024917505	21.94067619662531
56-57	22.130376425118996	27.751288895103766	28.21040125532003	21.90793342445721
58-59	22.126814721519615	27.70519120205137	28.24126002427082	21.926734052158196
60-61	22.199871124920556	27.74381223077466	28.254457665419107	21.801858978885672
62-63	22.190609119207696	27.742425065136562	28.274760127433552	21.79220568822219
64-65	22.186609942361248	27.66458929697386	28.27086083250866	21.877939928156234
66-67	22.26187920083276	27.75965551699177	28.111304685001524	21.867160597173942
68-69	22.218801494844648	27.690252393871273	28.238445049765787	21.85250106151829
70-71	22.25697841713319	27.43515642367474	28.36329503024208	21.94457012894999
72-73	22.34285756434605	27.632283259966478	28.05336268318878	21.97149649249869
74-75	22.100148294043535	27.77563553277225	28.104629068365977	22.019587104818243
76-77	22.26557811032699	27.601252541040992	28.049370564333614	22.0837987842984
78-79	22.176434156671952	27.593618620655285	28.033176353925743	22.196770868747016
80-81	22.24221108098678	27.716629378447657	27.90219991251591	22.13895962804965
82-83	22.30659005495647	27.616383149830348	28.01671395881727	22.060312836395912
84-85	22.308765895477613	27.618559411511857	27.965557720141028	22.107116972869505
86-87	22.17768721549418	27.69234475455335	28.037551004020095	22.092417025932377
88-89	22.310361158899827	27.637472944110087	27.956486101931382	22.095679795058707
90-91	22.327573100324717	27.715589380623275	27.917969009451603	22.038868509600405
92-93	22.359186769437343	27.701991774786507	27.864081935932422	22.074739519843735
94-95	22.266242590815796	27.614102330377545	27.977714570121705	22.141940508684954
96-97	22.397848924633287	27.729449891305	27.947299380143566	21.92540180391815
98-99	23.41401781921673	29.68094280363539	28.21042872722963	18.694610649918246
100	29.837720648313	40.626016658357706	29.536262693329295	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1121.0
1	1029.0
2	1456.0
3	2170.5
4	2781.0
5	3990.0
6	5075.5
7	6254.5
8	7537.5
9	8141.5
10	7894.5
11	7596.5
12	8491.0
13	10481.5
14	14170.0
15	18684.0
16	22185.0
17	24188.0
18	24262.0
19	22918.5
20	21235.0
21	20275.0
22	21211.0
23	24632.0
24	30884.5
25	40196.0
26	52094.5
27	65704.0
28	79709.0
29	94959.0
30	110084.0
31	124698.0
32	141700.5
33	159513.0
34	174332.5
35	189255.0
36	206081.5
37	218511.0
38	228578.0
39	236373.0
40	242853.5
41	248672.0
42	250532.0
43	253760.5
44	257923.5
45	258598.5
46	256528.5
47	254039.5
48	252226.0
49	249257.5
50	244618.0
51	238407.0
52	229419.5
53	216461.0
54	201073.0
55	186160.0
56	168897.0
57	150497.5
58	132627.0
59	113450.5
60	95123.5
61	77349.0
62	60757.5
63	46650.0
64	34454.0
65	25070.0
66	18252.5
67	13616.0
68	10261.0
69	7510.5
70	5394.5
71	3760.0
72	2572.5
73	1696.0
74	1130.5
75	726.0
76	448.0
77	273.5
78	179.0
79	108.0
80	60.5
81	41.5
82	30.5
83	17.5
84	9.0
85	4.0
86	3.0
87	2.0
88	1.5
89	2.5
90	3.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019197536572107068
3	0.002623663331521299
4	1.5997947143422556E-5
5	0.0
6	4.1594662572898647E-4
7	0.0
8	0.0
9	0.0
10-11	7.998973571711278E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.6798282543592743E-4
24-25	0.001167931605925157
26-27	0.002784071049493183
28-29	0.001712525402793577
30-31	0.0026510750349545446
32-33	0.003281808143088761
