FastQCFastQC Report
Fri 27 May 2016
SRR2139166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7009467
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.054.3615231
GTATCAA181950.039.3309481
TCAACGC230100.030.6452564
ATCAACG233700.030.1914923
CAACGCA234750.030.0204325
TATCAAC239300.029.8526272
AACGCAG241500.029.1986486
ACGCAGA278950.025.3385317
CGCAGAG286200.024.6236788
GTACATG276150.023.3275151
TACATGG275600.023.07092
ACATGGG272500.022.6120113
GTGGTAT40650.022.4039751
GCAGAGT327600.021.5501489
CATGGGG191200.020.5374934
TGGTATC41000.020.0729642
GAGTACT182850.019.29354912-13
CAGAGTA319350.019.16364710-11
AGAGTAC301150.018.87916210-11
GTACTTT200500.017.4910914-15
Produced by FastQC (version 0.11.5)