FastQCFastQC Report
Fri 27 May 2016
SRR2139160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6599691
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.050.6478581
GTATCAA140900.037.7494471
TCAACGC174800.029.9489044
ATCAACG176700.029.650763
CAACGCA176700.029.5322195
AACGCAG181500.028.9355036
TATCAAC184750.028.7444322
ACGCAGA209850.024.9863787
CGCAGAG215550.024.2286438
GTACATG197900.021.1294751
TACATGG193800.021.1018032
ACATGGG192400.020.6896513
GCAGAGT255150.020.550239
GAGTACT149550.018.99843412-13
AGAGTAC227150.018.94163110-11
GTGGTAT39250.018.217591
CAGAGTA247100.018.13997810-11
CATGGGG139850.017.9094544
AGTACTT163950.017.21500212-13
GTACTTT172350.016.5459914-15
Produced by FastQC (version 0.11.5)