34-35	0.0012547135203505283
36-37	0.0
38-39	4.056721732337013E-5
40-41	1.63213930374243E-5
42-43	6.403045091228617E-4
44-45	0.0
46-47	0.0
48-49	2.437941771862453E-4
50-51	0.006100977274876481
52-53	0.0
54-55	0.0029839710876419824
56-57	0.04762693362489259
58-59	0.059211131062920266
60-61	0.03775672965837439
62-63	0.008509088436309029
64-65	0.0
66-67	0.0
68-69	6.312803243280433E-4
70-71	0.0026897558015999148
72-73	0.010334746837912892
74-75	0.004853983687975749
76-77	0.0037789937824605573
78-79	0.003953669914736367
80-81	0.016241349171793307
82-83	0.05532715572411921
84-85	0.03603620068786631
86-87	0.009866077284807356
88-89	0.008523610248180134
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	22.0
20-21	90.0
22-23	252.0
24-25	390.0
26-27	1292.0
28-29	3427.0
30-31	10879.0
32-33	13700.0
34-35	19049.0
36-37	30117.0
38-39	35947.0
40-41	35289.0
42-43	40851.0
44-45	49351.0
46-47	50344.0
48-49	47792.0
50-51	44268.0
52-53	41995.0
54-55	46733.0
56-57	49489.0
58-59	50430.0
60-61	49687.0
62-63	47431.0
64-65	49832.0
66-67	53324.0
68-69	55509.0
70-71	54751.0
72-73	50927.0
74-75	51532.0
76-77	54329.0
78-79	56527.0
80-81	55800.0
82-83	52775.0
84-85	51757.0
86-87	53589.0
88-89	56379.0
90-91	57544.0
92-93	56709.0
94-95	63735.0
96-97	189914.0
98-99	1434027.0
100-101	3083017.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.17020731770272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.90473513868433	79.24184608199968
2	6.562905520431368	11.44179669644996
3	1.4799111870844557	3.870124949698185
4	0.49852137657944506	1.738248469949471
5	0.2270242086941461	0.9894873669003061
6	0.11628562177572442	0.6081985054954718
7	0.06258176132171912	0.38186855761048716
8	0.0410164888871976	0.28603326717930116
9	0.025197469477259885	0.19768217743928074
>10	0.07967277234881913	1.069201757540214
>50	0.001868543919033606	0.10828862577385749
>100	2.524490815167341E-4	0.05214233061472823
>500	2.7461714987262508E-5	0.015081213349057822
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5997947143422556E-5	0.0	0.0	0.0
3	0.0	1.5997947143422556E-5	0.0	0.0	0.0
4	0.0	7.998973571711278E-5	0.0	0.0	0.0
5	0.0	7.998973571711278E-5	0.0	0.0	0.0
6	0.0	7.998973571711278E-5	0.0	0.0	0.0
7	0.0	7.998973571711278E-5	0.0	0.0	0.0
8	0.0	1.1198563000395789E-4	0.0	0.0	0.0
9	0.0	2.87963048581606E-4	0.0	1.5997947143422556E-5	0.0
10-11	0.0	2.87963048581606E-4	0.0	1.5997947143422556E-5	0.0
12-13	0.0	2.87963048581606E-4	0.0	1.5997947143422556E-5	0.0
14-15	0.0	3.5195483715529623E-4	0.0	1.5997947143422556E-5	0.0
16-17	0.0	3.5195483715529623E-4	0.0	1.5997947143422556E-5	0.0
18-19	0.0	4.399435464441203E-4	0.0	1.5997947143422556E-5	1.5997947143422556E-5
20-21	0.0	5.119343085895218E-4	0.0	1.5997947143422556E-5	1.5997947143422556E-5
22-23	0.0	5.679271235915007E-4	0.0	3.199589428684511E-5	1.5997947143422556E-5
24-25	0.0	6.079219914500571E-4	0.0	4.7993841430267665E-5	2.3996920715133833E-5
26-27	0.0	6.239199385934796E-4	0.0	5.5992815001978945E-5	3.199589428684511E-5
28-29	0.0	6.239199385934796E-4	0.0	2.3996920715133833E-4	3.199589428684511E-5
30-31	0.0	7.679014628842826E-4	0.0	0.001151852194326424	3.199589428684511E-5
32-33	0.0	7.918983835994165E-4	0.0	0.0033195740322601803	3.199589428684511E-5
34-35	0.0	9.118829871750857E-4	0.0	0.006359183989510466	3.199589428684511E-5
