##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7009467
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.631626627245694	18.0	18.0	30.0	18.0	33.0
2	30.256415216734737	31.0	29.0	33.0	27.0	33.0
3	31.507895678801255	33.0	31.0	33.0	29.0	33.0
4	32.50099444080413	33.0	33.0	33.0	31.0	34.0
5	32.92989053233291	33.0	33.0	34.0	32.0	34.0
6	36.90320690574619	38.0	37.0	38.0	35.0	38.0
7	36.994989062649125	38.0	38.0	38.0	35.0	38.0
8	36.97596778756502	38.0	38.0	38.0	35.0	38.0
9	37.22033857923862	38.0	38.0	38.0	36.0	38.0
10-11	37.258587564503834	38.0	38.0	38.0	36.0	38.0
12-13	37.31354316954484	38.0	38.0	38.0	37.0	38.0
14-15	37.222964813159116	38.0	38.0	38.0	36.0	38.0
16-17	37.29868397982329	38.0	38.0	38.0	36.5	38.0
18-19	37.350874610009576	38.0	38.0	38.0	37.0	38.0
20-21	37.37179035758493	38.0	38.0	38.0	37.0	38.0
22-23	37.39702544446331	38.0	38.0	38.0	37.0	38.0
24-25	37.40726433787658	38.0	38.0	38.0	37.0	38.0
26-27	37.3774375325485	38.0	38.0	38.0	37.0	38.0
28-29	37.355967266976805	38.0	38.0	38.0	37.0	38.0
30-31	37.353290780492806	38.0	38.0	38.0	37.0	38.0
32-33	37.33391649645846	38.0	38.0	38.0	37.0	38.0
34-35	37.31239645911599	38.0	38.0	38.0	37.0	38.0
36-37	37.28865805802343	38.0	38.0	38.0	37.0	38.0
38-39	37.283239013672286	38.0	38.0	38.0	37.0	38.0
40-41	37.265128008218326	38.0	38.0	38.0	37.0	38.0
42-43	37.225423184199414	38.0	38.0	38.0	37.0	38.0
44-45	37.133277750435255	38.0	38.0	38.0	36.0	38.0
46-47	37.14056085790414	38.0	38.0	38.0	36.0	38.0
48-49	37.08849463078014	38.0	38.0	38.0	36.0	38.0
50-51	37.026619938167904	38.0	38.0	38.0	36.0	38.0
52-53	36.99437547028185	38.0	38.0	38.0	36.0	38.0
54-55	36.956725091028154	38.0	38.0	38.0	36.0	38.0
56-57	36.90346845199734	38.0	38.0	38.0	36.0	38.0
58-59	36.88483848237896	38.0	38.0	38.0	36.0	38.0
60-61	36.89225063802008	38.0	38.0	38.0	36.0	38.0
62-63	36.89549845530814	38.0	38.0	38.0	35.0	38.0
64-65	36.85235017416292	38.0	38.0	38.0	35.0	38.0
66-67	36.87949312446248	38.0	38.0	38.0	35.0	38.0
68-69	36.86185941675998	38.0	38.0	38.0	35.0	38.0
70-71	36.86759389863229	38.0	38.0	38.0	35.0	38.0
72-73	36.85051777787304	38.0	38.0	38.0	35.0	38.0
74-75	36.83906307109332	38.0	38.0	38.0	35.0	38.0
76-77	36.821128221824225	38.0	38.0	38.0	35.0	38.0
78-79	36.7964743808006	38.0	38.0	38.0	35.0	38.0
80-81	36.75873309620245	38.0	38.0	38.0	35.0	38.0
82-83	36.71714339933503	38.0	38.0	38.0	34.5	38.0
84-85	36.69099224873542	38.0	38.0	38.0	34.0	38.0
86-87	36.681603173883346	38.0	38.0	38.0	34.0	38.0
88-89	36.659994209710035	38.0	38.0	38.0	34.0	38.0
90-91	36.63605865390298	38.0	38.0	38.0	34.0	38.0
92-93	36.603605268578164	38.0	38.0	38.0	34.0	38.0
94-95	36.56988725511742	38.0	38.0	38.0	34.0	38.0
96-97	36.56494229180534	38.0	38.0	38.0	34.0	38.0
98-99	36.57397942471347	38.0	38.0	38.0	34.0	38.0
100	35.04662995581544	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	4.0
19	12.0
20	29.0
21	103.0
22	447.0
23	1330.0
24	3301.0
25	6946.0
26	12682.0
27	21085.0
28	33535.0
29	48447.0
30	64700.0
31	83166.0
32	108350.0
33	148338.0
34	223795.0
35	408477.0
36	1310786.0
