##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6599691
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.825522740382844	18.0	18.0	30.0	18.0	33.0
2	30.3627422859646	31.0	29.0	33.0	27.0	33.0
3	31.587412350063055	33.0	31.0	33.0	29.0	33.0
4	32.541309737077086	33.0	33.0	33.0	31.0	34.0
5	32.96333388941997	33.0	33.0	34.0	32.0	34.0
6	36.95949946747507	38.0	38.0	38.0	35.0	38.0
7	37.0597726469315	38.0	38.0	38.0	36.0	38.0
8	37.047865422790245	38.0	38.0	38.0	36.0	38.0
9	37.26995172955825	38.0	38.0	38.0	36.0	38.0
10-11	37.301217814591624	38.0	38.0	38.0	37.0	38.0
12-13	37.35836609623087	38.0	38.0	38.0	37.0	38.0
14-15	37.26418850518911	38.0	38.0	38.0	36.5	38.0
16-17	37.33533069957366	38.0	38.0	38.0	37.0	38.0
18-19	37.378788037197495	38.0	38.0	38.0	37.0	38.0
20-21	37.39818651811216	38.0	38.0	38.0	37.0	38.0
22-23	37.4177495610309	38.0	38.0	38.0	37.0	38.0
24-25	37.43038484972153	38.0	38.0	38.0	37.0	38.0
26-27	37.397887450388026	38.0	38.0	38.0	37.0	38.0
28-29	37.37440878858929	38.0	38.0	38.0	37.0	38.0
30-31	37.37348487085254	38.0	38.0	38.0	37.0	38.0
32-33	37.35511819235316	38.0	38.0	38.0	37.0	38.0
34-35	37.3307618761623	38.0	38.0	38.0	37.0	38.0
36-37	37.308037146325375	38.0	38.0	38.0	37.0	38.0
38-39	37.303181761703584	38.0	38.0	38.0	37.0	38.0
40-41	37.29084138473295	38.0	38.0	38.0	37.0	38.0
42-43	37.25469443252858	38.0	38.0	38.0	37.0	38.0
44-45	37.16827512787876	38.0	38.0	38.0	37.0	38.0
46-47	37.18791548938201	38.0	38.0	38.0	37.0	38.0
48-49	37.143240286373924	38.0	38.0	38.0	36.5	38.0
50-51	37.0867946799078	38.0	38.0	38.0	36.0	38.0
52-53	37.05993585913374	38.0	38.0	38.0	36.0	38.0
54-55	37.02838703640104	38.0	38.0	38.0	36.0	38.0
56-57	36.98095454475052	38.0	38.0	38.0	36.0	38.0
58-59	36.96782943881479	38.0	38.0	38.0	36.0	38.0
60-61	36.97420860931171	38.0	38.0	38.0	36.0	38.0
62-63	36.97675460585819	38.0	38.0	38.0	36.0	38.0
64-65	36.935714646760175	38.0	38.0	38.0	36.0	38.0
66-67	36.96119271238092	38.0	38.0	38.0	36.0	38.0
68-69	36.94610858361766	38.0	38.0	38.0	36.0	38.0
70-71	36.94998253951684	38.0	38.0	38.0	36.0	38.0
72-73	36.934259984331696	38.0	38.0	38.0	36.0	38.0
74-75	36.9251385758763	38.0	38.0	38.0	35.5	38.0
76-77	36.90824667690572	38.0	38.0	38.0	35.0	38.0
78-79	36.88575497412017	38.0	38.0	38.0	35.0	38.0
80-81	36.852819831264384	38.0	38.0	38.0	35.0	38.0
82-83	36.8155510611343	38.0	38.0	38.0	35.0	38.0
84-85	36.791879182055325	38.0	38.0	38.0	35.0	38.0
86-87	36.78188591240202	38.0	38.0	38.0	35.0	38.0
88-89	36.763151970498456	38.0	38.0	38.0	35.0	38.0
90-91	36.73874532097868	38.0	38.0	38.0	35.0	38.0
92-93	36.709274529542725	38.0	38.0	38.0	34.0	38.0
94-95	36.67812926521309	38.0	38.0	38.0	34.0	38.0
96-97	36.68236334101808	38.0	38.0	38.0	34.0	38.0
98-99	36.689389662593186	38.0	38.0	38.0	34.0	38.0
100	35.21092446956379	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	9.0
20	18.0
21	106.0
22	365.0
23	1212.0
24	2888.0
25	6272.0
26	11012.0
27	19085.0
28	29720.0
29	43055.0
30	57363.0
31	74346.0
32	97361.0
33	132522.0
34	198053.0
35	359077.0
36	1157149.0
