##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6014203
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.954779045536043	28.0	18.0	32.0	18.0	33.0
2	27.807240793169104	29.0	25.0	31.0	18.0	33.0
3	30.64565529297897	31.0	29.0	33.0	27.0	33.0
4	30.142051573583398	31.0	29.0	33.0	25.0	33.0
5	31.705632982458358	33.0	31.0	33.0	29.0	33.0
6	36.09489769467376	38.0	36.0	38.0	33.0	38.0
7	36.40685673563064	38.0	37.0	38.0	34.0	38.0
8	36.6562415668377	38.0	37.0	38.0	34.0	38.0
9	36.9512415859591	38.0	38.0	38.0	35.0	38.0
10-11	37.12714735435435	38.0	38.0	38.0	36.0	38.0
12-13	37.22680411685472	38.0	38.0	38.0	36.0	38.0
14-15	37.14622253355931	38.0	38.0	38.0	36.0	38.0
16-17	37.33802450632278	38.0	38.0	38.0	36.5	38.0
18-19	37.37042813486675	38.0	38.0	38.0	37.0	38.0
20-21	37.40644219994573	38.0	38.0	38.0	37.0	38.0
22-23	37.427525215326185	38.0	38.0	38.0	37.0	38.0
24-25	37.445758836517	38.0	38.0	38.0	37.0	38.0
26-27	37.40739751558543	38.0	38.0	38.0	37.0	38.0
28-29	37.38169748031682	38.0	38.0	38.0	37.0	38.0
30-31	37.39314878285113	38.0	38.0	38.0	37.0	38.0
32-33	37.383574912093366	38.0	38.0	38.0	37.0	38.0
34-35	37.35707573826222	38.0	38.0	38.0	37.0	38.0
36-37	37.33656549822118	38.0	38.0	38.0	37.0	38.0
38-39	37.350572657747065	38.0	38.0	38.0	37.0	38.0
40-41	37.350453610284674	38.0	38.0	38.0	37.0	38.0
42-43	37.315327637210345	38.0	38.0	38.0	37.0	38.0
44-45	37.23628907655173	38.0	38.0	38.0	37.0	38.0
46-47	37.27902094117999	38.0	38.0	38.0	37.0	38.0
48-49	37.242567562585236	38.0	38.0	38.0	37.0	38.0
50-51	37.20363123089297	38.0	38.0	38.0	37.0	38.0
52-53	37.18798991395344	38.0	38.0	38.0	37.0	38.0
54-55	37.168361182714676	38.0	38.0	38.0	36.0	38.0
56-57	37.132449824714094	38.0	38.0	38.0	36.0	38.0
58-59	37.12746782032421	38.0	38.0	38.0	36.0	38.0
60-61	37.136722341616604	38.0	38.0	38.0	36.0	38.0
62-63	37.14500470131245	38.0	38.0	38.0	36.0	38.0
64-65	37.106879669295196	38.0	38.0	38.0	36.0	38.0
66-67	37.13915701460239	38.0	38.0	38.0	36.0	38.0
68-69	37.13014756818947	38.0	38.0	38.0	36.0	38.0
70-71	37.13405292822172	38.0	38.0	38.0	36.0	38.0
72-73	37.121537832318815	38.0	38.0	38.0	36.0	38.0
74-75	37.119223486441555	38.0	38.0	38.0	36.0	38.0
76-77	37.10872058683222	38.0	38.0	38.0	36.0	38.0
78-79	37.093724530733326	38.0	38.0	38.0	36.0	38.0
80-81	37.06355221138052	38.0	38.0	38.0	36.0	38.0
82-83	37.03436008959385	38.0	38.0	38.0	36.0	38.0
84-85	37.019178469200455	38.0	38.0	38.0	36.0	38.0
86-87	37.01554885235984	38.0	38.0	38.0	36.0	38.0
88-89	37.00021918667467	38.0	38.0	38.0	36.0	38.0
90-91	36.97990542281924	38.0	38.0	38.0	36.0	38.0
92-93	36.95856370905568	38.0	38.0	38.0	35.5	38.0
94-95	36.935760239252055	38.0	38.0	38.0	35.0	38.0
96-97	36.94078845647316	38.0	38.0	38.0	35.0	38.0
98-99	36.944030504344425	38.0	38.0	38.0	35.0	38.0
100	35.62273009362942	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	17.0
21	86.0
22	360.0
23	1056.0
24	2528.0
25	5373.0
26	9278.0
27	15628.0
28	23889.0
29	34393.0
30	46908.0
31	61910.0
32	82501.0
33	115379.0
34	183160.0
35	363154.0
36	1149445.0
