##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9983263
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.108296656113335	18.0	18.0	30.0	18.0	33.0
2	30.50180997936246	31.0	29.0	33.0	27.0	33.0
3	31.660064650205047	33.0	31.0	33.0	29.0	33.0
4	32.57487516856963	33.0	33.0	33.0	31.0	34.0
5	32.99124214197302	33.0	33.0	34.0	32.0	34.0
6	36.97829176693031	38.0	38.0	38.0	35.0	38.0
7	37.063770432572994	38.0	38.0	38.0	36.0	38.0
8	37.058907793974775	38.0	38.0	38.0	36.0	38.0
9	37.279418262345686	38.0	38.0	38.0	36.0	38.0
10-11	37.31244173372974	38.0	38.0	38.0	37.0	38.0
12-13	37.36615698694905	38.0	38.0	38.0	37.0	38.0
14-15	37.27574726820279	38.0	38.0	38.0	36.5	38.0
16-17	37.34233661879888	38.0	38.0	38.0	37.0	38.0
18-19	37.388236441331856	38.0	38.0	38.0	37.0	38.0
20-21	37.40583046850803	38.0	38.0	38.0	37.0	38.0
22-23	37.426797714614324	38.0	38.0	38.0	37.0	38.0
24-25	37.437837876182066	38.0	38.0	38.0	37.0	38.0
26-27	37.40658952053202	38.0	38.0	38.0	37.0	38.0
28-29	37.385228367218986	38.0	38.0	38.0	37.0	38.0
30-31	37.38335452464368	38.0	38.0	38.0	37.0	38.0
32-33	37.36532064679966	38.0	38.0	38.0	37.0	38.0
34-35	37.34096653661179	38.0	38.0	38.0	37.0	38.0
36-37	37.318576509253916	38.0	38.0	38.0	37.0	38.0
38-39	37.312868156388745	38.0	38.0	38.0	37.0	38.0
40-41	37.2997822277934	38.0	38.0	38.0	37.0	38.0
42-43	37.262003214718945	38.0	38.0	38.0	37.0	38.0
44-45	37.168202117630315	38.0	38.0	38.0	37.0	38.0
46-47	37.18958143531442	38.0	38.0	38.0	37.0	38.0
48-49	37.146247286074264	38.0	38.0	38.0	36.5	38.0
50-51	37.08827597987769	38.0	38.0	38.0	36.0	38.0
52-53	37.0595601647559	38.0	38.0	38.0	36.0	38.0
54-55	37.025865931803196	38.0	38.0	38.0	36.0	38.0
56-57	36.977945580031054	38.0	38.0	38.0	36.0	38.0
58-59	36.963130026538536	38.0	38.0	38.0	36.0	38.0
60-61	36.96818523033166	38.0	38.0	38.0	36.0	38.0
62-63	36.97155253449958	38.0	38.0	38.0	36.0	38.0
64-65	36.9272231717569	38.0	38.0	38.0	36.0	38.0
66-67	36.955815314843726	38.0	38.0	38.0	36.0	38.0
68-69	36.94234642440169	38.0	38.0	38.0	36.0	38.0
70-71	36.9454222090211	38.0	38.0	38.0	36.0	38.0
72-73	36.927807326174026	38.0	38.0	38.0	35.5	38.0
74-75	36.91655425390296	38.0	38.0	38.0	35.0	38.0
76-77	36.8996621405399	38.0	38.0	38.0	35.0	38.0
78-79	36.87523516531195	38.0	38.0	38.0	35.0	38.0
80-81	36.840945152340865	38.0	38.0	38.0	35.0	38.0
82-83	36.799962484640105	38.0	38.0	38.0	35.0	38.0
84-85	36.77473854922762	38.0	38.0	38.0	35.0	38.0
86-87	36.766664643704544	38.0	38.0	38.0	35.0	38.0
88-89	36.74731500767012	38.0	38.0	38.0	35.0	38.0
90-91	36.723145520446835	38.0	38.0	38.0	34.5	38.0
92-93	36.692192114637194	38.0	38.0	38.0	34.0	38.0
94-95	36.657445373747606	38.0	38.0	38.0	34.0	38.0
96-97	36.65001275039756	38.0	38.0	38.0	34.0	38.0
98-99	36.65639602668493	38.0	38.0	38.0	34.0	38.0
100	35.1439571169754	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	11.0
20	24.0
21	148.0
22	571.0
23	1780.0
24	4222.0
25	9143.0
26	16785.0
27	28006.0
28	44273.0
29	64362.0
30	86963.0
31	112068.0
32	146153.0
33	196990.0
34	295988.0
35	538555.0
36	1692022.0
37	6745195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.111091333564985	18.598688625151915	15.31472225063088	23.975497790652213
