##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6490795
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.06595247577531	18.0	18.0	30.0	18.0	33.0
2	30.455722141894793	31.0	29.0	33.0	27.0	33.0
3	31.657145542264082	33.0	31.0	33.0	29.0	33.0
4	32.56875251798893	33.0	33.0	33.0	31.0	34.0
5	32.9841746658152	33.0	33.0	34.0	32.0	34.0
6	36.97372078458802	38.0	38.0	38.0	35.0	38.0
7	37.076506036625716	38.0	38.0	38.0	36.0	38.0
8	37.06255227595387	38.0	38.0	38.0	36.0	38.0
9	37.26999450760654	38.0	38.0	38.0	36.0	38.0
10-11	37.30129930154935	38.0	38.0	38.0	37.0	38.0
12-13	37.35745066667488	38.0	38.0	38.0	37.0	38.0
14-15	37.26970856420516	38.0	38.0	38.0	36.5	38.0
16-17	37.34197706136151	38.0	38.0	38.0	37.0	38.0
18-19	37.3778935400055	38.0	38.0	38.0	37.0	38.0
20-21	37.39573122359387	38.0	38.0	38.0	37.0	38.0
22-23	37.41413185406037	38.0	38.0	38.0	37.0	38.0
24-25	37.42622933829181	38.0	38.0	38.0	37.0	38.0
26-27	37.394090487867715	38.0	38.0	38.0	37.0	38.0
28-29	37.37166909781708	38.0	38.0	38.0	37.0	38.0
30-31	37.369142885420516	38.0	38.0	38.0	37.0	38.0
32-33	37.35026308804187	38.0	38.0	38.0	37.0	38.0
34-35	37.3260366013009	38.0	38.0	38.0	37.0	38.0
36-37	37.302592248772584	38.0	38.0	38.0	37.0	38.0
38-39	37.297678665913196	38.0	38.0	38.0	37.0	38.0
40-41	37.28103963137824	38.0	38.0	38.0	37.0	38.0
42-43	37.23890842386788	38.0	38.0	38.0	37.0	38.0
44-45	37.145592707605374	38.0	38.0	38.0	37.0	38.0
46-47	37.15356606390971	38.0	38.0	38.0	37.0	38.0
48-49	37.101999917020734	38.0	38.0	38.0	36.0	38.0
50-51	37.044872179073444	38.0	38.0	38.0	36.0	38.0
52-53	37.0195097564451	38.0	38.0	38.0	36.0	38.0
54-55	36.988548270992155	38.0	38.0	38.0	36.0	38.0
56-57	36.94339397650822	38.0	38.0	38.0	36.0	38.0
58-59	36.928520185008296	38.0	38.0	38.0	36.0	38.0
60-61	36.93749040778063	38.0	38.0	38.0	36.0	38.0
62-63	36.94470287972127	38.0	38.0	38.0	36.0	38.0
64-65	36.90596132493444	38.0	38.0	38.0	36.0	38.0
66-67	36.934698243294264	38.0	38.0	38.0	36.0	38.0
68-69	36.92429376549047	38.0	38.0	38.0	36.0	38.0
70-71	36.93109712409398	38.0	38.0	38.0	36.0	38.0
72-73	36.915906809077185	38.0	38.0	38.0	35.5	38.0
74-75	36.91024326356387	38.0	38.0	38.0	35.0	38.0
76-77	36.89697057032026	38.0	38.0	38.0	35.0	38.0
78-79	36.87590649658361	38.0	38.0	38.0	35.0	38.0
80-81	36.84330651396165	38.0	38.0	38.0	35.0	38.0
82-83	36.807482397392654	38.0	38.0	38.0	35.0	38.0
84-85	36.78574841830364	38.0	38.0	38.0	35.0	38.0
86-87	36.778898531398596	38.0	38.0	38.0	35.0	38.0
88-89	36.7604583918139	38.0	38.0	38.0	35.0	38.0
90-91	36.738207372020725	38.0	38.0	38.0	35.0	38.0
92-93	36.71005363313182	38.0	38.0	38.0	34.0	38.0
94-95	36.681640913675466	38.0	38.0	38.0	34.0	38.0
96-97	36.683170928085076	38.0	38.0	38.0	34.0	38.0
98-99	36.692472253758034	38.0	38.0	38.0	34.0	38.0
100	35.23403126377899	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	9.0
20	31.0
21	103.0
22	412.0
23	1178.0
24	2976.0
25	6195.0
26	11337.0
27	19257.0
28	31201.0
29	45678.0
30	60024.0
31	76977.0
32	98290.0
33	131940.0
34	195207.0
35	349078.0
36	1084095.0
37	4376798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36850801789303	18.65333907479746	15.559927558950793	23.418225348358714