37	4410073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98661725223196	18.666873949098527	15.498346816540352	23.848161982129163
2	14.532128347013757	21.272454280024313	41.97576602257993	22.219651350382
3	18.124652156790784	26.437523382109546	31.17216096679843	24.265663494301243
4	12.464053241280538	17.648826285957934	39.46413551785985	30.42298495490168
5	13.223103930168852	37.41246370474012	35.86442153125048	13.500010833840554
6	29.41674713799607	38.15975946636031	19.183960868281225	13.2395325273624
7	25.881060189030062	32.68072702191663	24.260150967674093	17.178061821379213
8	23.866768913877937	37.11570738690645	21.85743241615403	17.160091283061586
9	25.97761016386979	16.457558391748947	21.69021246600788	35.87461897837338
10-11	24.252464888502338	26.525601960092065	29.432253636155636	19.789679515249958
12-13	24.971153952510807	24.278076049318067	29.493774784304293	21.25699521386683
14-15	22.194569412416428	25.40081800799462	27.104647778206587	25.29996480138237
16-17	21.73774499442474	28.287309208870536	27.817969053399622	22.156976743305105
18-19	21.779163600235226	27.576730486321253	29.460818695905612	21.183287217537913
20-21	22.63284672063732	26.859026770567347	29.221891934646095	21.28623457414924
22-23	22.36987203377137	26.709515274523817	29.100810349489688	21.819802342215127
24-25	22.0634844384826	26.879628634810445	29.206010362474455	21.850876564232504
26-27	21.94599077423287	26.961835744728766	29.205578138904226	21.886595342134132
28-29	21.979590161268792	27.045746381081447	29.15224174452505	21.822421713124715
30-31	22.24595035220829	26.90414764743927	29.04498421365547	21.80491778669697
32-33	22.005676108765023	27.120724933784945	28.969154219316678	21.904444738133353
34-35	22.190615822794378	27.043095856110728	29.017788923259268	21.748499397835626
36-37	22.171724561448713	26.95144126535405	28.989598938912632	21.887235234284606
38-39	21.979335109463687	27.143851113942624	29.07825012548747	21.79856365110622
40-41	22.25941279976878	27.00952659328629	28.893763586058835	21.8372970208861
42-43	22.12343240618279	27.104918988307514	28.725513601080156	22.046135004429544
44-45	22.13380481778387	27.252531999711916	28.61888982190329	21.994773360600924
46-47	22.25499078589078	27.111401751396514	28.41772355880943	22.215883903903276
48-49	22.219566168349473	27.21490851120072	28.379625847132356	22.18589947331745
50-51	22.127400645641497	27.40182113807334	28.294439362417183	22.17633885386798
52-53	22.33076084037974	27.31560411099045	28.121115091699593	22.232519956930222
54-55	22.339080641873576	27.177641292333142	28.183684336893894	22.29959372889939
56-57	22.269737704895984	27.344051064184654	28.164912809290122	22.22129842162924
58-59	22.268629399623926	27.32532337859435	28.207674470109183	22.198372751672537
60-61	22.32940068889769	27.25574830732082	28.26363068680473	22.151220316976758
62-63	22.333639810020312	27.292419881273972	28.276361000271887	22.097579308433822
64-65	22.22450311360665	27.206457824862312	28.295152993808724	22.273886067722316
