##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6190356
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.777135272995608	18.0	18.0	30.0	18.0	33.0
2	30.325817610489608	31.0	29.0	33.0	27.0	33.0
3	31.55969123585138	33.0	31.0	33.0	29.0	33.0
4	32.53543592646368	33.0	33.0	33.0	31.0	34.0
5	32.960926802917314	33.0	33.0	34.0	32.0	34.0
6	36.93255945215429	38.0	37.0	38.0	35.0	38.0
7	37.00959702479147	38.0	38.0	38.0	35.0	38.0
8	36.99224503404974	38.0	38.0	38.0	35.0	38.0
9	37.22048748084924	38.0	38.0	38.0	36.0	38.0
10-11	37.2645674982182	38.0	38.0	38.0	36.0	38.0
12-13	37.3246315720776	38.0	38.0	38.0	37.0	38.0
14-15	37.23322592432487	38.0	38.0	38.0	36.5	38.0
16-17	37.30542354914644	38.0	38.0	38.0	36.5	38.0
18-19	37.353696621002086	38.0	38.0	38.0	37.0	38.0
20-21	37.373260190434806	38.0	38.0	38.0	37.0	38.0
22-23	37.397051466779246	38.0	38.0	38.0	37.0	38.0
24-25	37.40987694426802	38.0	38.0	38.0	37.0	38.0
26-27	37.3779703952527	38.0	38.0	38.0	37.0	38.0
28-29	37.35564085539746	38.0	38.0	38.0	37.0	38.0
30-31	37.35237405236923	38.0	38.0	38.0	37.0	38.0
32-33	37.33695285302268	38.0	38.0	38.0	37.0	38.0
34-35	37.313544045726914	38.0	38.0	38.0	37.0	38.0
36-37	37.290509217877954	38.0	38.0	38.0	37.0	38.0
38-39	37.282986129684566	38.0	38.0	38.0	37.0	38.0
40-41	37.26850596103016	38.0	38.0	38.0	37.0	38.0
42-43	37.226429815221394	38.0	38.0	38.0	37.0	38.0
44-45	37.13429737273584	38.0	38.0	38.0	36.0	38.0
46-47	37.146326896413186	38.0	38.0	38.0	36.0	38.0
48-49	37.09633842562426	38.0	38.0	38.0	36.0	38.0
50-51	37.03484411216042	38.0	38.0	38.0	36.0	38.0
52-53	37.00434274267993	38.0	38.0	38.0	36.0	38.0
54-55	36.968800931435766	38.0	38.0	38.0	36.0	38.0
56-57	36.91919287622991	38.0	38.0	38.0	36.0	38.0
58-59	36.90124243962919	38.0	38.0	38.0	36.0	38.0
60-61	36.90973064355585	38.0	38.0	38.0	36.0	38.0
62-63	36.913565261857016	38.0	38.0	38.0	36.0	38.0
64-65	36.87277929591153	38.0	38.0	38.0	35.0	38.0
66-67	36.89776465957735	38.0	38.0	38.0	35.0	38.0
68-69	36.88309575341152	38.0	38.0	38.0	35.0	38.0
70-71	36.8855461141708	38.0	38.0	38.0	35.0	38.0
72-73	36.86943081346329	38.0	38.0	38.0	35.0	38.0
74-75	36.859300833797626	38.0	38.0	38.0	35.0	38.0
76-77	36.84221229285776	38.0	38.0	38.0	35.0	38.0
78-79	36.81993919191919	38.0	38.0	38.0	35.0	38.0
80-81	36.7851381000343	38.0	38.0	38.0	35.0	38.0
82-83	36.74656571975931	38.0	38.0	38.0	35.0	38.0
84-85	36.71831472005567	38.0	38.0	38.0	34.0	38.0
86-87	36.70794421643344	38.0	38.0	38.0	34.0	38.0
88-89	36.68455343908688	38.0	38.0	38.0	34.0	38.0
90-91	36.66162019452482	38.0	38.0	38.0	34.0	38.0
92-93	36.630798371241454	38.0	38.0	38.0	34.0	38.0
94-95	36.60131356504763	38.0	38.0	38.0	34.0	38.0
96-97	36.59763649489173	38.0	38.0	38.0	34.0	38.0
98-99	36.60413587022573	38.0	38.0	38.0	34.0	38.0
100	35.09017171071601	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	10.0
20	18.0
21	100.0
22	411.0
23	1215.0
24	2881.0
25	5901.0
26	10900.0
27	18337.0
28	29180.0
29	43079.0
30	57387.0
31	74296.0
32	96215.0
33	130638.0
34	195954.0
35	355895.0
36	1107566.0
37	4060365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22831449435218	18.621336155788132	15.801482176469333	23.34886717339035