2	14.556478761052402	21.33354491909156	41.98027322294925	22.12970309690679
3	17.959958913414813	26.810352623907225	31.147307610000365	24.0823808526776
4	12.279250846776089	17.902691426859114	39.73186643546745	30.08619129089734
5	13.030607190644597	37.59725888739361	35.86947361609788	13.50266030586392
6	28.992347284635656	38.3962883899101	19.307576545641442	13.303787779812806
7	25.84087157274263	32.898281335337195	24.11119131015538	17.149655781764793
8	24.01269798229647	36.81022124408489	21.74115805536918	17.435922718249458
9	26.06786996045939	16.48878142045774	21.58649287182849	35.85685574725438
10-11	24.002175388376926	26.656326382207418	29.41108138525404	19.930416844161616
12-13	25.041377828139694	24.458097967968484	29.401329112997715	21.099195090894103
14-15	22.28706961165774	25.508146536749347	27.267461073720554	24.937322777872357
16-17	21.472870426503995	28.45738465010835	27.95069016969416	22.1190547536935
18-19	21.49053390224156	27.557394741322135	29.882364178810146	21.069707177626164
20-21	22.56149924584754	26.764724460884988	29.62530408752174	21.048472205745732
22-23	22.19465180462806	26.637840729403855	29.528957232710024	21.63855023325806
24-25	21.84012744060005	26.832249461107065	29.67426205431788	21.653361043975003
26-27	21.72717276813199	26.967883415279516	29.656388527380507	21.648555289207994
28-29	21.746151272205132	26.97581618944248	29.702394547274945	21.57563799107744
30-31	22.052373390383405	26.893043233809266	29.516643723634452	21.53793965217288
32-33	21.791592153427207	27.112680413295976	29.458312336608362	21.63741509666846
34-35	21.908333944713355	27.040296639002303	29.466619658910435	21.584749757373903
36-37	21.920790529409086	26.90814550095923	29.464874856076406	21.706189113555276
38-39	21.690413974935066	27.16427757123213	29.56338905495246	21.581919398880345
40-41	22.02206631506959	26.94736528210691	29.41062220975077	21.61994619307272
42-43	21.89486584325615	27.083275239539084	29.138814416285868	21.883044500918896
44-45	21.94593072080703	27.293954110543606	28.96885189680516	21.7912632718442
46-47	22.06339691145741	27.176537496046087	28.69442779283838	22.065637799658123
48-49	22.035811481865718	27.2277321145806	28.696878800586013	22.03957760296767
50-51	21.954706281498463	27.450733988066833	28.54281680201745	22.05174292841725
52-53	22.16568025885576	27.371989410688062	28.353840604546672	22.108489725909504
54-55	22.172782380492322	27.277714825037684	28.403627888989934	22.145874905480053
56-57	22.173051728304102	27.424012311174835	28.31016493187994	22.092771028641124
58-59	22.096109632423918	27.411004453731717	28.418407959773628	22.07447795407073
60-61	22.149820236500304	27.393922100009167	28.459167783121686	21.997089880368843
62-63	22.13309839250477	27.391812753437122	28.50702100574824	21.968067848309865
64-65	22.05697452240776	27.31912256046695	28.52478318508972	22.09911973203557
66-67	22.1634995572056	27.364711598692377	28.32791479387759	22.143874050224433
68-69	22.083827743504354	27.367129144825824	28.514725909945714	22.034317201724104
70-71	22.11735317474175	26.967401996688363	28.51258826159856	22.40265656697133
