FastQCFastQC Report
Fri 27 May 2016
SRR2139162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6804817
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87900.054.47011
GTATCAA169550.038.4759451
TCAACGC206600.031.1449834
ATCAACG210300.030.5974713
CAACGCA211300.030.4722545
TATCAAC218100.029.65872
AACGCAG218450.029.5330146
ACGCAGA248850.025.8399567
CGCAGAG253600.025.305898
GCAGAGT296800.021.665349
GTACATG226900.021.2134061
TACATGG222100.020.9475672
ACATGGG219600.020.6075613
GAGTACT179200.019.86687912-13
AGAGTAC274050.019.20824210-11
CAGAGTA291500.019.1402910-11
CATGGGG141100.017.8510914
GTACTTT205200.017.33944714-15
AGTACTT196150.017.17894412-13
GTGGTAT41100.016.2742021
Produced by FastQC (version 0.11.5)