2	14.516434142514045	21.435068970525116	41.72189731179	22.32659957517084
3	17.87024007281131	26.771355004089497	31.372544585054523	23.98586033804467
4	12.406624887688169	17.99005355751868	39.50809598287917	30.095225571913982
5	13.0912987879857	37.57947038910201	35.95642964637252	13.372801176539767
6	29.241452639373783	38.271986058259884	19.276946696881524	13.20961460548481
7	25.92519719382859	32.93560176506812	24.073591244186925	17.065609796916366
8	24.12184371948883	36.77609171427297	21.70965611670799	17.392408449530205
9	26.045949538281803	16.55323215659972	21.607626443454947	35.79319186166353
10-11	23.994605479878704	26.54752004569689	29.507495207060792	19.950379267363623
12-13	25.019288066792928	24.42794889340775	29.46276433859377	21.089998701205552
14-15	22.258509849837395	25.542505148330726	27.18185351537133	25.017131486460553
16-17	21.57703046480687	28.542308390664445	27.83673507630256	22.043926068226124
18-19	21.613425786820663	27.70230823558451	29.683906709081025	21.000359268513797
20-21	22.538100955567966	26.88100208431016	29.48537610672185	21.095520853400018
22-23	22.275654500988963	26.774385817562735	29.325694334894138	21.624265346554168
24-25	21.93717796002128	26.932401432522035	29.467103353133613	21.66331725432307
26-27	21.836233119209925	27.061179844193045	29.43183629532759	21.670750741269437
28-29	21.84382249982341	27.03273482446809	29.472891076968434	21.650551598740062
30-31	22.09163211884429	26.954501723372985	29.346221766438706	21.60764439134401
32-33	21.879544674625315	27.166253525789934	29.26576906585628	21.68843273372847
34-35	22.00662448131937	27.129437488061708	29.29400911170989	21.569928918909035
36-37	22.01343116507499	26.971136655062487	29.28979071190332	21.7256414679592
38-39	21.831227274458232	27.214792399016197	29.366603285740737	21.587377040784837
40-41	22.08856654624798	27.009342305153332	29.236317104453686	21.665774044145003
42-43	21.977291813424962	27.148784623075645	28.987461503357544	21.886462060141852
44-45	22.048042957025633	27.275336801986246	28.849208945883703	21.827411295104422
46-47	22.12799428708207	27.201075213632	28.632720541266167	22.03820995801976
48-49	22.08761180328764	27.303838764388118	28.568687571876588	22.039861860447658
50-51	22.075513227644343	27.50592299920338	28.411319704627036	22.00724406852523
52-53	22.255044909343717	27.373950534344367	28.336613193500536	22.03439136281138
54-55	22.258734005098095	27.26544087511764	28.33784972940947	22.13797539037479
56-57	22.157585551426344	27.456858931127265	28.330996967206023	22.054558550240365
58-59	22.150970106257784	27.400844396885045	28.36249885678313	22.085686640074048
60-61	22.217245904111234	27.383486841278586	28.41818005575471	21.981087198855466
62-63	22.155631283666295	27.460076510894677	28.44129778174882	21.942994423690212
64-65	22.154921886965983	27.3028407378408	28.4569058888412	22.08533148635201
66-67	22.184337065805845	27.335655822090544	28.39411556339238	22.08589154871123
68-69	22.139374762066552	27.346512225918346	28.505794691204716	22.008318320810393
