##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7531452
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.694538450221817	18.0	18.0	31.0	18.0	33.0
2	30.68045657065862	31.0	29.0	33.0	27.0	33.0
3	31.775425774472172	33.0	31.0	33.0	29.0	33.0
4	32.60724452602234	33.0	33.0	33.0	31.0	34.0
5	33.00250164244557	33.0	33.0	34.0	32.0	34.0
6	36.990392025335886	38.0	38.0	38.0	35.0	38.0
7	37.10072267605237	38.0	38.0	38.0	36.0	38.0
8	37.082477323097855	38.0	38.0	38.0	36.0	38.0
9	37.28809955902262	38.0	38.0	38.0	36.0	38.0
10-11	37.31263878465931	38.0	38.0	38.0	37.0	38.0
12-13	37.367661508033244	38.0	38.0	38.0	37.0	38.0
14-15	37.27288781764791	38.0	38.0	38.0	36.5	38.0
16-17	37.345347816065214	38.0	38.0	38.0	37.0	38.0
18-19	37.37877742565444	38.0	38.0	38.0	37.0	38.0
20-21	37.39584057722546	38.0	38.0	38.0	37.0	38.0
22-23	37.41394816978537	38.0	38.0	38.0	37.0	38.0
24-25	37.425752798597	38.0	38.0	38.0	37.0	38.0
26-27	37.39344836685184	38.0	38.0	38.0	37.0	38.0
28-29	37.37099279719098	38.0	38.0	38.0	37.0	38.0
30-31	37.3702046751003	38.0	38.0	38.0	37.0	38.0
32-33	37.35591818868918	38.0	38.0	38.0	37.0	38.0
34-35	37.32880533059165	38.0	38.0	38.0	37.0	38.0
36-37	37.30570565512407	38.0	38.0	38.0	37.0	38.0
38-39	37.3037653636991	38.0	38.0	38.0	37.0	38.0
40-41	37.29431862692671	38.0	38.0	38.0	37.0	38.0
42-43	37.25780647201844	38.0	38.0	38.0	37.0	38.0
44-45	37.17095678078374	38.0	38.0	38.0	37.0	38.0
46-47	37.18852845213799	38.0	38.0	38.0	37.0	38.0
48-49	37.143580545176604	38.0	38.0	38.0	36.5	38.0
50-51	37.09399734116616	38.0	38.0	38.0	36.0	38.0
52-53	37.069515419690376	38.0	38.0	38.0	36.0	38.0
54-55	37.04292042475187	38.0	38.0	38.0	36.0	38.0
56-57	36.99789125100439	38.0	38.0	38.0	36.0	38.0
58-59	36.98613967270467	38.0	38.0	38.0	36.0	38.0
60-61	36.99451556557125	38.0	38.0	38.0	36.0	38.0
62-63	36.998696936032445	38.0	38.0	38.0	36.0	38.0
64-65	36.959424525388116	38.0	38.0	38.0	36.0	38.0
66-67	36.98551742560625	38.0	38.0	38.0	36.0	38.0
68-69	36.971806737668594	38.0	38.0	38.0	36.0	38.0
70-71	36.977678983514906	38.0	38.0	38.0	36.0	38.0
72-73	36.96138225589701	38.0	38.0	38.0	36.0	38.0
74-75	36.954266000597656	38.0	38.0	38.0	36.0	38.0
76-77	36.94058504810045	38.0	38.0	38.0	36.0	38.0
78-79	36.91932455442587	38.0	38.0	38.0	35.5	38.0
80-81	36.88511237849073	38.0	38.0	38.0	35.0	38.0
82-83	36.849695140291004	38.0	38.0	38.0	35.0	38.0
84-85	36.828185158399684	38.0	38.0	38.0	35.0	38.0
86-87	36.819613640801144	38.0	38.0	38.0	35.0	38.0
88-89	36.802283204995696	38.0	38.0	38.0	35.0	38.0
90-91	36.777353368847116	38.0	38.0	38.0	35.0	38.0
92-93	36.750975482834676	38.0	38.0	38.0	35.0	38.0
94-95	36.72195171843561	38.0	38.0	38.0	34.5	38.0
96-97	36.72238362284263	38.0	38.0	38.0	34.0	38.0
98-99	36.72873961804649	38.0	38.0	38.0	34.0	38.0
100	35.286817082565435	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	1.0
19	13.0
20	28.0
21	102.0
22	457.0
23	1469.0
24	3346.0
25	7295.0
26	13049.0
27	21746.0
28	33613.0
29	49320.0
30	66141.0
31	85785.0
32	111433.0
33	150099.0
34	221959.0
35	391858.0
36	1206944.0
37	5166790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21863194507513	19.009986387750992	15.159161872106466	23.612219795067404