37	4533929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86093364873535	18.0778224649606	15.600187574889787	23.461056311414264
2	14.875542330644679	21.26274247856179	41.5226360404438	22.33907915034973
3	18.343483132634283	26.52825503821048	30.883376366427967	24.24488546272727
4	12.650862041293582	17.54116254488394	38.913600706016595	30.894374707805888
5	13.393472000082173	37.49892823519963	35.331117187654925	13.776482577063279
6	29.660657627428154	37.64857106987149	19.09833025177475	13.592441050925608
7	26.470614670131127	32.57995222746608	23.704184640572528	17.245248461830265
8	24.547158863862258	36.49588477982705	21.527628277585155	17.42932807872553
9	26.56383145822642	16.225484762250826	21.148854827335658	36.06182895218709
10-11	24.6215297111749	26.32141645006675	28.97678953335539	20.080264305402963
12-13	25.504086116676206	24.1507521185277	29.029539621200872	21.315622143595224
14-15	22.781981853969782	25.104283963388376	26.78570282162681	25.32803136101504
16-17	22.026539250416615	28.05777529161632	27.446109668538277	22.469575789428784
18-19	22.090374346580134	27.175661145134143	29.304139672816774	21.429824835468946
20-21	23.16281348441756	26.37051539298165	29.009229704284095	21.4574414183167
22-23	22.87874281857281	26.238877111805238	28.889509190108143	21.992870879513813
24-25	22.45612255322452	26.447790232930572	29.027202533112117	22.06888468073279
26-27	22.28162082529944	26.553261419980593	29.083317023551803	22.081800731168162
28-29	22.320551291877088	26.506605804765236	29.112973334158493	22.059869569199183
30-31	22.604841836938157	26.452478899704396	28.87300429988784	22.069674963469605
32-33	22.31166318662017	26.699595455789844	28.856362122774147	22.132379234815836
34-35	22.492644832752422	26.63570351854871	28.868884099009623	22.00276754968925
36-37	22.480663202455734	26.49074286765695	28.839342987572504	22.189250942314814
38-39	22.276899070134053	26.7660472407966	28.956409443505628	22.000644245563723
40-41	22.56509781433602	26.548952304858968	28.834378979241887	22.051570901563128
42-43	22.42251950535959	26.666383578942703	28.549022793485047	22.362074122212665
44-45	22.466903650846223	26.78949240290212	28.446404141698018	22.29719980455364
46-47	22.603538286689606	26.73074839749216	28.183955530115455	22.48175778570278
48-49	22.52128356054631	26.85771907603694	28.12440749975672	22.49658986366003
50-51	22.46494978308227	27.01574856637664	27.981167665368563	22.538133985172525
52-53	22.713697688260236	26.862057553888697	27.84723819569887	22.577006562152192
54-55	22.715608819425327	26.77878867036186	27.868296853343903	22.637305656868914
56-57	22.60559398055544	26.957853238416895	27.89015695740146	22.546395823626206
58-59	22.603387861861915	26.906307893189197	27.91761686249415	22.57268738245474
60-61	22.6338118878239	26.959854312722985	27.9620724417526	22.444261357700515
62-63	22.595753572699582	26.9481130073364	28.007320075355636	22.448813344608382
64-65	22.598888180324877	26.860148898500913	28.02359213035987	22.51737079081434
66-67	22.669907818623123	26.96570440520715	27.814889108508655	22.54949866766107
