FastQCFastQC Report
Fri 27 May 2016
SRR2139168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7468447
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84000.049.080661
GTATCAA153050.036.6901781
TCAACGC178250.030.7906324
ATCAACG181450.030.224723
CAACGCA184000.029.8284265
AACGCAG189400.029.1119356
TATCAAC190800.029.0763952
ACGCAGA226000.024.322517
GTACATG243100.023.9515741
CGCAGAG234100.023.3906258
TACATGG250250.022.8617132
ACATGGG248800.022.3318273
CATGGGG164850.020.0581674
GCAGAGT276850.019.91629
GTGGTAT43100.019.03311
GAGTACT147050.018.5755212-13
TGGTATC42500.017.9088922
CAGAGTA271000.017.6780410-11
AGAGTAC249350.017.2119510-11
GTACTTT170000.016.0432214-15
Produced by FastQC (version 0.11.5)