##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6491338
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.89946772144664	18.0	18.0	30.0	18.0	33.0
2	30.385854041185347	31.0	29.0	33.0	27.0	33.0
3	31.606258524821847	33.0	31.0	33.0	29.0	33.0
4	32.54754258675176	33.0	33.0	33.0	31.0	34.0
5	32.967783067219735	33.0	33.0	34.0	32.0	34.0
6	36.9568139881177	38.0	38.0	38.0	35.0	38.0
7	37.0408550594654	38.0	38.0	38.0	36.0	38.0
8	37.026903698436286	38.0	38.0	38.0	36.0	38.0
9	37.24861808151108	38.0	38.0	38.0	36.0	38.0
10-11	37.283017846243716	38.0	38.0	38.0	36.5	38.0
12-13	37.340981088952695	38.0	38.0	38.0	37.0	38.0
14-15	37.248518872380394	38.0	38.0	38.0	36.5	38.0
16-17	37.31842672496795	38.0	38.0	38.0	37.0	38.0
18-19	37.363765913899414	38.0	38.0	38.0	37.0	38.0
20-21	37.38264637380083	38.0	38.0	38.0	37.0	38.0
22-23	37.403930984568774	38.0	38.0	38.0	37.0	38.0
24-25	37.4173067963094	38.0	38.0	38.0	37.0	38.0
26-27	37.384703575437875	38.0	38.0	38.0	37.0	38.0
28-29	37.360467751131544	38.0	38.0	38.0	37.0	38.0
30-31	37.36064732847365	38.0	38.0	38.0	37.0	38.0
32-33	37.343237166267755	38.0	38.0	38.0	37.0	38.0
34-35	37.31856837845616	38.0	38.0	38.0	37.0	38.0
36-37	37.29425415269209	38.0	38.0	38.0	37.0	38.0
38-39	37.29120023506852	38.0	38.0	38.0	37.0	38.0
40-41	37.27616016407845	38.0	38.0	38.0	37.0	38.0
42-43	37.236451381544924	38.0	38.0	38.0	37.0	38.0
44-45	37.14615946259884	38.0	38.0	38.0	36.0	38.0
46-47	37.1590740541378	38.0	38.0	38.0	37.0	38.0
48-49	37.110017669010944	38.0	38.0	38.0	36.0	38.0
50-51	37.05323004307543	38.0	38.0	38.0	36.0	38.0
52-53	37.02435441332267	38.0	38.0	38.0	36.0	38.0
54-55	36.99171008833885	38.0	38.0	38.0	36.0	38.0
56-57	36.94485082148	38.0	38.0	38.0	36.0	38.0
58-59	36.92713303024678	38.0	38.0	38.0	36.0	38.0
60-61	36.93575722022432	38.0	38.0	38.0	36.0	38.0
62-63	36.93693713331612	38.0	38.0	38.0	36.0	38.0
64-65	36.89774002524686	38.0	38.0	38.0	36.0	38.0
66-67	36.922248899914045	38.0	38.0	38.0	36.0	38.0
68-69	36.90897344436797	38.0	38.0	38.0	35.0	38.0
70-71	36.91460481433805	38.0	38.0	38.0	35.0	38.0
72-73	36.89554144674231	38.0	38.0	38.0	35.0	38.0
74-75	36.88856690937122	38.0	38.0	38.0	35.0	38.0
76-77	36.87255028392987	38.0	38.0	38.0	35.0	38.0
78-79	36.84705343666362	38.0	38.0	38.0	35.0	38.0
80-81	36.81269120845732	38.0	38.0	38.0	35.0	38.0
82-83	36.77511119466883	38.0	38.0	38.0	35.0	38.0
84-85	36.7501154522094	38.0	38.0	38.0	35.0	38.0
86-87	36.74206880189958	38.0	38.0	38.0	35.0	38.0
88-89	36.72210346117487	38.0	38.0	38.0	34.5	38.0
90-91	36.69614803933972	38.0	38.0	38.0	34.0	38.0
92-93	36.66557141195041	38.0	38.0	38.0	34.0	38.0
94-95	36.63582508515253	38.0	38.0	38.0	34.0	38.0
96-97	36.6366673347739	38.0	38.0	38.0	34.0	38.0
98-99	36.64460441925321	38.0	38.0	38.0	34.0	38.0
100	35.13835779429724	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	10.0
20	30.0
21	118.0
22	383.0
23	1199.0
24	3170.0
25	6293.0
26	11481.0
27	19119.0
28	30478.0
29	44297.0
30	59656.0
31	76525.0
32	99526.0
33	133913.0
34	200761.0
35	360467.0
36	1127195.0
37	4316712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31138480233197	18.502641520130364	15.622680562928629	23.56329311460904
2	14.54192418125535	21.40071401083741	41.814371355267994	22.242990452639244
