FastQCFastQC Report
Fri 27 May 2016
SRR2139165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6490795
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86350.055.570961
GTATCAA167450.039.1506041
TCAACGC214550.030.2791044
ATCAACG215750.030.1305033
CAACGCA217800.029.7884945
TATCAAC222800.029.4434192
AACGCAG224950.028.9731126
ACGCAGA256250.025.3845047
CGCAGAG262950.024.673458
GTACATG233150.021.5597381
GCAGAGT302950.021.401759
TACATGG233300.021.401032
ACATGGG230050.021.0600663
GTGGTAT40950.019.9083521
GAGTACT183500.019.4160512-13
CATGGGG150450.019.3999714
AGAGTAC280600.019.2227310-11
CAGAGTA297250.018.94885610-11
TGGTATC40600.018.1031912
AGTACTT200150.017.5898412-13
Produced by FastQC (version 0.11.5)