37	3919134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84727386155738	20.903235225016516	13.221785164218765	24.027705749207335
2	15.410929236331555	20.902351129156884	41.5633190662792	22.123400568232363
3	18.321206745927626	26.95311551701031	29.887008073417316	24.838669663644747
4	12.891968044970886	17.898683815408926	38.95785342760353	30.251494712016658
5	13.515024351522554	37.69615026296918	35.71208022077073	13.076745164737538
6	29.965910530689666	38.24668192174021	18.8250123790898	12.962395168480326
7	26.3641583099207	32.651092754933615	23.89669919688444	17.088049738261248
8	23.947163073810447	37.73956416170189	21.35985433148831	16.95341843299935
9	25.966882062344755	16.82038334921518	21.385975830879005	35.82675875756106
10-11	24.347153620713105	26.756966260860544	29.22541510910342	19.670465009322932
12-13	25.0742035145804	24.442332591700016	29.24005890722345	21.243404986496135
14-15	22.321004129724255	25.689688226353518	26.58782884448696	25.401478799435267
16-17	21.99677995571483	28.65575870984069	27.354264563401003	21.99319677104348
18-19	22.05857534240198	28.022308192789637	28.76751915424205	21.151597310566338
20-21	22.784969139497452	27.235815142929354	28.618152825997313	21.36106289157588
22-23	22.563879239913177	27.144173946111117	28.457263261442193	21.834683552533505
24-25	22.353957384355443	27.263923899185645	28.512272047840042	21.869846668618873
26-27	22.229218955072273	27.407533920803896	28.50924910246321	21.85399802166062
28-29	22.264548125858223	27.393526107904854	28.531859204199822	21.8100665620371
30-31	22.494369057690033	27.289337331380757	28.414393736529252	21.801899874399957
32-33	22.257387493763193	27.491051230169706	28.382071254415592	21.869490021651508
34-35	22.430431674154512	27.44437154475901	28.353503716866946	21.771693064219534
36-37	22.40109707223712	27.33070809073998	28.35140368607682	21.91679115094608
38-39	22.25151753337865	27.50620585833702	28.46163440008237	21.78064220820196
40-41	22.447223707697013	27.362383424391407	28.354012414658236	21.836380453253344
42-43	22.372628018231495	27.45673219454036	28.137627730298448	22.033012056929696
44-45	22.386397948318834	27.570870147669673	28.06816488183978	21.974567022171712
46-47	22.48254137034981	27.525961729125193	27.856162539084384	22.13533436144061
48-49	22.418147300290798	27.632309006293593	27.846372942290554	22.103170751125052
50-51	22.38803764025044	27.74937316985746	27.78288564108944	22.079703548802655
52-53	22.609616756459115	27.601763074676317	27.6925968890327	22.096023279831872
54-55	22.562762483167774	27.565770453888405	27.720079619708216	22.151387443235603
56-57	22.48664977079994	27.69916243797217	27.7191453439874	22.09504244724049
58-59	22.499780369094395	27.68415994653634	27.69596141691415	22.120098267455116
60-61	22.51960068943705	27.71752614799588	27.746579476750554	22.016293685816514
62-63	22.553370609041423	27.774054230206623	27.698329096001885	21.97424606475007
64-65	22.551694903966606	27.678660995269	27.69221325992694	22.077430840837454