2	14.446398219481418	21.397240233378376	42.132800905661696	22.023560641478504
3	18.07030306957345	26.74132126070548	31.076916662672225	24.111459007048843
4	12.267953111830229	17.96066681431416	39.70851835741634	30.062861716439272
5	13.096863659225338	37.656204938967946	35.92097513201969	13.32595626978702
6	29.089740653329216	38.327620603006736	19.44160633611833	13.141032407545715
7	25.799221717140338	32.811627824223	24.242324056503314	17.146826402133346
8	23.799726799028925	37.17018975889377	21.868253292990513	17.16183014908679
9	25.897914505728775	16.58159674920586	21.784046422920838	35.736442322144526
10-11	23.93341947874062	26.780785431547592	29.555847929456363	19.72994716025542
12-13	24.84089389403265	24.52990472487908	29.550304509674895	21.078896871413374
14-15	22.109328984636694	25.660609667299216	27.242402925757208	24.98765842230688
16-17	21.48765603232949	28.62392271769109	27.93679757900601	21.951623670973405
18-19	21.494002750067317	27.86151329119538	29.653398839958083	20.99108511877922
20-21	22.45021510346948	27.06696140698336	29.451293458943855	21.031530030603307
22-23	22.138102698539093	26.993098928198073	29.2726249341236	21.596173439139232
24-25	21.797318121196255	27.141409440761016	29.396472696559467	21.66479974148326
26-27	21.728808467648488	27.25065842238133	29.382152719462727	21.638380390507457
28-29	21.748430964184408	27.222797140672483	29.394926064025924	21.633845831117192
30-31	21.95400598158311	27.169381210381154	29.293445029450737	21.583167778585004
32-33	21.75131433720614	27.332262046313726	29.24864167382043	21.667781942659705
34-35	21.91762317158523	27.266972301745763	29.267175630685667	21.548228895983346
36-37	21.902562611297636	27.181433674663985	29.210856795788036	21.705146918250342
38-39	21.725387673486207	27.408324635801407	29.28971394408585	21.57657374662654
40-41	21.988742379877817	27.218630236274798	29.146195339584153	21.646432044263232
42-43	21.838895289657557	27.31880259529423	28.986173723231584	21.856128391816632
44-45	21.94213387075597	27.42139423822392	28.838455288871	21.798016602149104
46-47	22.02124168124116	27.420817578491295	28.56964422429548	21.98829651597206
48-49	21.938435679406982	27.48746324706038	28.57514957315362	21.998951500379015
50-51	21.979864305119676	27.62604744154095	28.43811023839171	21.955978014947657
52-53	22.14222538049938	27.55864522713725	28.32863772113295	21.97049167123042
54-55	22.074934285604844	27.480535023947724	28.385532642546213	22.058998047901223
56-57	22.055624840041386	27.635579773217998	28.332300573249352	21.976494813491264
58-59	22.056259735247696	27.586031525894978	28.353448944939245	22.004259793918077
60-61	22.103394582935152	27.59363686029005	28.39061819636125	21.912350360413548
62-63	22.068839689234558	27.575498047350326	28.47359220838999	21.88207005502512
64-65	22.051841742938603	27.4806861619875	28.44280160143583	22.02467049363807
66-67	22.080512687317338	27.501536028207784	28.382386182300667	22.035565102174207
68-69	22.059574713113385	27.549334467629826	28.432624521855637	21.95846629740115
70-71	22.046139956888375	27.266036792009263	28.425679089484486	22.26214416161788