2	14.41289508817312	21.120979801039518	42.19857623212377	22.267548878663597
3	18.09645014542098	26.33935301562593	31.171586738968966	24.392610099984125
4	12.351864550885272	17.662974286360512	39.55974510135064	30.425416061403578
5	13.147765414975044	37.30832294010485	36.10613083117214	13.43778081374797
6	29.334364262923906	38.0895027587784	19.348080274276153	13.228052704021536
7	25.773356867388948	32.539631581377755	24.47524421624473	17.211767334988572
8	23.733412612689857	37.05117254749274	22.044285520675956	17.17112931914145
9	25.757770780956086	16.377350771987075	21.85917570237306	36.005702744683774
10-11	24.05256798566601	26.453571665575254	29.740893821032955	19.752966527725782
12-13	24.774379879604496	24.266980645506383	29.766184663270916	21.192454811618205
14-15	22.071385878544923	25.400628031135714	27.306773346550123	25.221212743769243
16-17	21.577714620961103	28.369066306276814	28.029162409124154	22.024056663637932
18-19	21.60344769039942	27.595626800576124	29.728140989574246	21.072784519450206
20-21	22.38791064504794	26.889029324877523	29.547616815296422	21.175443214778113
22-23	22.16219865688744	26.76227552809891	29.380404412291337	21.695121402722307
24-25	21.864528350652304	26.864738722800634	29.52922720292197	21.741505723625092
26-27	21.770593286591126	26.99172107684411	29.514181211363965	21.723504425200797
28-29	21.78311757728136	26.97806418198661	29.561419433583303	21.67739880714873
30-31	21.999286047003345	26.912702947006995	29.42329890016333	21.664712105826332
32-33	21.781537361389724	27.111781187651378	29.346252304518632	21.760429146440263
34-35	21.93604435441609	27.0584874453909	29.375649301870325	21.629818898322682
36-37	21.973898449108457	26.923946662698654	29.34596311384887	21.75619177434402
38-39	21.779569457592657	27.128872501777295	29.448334260765087	21.64322377986496
40-41	22.008438929836863	26.962544883017824	29.313621066077626	21.715395121067687
42-43	21.869044977462288	27.062419859412806	29.119161259252724	21.94937390387218
44-45	21.9468580661936	27.22758046336875	28.961212668080833	21.864348802356822
46-47	22.029109331786547	27.130613135386746	28.776088595208886	22.06418893761782
48-49	21.981113187083256	27.227054735340968	28.764734367819038	22.02709770975674
50-51	21.954244828167557	27.365154713290764	28.661657050313632	22.01894340822805
52-53	22.095346830270756	27.270425587163054	28.533915597292612	22.100311985273574
54-55	22.10619109264038	27.1537610678562	28.596586850163845	22.14346098933958
56-57	22.02356840060295	27.25880073790698	28.614753786126695	22.102877075363374
58-59	21.975032262482316	27.233807270539355	28.68722653904849	22.103933927929834
60-61	21.988135533665442	27.22181080810563	28.758811088207146	22.031242570021778
62-63	21.975594832070474	27.22517987196603	28.818340358660187	21.980884937303305
64-65	21.891669927526234	27.14182597186173	28.85227733295947	22.114226767652568
66-67	21.88586269707672	27.12920335940946	28.831979985429417	22.1529539580844
68-69	21.83260389743561	27.23130542835494	28.785193762226776	22.15089691198267
70-71	21.907020702033492	26.956061095976576	28.704487391689927	22.432430810300012
72-73	22.065342532744918	27.035735118223435	28.66546862203263	22.233453726999016