72-73	22.202762594714397	27.104204792423047	28.494937027917977	22.198095584944575
74-75	22.022909987242382	27.40381235363082	28.428006439109353	22.145271220017445
76-77	22.135608486068087	27.31232606229131	28.2911745442295	22.260890907411106
78-79	22.126553587173074	27.32577303309479	28.176538734532286	22.371134645199852
80-81	22.14123725650743	27.378270361543063	28.14295415949494	22.33753822245457
82-83	22.311105879492	27.294119654735116	28.22064629392738	22.1741281718455
84-85	22.407978501354155	27.298485236245845	28.06777127195714	22.225764990442855
86-87	22.10173340420652	27.44518185362442	28.180116306661603	22.272968435507465
88-89	22.293612639668787	27.3995838590056	28.05780388651513	22.248999614810483
90-91	22.336307406743693	27.47196724575285	28.06541879111354	22.12630655638992
92-93	22.412825408308173	27.447054036224184	27.95748292678917	22.182637628678474
94-95	22.202005769131087	27.483173367655855	28.01163568418035	22.303185179032702
96-97	22.33729115259112	27.609767469781172	28.061418138633886	21.99152323899382
98-99	23.333505115088048	29.553436150439587	28.32248245249376	18.790576281978606
100	29.916035976072063	40.482437028600735	29.601526995327205	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1582.0
1	1542.0
2	2115.5
3	2969.0
4	3828.0
5	5517.0
6	6995.5
7	8665.5
8	10547.5
9	11220.5
10	10710.5
11	10261.5
12	11134.0
13	13725.0
14	19200.5
15	25879.5
16	30759.0
17	33028.5
18	32424.5
19	29534.5
20	25905.0
21	23180.0
22	23124.0
23	26395.0
24	32716.0
25	42353.5
26	56448.0
27	71320.0
28	83842.0
29	97566.0
30	110739.5
31	123504.5
32	139910.0
33	159235.5
34	176498.5
35	193167.0
36	212440.5
37	226452.5
38	234890.0
39	240698.5
40	246913.0
41	251374.5
42	252039.0
43	255738.5
44	261550.5
45	264889.5
46	264508.5
47	264670.5
48	265630.5
49	264355.0
50	260169.0
51	253816.0
52	244464.5
53	232984.0
54	219144.0
55	203313.5
56	186631.0
57	168198.5
58	149432.5
59	128526.5
60	107046.0
61	85847.5
62	67111.0
63	51880.5
64	38190.0
65	27975.5
66	20514.0
67	15284.0
68	11515.5
69	8273.0
70	5916.5
71	4128.0
72	2729.0
73	1721.5
74	1089.0
75	716.5
76	467.5
77	280.5
78	184.5
79	126.0
80	77.5
81	52.0
82	42.5
83	29.5
84	17.5
85	9.0
86	5.0
87	4.0
88	4.5
89	5.0
90	3.5
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019258041580422736
3	0.0017255205256058773
4	0.0
5	0.0
6	4.159736981371311E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.4651538941650885E-4
24-25	0.0010323586660539285
26-27	0.0025735138881911464
28-29	0.0015801577814523082
30-31	0.0027162042650425823
32-33	0.0037274564285863427
34-35	9.771644425059709E-4
36-37	7.787545782981658E-6
38-39	3.917618745962404E-5
40-41	7.88888412049671E-6
42-43	5.640528036045993E-4
44-45	0.0
46-47	0.0
48-49	2.124055112041048E-4
50-51	0.006373601909606988
52-53	0.0
54-55	0.00294304947718948
56-57	0.047289222339401554
58-59	0.058764282083251476
60-61	0.03900675901458625
62-63	0.008770316302720313
64-65	0.0
66-67	0.0
68-69	7.445813317850826E-4
70-71	0.002820844791314628
72-73	0.010048190056014076
74-75	0.004742314925834178
76-77	0.0038470632305559038
78-79	0.00404195109807127