72-73	22.13628178560767	27.301400751666073	28.43674867118126	22.125568791544996
74-75	22.031477021774105	27.53071058127567	28.343469267434774	22.094343129515455
76-77	22.142150406171027	27.438339099764512	28.222370133296103	22.197140360768362
78-79	22.073397311329035	27.451548255946413	28.193569011803753	22.2814854209208
80-81	22.11639695323125	27.55317807289106	28.095620673072748	22.234804300804946
82-83	22.202068238945202	27.520060960242677	28.133463358191896	22.144407442620224
84-85	22.22129890843437	27.498037206010356	28.080909592329505	22.19975429322577
86-87	22.109084519621053	27.554242679530883	28.13990416168507	22.196768639162993
88-89	22.250440666758127	27.533776517362895	28.041656586761693	22.174126229117284
90-91	22.28139519170596	27.579418825236125	28.047125793018328	22.092060190039586
92-93	22.261381548117747	27.579974368441235	28.02618359580239	22.13246048763863
94-95	22.15379522738568	27.610578607024934	28.028761334042006	22.206864831547385
96-97	22.303179673551675	27.689570655748035	28.069746131626026	21.93750353907426
98-99	23.32659778440457	29.60740396399442	28.3231927313474	18.742805520253608
100	29.685474054207152	40.579109403656524	29.735416542136324	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1385.0
1	1347.0
2	1942.5
3	2808.0
4	3636.0
5	5287.5
6	6791.5
7	8420.0
8	9970.5
9	10329.0
10	9767.0
11	9263.5
12	10240.0
13	12891.5
14	18051.0
15	24282.0
16	28775.5
17	31072.0
18	31039.0
19	29110.5
20	26892.0
21	25631.5
22	26521.5
23	31090.5
24	39203.5
25	50140.5
26	64504.0
27	81029.0
28	97413.5
29	115049.5
30	133144.0
31	150533.5
32	169615.0
33	190584.0
34	209580.0
35	227055.5
36	246709.5
37	263518.5
38	276706.5
39	287175.0
40	296878.5
41	305384.0
42	309125.0
43	313321.5
44	318607.5
45	322185.5
46	321998.5
47	320397.0
48	317721.0
49	312185.5
50	305278.5
51	296195.0
52	283272.5
53	267211.5
54	247655.5
55	226290.5
56	204188.0
57	181471.0
58	159331.0
59	135507.5
60	112067.5
61	90682.0
62	71608.0
63	54875.0
64	40482.0
65	29967.5
66	22351.0
67	16907.5
68	12834.0
69	9394.5
70	6904.0
71	4834.5
72	3279.0
73	2260.0
74	1511.0
75	992.0
76	643.0
77	392.5
78	237.5
79	159.5
80	113.5
81	79.0
82	53.5
83	38.0
84	22.0
85	11.0
86	5.5
87	3.0
88	1.5
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0021642573039036828
3	0.0019252595648222945
4	0.0
5	0.0
6	3.5849660862208245E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.8589426360842278E-4
24-25	0.0011486255002828409
26-27	0.002443583435425984
28-29	0.0014547118315499496
30-31	0.002619991103999749
32-33	0.0035189940369046505
34-35	9.691785189401545E-4
36-37	0.0
38-39	4.728752986629721E-5
40-41	6.801858131604391E-6
42-43	5.68497356007839E-4
44-45	0.0
46-47	0.0
48-49	2.74623055211064E-4
50-51	0.0064100126956626174
52-53	0.0
54-55	0.0026379017334627955
56-57	0.04657473972853747
58-59	0.05817216958546528
60-61	0.03798454566101701
62-63	0.008742443119291688
64-65	0.0
66-67	0.0
68-69	6.346323845475993E-4
70-71	0.0029268217861344713
72-73	0.009857514610442653
74-75	0.004676367397805385
76-77	0.003635700837083761
78-79	0.003922336431216437
80-81	0.016429441625540695
82-83	0.05389871859414608
84-85	0.0350717388781307