3	18.03138575055005	26.600968753822468	31.188345622229146	24.17929987339834
4	12.388416686975782	17.827141338195606	39.5702087920857	30.214233182742912
5	13.162910327578073	37.54409337489436	35.84578402788454	13.447212269643021
6	29.289196089903747	38.18160867056173	19.29416890119665	13.23502633833788
7	25.91813891065293	32.86897092710316	24.07100046246244	17.141889699781462
8	24.005636434275953	36.88456832782394	21.789390723453316	17.320404514446793
9	26.076935140336243	16.495243353527425	21.537023645972525	35.8907978601638
10-11	24.08570652812306	26.54838082290437	29.492003766558124	19.873908882414447
12-13	24.986466580541638	24.36520020987969	29.49300282930884	21.15533038026983
14-15	22.244705174803716	25.454990943315536	27.188855363871056	25.111448518009695
16-17	21.62876128157246	28.43124175632204	27.83007909925504	22.109917862850466
18-19	21.626704694779413	27.627193345963498	29.646052940087237	21.10004901916985
20-21	22.617991806542843	26.865615194051955	29.375791543019965	21.140601456385237
22-23	22.309187793176616	26.768626673442125	29.2515360900572	21.67064944332406
24-25	21.946635627193537	26.88825943740698	29.407232854594138	21.757872080805342
26-27	21.852812898941192	27.01913839103768	29.371178157842227	21.75687055217891
28-29	21.899155965791625	27.041934276852313	29.357253865744497	21.701655891611566
30-31	22.149052727863847	26.94340037220903	29.2513679322216	21.656178967705518
32-33	21.88548892769743	27.14564158923064	29.20027008765323	21.7685993954187
34-35	22.071191795745854	27.089487252722922	29.188146268139235	21.65117468339199
36-37	22.07112970711297	26.941801834677282	29.207802951848905	21.779265506360844
38-39	21.878062742665527	27.17834336104856	29.2981283155529	21.645465580733006
40-41	22.163685438823855	27.0201035456651	29.09316840088685	21.723042614624195
42-43	22.01968625482637	27.125104921976295	28.9539349380997	21.901273885097634
44-45	22.102382846016873	27.282054464869	28.737333472392933	21.87822921672119
46-47	22.18347753606077	27.1932478006309	28.5119126185964	22.111362044711928
48-49	22.134641238331284	27.28517718261821	28.50281496336795	22.077366615682557
50-51	22.093832077955728	27.464738948534123	28.384176455192424	22.05725251831773
52-53	22.316322507031767	27.386875487658337	28.225351613207117	22.07145039210278
54-55	22.28537368426372	27.26260449399892	28.28900763349852	22.16301418823884
56-57	22.237824423638884	27.439258904425333	28.239692303905027	22.083224368030752
58-59	22.219727823886323	27.394952877146643	28.27553316913422	22.10978612983281
60-61	22.246206391601756	27.372427567589014	28.335121554682562	22.04624448612667
62-63	22.246359467308125	27.419185636089754	28.32888458986622	22.0055703067359
64-65	22.20800605986617	27.300175991642867	28.37510662485886	22.116711323632106
66-67	22.265527406744518	27.35592103684486	28.27165233740368	22.10689921900694
68-69	22.252089520143137	27.344791075612168	28.365090569860378	22.038028834384313
70-71	22.220424215032537	27.021436598139182	28.36495319583171	22.393185990996574
72-73	22.327484328668664	27.09309587910318	28.369331687735873	22.210088104492286
74-75	22.151520210654894	27.330548478769444	28.310224577941824	22.207706732633838
