##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7468447
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.604989765609904	28.0	18.0	32.0	18.0	33.0
2	27.678687148747255	29.0	25.0	31.0	18.0	33.0
3	30.568286954436445	31.0	29.0	33.0	27.0	33.0
4	30.081935508145133	31.0	29.0	33.0	25.0	33.0
5	31.72065691836603	33.0	31.0	33.0	29.0	33.0
6	36.10144090197065	38.0	36.0	38.0	33.0	38.0
7	36.458718659983795	38.0	37.0	38.0	34.0	38.0
8	36.67250969311291	38.0	37.0	38.0	34.0	38.0
9	36.971064131538995	38.0	38.0	38.0	35.0	38.0
10-11	37.14114433696858	38.0	38.0	38.0	36.0	38.0
12-13	37.240892651444135	38.0	38.0	38.0	36.0	38.0
14-15	37.14799194531339	38.0	38.0	38.0	36.0	38.0
16-17	37.3415339895965	38.0	38.0	38.0	36.5	38.0
18-19	37.37272989953601	38.0	38.0	38.0	37.0	38.0
20-21	37.40774290360355	38.0	38.0	38.0	37.0	38.0
22-23	37.428727260033014	38.0	38.0	38.0	37.0	38.0
24-25	37.44612107320397	38.0	38.0	38.0	37.0	38.0
26-27	37.40855896455065	38.0	38.0	38.0	37.0	38.0
28-29	37.38477678212733	38.0	38.0	38.0	37.0	38.0
30-31	37.39372405191019	38.0	38.0	38.0	37.0	38.0
32-33	37.38247542879709	38.0	38.0	38.0	37.0	38.0
34-35	37.35701740115983	38.0	38.0	38.0	37.0	38.0
36-37	37.33538951181906	38.0	38.0	38.0	37.0	38.0
38-39	37.34757872861087	38.0	38.0	38.0	37.0	38.0
40-41	37.34639685000769	38.0	38.0	38.0	37.0	38.0
42-43	37.30892186052884	38.0	38.0	38.0	37.0	38.0
44-45	37.22699817805183	38.0	38.0	38.0	37.0	38.0
46-47	37.26358366913321	38.0	38.0	38.0	37.0	38.0
48-49	37.22279467016001	38.0	38.0	38.0	37.0	38.0
50-51	37.18160771609365	38.0	38.0	38.0	37.0	38.0
52-53	37.16309253819048	38.0	38.0	38.0	36.5	38.0
54-55	37.14519690747122	38.0	38.0	38.0	36.0	38.0
56-57	37.10929439000331	38.0	38.0	38.0	36.0	38.0
58-59	37.10088165353289	38.0	38.0	38.0	36.0	38.0
60-61	37.11251010176895	38.0	38.0	38.0	36.0	38.0
62-63	37.12318960442076	38.0	38.0	38.0	36.0	38.0
64-65	37.08707460666177	38.0	38.0	38.0	36.0	38.0
66-67	37.11897246381561	38.0	38.0	38.0	36.0	38.0
68-69	37.109495318888875	38.0	38.0	38.0	36.0	38.0
70-71	37.11667683851845	38.0	38.0	38.0	36.0	38.0
72-73	37.10302426535468	38.0	38.0	38.0	36.0	38.0
74-75	37.09968352462322	38.0	38.0	38.0	36.0	38.0
76-77	37.09079705577747	38.0	38.0	38.0	36.0	38.0
78-79	37.072621317539145	38.0	38.0	38.0	36.0	38.0
80-81	37.04320729106179	38.0	38.0	38.0	36.0	38.0
82-83	37.01385606509542	38.0	38.0	38.0	36.0	38.0
84-85	36.99561107088373	38.0	38.0	38.0	36.0	38.0
86-87	36.99525393990669	38.0	38.0	38.0	36.0	38.0
88-89	36.98134699734222	38.0	38.0	38.0	36.0	38.0
90-91	36.959664812465746	38.0	38.0	38.0	36.0	38.0
92-93	36.937979672916285	38.0	38.0	38.0	35.0	38.0
94-95	36.91571140634906	38.0	38.0	38.0	35.0	38.0
96-97	36.92138070543213	38.0	38.0	38.0	35.0	38.0
98-99	36.925911344739404	38.0	38.0	38.0	35.0	38.0
100	35.600184662115964	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	34.0
21	119.0
22	415.0
23	1348.0
24	3138.0
25	6667.0
26	11838.0
27	19575.0
28	30534.0
29	43952.0
30	60412.0
31	79776.0
32	104830.0
33	146307.0
34	229777.0
35	454009.0
36	1461087.0
37	4814622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65797789018252	21.21620465405994	13.207511548250928	23.918305907506607