86-87	0.009101210940066131
88-89	0.008282507413579781
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	43.0
20-21	164.0
22-23	402.0
24-25	621.0
26-27	1928.0
28-29	5253.0
30-31	16387.0
32-33	19954.0
34-35	27854.0
36-37	44473.0
38-39	51142.0
40-41	49962.0
42-43	57259.0
44-45	68836.0
46-47	69702.0
48-49	66273.0
50-51	59889.0
52-53	57812.0
54-55	62392.0
56-57	66597.0
58-59	66852.0
60-61	65158.0
62-63	61663.0
64-65	64109.0
66-67	68665.0
68-69	71097.0
70-71	70385.0
72-73	65625.0
74-75	65355.0
76-77	68819.0
78-79	71858.0
80-81	71019.0
82-83	66954.0
84-85	65148.0
86-87	67075.0
88-89	71047.0
90-91	72211.0
92-93	71196.0
94-95	80436.0
96-97	229591.0
98-99	1690128.0
100-101	3580118.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.82137933101332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.9103296013231	78.92960211423922
2	6.374778967792789	11.069342058282066
3	1.5444274096174808	4.022679539388408
4	0.5686326497989627	1.9747788395277994
5	0.23342783717768037	1.01332633990107
6	0.1291080530680896	0.6725603550067922
7	0.07445351959171331	0.4524910086900822
8	0.04401597077813081	0.30572218364407144
9	0.03015070756578399	0.23559534168006466
>10	0.08882046657931103	1.1500185095626665
>50	0.0014718106735024676	0.08529635651415332
>100	3.1409555354257204E-4	0.050835329532871266
>500	6.89104798381582E-5	0.03775202403077627
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.983295651356472E-5	0.0	0.0	0.0
3	0.0	3.983295651356472E-5	0.0	0.0	0.0
4	0.0	1.1949886954069414E-4	0.0	0.0	0.0
5	0.0	1.1949886954069414E-4	0.0	0.0	0.0
6	1.3277652171188239E-5	1.1949886954069414E-4	0.0	0.0	0.0
7	2.6555304342376478E-5	1.3277652171188239E-4	0.0	0.0	0.0
8	5.3110608684752955E-5	1.4605417388307063E-4	0.0	0.0	0.0
9	5.3110608684752955E-5	2.6555304342376477E-4	0.0	1.3277652171188239E-5	0.0
10-11	5.974943477034707E-5	2.6555304342376477E-4	0.0	3.3194130427970596E-5	0.0
12-13	6.638826085594119E-5	2.721918695093589E-4	0.0	4.647178259915884E-5	0.0
14-15	6.638826085594119E-5	3.3858013036530004E-4	0.0	6.638826085594119E-5	3.3194130427970596E-5
16-17	7.966591302712944E-5	3.452189564508942E-4	0.0	6.638826085594119E-5	3.983295651356472E-5
18-19	7.966591302712944E-5	3.6513543470767653E-4	0.0	9.294356519831767E-5	3.983295651356472E-5
20-21	7.966591302712944E-5	3.850519129644589E-4	0.0	1.0622121736950591E-4	5.3110608684752955E-5
22-23	7.966591302712944E-5	4.44801347734806E-4	0.0	1.1286004345510003E-4	5.974943477034707E-5
24-25	7.966591302712944E-5	4.514401738204001E-4	0.0	1.3277652171188239E-4	6.638826085594119E-5
26-27	7.966591302712944E-5	4.514401738204001E-4	0.0	1.9916478256782358E-4	6.638826085594119E-5
28-29	9.294356519831767E-5	4.514401738204001E-4	0.0	4.3816252164921186E-4	7.966591302712944E-5
30-31	9.294356519831767E-5	5.311060868475295E-4	0.0	0.0015402076518578358	7.966591302712944E-5
32-33	9.294356519831767E-5	5.311060868475295E-4	0.0	0.004149266303496324	9.294356519831767E-5
34-35	1.1949886954069414E-4	5.974943477034707E-4	0.0	0.007362458128923878	9.294356519831767E-5
36-37	1.1949886954069414E-4	6.572437824738178E-4	0.0	0.011783916301929563	9.294356519831767E-5