36-37	7.998973571711278E-6	0.0010238686171790436	0.0	0.010374668722509528	3.199589428684511E-5
38-39	1.5997947143422556E-5	0.0010238686171790436	0.0	0.017229789073466093	3.199589428684511E-5
40-41	1.5997947143422556E-5	0.0011038583528961564	0.0	0.02527675648660764	3.199589428684511E-5
42-43	1.5997947143422556E-5	0.001151852194326424	0.0	0.033019762904024155	4.7993841430267665E-5
44-45	1.5997947143422556E-5	0.0013118316657606496	0.0	0.04121871081502822	6.399178857369022E-5
46-47	1.5997947143422556E-5	0.00143981524290803	0.0	0.05041753042249618	6.399178857369022E-5
48-49	1.5997947143422556E-5	0.00143981524290803	0.0	0.05954435926781875	6.399178857369022E-5
50-51	1.5997947143422556E-5	0.0015997947143422556	0.0	0.06828723738169917	6.399178857369022E-5
52-53	1.5997947143422556E-5	0.001615792661485678	0.0	0.0772380888084441	7.998973571711278E-5
54-55	1.5997947143422556E-5	0.0016477885557725233	0.0	0.0855330244023087	9.598768286053533E-5
56-57	1.5997947143422556E-5	0.001695782397202791	0.0	0.09170023302609809	9.598768286053533E-5
58-59	1.5997947143422556E-5	0.001695782397202791	0.0	0.09742749810344337	9.598768286053533E-5
60-61	1.5997947143422556E-5	0.001695782397202791	0.0	0.10364270056866304	9.598768286053533E-5
62-63	1.5997947143422556E-5	0.0017437762386330587	0.0	0.10978591227173728	1.1198563000395789E-4
64-65	1.5997947143422556E-5	0.001959748525069263	0.0	0.11572914963551878	1.1198563000395789E-4
66-67	1.5997947143422556E-5	0.0019677474986409746	0.0	0.12178437262930421	1.1198563000395789E-4
68-69	1.5997947143422556E-5	0.002007742366499531	0.0	0.12750363873307777	1.1198563000395789E-4
70-71	1.5997947143422556E-5	0.002039738260786376	0.0	0.1323430177439631	1.1198563000395789E-4
72-73	1.5997947143422556E-5	0.002071734155073221	0.0	0.13643849221267929	1.7597741857764811E-4
74-75	1.5997947143422556E-5	0.0021277269700751998	0.0	0.13960608574707695	1.7597741857764811E-4
76-77	1.5997947143422556E-5	0.00219171875864889	0.0	0.14267769159861407	1.7597741857764811E-4
78-79	3.199589428684511E-5	0.0022557105472225804	0.0	0.14470943088582874	1.7597741857764811E-4
80-81	3.199589428684511E-5	0.0022557105472225804	0.0	0.1451973682737031	1.7597741857764811E-4
82-83	3.199589428684511E-5	0.0022557105472225804	0.0	0.1457492974501512	2.0797331286449323E-4
84-85	3.199589428684511E-5	0.0022637095207942914	0.0	0.14594927178944397	2.0797331286449323E-4
86-87	3.199589428684511E-5	0.002303704388652848	0.0	0.1460292615251611	2.0797331286449323E-4
88	3.199589428684511E-5	0.0024956797543739186	0.0	0.1460292615251611	2.0797331286449323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7730	0.0	52.99352	1
GTATCAA	14480	0.0	37.51378	1
TCAACGC	17350	0.0	30.839808	4
ATCAACG	17605	0.0	30.417831	3
CAACGCA	17795	0.0	30.092575	5
TATCAAC	18305	0.0	29.395193	2
AACGCAG	18455	0.0	29.016384	6
ACGCAGA	21175	0.0	25.228477	7
CGCAGAG	21780	0.0	24.429731	8
GTACATG	21140	0.0	23.959442	1
TACATGG	21485	0.0	23.177492	2
ACATGGG	21430	0.0	22.73864	3
GCAGAGT	25490	0.0	21.041445	9
TGGTATC	3695	0.0	20.093973	2
CATGGGG	14110	0.0	19.89814	4
GTGGTAT	3895	0.0	19.719505	1
GAGTACT	14210	0.0	19.352583	12-13
CAGAGTA	24995	0.0	18.778015	10-11
AGAGTAC	23175	0.0	18.301083	10-11
GTACTTT	16620	0.0	16.495064	14-15
>>END_MODULE