66-67	22.28450258039376	27.21568543345945	28.212943169655674	22.286868816491122
68-69	22.241387751281188	27.233611051682725	28.306029851838314	22.21897134519777
70-71	22.23929896777714	26.90505956751672	28.291080565590526	22.564560899115612
72-73	22.329006573951837	26.993414291412897	28.338469104042918	22.339110030592348
74-75	22.166543178590512	27.280928189392313	28.238617235764625	22.313911396252553
76-77	22.304618291263154	27.22147716860494	28.035110807053627	22.438793733078278
78-79	22.249820553224204	27.173299575865183	28.018904740430468	22.557975130480145
80-81	22.32904745878107	27.282250613900988	27.95064613012686	22.43805579719108
82-83	22.455863790461652	27.210668921430937	28.03575088163015	22.297716406477264
84-85	22.49102721995367	27.232449226412207	27.909829121255758	22.366694432378363
86-87	22.277333169521224	27.327301699387235	27.94731986612362	22.448045264967924
88-89	22.428853623556176	27.297187628451415	27.817661618737144	22.456297129255265
90-91	22.468719139598527	27.355039883899412	27.86231234693369	22.313928629568366
92-93	22.56430605602122	27.32509026603295	27.80185485153181	22.30874882641402
94-95	22.35764885882703	27.319738210282924	27.85284268366238	22.46977024722766
96-97	22.45477249245312	27.403193906405793	27.936712459813783	22.205321141327307
98-99	23.524630474975297	29.383666657613322	28.12318483868866	18.968518028722723
100	30.13974789991037	40.43318970437448	29.427062395715154	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1257.0
1	1264.5
2	1895.5
3	2738.5
4	3458.0
5	4798.0
6	6019.5
7	7511.5
8	9061.5
9	9469.0
10	8920.0
11	8363.5
12	9068.5
13	11270.5
14	15390.0
15	20302.0
16	24105.5
17	26289.0
18	26151.5
19	24362.0
20	22736.5
21	22192.5
22	23571.0
23	27678.5
24	34981.5
25	45488.5
26	59680.0
27	75152.0
28	89183.0
29	104305.5
30	118299.0
31	129995.5
32	144887.5
33	162600.5
34	178560.5
35	192890.0
36	209384.5
37	223115.0
38	231786.5
39	239237.0
40	247495.0
41	255203.0
42	259612.0
43	264355.0
44	269666.5
45	274606.5
46	277007.0
47	278185.5
48	279389.0
49	278098.0
50	274487.0
51	268216.5
52	259040.5
53	246311.0
54	230847.0
55	214749.0
56	197071.0
57	177953.5
58	158699.0
59	136215.0
60	113819.0
61	92669.5
62	72643.5
63	56238.5
64	42385.0
65	31671.0
66	23434.5
67	17678.0
68	13378.0
69	9655.5
70	6987.0
71	4953.5
72	3400.5
73	2255.0
74	1474.5
75	948.0
76	633.5
77	414.0
78	270.0
79	174.5
80	108.5
81	67.5
82	43.5
83	33.5
84	24.5
85	15.5
86	13.0
87	11.0
88	5.5
89	2.5
90	1.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017273535988275815
3	0.001424309107805199
4	0.0
5	0.0
6	4.8487118563581233E-4
7	0.0
8	0.0
9	0.0
10-11	1.5152224551119135E-5
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.2729161658359033E-4
24-25	0.001106215476227278
26-27	0.0027355402449498216
28-29	0.0017435061408181394
30-31	0.0026030360203267515
32-33	0.0034541491749642966
34-35	8.548844356533829E-4
36-37	7.668588747312255E-6
38-39	5.405611194310332E-5
40-41	0.0
42-43	5.176138503427781E-4
44-45	0.0
46-47	0.0
48-49	2.6668218272093405E-4
50-51	0.006326057061687708
52-53	0.0