76-77	22.26446189851858	27.294783123041565	28.142578076410523	22.298176902029322
78-79	22.22244222855319	27.316096577607585	28.04571472971894	22.41574646412029
80-81	22.245014088170066	27.38167906688238	28.022013329302492	22.35129351564506
82-83	22.380667096408803	27.27234317428745	28.064603821184125	22.282385908119622
84-85	22.413841786392776	27.29480718571102	27.982293340557636	22.30905768733857
86-87	22.23751302692247	27.37912535551366	28.04455477091734	22.33880684664653
88-89	22.348680991676545	27.35495915106793	27.96036902589635	22.33599083135917
90-91	22.402573559656126	27.44355139354331	27.925361472930714	22.228513573869854
92-93	22.46676455987101	27.39198551768165	27.879996104850996	22.261253817596348
94-95	22.29535317475822	27.421142571598374	27.927240797139152	22.356263456504262
96-97	22.400426633555643	27.54002644182573	27.967925506886203	22.091621417732423
98-99	23.520648620705497	29.44993670552304	28.177030793051618	18.852383880719845
100	29.873462086039886	40.5529378145187	29.57360009944141	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1470.0
1	1338.0
2	1767.5
3	2543.0
4	3245.0
5	4697.5
6	6064.0
7	7593.5
8	9116.5
9	9581.0
10	9148.5
11	8686.0
12	9524.0
13	11988.5
14	16809.0
15	22594.5
16	26664.0
17	28468.0
18	28070.0
19	25888.5
20	23294.0
21	21697.0
22	22511.5
23	26120.5
24	32447.5
25	41990.0
26	54823.5
27	68635.0
28	81571.5
29	95847.5
30	109343.0
31	122173.5
32	138117.0
33	155668.0
34	172217.0
35	188091.5
36	204435.5
37	217308.5
38	227548.5
39	236150.0
40	244900.5
41	253307.0
42	258088.0
43	262470.5
44	267248.5
45	271396.0
46	273752.0
47	275215.0
48	275203.0
49	272389.0
50	267595.5
51	260525.0
52	248918.0
53	234837.0
54	218621.0
55	201299.0
56	183302.5
57	163886.0
58	145099.0
59	124294.5
60	103292.5
61	83536.0
62	65642.0
63	50547.0
64	37464.0
65	27378.5
66	19976.5
67	14863.5
68	11241.0
69	8206.0
70	5786.5
71	4071.0
72	2806.5
73	1818.0
74	1143.0
75	732.0
76	479.5
77	298.5
78	185.5
79	107.0
80	65.0
81	43.0
82	28.0
83	18.5
84	12.0
85	9.5
86	8.0
87	4.5
88	2.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018486173420641477
3	0.0017253761859265379
4	0.0
5	0.0
6	6.162057806880492E-4
7	0.0
8	0.0
9	0.0
10-11	7.702572258600616E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4635459963235727E-4
24-25	8.627707636568909E-4
26-27	0.0027966030594837473
28-29	0.001718635745403921
30-31	0.002484212137521046
32-33	0.003316837286632264
34-35	0.0010854039222156133
36-37	0.0
38-39	3.91498469789081E-5
40-41	2.364343617623849E-5
42-43	6.188761510600515E-4
44-45	0.0
46-47	0.0
48-49	2.2815276359405463E-4
50-51	0.005712661040531207
52-53	0.0
54-55	0.0029651398117712543
56-57	0.04647306512995732
58-59	0.05732504337226389
60-61	0.036423849302955
62-63	0.00815096170087443
64-65	0.0
66-67	0.0
68-69	6.32731578866694E-4
70-71	0.0023952844233663417
72-73	0.010144435840628456
74-75	0.0047685060298172195
76-77	0.0038429171416939243
78-79	0.0038004388615424114
80-81	0.015962620515649582
82-83	0.05300628051964006
84-85	0.03414335533930784
86-87	0.00914619531508937
88-89	0.00809923536080557
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	34.0
20-21	154.0
22-23	334.0
24-25	556.0
26-27	1632.0