70-71	22.11553922908751	27.054676950111094	28.50122215128888	22.32856166951251
72-73	22.255467071034733	27.11068427252431	28.466968417541295	22.166880238899665
74-75	22.087674165936313	27.416723954573165	28.369444039313912	22.12615784017661
76-77	22.221897517235785	27.28671793285322	28.24380523364873	22.247579316262257
78-79	22.16460970455368	27.291127516918955	28.205547652290175	22.338715126237187
80-81	22.19021446460863	27.415214511569015	28.117445248395217	22.277125775427145
82-83	22.324757832727002	27.324934288842368	28.190319067647874	22.159988810782757
84-85	22.34845868171405	27.349163623700758	28.056852662277038	22.24552503230816
86-87	22.21192688430782	27.418823221453735	28.11783393389849	22.25141596033995
88-89	22.312898331367563	27.387802106945173	28.045374329141044	22.25392523254622
90-91	22.39544690392826	27.46351271950233	27.9778148906469	22.163225485922506
92-93	22.390297388619125	27.433959730920233	27.985750155054827	22.189992725405812
94-95	22.221264285849355	27.454321124558902	28.01962822311314	22.304786366478606
96-97	22.384056991944128	27.55947630527253	28.018790954379597	22.03767574840375
98-99	23.40675849804368	29.498533493550777	28.240250991668457	18.854457016737086
100	29.885510687573664	40.46383019998592	29.650659112440426	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1583.0
1	1460.5
2	1842.5
3	2538.5
4	3117.5
5	4378.0
6	5670.0
7	7419.5
8	9208.5
9	9767.0
10	9341.5
11	8813.5
12	9379.0
13	11474.5
14	16003.0
15	21405.5
16	25296.0
17	27373.5
18	27084.0
19	25006.0
20	22798.5
21	21133.0
22	21347.0
23	24537.0
24	30451.0
25	39494.5
26	51235.0
27	63725.5
28	76754.5
29	91526.5
30	106152.5
31	119542.0
32	134757.0
33	152093.0
34	167109.5
35	181418.5
36	198096.5
37	211063.0
38	220940.5
39	228074.5
40	234781.5
41	241606.0
42	244189.5
43	247337.0
44	252041.5
45	254728.5
46	254558.0
47	254670.5
48	254471.5
49	251877.0
50	247654.5
51	241822.0
52	233158.5
53	221654.5
54	207816.0
55	192583.0
56	175393.0
57	156731.0
58	138285.0
59	118186.0
60	98426.0
61	79957.5
62	62987.5
63	48263.0
64	35578.5
65	26211.5
66	19137.0
67	14159.5
68	10733.0
69	7907.5
70	5713.0
71	3993.5
72	2732.0
73	1812.0
74	1146.5
75	720.0
76	464.5
77	275.0
78	162.0
79	109.5
80	72.0
81	44.5
82	24.5
83	12.5
84	7.5
85	5.5
86	5.0
87	4.5
88	3.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016638784586863824
3	0.0017608034174448125
4	3.230831958614335E-5
5	0.0
6	3.8769983503372015E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.6962599406890596E-4
24-25	0.0010420524717848138
26-27	0.0028437309265028853
28-29	0.0018992138143802168
30-31	0.0024836276755728585
32-33	0.003153482677084671
34-35	0.0010078863858445934
36-37	8.160079211520921E-6
38-39	4.1035630992176724E-5
40-41	1.651984653062573E-5
42-43	5.071475378652148E-4
44-45	0.0
46-47	0.0
48-49	1.4503266946192965E-4
50-51	0.006177744906285847
52-53	0.0
54-55	0.002839962023153992
56-57	0.04625037510129387
58-59	0.05743921401815577
60-61	0.0377359641682396
62-63	0.008479775373736823
64-65	0.0
66-67	0.0
68-69	5.20985481251035E-4
70-71	0.0027723067791452613
72-73	0.01015626698149196