68-69	22.57774159050112	26.99178403508291	27.948590629792207	22.48188374462376
70-71	22.619852484125204	26.534492506627828	28.04488634611588	22.800768663131084
72-73	22.695628897767605	26.622852931584656	27.98803344493403	22.693484725713706
74-75	22.470755245447467	26.949447721989838	27.891644133481336	22.688152899081352
76-77	22.64488248156745	26.80796924981166	27.765271910814192	22.7818763578067
78-79	22.628011407339375	26.8252898642776	27.64255187707422	22.904146851308806
80-81	22.64750985747039	26.919186261938666	27.53967269876017	22.893631181830777
82-83	22.76085768124011	26.82874030344854	27.611787606262094	22.79861440904925
84-85	22.791463347972932	26.83070167915377	27.549318664122968	22.828516308750338
86-87	22.60167680523108	26.96915941732842	27.63792671675172	22.791237060688783
88-89	22.725492886378266	26.939328049258705	27.540749350948317	22.794429713414715
90-91	22.781387591372653	26.930164505860482	27.53523863731513	22.753209265451737
92-93	22.81787382870194	26.940503301292363	27.50894684984097	22.732676020164725
94-95	22.661861833147572	26.91966744655569	27.54430576430618	22.874164955990555
96-97	22.766353353454626	27.0242970544932	27.644344466003535	22.565005126048636
98-99	23.852072250542335	29.009053056398532	27.74002475889684	19.39884993416229
100	30.81574809294586	40.06848118844222	29.115770718611923	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2293.0
1	2110.5
2	2617.0
3	3489.0
4	4195.5
5	5891.5
6	7667.0
7	9776.0
8	11926.0
9	12425.5
10	11510.0
11	10563.5
12	11109.5
13	13581.5
14	18865.0
15	25285.5
16	29729.0
17	31753.5
18	31096.5
19	28027.5
20	24440.5
21	21747.0
22	21289.0
23	23866.5
24	29303.5
25	38144.0
26	50999.5
27	65067.0
28	77368.0
29	91513.0
30	105740.0
31	118718.0
32	136109.5
33	156383.0
34	174972.5
35	193711.0
36	215156.0
37	231084.0
38	240842.0
39	248571.0
40	257482.0
41	265062.0
42	269438.0
43	276107.0
44	283574.5
45	289967.5
46	293167.5
47	294726.0
48	296304.5
49	297707.0
50	297686.5
51	295214.0
52	288903.5
53	278601.5
54	264068.5
55	247457.5
56	228022.0
57	205318.0
58	182956.0
59	159430.0
60	135458.0
61	110657.0
62	87077.0
63	67201.0
64	50409.5
65	37333.0
66	27207.5
67	20202.5
68	15132.5
69	10812.0
70	7597.0
71	5075.5
72	3245.5
73	2063.5
74	1365.5
75	885.0
76	571.0
77	355.0
78	217.0
79	138.5
80	95.0
81	65.5
82	47.5
83	33.5
84	18.5
85	10.0
86	7.0
87	6.0
88	3.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001940233116155622
3	0.0018974338562404245
4	0.0
5	0.0
6	3.85193339236778E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4980418453022123E-4
24-25	0.0010772345036071304
26-27	0.002647073471200911
28-29	0.0015988984731590272
30-31	0.0023655041178709977
32-33	0.0032670267799474765
34-35	0.0010134545798767163
36-37	0.0
38-39	5.089973646297877E-5
40-41	0.0
42-43	5.612490331377676E-4
44-45	0.0
46-47	0.0
48-49	1.4467454889143127E-4
50-51	0.005946391701821919
52-53	0.0
54-55	0.003015485497081245
56-57	0.04747364749927046
58-59	0.05961451199876888
60-61	0.037815003542834534
62-63	0.008414766535934442
64-65	0.0
66-67	0.0