54-55	0.0027776458719400467
56-57	0.04551825053288788
58-59	0.05643180763038899
60-61	0.0371046332629387
62-63	0.008908950268018872
64-65	0.0
66-67	0.0
68-69	7.531855714750692E-4
70-71	0.0028862124361266664
72-73	0.009836125194862733
74-75	0.004579501020169776
76-77	0.0037217356032035872
78-79	0.0038442906241843372
80-81	0.01663722379292652
82-83	0.05358899321717015
84-85	0.03506226120752135
86-87	0.009413641196414772
88-89	0.008106729964014226
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	32.0
20-21	140.0
22-23	342.0
24-25	572.0
26-27	1797.0
28-29	4613.0
30-31	15892.0
32-33	19859.0
34-35	26560.0
36-37	42529.0
38-39	50449.0
40-41	48549.0
42-43	54973.0
44-45	64418.0
46-47	65925.0
48-49	62708.0
50-51	57978.0
52-53	55229.0
54-55	60848.0
56-57	63613.0
58-59	63077.0
60-61	61006.0
62-63	59133.0
64-65	61373.0
66-67	65173.0
68-69	67203.0
70-71	65835.0
72-73	62082.0
74-75	61694.0
76-77	64707.0
78-79	67468.0
80-81	66732.0
82-83	62278.0
84-85	61107.0
86-87	63421.0
88-89	65535.0
90-91	66269.0
92-93	65626.0
94-95	72963.0
96-97	202231.0
98-99	1455381.0
100-101	3022371.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.54249520939521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.52174932655662	77.43456308113043
2	6.56739379306385	11.235825041627525
3	1.5762532962005977	4.045099201170991
4	0.5787841617582695	1.9804256553792237
5	0.2873663891259879	1.2291018982575508
6	0.1449595772873754	0.744012236739676
7	0.0869618684301206	0.5207254649508554
8	0.053834580217117255	0.3684115456258044
9	0.042054478562900934	0.323770052790051
>10	0.1367326200614833	1.812989743401208
>50	0.0033712947188894293	0.18430077317286625
>100	4.8005316839499357E-4	0.07721821544467103
>500	5.856084840130728E-5	0.04355709030913996
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.545667365335741E-5	0.0	0.0	0.0
3	0.0	4.545667365335741E-5	0.0	0.0	0.0
4	0.0	1.2121779640895308E-4	0.0	1.5152224551119135E-5	0.0
5	0.0	1.2121779640895308E-4	0.0	1.5152224551119135E-5	0.0
6	0.0	1.2121779640895308E-4	0.0	1.5152224551119135E-5	0.0
7	1.5152224551119135E-5	1.3637002096007222E-4	0.0	1.5152224551119135E-5	0.0
8	1.5152224551119135E-5	1.8182669461342963E-4	0.0	1.5152224551119135E-5	0.0
9	7.576112275559568E-5	3.788056137779784E-4	0.0	1.5152224551119135E-5	0.0
10-11	9.848945958227439E-5	3.788056137779784E-4	0.0	2.2728336826678704E-5	0.0
12-13	1.0606557185783395E-4	3.939578383290975E-4	0.0	3.030444910223827E-5	0.0
14-15	1.0606557185783395E-4	4.8487118563581233E-4	0.0	3.030444910223827E-5	0.0
16-17	1.0606557185783395E-4	4.8487118563581233E-4	0.0	3.030444910223827E-5	0.0
18-19	1.1364168413339351E-4	5.454800838402889E-4	0.0	3.030444910223827E-5	0.0
20-21	1.3637002096007222E-4	5.757845329425271E-4	0.0	3.030444910223827E-5	0.0
22-23	1.6667447006231048E-4	6.13665094320325E-4	0.0	3.788056137779784E-5	0.0
24-25	1.6667447006231048E-4	6.666978802492419E-4	0.0	5.3032785928916975E-5	0.0
26-27	1.6667447006231048E-4	6.970023293514803E-4	0.0	1.1364168413339351E-4	0.0
28-29	1.6667447006231048E-4	6.970023293514803E-4	0.0	3.712295015024188E-4	0.0