2	15.373300902949197	20.961771111383374	41.757681294483824	21.90724669118361
3	18.40314065670297	27.291250543802313	29.77805902777452	24.527549771720192
4	12.818581962220527	17.9429806491229	39.03618784467507	30.2022495439815
5	13.460669935797897	37.77186877004014	35.62716586192551	13.14029543223645
6	29.621135566306933	38.24003999787371	19.010304353129595	13.128520082689764
7	26.123088240433383	32.6334377146949	24.1474566265249	17.09601741834681
8	23.834245593494874	37.77863055063523	21.478026154567342	16.90909770130256
9	25.951071220027405	16.764208141264174	21.571566351076736	35.71315428763169
10-11	24.345188497689012	26.906524207777068	29.159643229710277	19.58864406482365
12-13	25.026836235163753	24.52919596269479	29.294075461739233	21.14989234040223
14-15	22.262546684739142	25.66012719913524	26.724498413123904	25.352827703001708
16-17	21.855186225462937	28.616397759802005	27.531794762686275	21.996621252048786
18-19	21.918050700500384	27.971645242980237	29.00862120331041	21.101682853208974
20-21	22.718595865151563	27.223128596695982	28.76856011786729	21.289715420285162
22-23	22.52685515279698	27.08129651895465	28.599120049323407	21.79272827892496
24-25	22.217187329478158	27.248477982722015	28.73966993332115	21.794664754478678
26-27	22.08639135801171	27.370688803744336	28.714464200328628	21.828455637915326
28-29	22.095373196406577	27.372793208047092	28.76069363542414	21.77113996012219
30-31	22.33030476958903	27.286154024367143	28.631196177432084	21.752345028611742
32-33	22.128013612795115	27.428337843724925	28.573165582817943	21.87048296066202
34-35	22.281452428166407	27.399512042308977	28.60344994695482	21.715585582569798
36-37	22.29755298873278	27.30543387762455	28.532983420243564	21.86402971339911
38-39	22.121501034236058	27.48335711309761	28.64679443969877	21.748347412967558
40-41	22.295248836470087	27.310421829549213	28.591210519722697	21.803118814258006
42-43	22.203260910484023	27.407772113822293	28.363197456777193	22.025769518916487
44-45	22.210284937022134	27.513976281906704	28.29519447143883	21.980544309632332
46-47	22.317273680188187	27.5010254198963	28.087818011631942	22.09388288828357
48-49	22.31277055541971	27.546461384157002	28.044908803579027	22.09585925684426
50-51	22.278588679093364	27.653250498197153	28.00184931468715	22.066311508022334
52-53	22.406120675596334	27.57444760293882	27.90389444804051	22.11553727342433
54-55	22.41786360280044	27.55794549699836	27.86964417569698	22.15454672450422
56-57	22.36335520963026	27.641055806832064	27.86688226425535	22.128706719282327
58-59	22.328026981150785	27.61058024568948	27.925572454127455	22.13582031903228
60-61	22.38763212538072	27.646420047313736	27.921765684149868	22.044182143155677
62-63	22.394718022210316	27.676974728013437	27.937067778954	21.991239470822247
64-65	22.400223068492757	27.629140261922885	27.865309844190616	22.105326825393746
66-67	22.431137746638672	27.68479370641139	27.773844062897158	22.11022448405278
68-69	22.368568016761056	27.612643058746805	27.886461146849857	22.13232777764228
70-71	22.430380251849886	27.398023146201844	27.95962416264243	22.211972439305836
72-73	22.519978488064034	27.527291244270508	27.754922713114325	22.19780755455113