66-67	22.57022088683139	27.855734930162836	27.534170879918314	22.039873303087465
68-69	22.545571129077096	27.778142125565342	27.61552384791252	22.06076289744504
70-71	22.567957803015233	27.531768760140054	27.678959741756753	22.221313695087964
72-73	22.689172274191254	27.626630344815567	27.526725892968646	22.157471488024534
74-75	22.42197885303381	27.74636733461559	27.60116331275438	22.23049049959622
76-77	22.605805742123607	27.517711659336264	27.612951972164133	22.263530626375992
78-79	22.503467437489675	27.524101593999433	27.573746785399088	22.398684183111804
80-81	22.554875956895025	27.61451194876618	27.501825200792247	22.32878689354655
82-83	22.606730026789954	27.498808469208264	27.62058561662088	22.2738758873809
84-85	22.630512367303844	27.507705021086963	27.54058279718231	22.32119981442688
86-87	22.483930180174326	27.553067444637424	27.608888787229606	22.354113587958643
88-89	22.585076152172135	27.53529576642078	27.538280498151725	22.34134758325536
90-91	22.621468964991582	27.582301476499875	27.533518183284222	22.262711375224317
92-93	22.639495976096743	27.536475513112602	27.53575491354795	22.288273597242707
94-95	22.50854844893769	27.537960728993138	27.573330994179795	22.38015982788938
96-97	22.647424849764793	27.633013839591523	27.61807023538223	22.101491075261457
98-99	23.739275548908996	29.537155583289405	27.8126722158267	18.9108966519749
100	30.23387118534308	40.688189741237416	29.077939073419497	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	840.0
1	777.5
2	1025.5
3	1481.0
4	2033.0
5	2971.5
6	3675.0
7	4326.0
8	5028.0
9	5339.5
10	5323.0
11	5335.0
12	5880.0
13	7283.5
14	10053.0
15	13463.5
16	16104.5
17	17699.5
18	18130.5
19	17482.0
20	16634.0
21	16528.0
22	17765.0
23	21058.5
24	26774.5
25	35001.0
26	45988.0
27	58606.0
28	71493.0
29	85500.5
30	99111.5
31	112229.5
32	126963.0
33	143076.5
34	157909.0
35	172201.0
36	188109.0
37	201300.0
38	212150.5
39	221256.5
40	229586.0
41	236900.0
42	240604.5
43	244490.0
44	249460.0
45	253312.0
46	254406.0
47	254164.5
48	252369.5
49	249234.0
50	244987.5
51	238518.5
52	229537.0
53	217671.0
54	203543.0
55	189073.0
56	173152.5
57	155231.0
58	137375.5
59	117353.5
60	97575.0
61	79505.0
62	62641.0
63	47762.5
64	35458.5
65	26374.5
66	19458.5
67	14514.0
68	10912.5
69	8116.5
70	5916.5
71	4150.5
72	2928.5
73	2018.0
74	1362.5
75	882.0
76	529.5
77	303.0
78	202.5
79	141.5
80	94.0
81	65.0
82	39.0
83	21.0
84	13.0
85	8.5
86	7.0
87	6.0
88	4.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018955130047988071
3	0.0024109595236476056
4	1.662730705963866E-5
5	0.0
6	4.821919047295211E-4
7	0.0
8	0.0
9	0.0
10-11	1.662730705963866E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.0784710966146528E-4
24-25	0.0010060249253068063
26-27	0.00256931389424255
28-29	0.0015885476856648377
30-31	0.0024887983265952656
32-33	0.003218893336131936
34-35	9.36198177773836E-4
36-37	8.388427828273783E-6
38-39	5.05963667255018E-5
40-41	1.6963388850829642E-5
42-43	6.057221462083585E-4
44-45	0.0
46-47	0.0
48-49	2.0950671643615106E-4
50-51	0.006228761287650354