80-81	0.01667575963305283
82-83	0.055063267034846944
84-85	0.036008258677843366
86-87	0.009411443026838958
88-89	0.008208283129843414
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	194.0
22-23	462.0
24-25	624.0
26-27	1836.0
28-29	4819.0
30-31	13477.0
32-33	16975.0
34-35	23365.0
36-37	36679.0
38-39	43680.0
40-41	43361.0
42-43	49535.0
44-45	59416.0
46-47	61300.0
48-49	57539.0
50-51	52196.0
52-53	48965.0
54-55	53822.0
56-57	56976.0
58-59	56272.0
60-61	55255.0
62-63	52076.0
64-65	54466.0
66-67	58898.0
68-69	60613.0
70-71	60208.0
72-73	55902.0
74-75	55446.0
76-77	58964.0
78-79	61806.0
80-81	61658.0
82-83	57250.0
84-85	55573.0
86-87	57164.0
88-89	61137.0
90-91	61784.0
92-93	61123.0
94-95	68586.0
96-97	195122.0
98-99	1467293.0
100-101	3088942.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.40473201828935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.43325080703937	77.23427550717946
2	6.677379865453538	11.40559675986761
3	1.593445342573176	4.082633174047602
4	0.553276806535567	1.8900982957642012
5	0.2789942712572571	1.1913715485681984
6	0.15095760018586282	0.7735496033998608
7	0.08052194466910893	0.48138685742397397
8	0.05141974597499307	0.3513191700354237
9	0.03731396222259082	0.28681100497448475
>10	0.13892882696243491	1.8852303210569787
>50	0.0035849931572384643	0.19932013004907134
>100	7.763050370684017E-4	0.10265602089106962
>500	8.971737098435688E-5	0.04939621445432441
>1k	5.981156080772905E-5	0.06635539228770748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.540643326433819E-5	0.0	0.0	0.0
3	0.0	1.540643326433819E-5	0.0	0.0	0.0
4	0.0	1.6947076590772007E-4	0.0	0.0	0.0
5	0.0	2.0028363243639646E-4	0.0	1.540643326433819E-5	0.0
6	0.0	2.0028363243639646E-4	0.0	1.540643326433819E-5	0.0
7	0.0	2.0028363243639646E-4	0.0	3.081286652867638E-5	0.0
8	0.0	2.46502932229411E-4	0.0	3.081286652867638E-5	0.0
9	0.0	4.159736981371311E-4	0.0	4.621929979301457E-5	0.0
10-11	0.0	4.159736981371311E-4	0.0	5.3922516425183666E-5	0.0
12-13	0.0	4.159736981371311E-4	0.0	6.932894968952186E-5	0.0
14-15	0.0	4.621929979301457E-4	0.0	7.703216632169095E-5	3.081286652867638E-5
16-17	0.0	4.698962145623148E-4	0.0	9.243859958602914E-5	3.081286652867638E-5
18-19	0.0	5.007090810909911E-4	0.0	9.243859958602914E-5	3.081286652867638E-5
20-21	0.0	5.238187309874984E-4	0.0	9.243859958602914E-5	3.081286652867638E-5
22-23	1.540643326433819E-5	6.008508973091894E-4	0.0	1.232514661147055E-4	3.8516083160845474E-5
24-25	1.540643326433819E-5	6.316637638378658E-4	0.0	2.0028363243639649E-4	4.621929979301457E-5
26-27	1.540643326433819E-5	6.701798469987112E-4	0.0	2.46502932229411E-4	4.621929979301457E-5
28-29	1.540643326433819E-5	6.778830636308803E-4	0.0	4.621929979301457E-4	4.621929979301457E-5
30-31	1.540643326433819E-5	7.703216632169095E-4	0.0	0.0010938567617680116	4.621929979301457E-5
32-33	1.540643326433819E-5	7.703216632169095E-4	0.0	0.0027192354711556904	4.621929979301457E-5
34-35	1.540643326433819E-5	8.165409630099241E-4	0.0	0.004621929979301457	4.621929979301457E-5
36-37	1.540643326433819E-5	8.704634794351077E-4	0.0	0.0082501450130531	4.621929979301457E-5