28-29	4528.0
30-31	13427.0
32-33	16467.0
34-35	22636.0
36-37	35342.0
38-39	41862.0
40-41	41378.0
42-43	47637.0
44-45	56959.0
46-47	58304.0
48-49	54487.0
50-51	49158.0
52-53	47748.0
54-55	51509.0
56-57	54729.0
58-59	54346.0
60-61	53123.0
62-63	50487.0
64-65	52778.0
66-67	56792.0
68-69	57670.0
70-71	57965.0
72-73	53890.0
74-75	54782.0
76-77	57424.0
78-79	59623.0
80-81	59548.0
82-83	55949.0
84-85	54014.0
86-87	55791.0
88-89	59293.0
90-91	59974.0
92-93	60244.0
94-95	68548.0
96-97	200205.0
98-99	1488575.0
100-101	3121436.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.17380412055765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.86388027769434	78.30086220683835
2	6.407626235874354	11.043390562559656
3	1.481479731262234	3.8299423251110443
4	0.55490723688167	1.9127387014448365
5	0.24610415961841264	1.0603865822105774
6	0.14385531869624915	0.7437936033018582
7	0.08699096986055012	0.5247439957914266
8	0.05497127691348587	0.3789667239199732
9	0.03762495530920085	0.29180569759738284
>10	0.11958715582799335	1.6363694549103076
>50	0.0022623613386301747	0.12151339938829106
>100	6.243604372974567E-4	0.09696730107646123
>500	8.59602856007565E-5	0.058519445849870975
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.081028903440246E-5	0.0	1.540514451720123E-5	1.540514451720123E-5
3	0.0	3.081028903440246E-5	0.0	1.540514451720123E-5	1.540514451720123E-5
4	0.0	7.702572258600616E-5	0.0	1.540514451720123E-5	1.540514451720123E-5
5	1.540514451720123E-5	7.702572258600616E-5	0.0	1.540514451720123E-5	1.540514451720123E-5
6	6.162057806880492E-5	9.243086710320739E-5	0.0	1.540514451720123E-5	1.540514451720123E-5
7	6.162057806880492E-5	9.243086710320739E-5	0.0	1.540514451720123E-5	3.081028903440246E-5
8	7.702572258600616E-5	1.2324115613760985E-4	0.0	1.540514451720123E-5	3.081028903440246E-5
9	7.702572258600616E-5	2.3107716775801847E-4	0.0	3.081028903440246E-5	3.081028903440246E-5
10-11	1.0783601162040862E-4	2.3107716775801847E-4	0.0	4.621543355160369E-5	3.081028903440246E-5
12-13	1.0783601162040862E-4	2.3107716775801847E-4	0.0	4.621543355160369E-5	3.851286129300308E-5
14-15	1.0783601162040862E-4	3.4661575163702767E-4	0.0	4.621543355160369E-5	5.391800581020431E-5
16-17	1.6175401743061293E-4	3.6972346841282954E-4	0.0	6.162057806880492E-5	7.702572258600616E-5
18-19	1.6945658968921354E-4	4.236414742230339E-4	0.0	6.162057806880492E-5	7.702572258600616E-5
20-21	1.8486173420641477E-4	5.0066719680904E-4	0.0	8.472829484460677E-5	7.702572258600616E-5
22-23	2.00266878723616E-4	6.316109252052504E-4	0.0	9.243086710320739E-5	1.00133439361808E-4
24-25	2.00266878723616E-4	7.317443645670585E-4	0.0	9.243086710320739E-5	1.2324115613760985E-4
26-27	2.00266878723616E-4	7.702572258600616E-4	0.0	1.3864630065481108E-4	1.3864630065481108E-4
28-29	2.00266878723616E-4	7.702572258600616E-4	0.0	3.4661575163702767E-4	1.3864630065481108E-4
30-31	2.00266878723616E-4	9.089035265148726E-4	0.0	0.001555919596237324	1.3864630065481108E-4
32-33	2.00266878723616E-4	9.089035265148726E-4	0.0	0.0036125063892836883	1.3864630065481108E-4
34-35	2.0796945098221662E-4	0.0010321446826524825	0.0	0.005992601217191279	1.540514451720123E-4