74-75	21.97111888551996	27.26990175462455	28.578524225291247	22.180455134564244
76-77	22.12145421833886	27.22175991740154	28.29687746803355	22.359908396226054
78-79	22.121375064275448	27.25481128704551	28.221548107815508	22.40226554086353
80-81	22.154547013949784	27.322124852188708	28.154190380085133	22.36913775377638
82-83	22.238664669349482	27.285130762717696	28.209476818022505	22.266727749910316
84-85	22.294757517023424	27.36005064876363	28.077139087460935	22.268052746752016
86-87	22.176488122287584	27.393820333476636	28.17991957426645	22.249771969969334
88-89	22.276387669710115	27.41178203445748	28.06027809518095	22.25155220065145
90-91	22.31901149997062	27.45454796755072	28.021735042076074	22.204705490402585
92-93	22.43247507816607	27.4726223764224	27.980302701713793	22.114599843697736
94-95	22.26027655351075	27.464791575547554	28.06508091377048	22.209850957171216
96-97	22.39396130214784	27.52356712406405	28.130350169492544	21.952121404295568
98-99	23.364640083732137	29.488658884857845	28.363296690129097	18.783404341280924
100	29.741989264118157	40.49801769370133	29.759993042180522	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2190.0
1	2059.5
2	2839.0
3	4043.5
4	5098.0
5	7418.0
6	9638.0
7	12004.5
8	14168.0
9	14600.0
10	13659.0
11	12719.0
12	13726.5
13	17003.5
14	23609.5
15	31474.0
16	37058.5
17	39840.5
18	39674.5
19	37062.0
20	34026.5
21	32271.0
22	33641.5
23	39632.0
24	49926.0
25	64384.5
26	83798.5
27	105845.0
28	126787.5
29	149518.5
30	171923.0
31	193093.0
32	218999.0
33	246939.0
34	272419.0
35	297544.5
36	323948.5
37	344623.5
38	359218.0
39	371202.5
40	382993.0
41	391746.0
42	395577.0
43	401152.5
44	408837.5
45	414286.5
46	414914.5
47	414199.5
48	412175.0
49	407763.0
50	401076.5
51	390793.5
52	374968.0
53	354749.5
54	331332.5
55	306300.5
56	278378.0
57	248891.0
58	221051.5
59	188525.0
60	155696.0
61	125548.0
62	98127.0
63	74469.0
64	54383.5
65	40033.0
66	29234.5
67	21708.0
68	16392.5
69	11960.0
70	8460.0
71	5895.0
72	4090.0
73	2709.5
74	1730.0
75	1101.5
76	696.0
77	423.5
78	268.0
79	169.5
80	109.0
81	71.5
82	48.5
83	30.5
84	24.0
85	21.0
86	14.5
87	8.0
88	4.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017529338854440676
3	0.0017829841806231087
4	1.0016765059680386E-5
5	0.0
6	4.0067060238721546E-4
7	0.0
8	0.0
9	0.0
10-11	5.008382529840193E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.0017088150676239E-4
24-25	9.116043324647045E-4
26-27	0.0024295258422151705
28-29	0.0015783906988993757
30-31	0.0021163373722612364
32-33	0.003130208587955872
34-35	7.705816309858832E-4
36-37	0.0
38-39	3.048532949407868E-5
40-41	0.0
42-43	5.245187578963856E-4
44-45	0.0
46-47	0.0
48-49	2.0559356840058483E-4
50-51	0.006028742428253048
52-53	0.0
54-55	0.002422727406151418
56-57	0.043270722691781636
58-59	0.05444356943237255
60-61	0.035230274079787065
62-63	0.008063125437404985
64-65	0.0
66-67	0.0
68-69	5.820010024246969E-4
70-71	0.002668730329403482
72-73	0.009868164271059325
74-75	0.0046618875274086835
76-77	0.0038343343634670885
78-79	0.0037575705607196604
80-81	0.015467772504514723
82-83	0.05223433604829995
84-85	0.033854149405816436
86-87	0.009381547205531499
88-89	0.008236860558743621
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	42.0
