FastQCFastQC Report
Fri 27 May 2016
SRR2139164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6190356
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.052.960661
GTATCAA154050.040.15621
TCAACGC190900.032.0033454
ATCAACG194300.031.4438323
CAACGCA195300.031.2605725
TATCAAC198150.031.1333222
AACGCAG199800.030.6415626
ACGCAGA230200.026.5212427
CGCAGAG235750.025.7166678
GCAGAGT275750.021.9862379
GTACATG201000.021.0319711
GTGGTAT38800.021.0243431
TGGTATC35950.020.4456122
GAGTACT161300.020.07072312-13
TACATGG205850.020.0410862
ACATGGG203750.019.9555533
AGAGTAC252400.019.30707410-11
CAGAGTA269550.018.93768110-11
CATGGGG143100.017.7840064
AGTACTT176350.017.55078312-13
Produced by FastQC (version 0.11.5)