74-75	0.004791064549629393
76-77	0.00370601525949364
78-79	0.0037741298650709465
80-81	0.015875032063907384
82-83	0.052089890784096246
84-85	0.03489153940744959
86-87	0.009363879436890156
88-89	0.008320918117938239
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	161.0
22-23	358.0
24-25	492.0
26-27	1679.0
28-29	4298.0
30-31	12129.0
32-33	15093.0
34-35	21067.0
36-37	33010.0
38-39	39244.0
40-41	38607.0
42-43	43663.0
44-45	53083.0
46-47	53872.0
48-49	50650.0
50-51	45773.0
52-53	43615.0
54-55	47616.0
56-57	49963.0
58-59	50376.0
60-61	49210.0
62-63	46523.0
64-65	49407.0
66-67	52716.0
68-69	53556.0
70-71	53860.0
72-73	49852.0
74-75	50187.0
76-77	52653.0
78-79	55345.0
80-81	55211.0
82-83	52047.0
84-85	50409.0
86-87	51766.0
88-89	55105.0
90-91	56838.0
92-93	55939.0
94-95	63874.0
96-97	190029.0
98-99	1430636.0
100-101	3010412.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.63051564103296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70456031129017	78.57782830760239
2	6.680701491776396	11.575052301528146
3	1.4979774012164908	3.893116640579973
4	0.5275546844059208	1.828093373557093
5	0.2269130307469499	0.9828796429638917
6	0.11816781002334106	0.6142162988696195
7	0.06907194138306641	0.4188616528839572
8	0.044174613236252824	0.30614956183198255
9	0.030059845394818874	0.23436899159785998
>10	0.09775383156060201	1.3121551633383695
>50	0.002561439125776037	0.15494906832034497
>100	4.459709182913537E-4	0.07035252878613152
>500	5.76289219208259E-5	0.03197646814022498
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.8462479379215026E-5	0.0	0.0	0.0
3	0.0	4.8462479379215026E-5	0.0	0.0	0.0
4	0.0	1.292332783445734E-4	0.0	0.0	0.0
5	0.0	1.292332783445734E-4	0.0	0.0	0.0
6	0.0	1.292332783445734E-4	0.0	0.0	0.0
7	0.0	1.6154159793071676E-4	0.0	0.0	0.0
8	0.0	1.938499175168601E-4	0.0	0.0	0.0
9	0.0	3.876998350337202E-4	0.0	0.0	0.0
10-11	0.0	3.876998350337202E-4	0.0	0.0	0.0
12-13	1.6154159793071676E-5	3.9577691493025604E-4	0.0	8.077079896535838E-6	0.0
14-15	1.6154159793071676E-5	5.088560334817578E-4	0.0	1.6154159793071676E-5	0.0
16-17	1.6154159793071676E-5	5.330872731713653E-4	0.0	1.6154159793071676E-5	0.0
18-19	1.6154159793071676E-5	5.734726726540444E-4	0.0	1.6154159793071676E-5	0.0
20-21	1.6154159793071676E-5	6.300122319297954E-4	0.0	3.230831958614335E-5	8.077079896535838E-6
22-23	2.4231239689607516E-5	7.188601107916896E-4	0.0	3.230831958614335E-5	3.230831958614335E-5
24-25	4.038539948267919E-5	7.996309097570479E-4	0.0	4.038539948267919E-5	4.8462479379215026E-5
26-27	4.8462479379215026E-5	8.400163092397271E-4	0.0	8.077079896535838E-5	4.8462479379215026E-5
28-29	4.8462479379215026E-5	8.48093389136263E-4	0.0	2.98851956171826E-4	4.8462479379215026E-5
30-31	4.8462479379215026E-5	9.530954277912288E-4	0.0	9.45018347894693E-4	4.8462479379215026E-5
32-33	4.8462479379215026E-5	9.530954277912288E-4	0.0	0.0026573592859602904	8.077079896535838E-5
34-35	6.46166391722867E-5	0.0011550224252046249	0.0	0.00483009377812843	8.077079896535838E-5
36-37	8.077079896535838E-5	0.0013731035824110925	0.0	0.008157850695501196	8.077079896535838E-5
