FastQCFastQC Report
Fri 27 May 2016
SRR2139167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9983263
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112300.053.8174061
GTATCAA211450.037.6545641
TCAACGC259800.030.074054
ATCAACG262550.029.7756633
CAACGCA264300.029.5620085
AACGCAG272600.028.6774856
TATCAAC275950.028.51492
ACGCAGA310300.025.1933067
CGCAGAG321200.024.2722998
GTACATG301350.023.7453671
TACATGG298550.023.3712
ACATGGG295250.022.7836443
GCAGAGT382550.020.4906629
CATGGGG203200.020.0295964
GAGTACT231750.019.24687812-13
AGAGTAC342950.018.79149410-11
CAGAGTA370500.018.60266310-11
GTACTTT266400.016.73560114-15
AGTACTT258000.016.40033712-13
ATGGGGG115200.015.9887515
Produced by FastQC (version 0.11.5)