68-69	5.906178664435819E-4
70-71	0.0025112380036067866
72-73	0.01005411847555358
74-75	0.004704333513112324
76-77	0.003945805860512578
78-79	0.003574296134645079
80-81	0.016463471966537222
82-83	0.05641005318176147
84-85	0.03559690489380179
86-87	0.009037980061405488
88-89	0.007809684294944471
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	52.0
20-21	177.0
22-23	419.0
24-25	760.0
26-27	2099.0
28-29	5559.0
30-31	16815.0
32-33	20945.0
34-35	28176.0
36-37	45175.0
38-39	52707.0
40-41	52190.0
42-43	58651.0
44-45	70375.0
46-47	71797.0
48-49	67912.0
50-51	60965.0
52-53	56125.0
54-55	60729.0
56-57	63515.0
58-59	66487.0
60-61	66254.0
62-63	62316.0
64-65	65878.0
66-67	69548.0
68-69	71986.0
70-71	71394.0
72-73	66184.0
74-75	65604.0
76-77	69210.0
78-79	72120.0
80-81	72355.0
82-83	67384.0
84-85	65523.0
86-87	67462.0
88-89	71203.0
90-91	72703.0
92-93	72000.0
94-95	79530.0
96-97	216829.0
98-99	1564547.0
100-101	3177807.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.87027344574433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.21250698355043	76.56360135920045
2	6.699146028788723	11.371167106325423
3	1.6779869003312509	4.272336212084706
4	0.6242108040730798	2.1190776651788092
5	0.2929985657120702	1.2433434195597148
6	0.1493727138746842	0.7606381843126426
7	0.09290432809105152	0.5519371010566483
8	0.060244904327175046	0.4090401203168042
9	0.040793195944827	0.31159167251069675
>10	0.1450396327489383	1.9438915141073032
>50	0.0035768657783673157	0.20391151467895724
>100	0.0011328470093006763	0.17223120521567256
>500	2.8743256704007737E-5	0.022247766272456095
>1k	5.748651340088575E-5	0.0549851591797112
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4266419971732516E-5	0.0	0.0	0.0
3	0.0	2.853283994346503E-5	0.0	0.0	0.0
4	0.0	1.5693061968905767E-4	0.0	0.0	0.0
5	0.0	1.5693061968905767E-4	0.0	1.4266419971732516E-5	0.0
6	0.0	1.711970396607902E-4	0.0	1.4266419971732516E-5	0.0
7	0.0	1.711970396607902E-4	0.0	1.4266419971732516E-5	0.0
8	0.0	1.854634596325227E-4	0.0	1.4266419971732516E-5	0.0
9	0.0	4.565254390954405E-4	0.0	1.4266419971732516E-5	1.4266419971732516E-5
10-11	0.0	4.565254390954405E-4	0.0	2.853283994346503E-5	4.279925991519754E-5
12-13	0.0	4.70791859067173E-4	0.0	2.853283994346503E-5	7.846530984452883E-5
14-15	0.0	6.91921368629027E-4	0.0	2.853283994346503E-5	1.1413135977386013E-4
16-17	0.0	7.061877886007595E-4	0.0	2.853283994346503E-5	1.2839777974559265E-4
18-19	0.0	7.703866784735558E-4	0.0	2.853283994346503E-5	1.2839777974559265E-4
20-21	0.0	8.345855683463522E-4	0.0	2.853283994346503E-5	1.2839777974559265E-4
22-23	0.0	0.0010414486579364735	0.0	2.853283994346503E-5	1.4266419971732517E-4
24-25	0.0	0.0012126456975972639	0.0	8.55985198303951E-5	1.5693061968905767E-4
26-27	0.0	0.0012697113774841938	0.0	1.5693061968905767E-4	1.5693061968905767E-4
28-29	0.0	0.0012697113774841938	0.0	6.847881586431608E-4	1.6406382967492391E-4
30-31	0.0	0.0014837076770601817	0.0	0.0021970286756468073	1.711970396607902E-4
32-33	0.0	0.0014837076770601817	0.0	0.005678035148749542	2.0686308959012146E-4