38-39	8.077079896535838E-5	0.001389257742204164	0.0	0.0136583421050421	8.077079896535838E-5
40-41	8.077079896535838E-5	0.0015184910205487375	0.0	0.01901344607644536	8.077079896535838E-5
42-43	8.077079896535838E-5	0.00159118473961756	0.0	0.023665844096850006	8.077079896535838E-5
44-45	8.077079896535838E-5	0.0017931117370309558	0.0	0.02923902922545973	8.077079896535838E-5
46-47	8.077079896535838E-5	0.0019223450153755292	0.0	0.03555530570455076	8.077079896535838E-5
48-49	8.077079896535838E-5	0.0019223450153755292	0.0	0.04175042598519374	8.077079896535838E-5
50-51	8.077079896535838E-5	0.002083886613306246	0.0	0.04807477954418131	8.077079896535838E-5
52-53	8.077079896535838E-5	0.002083886613306246	0.0	0.0546091371804788	8.077079896535838E-5
54-55	8.077079896535838E-5	0.0021081178529958537	0.0	0.06075579498174257	8.077079896535838E-5
56-57	8.077079896535838E-5	0.0021323490926854613	0.0	0.06703168606135092	8.884787886189421E-5
58-59	8.077079896535838E-5	0.0021323490926854613	0.0	0.07342065625951076	9.692495875843005E-5
60-61	8.077079896535838E-5	0.0021727344921681404	0.0	0.07970462441901564	9.692495875843005E-5
62-63	8.077079896535838E-5	0.002237351131340427	0.0	0.0858270509805898	9.692495875843005E-5
64-65	8.077079896535838E-5	0.0024392781287538227	0.0	0.09165062558599214	9.692495875843005E-5
66-67	8.077079896535838E-5	0.0024473552086503587	0.0	0.09820921446197925	9.692495875843005E-5
68-69	8.077079896535838E-5	0.002576588486994932	0.0	0.10461433881993216	9.692495875843005E-5
70-71	8.077079896535838E-5	0.002641205126167219	0.0	0.10992098031195621	9.692495875843005E-5
72-73	8.077079896535838E-5	0.002649282206063755	0.0	0.114379528414844	1.292332783445734E-4
74-75	8.077079896535838E-5	0.002730053005029113	0.0	0.1176749770126306	1.292332783445734E-4
76-77	8.077079896535838E-5	0.0028431321235806144	0.0	0.1208492694119692	1.292332783445734E-4
78-79	8.077079896535838E-5	0.0028915946029598296	0.0	0.12294931018506852	1.292332783445734E-4
80-81	8.077079896535838E-5	0.0028915946029598296	0.0	0.12357932241699832	1.3731035824110925E-4
82-83	9.692495875843005E-5	0.0028996716828563656	0.0	0.12401548473141125	1.4538743813764508E-4
84-85	1.2115619844803757E-4	0.0029077487627529016	0.0	0.1241851034092385	1.4538743813764508E-4
86-87	1.292332783445734E-4	0.0029723654019251883	0.0	0.12430625960768654	1.4538743813764508E-4
88	1.292332783445734E-4	0.003117752840062833	0.0	0.12432241376747961	1.4538743813764508E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7910	0.0	52.96066	1
GTATCAA	15405	0.0	40.1562	1
TCAACGC	19090	0.0	32.003345	4
ATCAACG	19430	0.0	31.443832	3
CAACGCA	19530	0.0	31.260572	5
TATCAAC	19815	0.0	31.133322	2
AACGCAG	19980	0.0	30.641562	6
ACGCAGA	23020	0.0	26.521242	7
CGCAGAG	23575	0.0	25.716667	8
GCAGAGT	27575	0.0	21.986237	9
GTACATG	20100	0.0	21.031971	1
GTGGTAT	3880	0.0	21.024343	1
TGGTATC	3595	0.0	20.445612	2
GAGTACT	16130	0.0	20.070723	12-13
TACATGG	20585	0.0	20.041086	2
ACATGGG	20375	0.0	19.955553	3
AGAGTAC	25240	0.0	19.307074	10-11
CAGAGTA	26955	0.0	18.937681	10-11
CATGGGG	14310	0.0	17.784006	4
AGTACTT	17635	0.0	17.550783	12-13
>>END_MODULE