30-31	1.818266946134296E-4	7.576112275559568E-4	0.0	0.0016288641392453072	0.0
32-33	1.9697891916454875E-4	7.87915676658195E-4	0.0	0.0042350467620377984	0.0
34-35	1.9697891916454875E-4	9.773184835471843E-4	0.0	0.007318524458190543	0.0
36-37	1.9697891916454875E-4	0.0011667212904361735	0.0	0.012568770265153323	0.0
38-39	2.121311437156679E-4	0.0012349063009162095	0.0	0.020349437572153	0.0
40-41	2.575878173690253E-4	0.0014091568832540796	0.0	0.028630128289339606	0.0
42-43	2.8789226647126355E-4	0.001454613556907437	0.0	0.03687293844514841	0.0
44-45	2.8789226647126355E-4	0.0016364402515208667	0.0	0.046494601035109065	0.0
46-47	2.8789226647126355E-4	0.001772810272480939	0.0	0.05659355869842997	0.0
48-49	3.0304449102238273E-4	0.001787962497032058	0.0	0.06717738754738668	0.0
50-51	3.4850116467574014E-4	0.001977365303921047	0.0	0.07784455363137456	0.0
52-53	3.4850116467574014E-4	0.0019849414161966067	0.0	0.08787532628421543	0.0
54-55	3.4850116467574014E-4	0.002000093640747726	0.0	0.09667876874841565	0.0
56-57	3.6365338922685926E-4	0.0020607025389522025	0.0	0.10405032599253511	0.0
58-59	3.788056137779784E-4	0.0020607025389522025	0.0	0.11152037269623684	0.0
60-61	3.788056137779784E-4	0.002068278651227762	0.0	0.11930104000323652	0.0
62-63	4.015339506046571E-4	0.0020910069880544406	0.0	0.12689988061562277	0.0
64-65	4.242622874313358E-4	0.0022879859072189894	0.0	0.13421082896153774	0.0
66-67	4.394145119824549E-4	0.0022879859072189894	0.0	0.14100660167271467	0.0
68-69	4.394145119824549E-4	0.0023107142440456684	0.0	0.14865847507102983	0.0
70-71	4.394145119824549E-4	0.002318290356321228	0.0	0.15490119158609092	0.0
72-73	4.469906242580145E-4	0.0023258664685967875	0.0	0.15976505566700017	0.0
74-75	4.545667365335741E-4	0.0023940514790768237	0.0	0.163242491201482	0.0
76-77	4.545667365335741E-4	0.002500117050934657	0.0	0.16673507896051498	0.0
78-79	4.545667365335741E-4	0.0025455737245880148	0.0	0.1692579243482763	0.0
80-81	4.697189610846932E-4	0.0025683020614146934	0.0	0.17005341613721006	0.0
82-83	4.697189610846932E-4	0.002598606510516932	0.0	0.17071253790518376	0.0
84-85	5.000234101869315E-4	0.0026213348473436105	0.0	0.17105346295758392	0.0
86-87	5.151756347380506E-4	0.0026364870718947296	0.0	0.1712655941012996	0.0
88	5.151756347380506E-4	0.0027425526437525635	0.0	0.1712655941012996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7480	0.0	50.647858	1
GTATCAA	14090	0.0	37.749447	1
TCAACGC	17480	0.0	29.948904	4
ATCAACG	17670	0.0	29.65076	3
CAACGCA	17670	0.0	29.532219	5
AACGCAG	18150	0.0	28.935503	6
TATCAAC	18475	0.0	28.744432	2
ACGCAGA	20985	0.0	24.986378	7
CGCAGAG	21555	0.0	24.228643	8
GTACATG	19790	0.0	21.129475	1
TACATGG	19380	0.0	21.101803	2
ACATGGG	19240	0.0	20.689651	3
GCAGAGT	25515	0.0	20.55023	9
GAGTACT	14955	0.0	18.998434	12-13
AGAGTAC	22715	0.0	18.941631	10-11
GTGGTAT	3925	0.0	18.21759	1
CAGAGTA	24710	0.0	18.139978	10-11
CATGGGG	13985	0.0	17.909454	4
AGTACTT	16395	0.0	17.215002	12-13
GTACTTT	17235	0.0	16.54599	14-15
>>END_MODULE