38-39	1.1949886954069414E-4	7.169932172441649E-4	0.0	0.018801155474402545	9.294356519831767E-5
40-41	1.4605417388307063E-4	7.701038259289178E-4	0.0	0.02620344655983999	1.3277652171188239E-4
42-43	1.5933182605425887E-4	7.701038259289178E-4	0.0	0.033778347123502875	1.3277652171188239E-4
44-45	1.6597065213985298E-4	8.298532606992649E-4	0.0	0.04240218220868964	1.4605417388307063E-4
46-47	1.9916478256782358E-4	8.763250432984237E-4	0.0	0.052499836684878294	1.4605417388307063E-4
48-49	2.058036086534177E-4	8.89602695469612E-4	0.0	0.0619734415090211	1.5933182605425887E-4
50-51	2.1244243473901182E-4	9.759074345823355E-4	0.0	0.07122132624625371	1.5933182605425887E-4
52-53	2.1244243473901182E-4	9.825462606679297E-4	0.0	0.08022357441831934	1.7924830431104123E-4
54-55	2.1244243473901182E-4	9.891850867535237E-4	0.0	0.08935859911209684	1.9916478256782358E-4
56-57	2.1244243473901182E-4	9.95823912839118E-4	0.0	0.09849362380587434	1.9916478256782358E-4
58-59	2.2572008691020004E-4	0.0010024627389247122	0.0	0.10738301193448488	1.9916478256782358E-4
60-61	2.3235891299579415E-4	0.0010356568693526825	0.0	0.11572801632407669	1.9916478256782358E-4
62-63	2.3899773908138828E-4	0.0010754898258662474	0.0	0.1238539394528439	2.1244243473901182E-4
64-65	2.3899773908138828E-4	0.0011551557388933768	0.0	0.13112345401656944	2.1908126082460593E-4
66-67	2.3899773908138828E-4	0.0011551557388933768	0.0	0.13845271801506537	2.2572008691020004E-4
68-69	2.3899773908138828E-4	0.001168433391064565	0.0	0.14571559375270532	2.3235891299579415E-4
70-71	2.3899773908138828E-4	0.001175072217150159	0.0	0.15177020314276718	2.3899773908138828E-4
72-73	2.5227539125257655E-4	0.0011817110432357533	0.0	0.15646385318528222	2.5227539125257655E-4
74-75	2.5891421733817064E-4	0.0012149051736637237	0.0	0.15998906983673267	2.5227539125257655E-4
76-77	2.6555304342376477E-4	0.001274654608434071	0.0	0.16377320070552132	2.5227539125257655E-4
78-79	2.6555304342376477E-4	0.0013144875649476356	0.0	0.16630259344413267	2.5227539125257655E-4
80-81	2.78830695594953E-4	0.0013211263910332298	0.0	0.16723202909611587	2.5891421733817064E-4
82-83	2.9210834776614126E-4	0.0013277652171188239	0.0	0.16800213292204477	2.6555304342376477E-4
84-85	2.9210834776614126E-4	0.0013410428692900121	0.0	0.16836062953066688	2.6555304342376477E-4
86-87	2.9874717385173534E-4	0.0013543205214612004	0.0	0.16847348957412195	2.6555304342376477E-4
88	3.1866365210851775E-4	0.0014339864344883298	0.0	0.16848012840020757	2.6555304342376477E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9135	0.0	52.413784	1
GTATCAA	16670	0.0	36.693623	1
TCAACGC	20180	0.0	29.766829	4
ATCAACG	20485	0.0	29.282642	3
CAACGCA	20675	0.0	29.013348	5
TATCAAC	21460	0.0	28.208525	2
AACGCAG	21335	0.0	28.175133	6
ACGCAGA	24680	0.0	24.322075	7
CGCAGAG	25565	0.0	23.496603	8
GTACATG	24685	0.0	22.45538	1
TACATGG	24835	0.0	21.861128	2
ACATGGG	24240	0.0	21.770647	3
GCAGAGT	29860	0.0	20.088646	9
CATGGGG	15685	0.0	19.014227	4
GAGTACT	17510	0.0	18.80302	12-13
GTGGTAT	4395	0.0	18.332964	1
CAGAGTA	29575	0.0	18.199806	10-11
AGAGTAC	27485	0.0	17.987581	10-11
TGGTATC	4295	0.0	17.188305	2
GTACTTT	20155	0.0	16.304321	14-15
>>END_MODULE