52-53	0.0
54-55	0.0030444843808576753
56-57	0.04845239468962474
58-59	0.0603972033153089
60-61	0.038145996497528936
62-63	0.00871899170033269
64-65	0.0
66-67	0.0
68-69	7.023268372846336E-4
70-71	0.0028852625962800626
72-73	0.010792775325876476
74-75	0.0052173408386181706
76-77	0.004202907701779034
78-79	0.004136928134480668
80-81	0.016797894126511018
82-83	0.05560949586121744
84-85	0.036919820912115084
86-87	0.009959307185681719
88-89	0.00876425386617615
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	30.0
20-21	92.0
22-23	246.0
24-25	381.0
26-27	1090.0
28-29	2986.0
30-31	10544.0
32-33	13196.0
34-35	18271.0
36-37	29400.0
38-39	34714.0
40-41	33603.0
42-43	38046.0
44-45	45273.0
46-47	47086.0
48-49	45274.0
50-51	42357.0
52-53	40605.0
54-55	44297.0
56-57	47788.0
58-59	47704.0
60-61	46665.0
62-63	44795.0
64-65	47629.0
66-67	50912.0
68-69	52068.0
70-71	51421.0
72-73	48313.0
74-75	48730.0
76-77	51376.0
78-79	53835.0
80-81	53447.0
82-83	50523.0
84-85	49375.0
86-87	50867.0
88-89	53983.0
90-91	54910.0
92-93	53930.0
94-95	61408.0
96-97	185695.0
98-99	1396458.0
100-101	2964880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.8765374141487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87189853805089	78.94635893235703
2	6.4928564138866465	11.281537663314372
3	1.4904868790965888	3.8846501735139762
4	0.5338767557670719	1.855254557877694
5	0.24197209480721463	1.0510848873849459
6	0.1259214066053535	0.6563769475315335
7	0.07673211204330911	0.4666354141958037
8	0.04131903544175589	0.2871723782777794
9	0.032835930714947024	0.2567404766956941
>10	0.09011689222161626	1.1663007513990904
>50	0.0016845847226372496	0.09697072045079554
>100	2.993566419773967E-4	0.0509170970013362
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.662730705963866E-5
2	0.0	3.325461411927732E-5	0.0	0.0	1.662730705963866E-5
3	0.0	3.325461411927732E-5	0.0	0.0	1.662730705963866E-5
4	0.0	9.976384235783195E-5	0.0	0.0	3.325461411927732E-5
5	0.0	1.1639114941747062E-4	0.0	0.0	3.325461411927732E-5
6	0.0	1.1639114941747062E-4	0.0	0.0	3.325461411927732E-5
7	0.0	1.1639114941747062E-4	0.0	0.0	3.325461411927732E-5
8	0.0	1.3301845647710928E-4	0.0	0.0	4.9881921178915976E-5
9	0.0	2.4940960589457987E-4	0.0	0.0	6.650922823855464E-5
10-11	0.0	2.4940960589457987E-4	0.0	9.976384235783195E-5	6.650922823855464E-5
12-13	0.0	2.5772325942439924E-4	0.0	9.976384235783195E-5	6.650922823855464E-5
14-15	0.0	3.3254614119277316E-4	0.0	1.0807749588765128E-4	1.0807749588765128E-4
16-17	0.0	3.4917344825241185E-4	0.0	1.1639114941747062E-4	1.1639114941747062E-4
18-19	0.0	3.907417159015085E-4	0.0	1.1639114941747062E-4	1.1639114941747062E-4
20-21	0.0	4.572509441400631E-4	0.0	1.1639114941747062E-4	1.1639114941747062E-4
22-23	0.0	4.988192117891597E-4	0.0	1.3301845647710928E-4	1.2470480294728993E-4
24-25	0.0	5.487011329680757E-4	0.0	1.6627307059638658E-4	1.3301845647710928E-4
26-27	0.0	5.985830541469918E-4	0.0	1.9121403118584459E-4	1.3301845647710928E-4
28-29	0.0	6.06896707676811E-4	0.0	3.9074171590150844E-4	1.3301845647710928E-4