74-75	22.31913734048607	27.635582579500806	27.80006023441695	22.24521984559617
76-77	22.457369325012927	27.515477946788437	27.749358633833925	22.277794094364708
78-79	22.35694557494292	27.50035745979233	27.74865263893872	22.39404432632603
80-81	22.40769144674928	27.58765824830106	27.6868951751572	22.317755129792456
82-83	22.51297377181529	27.50579021667725	27.748559027292302	22.232676984215157
84-85	22.491916157421443	27.52673086426335	27.675711780970126	22.305641197345086
86-87	22.381757742479614	27.58195930679998	27.767776414640327	22.268506536080082
88-89	22.50167977538284	27.49009390506558	27.674580516307255	22.33364580324432
90-91	22.517087911387648	27.58574499250474	27.635590319054913	22.261576777052692
92-93	22.552381094788	27.567672496544184	27.631212741087342	22.248733667580474
94-95	22.427178585039716	27.514897440492263	27.72173272187154	22.336191252596482
96-97	22.588348180997965	27.602656452859627	27.72716908551472	22.08182628062769
98-99	23.6061732801872	29.55964066868939	27.92710254427192	18.90708350685149
100	30.176451797303205	40.616810697512804	29.206737505183987	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1066.0
1	1024.0
2	1557.0
3	2344.5
4	3065.5
5	4423.5
6	5642.0
7	6760.5
8	7828.5
9	8234.0
10	7893.0
11	7651.5
12	8519.5
13	10567.0
14	14349.5
15	18716.5
16	22094.0
17	24100.0
18	24260.5
19	23031.5
20	21764.0
21	21357.5
22	22866.0
23	27233.5
24	34891.0
25	45777.5
26	59687.5
27	75570.0
28	91839.5
29	109995.0
30	127284.0
31	143952.0
32	163957.5
33	184333.5
34	201690.0
35	219351.5
36	238939.0
37	254523.0
38	268754.5
39	280221.5
40	287561.0
41	295054.5
42	300162.0
43	304864.0
44	310606.0
45	315380.5
46	316726.5
47	315978.5
48	314696.0
49	311650.5
50	306445.0
51	298718.0
52	286856.0
53	272157.5
54	255525.5
55	236419.5
56	214366.5
57	190958.0
58	169006.5
59	145125.5
60	121899.0
61	100276.5
62	79727.0
63	61692.5
64	45818.5
65	33571.5
66	24596.0
67	18289.0
68	13541.0
69	9784.0
70	7070.0
71	4967.0
72	3358.0
73	2202.0
74	1433.0
75	896.0
76	580.5
77	390.5
78	255.5
79	171.0
80	111.5
81	70.0
82	44.0
83	26.5
84	18.5
85	14.0
86	10.0
87	8.5
88	6.5
89	4.0
90	3.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019013323653498512
3	0.0026779329089434527
4	0.0
5	0.0
6	6.694832272358632E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.9415463331955949E-4
24-25	0.0011850604451127205
26-27	0.002892578280232314
28-29	0.001801635389316329
30-31	0.002493686348153204
32-33	0.0034662252290009396
34-35	0.0011113398151161612
36-37	1.352477745993048E-5
38-39	5.442628571895082E-5
40-41	1.3698053129953773E-5
42-43	5.998391190114597E-4
44-45	0.0
46-47	0.0
48-49	1.6980436838718113E-4
50-51	0.006202542785589802
52-53	0.0
54-55	0.0029824999096541996
56-57	0.049800621746115256
58-59	0.06137727650537014
60-61	0.039836781946672326
62-63	0.008796391353938749
64-65	0.0
66-67	0.0
68-69	6.369086380967057E-4
70-71	0.0031570359139536514
72-73	0.011224705911117476
74-75	0.005309329815808762
76-77	0.004571858057425618
78-79	0.004509037176273676
80-81	0.017536165437876974
82-83	0.05744717671255111
84-85	0.03773822519850969
86-87	0.010331163560167014
88-89	0.008837628713160524
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	31.0
