FastQCFastQC Report
Fri 27 May 2016
SRR2139161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531452
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91350.052.4137841
GTATCAA166700.036.6936231
TCAACGC201800.029.7668294
ATCAACG204850.029.2826423
CAACGCA206750.029.0133485
TATCAAC214600.028.2085252
AACGCAG213350.028.1751336
ACGCAGA246800.024.3220757
CGCAGAG255650.023.4966038
GTACATG246850.022.455381
TACATGG248350.021.8611282
ACATGGG242400.021.7706473
GCAGAGT298600.020.0886469
CATGGGG156850.019.0142274
GAGTACT175100.018.8030212-13
GTGGTAT43950.018.3329641
CAGAGTA295750.018.19980610-11
AGAGTAC274850.017.98758110-11
TGGTATC42950.017.1883052
GTACTTT201550.016.30432114-15
Produced by FastQC (version 0.11.5)