FastQCFastQC Report
Fri 27 May 2016
SRR2139163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6491338
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78600.052.0004041
GTATCAA150150.039.1089941
TCAACGC184450.031.0548134
ATCAACG186950.030.6848873
CAACGCA190000.030.1476845
AACGCAG194200.029.539346
TATCAAC196500.029.4956652
ACGCAGA223600.025.6743287
CGCAGAG232050.024.6845938
GTACATG210250.023.049551
TACATGG211400.022.6433772
ACATGGG209450.022.2873883
GCAGAGT267400.021.3896059
TGGTATC38450.020.1793382
GTGGTAT40650.020.0259381
CATGGGG141100.019.6518294
GAGTACT165050.019.2277512-13
AGAGTAC245200.019.01196510-11
CAGAGTA262350.018.62567710-11
GTACTTT184400.017.19879214-15
Produced by FastQC (version 0.11.5)