20-21	166.0
22-23	495.0
24-25	821.0
26-27	2107.0
28-29	5428.0
30-31	17761.0
32-33	22179.0
34-35	30616.0
36-37	49101.0
38-39	56818.0
40-41	58632.0
42-43	67689.0
44-45	81699.0
46-47	84473.0
48-49	81215.0
50-51	72292.0
52-53	67378.0
54-55	74093.0
56-57	78539.0
58-59	85132.0
60-61	84363.0
62-63	82282.0
64-65	86573.0
66-67	92714.0
68-69	95435.0
70-71	95567.0
72-73	89882.0
74-75	89833.0
76-77	95021.0
78-79	99658.0
80-81	98643.0
82-83	93190.0
84-85	90923.0
86-87	94332.0
88-89	99694.0
90-91	101416.0
92-93	100061.0
94-95	112577.0
96-97	315001.0
98-99	2269310.0
100-101	4760112.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.28506602976397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.88712257528046	78.42201372663325
2	6.313742394264829	10.895633587681216
3	1.5313727123294651	3.9640378679857995
4	0.5795204314687562	2.0001583477951574
5	0.2713854085097216	1.1708253946387897
6	0.14266348217716046	0.7385836787815407
7	0.07630894264639428	0.4609025508433921
8	0.050556266552922544	0.34898006381898156
9	0.03124538703652012	0.24264092552145028
>10	0.11318368352941814	1.4995908761719141
>50	0.0022503320562512726	0.13539255410250384
>100	6.11065122138979E-4	0.08483748354111785
>500	1.8659516048768352E-5	0.014715743429571958
>1k	1.8659509964745693E-5	0.0216871990552984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0016765059680388E-5	1.0016765059680388E-5	0.0	0.0	0.0
3	1.0016765059680388E-5	1.0016765059680388E-5	0.0	0.0	0.0
4	1.0016765059680388E-5	1.0016765059680387E-4	0.0	1.0016765059680388E-5	0.0
5	2.0033530119360775E-5	1.0016765059680387E-4	0.0	2.0033530119360775E-5	0.0
6	5.008382529840193E-5	1.0016765059680387E-4	0.0	2.0033530119360775E-5	0.0
7	5.008382529840193E-5	1.0016765059680387E-4	0.0	2.0033530119360775E-5	0.0
8	5.008382529840193E-5	1.2020118071616464E-4	0.0	2.0033530119360775E-5	0.0
9	5.008382529840193E-5	1.9031853613392735E-4	0.0	2.0033530119360775E-5	0.0
10-11	5.008382529840193E-5	1.9031853613392735E-4	0.0	3.005029517904116E-5	1.0016765059680388E-5
12-13	5.008382529840193E-5	1.9031853613392735E-4	0.0	4.006706023872155E-5	1.0016765059680388E-5
14-15	5.509220782824213E-5	2.5041912649200967E-4	0.0	5.008382529840193E-5	1.0016765059680388E-5
16-17	8.514250300728328E-5	2.7045265661137044E-4	0.0	5.008382529840193E-5	1.0016765059680388E-5
18-19	1.0016765059680387E-4	3.1552809937993216E-4	0.0	6.510897288792251E-5	1.0016765059680388E-5
20-21	1.0016765059680387E-4	3.956622198573753E-4	0.0	7.01173554177627E-5	1.502514758952058E-5
22-23	1.0016765059680387E-4	4.808047228646586E-4	0.0	8.01341204774431E-5	2.0033530119360775E-5
24-25	1.3522632830568522E-4	5.258801656332203E-4	0.0	8.514250300728329E-5	2.0033530119360775E-5
26-27	1.502514758952058E-4	5.509220782824213E-4	0.0	1.1519279818632445E-4	2.0033530119360775E-5
28-29	1.502514758952058E-4	5.509220782824213E-4	0.0	4.1068736744689585E-4	2.0033530119360775E-5
30-31	1.502514758952058E-4	6.761316415284261E-4	0.0	0.0014724644637730169	2.0033530119360775E-5
32-33	1.5525985842504598E-4	6.811400240582663E-4	0.0	0.0039215635208648715	4.507544276856174E-5
34-35	1.652766234847264E-4	7.662825270655497E-4	0.0	0.007091869662253714	7.01173554177627E-5