34-35	0.0	0.001747636446537233	0.0	0.009743964840693307	2.2826271954772025E-4
36-37	0.0	0.002018698426000151	0.0	0.016648912107011846	2.353959295335865E-4
38-39	0.0	0.0020543644759294824	0.0	0.03458180201147962	2.4252913951945278E-4
40-41	0.0	0.0022041618856326736	0.0	0.05437645972225848	2.4252913951945278E-4
42-43	0.0	0.0022612275655196036	0.0	0.06683817756756683	2.4252913951945278E-4
44-45	0.0	0.0023967585552510627	0.0	0.08085493518979403	2.4252913951945278E-4
46-47	0.0	0.0024823570750814577	0.0	0.09613427097951956	2.4252913951945278E-4
48-49	0.0	0.002489490285067324	0.0	0.11129947540947122	2.567955594911853E-4
50-51	0.0	0.0027177530046150443	0.0	0.12731353182774097	2.710619794629178E-4
52-53	0.0	0.002739152634572643	0.0	0.1651694772227332	2.710619794629178E-4
54-55	0.0	0.002767685474516108	0.0	0.2094025123450899	2.7819518944878404E-4
56-57	0.0	0.0028104847344313055	0.0	0.26763090545971613	2.853283994346503E-4
58-59	0.0	0.0028176179444171718	0.0	0.3123275992311541	2.853283994346503E-4
60-61	0.0	0.0028604172043323692	0.0	0.32705767785196793	2.853283994346503E-4
62-63	0.0	0.0029460157241627642	0.0	0.3443771116976512	2.853283994346503E-4
64-65	1.4266419971732516E-5	0.0032955430134702112	0.0	0.35368595072920667	2.924616094205166E-4
66-67	1.4266419971732516E-5	0.0033240758534136762	0.0	0.36142548356387155	2.9959481940638284E-4
68-69	1.4266419971732516E-5	0.00343107400320167	0.0	0.3689153540490311	2.9959481940638284E-4
70-71	1.4266419971732516E-5	0.0035024061030603324	0.0	0.3751925788365934	3.1386123937811533E-4
72-73	1.4266419971732516E-5	0.003523805733017931	0.0	0.3800859608868977	3.709269192650454E-4
74-75	1.4266419971732516E-5	0.0036379370927917916	0.0	0.38397356032919483	3.7806012925091167E-4
76-77	1.4266419971732516E-5	0.0037520684525656515	0.0	0.3876614298918877	3.851933392367779E-4
78-79	1.4266419971732516E-5	0.003823400552424314	0.0	0.39005105523715283	4.1372617918024297E-4
80-81	1.4266419971732516E-5	0.0038305337624101802	0.0	0.3908357083355981	4.1372617918024297E-4
82-83	1.4266419971732516E-5	0.0038590666023536457	0.0	0.3915989618040858	4.208593891661092E-4
84-85	1.4266419971732516E-5	0.003894732652282977	0.0	0.3919199562534498	4.2799259915197546E-4
86-87	1.4266419971732516E-5	0.003958931542155772	0.0	0.3920840200831247	4.2799259915197546E-4
88	1.4266419971732516E-5	0.004237126731604557	0.0	0.3921125529230682	4.2799259915197546E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9560	0.0	54.361523	1
GTATCAA	18195	0.0	39.330948	1
TCAACGC	23010	0.0	30.645256	4
ATCAACG	23370	0.0	30.191492	3
CAACGCA	23475	0.0	30.020432	5
TATCAAC	23930	0.0	29.852627	2
AACGCAG	24150	0.0	29.198648	6
ACGCAGA	27895	0.0	25.338531	7
CGCAGAG	28620	0.0	24.623678	8
GTACATG	27615	0.0	23.327515	1
TACATGG	27560	0.0	23.0709	2
ACATGGG	27250	0.0	22.612011	3
GTGGTAT	4065	0.0	22.403975	1
GCAGAGT	32760	0.0	21.550148	9
CATGGGG	19120	0.0	20.537493	4
TGGTATC	4100	0.0	20.072964	2
GAGTACT	18285	0.0	19.293549	12-13
CAGAGTA	31935	0.0	19.163647	10-11
AGAGTAC	30115	0.0	18.879162	10-11
GTACTTT	20050	0.0	17.49109	14-15
>>END_MODULE