FastQCFastQC Report
Fri 27 May 2016
SRR2139169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6014203
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61250.050.0415571
GTATCAA114500.035.3812521
TCAACGC127800.030.7960134
ATCAACG131250.030.0192893
CAACGCA131450.030.0058385
AACGCAG136250.028.9487556
TATCAAC139250.028.4488852
ACGCAGA158850.024.8299337
CGCAGAG162600.024.2047828
GTACATG164600.022.7966751
TACATGG166800.022.0097392
ACATGGG165800.021.5753333
GCAGAGT201250.019.7047379
GAGTACT110600.018.98894312-13
CATGGGG117950.018.9277134
CAGAGTA193850.018.01267810-11
AGAGTAC174300.017.74319510-11
GTACTTT125750.016.68430514-15
GTGGTAT35400.015.7972141
TGGTATC32150.015.4024382
Produced by FastQC (version 0.11.5)