20-21	89.0
22-23	271.0
24-25	440.0
26-27	1445.0
28-29	3862.0
30-31	14855.0
32-33	18374.0
34-35	25617.0
36-37	41616.0
38-39	49772.0
40-41	47886.0
42-43	52864.0
44-45	63533.0
46-47	64965.0
48-49	62806.0
50-51	58889.0
52-53	56041.0
54-55	61591.0
56-57	65642.0
58-59	65545.0
60-61	63709.0
62-63	60883.0
64-65	64445.0
66-67	68351.0
68-69	70592.0
70-71	69253.0
72-73	64795.0
74-75	65273.0
76-77	68306.0
78-79	71339.0
80-81	70806.0
82-83	66452.0
84-85	64546.0
86-87	66727.0
88-89	70160.0
90-91	71503.0
92-93	70024.0
94-95	77939.0
96-97	227687.0
98-99	1690843.0
100-101	3568680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.00345651743353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.26264632003979	77.62899577934024
2	6.873418181855602	11.822754434587107
3	1.624438708727794	4.191220315539202
4	0.5722979984972102	1.9687842411507623
5	0.2649455270179909	1.1393115556190148
6	0.13407461677863078	0.6918528284527522
7	0.08520908245056784	0.5129792932199474
8	0.04706781766720217	0.323839600808937
9	0.030136672236603556	0.23326721822527924
>10	0.10396769631107317	1.3458891807004076
>50	0.0014624773140577301	0.08409392085347216
>100	3.1042547835238746E-4	0.04381335446779279
>500	2.4475625144356598E-5	0.01319827703500477
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3389664544717262E-5	0.0	0.0	1.3389664544717262E-5
3	0.0	1.3389664544717262E-5	0.0	0.0	1.3389664544717262E-5
4	0.0	5.355865817886905E-5	0.0	0.0	2.6779329089434525E-5
5	0.0	8.033798726830357E-5	0.0	0.0	2.6779329089434525E-5
6	1.3389664544717262E-5	8.033798726830357E-5	0.0	0.0	2.6779329089434525E-5
7	1.3389664544717262E-5	8.033798726830357E-5	0.0	0.0	2.6779329089434525E-5
8	1.3389664544717262E-5	8.033798726830357E-5	0.0	0.0	4.0168993634151784E-5
9	1.3389664544717262E-5	1.2050698090245536E-4	0.0	1.3389664544717262E-5	4.0168993634151784E-5
10-11	1.3389664544717262E-5	1.2050698090245536E-4	0.0	1.3389664544717262E-5	4.0168993634151784E-5
12-13	1.3389664544717262E-5	1.2050698090245536E-4	0.0	1.3389664544717262E-5	4.0168993634151784E-5
14-15	1.3389664544717262E-5	2.0084496817075894E-4	0.0	1.3389664544717262E-5	4.0168993634151784E-5
16-17	2.6779329089434525E-5	2.0084496817075894E-4	0.0	1.3389664544717262E-5	5.355865817886905E-5
18-19	2.6779329089434525E-5	2.4770879407726935E-4	0.0	1.3389664544717262E-5	5.355865817886905E-5
20-21	2.6779329089434525E-5	2.9457261998377975E-4	0.0	1.3389664544717262E-5	5.355865817886905E-5
22-23	2.6779329089434525E-5	3.4143644589029016E-4	0.0	1.3389664544717262E-5	7.364315499594494E-5
24-25	2.6779329089434525E-5	4.016899363415179E-4	0.0	2.6779329089434525E-5	9.372765181302083E-5
26-27	2.6779329089434525E-5	4.552485945203869E-4	0.0	2.6779329089434525E-5	9.372765181302083E-5
28-29	2.6779329089434525E-5	4.6194342679274555E-4	0.0	1.6067597453660714E-4	9.372765181302083E-5
30-31	2.6779329089434525E-5	5.221969172439732E-4	0.0	9.97530008581436E-4	1.0042248408537947E-4
32-33	2.6779329089434525E-5	5.221969172439732E-4	0.0	0.0030059796902890254	1.071173163577381E-4
34-35	2.6779329089434525E-5	6.092297367846354E-4	0.0	0.005744166089683705	1.2050698090245536E-4
36-37	2.6779329089434525E-5	6.962625563252976E-4	0.0	0.010169450221712761	1.2050698090245536E-4