36-37	1.7028500601456658E-4	8.51425030072833E-4	0.0	0.01263114074025697	7.01173554177627E-5
38-39	1.7028500601456658E-4	8.51425030072833E-4	0.0	0.02909870249837152	8.01341204774431E-5
40-41	1.7028500601456658E-4	9.465842981397966E-4	0.0	0.04751452506059392	9.015088553712348E-5
42-43	1.8030177107424697E-4	9.81642975848678E-4	0.0	0.0581172708762656	9.015088553712348E-5
44-45	1.8531015360408716E-4	0.001086819008975322	0.0	0.07107395648096218	9.015088553712348E-5
46-47	2.0033530119360773E-4	0.0011569363643930846	0.0	0.08488707549826144	9.015088553712348E-5
48-49	2.0534368372344792E-4	0.001166953129452765	0.0	0.09903074776253015	1.0016765059680387E-4
50-51	2.1536044878312833E-4	0.0012721291625794092	0.0	0.11387058519844664	1.0016765059680387E-4
52-53	2.2036883131296852E-4	0.0012721291625794092	0.0	0.16148527790963735	1.0016765059680387E-4
54-55	2.2036883131296852E-4	0.0012721291625794092	0.0	0.22038385646055803	1.0016765059680387E-4
56-57	2.303855963726489E-4	0.0013121962228181308	0.0	0.306758421570182	1.0016765059680387E-4
58-59	2.404023614323293E-4	0.001317204605347971	0.0	0.35842489574801345	1.0016765059680387E-4
60-61	2.404023614323293E-4	0.0013322297529374915	0.0	0.3770360452288996	1.0016765059680387E-4
62-63	2.404023614323293E-4	0.0013522632830568523	0.0	0.3918608575172266	1.0016765059680387E-4
64-65	2.404023614323293E-4	0.0015125315240117384	0.0	0.40001951265833624	1.0016765059680387E-4
66-67	2.454107439621695E-4	0.0015475902017206198	0.0	0.4076923546940514	1.0016765059680387E-4
68-69	2.6043589155169005E-4	0.001582648879429501	0.0	0.41526502907916985	1.0016765059680387E-4
70-71	2.6544427408153025E-4	0.0016427494697875834	0.0	0.42136523900051515	1.0016765059680387E-4
72-73	2.7045265661137044E-4	0.0016627829999069442	0.0	0.42665409095202644	1.3021794577584503E-4
74-75	2.804694216710508E-4	0.001707858442675506	0.0	0.43041037784940656	1.3021794577584503E-4
76-77	2.804694216710508E-4	0.00180802609327231	0.0	0.43382609473475753	1.3021794577584503E-4
78-79	2.804694216710508E-4	0.0018430847709811913	0.0	0.436320269234618	1.3021794577584503E-4
80-81	2.804694216710508E-4	0.0018430847709811913	0.0	0.4370214427887956	1.3522632830568522E-4
82-83	2.804694216710508E-4	0.0018480931535110314	0.0	0.43766251575261517	1.402347108355254E-4
84-85	2.804694216710508E-4	0.0018581099185707118	0.0	0.4380331360598233	1.402347108355254E-4
86-87	2.804694216710508E-4	0.0019081937438691137	0.0	0.4381883959182484	1.452430933653656E-4
88	2.904861867307312E-4	0.0020534368372344793	0.0	0.4382034210658379	1.502514758952058E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11230	0.0	53.817406	1
GTATCAA	21145	0.0	37.654564	1
TCAACGC	25980	0.0	30.07405	4
ATCAACG	26255	0.0	29.775663	3
CAACGCA	26430	0.0	29.562008	5
AACGCAG	27260	0.0	28.677485	6
TATCAAC	27595	0.0	28.5149	2
ACGCAGA	31030	0.0	25.193306	7
CGCAGAG	32120	0.0	24.272299	8
GTACATG	30135	0.0	23.745367	1
TACATGG	29855	0.0	23.371	2
ACATGGG	29525	0.0	22.783644	3
GCAGAGT	38255	0.0	20.490662	9
CATGGGG	20320	0.0	20.029596	4
GAGTACT	23175	0.0	19.246878	12-13
AGAGTAC	34295	0.0	18.791494	10-11
CAGAGTA	37050	0.0	18.602663	10-11
GTACTTT	26640	0.0	16.735601	14-15
AGTACTT	25800	0.0	16.400337	12-13
ATGGGGG	11520	0.0	15.988751	5
>>END_MODULE