38-39	1.540643326433819E-5	8.781666960672768E-4	0.0	0.014089183220237275	4.621929979301457E-5
40-41	1.540643326433819E-5	9.551988623889677E-4	0.0	0.020036066460271815	4.621929979301457E-5
42-43	1.540643326433819E-5	9.93714945549813E-4	0.0	0.025913620750616834	4.621929979301457E-5
44-45	1.540643326433819E-5	0.0011169664116645187	0.0	0.03273867068671865	4.621929979301457E-5
46-47	1.540643326433819E-5	0.0012017017946183788	0.0	0.03949439167313095	7.703216632169095E-5
48-49	1.540643326433819E-5	0.0012017017946183788	0.0	0.04661216384125519	1.0784503285036733E-4
50-51	1.540643326433819E-5	0.0012556243110435623	0.0	0.05402265824140186	1.1554824948253642E-4
52-53	1.540643326433819E-5	0.0012633275276757315	0.0	0.060978662860250554	1.3865789937904372E-4
54-55	1.540643326433819E-5	0.0012633275276757315	0.0	0.06762653881381248	1.540643326433819E-4
56-57	1.540643326433819E-5	0.0012633275276757315	0.0	0.07484445279815492	1.540643326433819E-4
58-59	1.540643326433819E-5	0.0012633275276757315	0.0	0.08240901153094499	1.540643326433819E-4
60-61	1.540643326433819E-5	0.0012787339609400697	0.0	0.08961922229865525	1.540643326433819E-4
62-63	1.540643326433819E-5	0.0012864371775722387	0.0	0.0964134593682284	1.540643326433819E-4
64-65	1.540643326433819E-5	0.0014019854270547753	0.0	0.10292267742241128	1.6176754927555098E-4
66-67	1.540643326433819E-5	0.0014019854270547753	0.0	0.1096938048420879	1.7717398253988916E-4
68-69	1.540643326433819E-5	0.0014019854270547753	0.0	0.11628775827922466	1.8487719917205828E-4
70-71	1.540643326433819E-5	0.0014019854270547753	0.0	0.12168000992174302	2.0028363243639646E-4
72-73	1.540643326433819E-5	0.0014096886436869445	0.0	0.12626342381788364	2.3109649896507284E-4
74-75	1.540643326433819E-5	0.001463611160112128	0.0	0.1295835101863485	2.3109649896507284E-4
76-77	1.540643326433819E-5	0.0015021272432729736	0.0	0.13274182900553785	2.3109649896507284E-4
78-79	1.540643326433819E-5	0.0015098304599051426	0.0	0.1347677749797983	2.3109649896507284E-4
80-81	1.540643326433819E-5	0.0015098304599051426	0.0	0.13546106447669354	2.3109649896507284E-4
82-83	1.540643326433819E-5	0.00153294010980165	0.0	0.13622368292327827	2.619093654937492E-4
84-85	1.540643326433819E-5	0.0016099722761233407	0.0	0.1365626244550937	2.619093654937492E-4
86-87	1.540643326433819E-5	0.0016638947925485244	0.0	0.13673209522100144	2.619093654937492E-4
88	1.540643326433819E-5	0.00178714625866323	0.0	0.1367629080875301	2.619093654937492E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8635	0.0	55.57096	1
GTATCAA	16745	0.0	39.150604	1
TCAACGC	21455	0.0	30.279104	4
ATCAACG	21575	0.0	30.130503	3
CAACGCA	21780	0.0	29.788494	5
TATCAAC	22280	0.0	29.443419	2
AACGCAG	22495	0.0	28.973112	6
ACGCAGA	25625	0.0	25.384504	7
CGCAGAG	26295	0.0	24.67345	8
GTACATG	23315	0.0	21.559738	1
GCAGAGT	30295	0.0	21.40175	9
TACATGG	23330	0.0	21.40103	2
ACATGGG	23005	0.0	21.060066	3
GTGGTAT	4095	0.0	19.908352	1
GAGTACT	18350	0.0	19.41605	12-13
CATGGGG	15045	0.0	19.399971	4
AGAGTAC	28060	0.0	19.22273	10-11
CAGAGTA	29725	0.0	18.948856	10-11
TGGTATC	4060	0.0	18.103191	2
AGTACTT	20015	0.0	17.58984	12-13
>>END_MODULE