36-37	2.1567202324081723E-4	0.001163088411048693	0.0	0.009728348762612577	1.540514451720123E-4
38-39	2.1567202324081723E-4	0.0011861961278244947	0.0	0.015582303679149045	1.6175401743061293E-4
40-41	2.3107716775801847E-4	0.001278626994927702	0.0	0.021952330937011752	1.7715916194781416E-4
42-43	2.3107716775801847E-4	0.0012940321394449032	0.0	0.027390346951583787	1.8486173420641477E-4
44-45	2.3877974001661908E-4	0.0014326784400997144	0.0	0.0337988870707395	1.8486173420641477E-4
46-47	2.464823122752197E-4	0.001540514451720123	0.0	0.04113173586092728	1.925643064650154E-4
48-49	2.464823122752197E-4	0.001540514451720123	0.0	0.04870336439113169	2.00266878723616E-4
50-51	2.7729260130962216E-4	0.001640647891081931	0.0	0.055774325724527056	2.00266878723616E-4
52-53	2.7729260130962216E-4	0.0016483504633405316	0.0	0.06194408610366614	2.2337459549941785E-4
54-55	2.7729260130962216E-4	0.001663755607857733	0.0	0.0688378882751137	2.541848845338203E-4
56-57	2.7729260130962216E-4	0.0017099710414093365	0.0	0.07569317758526825	2.6959002905102157E-4
58-59	2.7729260130962216E-4	0.001717673613667937	0.0	0.08293359550835283	2.7729260130962216E-4
60-61	3.081028903440246E-4	0.0017330787581851385	0.0	0.09018941857595461	2.7729260130962216E-4
62-63	3.543183238956283E-4	0.0017484839027023396	0.0	0.09743753907129778	2.926977458268234E-4
64-65	3.543183238956283E-4	0.0019102379201329526	0.0	0.10489362901762318	2.926977458268234E-4
66-67	3.6972346841282954E-4	0.0019333456369087544	0.0	0.11204161607360455	2.926977458268234E-4
68-69	3.6972346841282954E-4	0.00200266878723616	0.0	0.11821907902500224	3.0040031808542403E-4
70-71	3.6972346841282954E-4	0.002018073931753361	0.0	0.12387276706281508	3.389131793784271E-4
72-73	4.7755948003323816E-4	0.002018073931753361	0.0	0.1278704020650288	3.543183238956283E-4
74-75	4.7755948003323816E-4	0.002048884220787764	0.0	0.13092062067943466	3.543183238956283E-4
76-77	4.7755948003323816E-4	0.0021105047988565686	0.0	0.1339785418660991	3.543183238956283E-4
78-79	4.929646245504394E-4	0.002187530521442575	0.0	0.1362430981101277	3.543183238956283E-4
80-81	4.929646245504394E-4	0.0021952330937011757	0.0	0.13704416562502214	3.6202089615422896E-4
82-83	5.0066719680904E-4	0.0022106382382183765	0.0	0.13774509970055482	3.6972346841282954E-4
84-85	5.160723413262412E-4	0.0022491510995113796	0.0	0.13807631030767464	3.6972346841282954E-4
86-87	5.237749135848419E-4	0.002295366533062983	0.0	0.13823806432510524	3.6972346841282954E-4
88	5.237749135848419E-4	0.002464823122752197	0.0	0.13824576689736384	3.6972346841282954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7860	0.0	52.000404	1
GTATCAA	15015	0.0	39.108994	1
TCAACGC	18445	0.0	31.054813	4
ATCAACG	18695	0.0	30.684887	3
CAACGCA	19000	0.0	30.147684	5
AACGCAG	19420	0.0	29.53934	6
TATCAAC	19650	0.0	29.495665	2
ACGCAGA	22360	0.0	25.674328	7
CGCAGAG	23205	0.0	24.684593	8
GTACATG	21025	0.0	23.04955	1
TACATGG	21140	0.0	22.643377	2
ACATGGG	20945	0.0	22.287388	3
GCAGAGT	26740	0.0	21.389605	9
TGGTATC	3845	0.0	20.179338	2
GTGGTAT	4065	0.0	20.025938	1
CATGGGG	14110	0.0	19.651829	4
GAGTACT	16505	0.0	19.22775	12-13
AGAGTAC	24520	0.0	19.011965	10-11
CAGAGTA	26235	0.0	18.625677	10-11
GTACTTT	18440	0.0	17.198792	14-15
>>END_MODULE