FastQCFastQC Report
Fri 27 May 2016
SRR2139159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7560070
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73250.046.055731
GTATCAA141650.037.9274941
TCAACGC157900.032.9542434
CAACGCA161700.032.231965
ATCAACG162550.031.9343453
AACGCAG167050.031.1996926
TATCAAC173600.030.2913062
ACGCAGA194450.026.8029797
CGCAGAG204150.025.5088088
GTACATG203950.023.3024441
TACATGG205050.022.2519862
ACATGGG202550.021.9429653
GCAGAGT247500.021.1431249
CATGGGG145700.018.638234
AGAGTAC215300.018.50803610-11
GAGTACT139650.018.26783212-13
CAGAGTA237250.017.90652810-11
GTGGTAT45300.016.75621
TGGTATC42650.015.8198452
GTACTTT162850.015.66550814-15
Produced by FastQC (version 0.11.5)