38-39	3.3474161361793154E-5	6.962625563252976E-4	0.0	0.01681741866816488	1.2050698090245536E-4
40-41	4.0168993634151784E-5	7.766005435936011E-4	0.0	0.023994278864133335	1.2050698090245536E-4
42-43	4.0168993634151784E-5	8.16769537227753E-4	0.0	0.03155274449962623	1.2050698090245536E-4
44-45	4.0168993634151784E-5	9.506661826749257E-4	0.0	0.03933213960010696	1.2050698090245536E-4
46-47	4.0168993634151784E-5	0.0010577834990326638	0.0	0.04733915899784788	1.2050698090245536E-4
48-49	4.0168993634151784E-5	0.0010577834990326638	0.0	0.0553461783955888	1.2050698090245536E-4
50-51	4.0168993634151784E-5	0.0011649008153904019	0.0	0.06386869987830134	1.2050698090245536E-4
52-53	4.0168993634151784E-5	0.0011715956476627603	0.0	0.07247155934828217	1.2050698090245536E-4
54-55	4.0168993634151784E-5	0.0012050698090245535	0.0	0.08009027847422631	1.2050698090245536E-4
56-57	4.0168993634151784E-5	0.0012452388026587054	0.0	0.08677172108204022	1.3389664544717262E-4
58-59	4.686382590651042E-5	0.00127201813174814	0.0	0.09378790530347206	1.6737080680896577E-4
60-61	5.355865817886905E-5	0.001285407796292857	0.0	0.10065010838263966	1.740656390813244E-4
62-63	6.694832272358631E-5	0.0012987974608375745	0.0	0.10707045253183159	1.740656390813244E-4
64-65	6.694832272358631E-5	0.001486252764463616	0.0	0.11292843077014539	1.740656390813244E-4
66-67	8.033798726830357E-5	0.0014929475967359745	0.0	0.11886005216345513	1.8076047135368302E-4
68-69	8.033798726830357E-5	0.0015398114226424851	0.0	0.12483184255039903	1.8745530362604165E-4
70-71	8.033798726830357E-5	0.0015532010871872023	0.0	0.13052244998190388	1.8745530362604165E-4
72-73	9.372765181302083E-5	0.001559895919459561	0.0	0.13481383746848574	2.0084496817075894E-4
74-75	9.372765181302083E-5	0.0016000649130937128	0.0	0.13814786394012035	2.0084496817075894E-4
76-77	1.0042248408537947E-4	0.0016804029003620164	0.0	0.14141494208903135	2.0753980044311757E-4
78-79	1.071173163577381E-4	0.0017138770617238096	0.0	0.14373804888753983	2.142346327154762E-4
80-81	1.071173163577381E-4	0.0017138770617238096	0.0	0.14452803909567813	2.2092946498783483E-4
82-83	1.071173163577381E-4	0.001720571893996168	0.0	0.1450502360129221	2.2762429726019346E-4
84-85	1.071173163577381E-4	0.0017406563908132441	0.0	0.1453247241360888	2.2762429726019346E-4
86-87	1.071173163577381E-4	0.0017540460553579613	0.0	0.14545862078153599	2.2762429726019346E-4
88	1.071173163577381E-4	0.0017808253844473958	0.0	0.14546531561380832	2.2762429726019346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8400	0.0	49.08066	1
GTATCAA	15305	0.0	36.690178	1
TCAACGC	17825	0.0	30.790632	4
ATCAACG	18145	0.0	30.22472	3
CAACGCA	18400	0.0	29.828426	5
AACGCAG	18940	0.0	29.111935	6
TATCAAC	19080	0.0	29.076395	2
ACGCAGA	22600	0.0	24.32251	7
GTACATG	24310	0.0	23.951574	1
CGCAGAG	23410	0.0	23.390625	8
TACATGG	25025	0.0	22.861713	2
ACATGGG	24880	0.0	22.331827	3
CATGGGG	16485	0.0	20.058167	4
GCAGAGT	27685	0.0	19.9162	9
GTGGTAT	4310	0.0	19.0331	1
GAGTACT	14705	0.0	18.57552	12-13
TGGTATC	4250	0.0	17.908892	2
CAGAGTA	27100	0.0	17.67804	10-11
AGAGTAC	24935	0.0	17.21195	10-11
GTACTTT	17000	0.0	16.04322	14-15
>>END_MODULE