30-31	0.0	7.31601510624101E-4	0.0	0.0011057159194659708	1.4133211000692862E-4
32-33	0.0	7.31601510624101E-4	0.0	0.003001228924264778	1.4964576353674794E-4
34-35	0.0	8.313653529819329E-4	0.0	0.005378933833793106	1.6627307059638658E-4
36-37	0.0	9.311291953397649E-4	0.0	0.0089039229304365	1.6627307059638658E-4
38-39	0.0	9.643838094590422E-4	0.0	0.01447407079541545	1.8290037765602524E-4
40-41	0.0	0.0010475203447572355	0.0	0.019986023085685665	1.8290037765602524E-4
42-43	0.0	0.0010807749588765128	0.0	0.02526519307712094	1.9121403118584459E-4
44-45	0.0	0.001188852454764164	0.0	0.031417296689187246	1.995276847156639E-4
46-47	0.0	0.001263675336532538	0.0	0.037694105104200834	1.995276847156639E-4
48-49	0.0	0.0012803026435921768	0.0	0.04453624195924215	1.995276847156639E-4
50-51	0.0	0.0015463395565463952	0.0	0.051353437853694	1.995276847156639E-4
52-53	0.0	0.0015795941706656725	0.0	0.05802930163813892	2.0784133824548322E-4
54-55	0.0	0.0016294760918445885	0.0	0.06506265252436608	2.1615499177530257E-4
56-57	0.0	0.0016793580130235045	0.0	0.07202118052882486	2.1615499177530257E-4
58-59	0.0	0.0016793580130235045	0.0	0.07843932105384538	2.1615499177530257E-4
60-61	0.0	0.0016959853200831433	0.0	0.08463299293356077	2.3278229883494123E-4
62-63	0.0	0.0017209262806726013	0.0	0.09016988618442046	2.4940960589457987E-4
64-65	0.0	0.0019287676189180844	0.0	0.09633030345001657	2.4940960589457987E-4
66-67	0.0	0.0019453949259777231	0.0	0.10225793841677774	2.4940960589457987E-4
68-69	0.0	0.001970335886567181	0.0	0.10792785012411453	2.4940960589457987E-4
70-71	0.0	0.001995276847156639	0.0	0.113290156650848	2.4940960589457987E-4
72-73	0.0	0.0020285314612759164	0.0	0.11825340780815013	2.4940960589457987E-4
74-75	0.0	0.002053472421865374	0.0	0.121894788054211	2.4940960589457987E-4
76-77	0.0	0.002095040689514471	0.0	0.12513711293084054	2.4940960589457987E-4
78-79	0.0	0.002119981650103929	0.0	0.12721552631329539	2.4940960589457987E-4
80-81	0.0	0.0021282953036337485	0.0	0.12783905032803183	2.5772325942439924E-4
82-83	0.0	0.002136608957163568	0.0	0.12825473300452278	2.6603691295421856E-4
84-85	0.0	0.002161549917753026	0.0	0.12848751530335772	2.6603691295421856E-4
86-87	0.0	0.0021781772248126645	0.0	0.1285623381851261	2.6603691295421856E-4
88	0.0	0.00222805914599158	0.0	0.1285623381851261	2.6603691295421856E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6125	0.0	50.041557	1
GTATCAA	11450	0.0	35.381252	1
TCAACGC	12780	0.0	30.796013	4
ATCAACG	13125	0.0	30.019289	3
CAACGCA	13145	0.0	30.005838	5
AACGCAG	13625	0.0	28.948755	6
TATCAAC	13925	0.0	28.448885	2
ACGCAGA	15885	0.0	24.829933	7
CGCAGAG	16260	0.0	24.204782	8
GTACATG	16460	0.0	22.796675	1
TACATGG	16680	0.0	22.009739	2
ACATGGG	16580	0.0	21.575333	3
GCAGAGT	20125	0.0	19.704737	9
GAGTACT	11060	0.0	18.988943	12-13
CATGGGG	11795	0.0	18.927713	4
CAGAGTA	19385	0.0	18.012678	10-11
AGAGTAC	17430	0.0	17.743195	10-11
GTACTTT	12575	0.0	16.684305	14-15
GTGGTAT	3540	0.0	15.797214	1
TGGTATC	3215	0.0	15.402438	2
>>END_MODULE