##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7560070
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86565560900891	33.0	32.0	34.0	28.0	34.0
2	32.19741576466885	33.0	33.0	34.0	29.0	34.0
3	31.97413820242405	33.0	31.0	33.0	29.0	34.0
4	32.562785397489705	33.0	33.0	34.0	31.0	34.0
5	32.85397701344035	33.0	33.0	34.0	31.0	34.0
6	36.58662089107641	38.0	37.0	38.0	34.0	38.0
7	37.06256833600747	38.0	38.0	38.0	36.0	38.0
8	37.0144736755083	38.0	38.0	38.0	36.0	38.0
9	37.12257108730475	38.0	38.0	38.0	36.0	38.0
10-11	37.229591987904875	38.0	38.0	38.0	36.0	38.0
12-13	37.30936380218702	38.0	38.0	38.0	37.0	38.0
14-15	37.27013896696724	38.0	38.0	38.0	37.0	38.0
16-17	37.33662942274344	38.0	38.0	38.0	37.0	38.0
18-19	37.3559505401405	38.0	38.0	38.0	37.0	38.0
20-21	37.37026017259846	38.0	38.0	38.0	37.0	38.0
22-23	37.380670275502126	38.0	38.0	38.0	37.0	38.0
24-25	37.394809830137916	38.0	38.0	38.0	37.0	38.0
26-27	37.36595095239915	38.0	38.0	38.0	37.0	38.0
28-29	37.346753129312134	38.0	38.0	38.0	37.0	38.0
30-31	37.342454584187436	38.0	38.0	38.0	37.0	38.0
32-33	37.32723122401455	38.0	38.0	38.0	37.0	38.0
34-35	37.30947112734623	38.0	38.0	38.0	37.0	38.0
36-37	37.292013935126775	38.0	38.0	38.0	37.0	38.0
38-39	37.28874957369046	38.0	38.0	38.0	37.0	38.0
40-41	37.280702334238995	38.0	38.0	38.0	37.0	38.0
42-43	37.240243237486	38.0	38.0	38.0	37.0	38.0
44-45	37.156239179165574	38.0	38.0	38.0	36.0	38.0
46-47	37.19014279972521	38.0	38.0	38.0	37.0	38.0
48-49	37.15317556110552	38.0	38.0	38.0	36.0	38.0
50-51	37.11084447389665	38.0	38.0	38.0	36.0	38.0
52-53	37.087091943348796	38.0	38.0	38.0	36.0	38.0
54-55	37.05323849100769	38.0	38.0	38.0	36.0	38.0
56-57	37.003040502394676	38.0	38.0	38.0	36.0	38.0
58-59	36.98452088010968	38.0	38.0	38.0	36.0	38.0
60-61	36.989055979102986	38.0	38.0	38.0	36.0	38.0
62-63	36.98928313818288	38.0	38.0	38.0	36.0	38.0
64-65	36.94774862241497	38.0	38.0	38.0	36.0	38.0
66-67	36.96884164596262	38.0	38.0	38.0	36.0	38.0
68-69	36.95029831861991	38.0	38.0	38.0	36.0	38.0
70-71	36.947701856676275	38.0	38.0	38.0	36.0	38.0
72-73	36.923973728428635	38.0	38.0	38.0	35.0	38.0
74-75	36.91039429836634	38.0	38.0	38.0	35.0	38.0
76-77	36.891697737018546	38.0	38.0	38.0	35.0	38.0
78-79	36.8631247376734	38.0	38.0	38.0	35.0	38.0
80-81	36.82397412772008	38.0	38.0	38.0	35.0	38.0
82-83	36.77961461603498	38.0	38.0	38.0	35.0	38.0
84-85	36.75327351630436	38.0	38.0	38.0	35.0	38.0
86-87	36.7420506716406	38.0	38.0	38.0	35.0	38.0
88-89	36.721468559995	38.0	38.0	38.0	34.5	38.0
90-91	36.69531581355396	38.0	38.0	38.0	34.0	38.0
92-93	36.6629919561038	38.0	38.0	38.0	34.0	38.0
94-95	36.6271512641608	38.0	38.0	38.0	34.0	38.0
96-97	36.61695980634521	38.0	38.0	38.0	34.0	38.0
98-99	36.622761158667224	38.0	38.0	38.0	34.0	38.0
100	35.12789627094207	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	19.0
21	89.0
22	384.0
23	1410.0
24	3393.0
25	7069.0
26	12770.0
27	21064.0
28	32088.0
29	46317.0
30	62732.0
31	83196.0
32	109613.0
33	149384.0
34	220012.0
35	381222.0
36	1004217.0
37	5425083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97967743684913	19.034307883392614	13.219414635049676	25.766600044708582
2	14.299760195335839	20.35519503358707	43.03690139939626	22.308143371680828
3	18.348563054298815	25.85750928353578	29.977874013758665	25.816053648406744
4	12.296169215364408	17.279694500183197	39.87652230733313	30.54761397711926
5	13.380643300921816	37.0361782364449	35.98399221171233	13.599186250920958
6	29.735301349954785	37.95264860686417	19.11887662894504	13.193173414236
7	26.15668902536617	32.28915869826602	24.187328953303343	17.36682332306447
8	23.69653984685327	37.33039508893436	21.808779548337515	17.16428551587485
9	25.709709037085638	16.543722478760117	21.607445433706303	36.13912305044795
10-11	24.115927498025812	26.51497935865673	29.503509888135955	19.865583255181498
12-13	24.81299114955285	24.165424394218572	29.562193207205755	21.459391249022826
14-15	22.101647206970306	25.384837706529172	26.850346623774644	25.66316846272587
16-17	21.802595743161106	28.39014056748152	27.600723273726302	22.20654041563107
18-19	21.89075630252101	27.74905523361556	29.072336631803676	21.287851832059758
20-21	22.538734065501483	27.077734797246357	28.863862914503308	21.51966822274885
22-23	22.34778642631428	26.90638587928843	28.729419564487845	22.016408129909447
24-25	22.12882780682463	26.985449851200606	28.811284214882026	22.074438127092737
26-27	22.024471620433683	27.12150760391217	28.8393394439288	22.014681331725345
28-29	22.02923821517408	27.093520046286518	28.865578001834734	22.01166373670467
30-31	22.24820824179962	27.06092842193148	28.696981339540308	21.993881996728597
32-33	22.014571564541598	27.230407139628564	28.68364757708082	22.071373718749022
34-35	22.174011568649046	27.167431288061223	28.700079296124603	21.958477847165124
36-37	22.18309508582396	27.096843471356273	28.61318186097733	22.106879581842428
38-39	21.980988307881955	27.255006441838308	28.747688346793833	22.016316903485908
40-41	22.241765272391685	27.081469091054807	28.662594732839246	22.014170903714263
42-43	22.097106515639048	27.16206388661887	28.478168857130587	22.262660740611494
44-45	22.11692453721633	27.283816444287712	28.403972667762194	22.195286350733763
46-47	22.19863497544813	27.241866947725747	28.26193581681546	22.297562260010668
48-49	22.112228294425282	27.335988214879634	28.21964823286197	22.332135257833112
50-51	22.14844252892812	27.426508712881276	28.13395292730737	22.291095830883233
52-53	22.298953661117558	27.297267166714335	28.057461197272115	22.34631797489599
54-55	22.282219490706733	27.204936721114727	28.093939205044734	22.41890458313381
56-57	22.17279424606663	27.306703438472034	28.101262281887834	22.4192400335735
58-59	22.15489743662737	27.266084444444772	28.11796114256141	22.461056976366454
60-61	22.152616576029025	27.289771976338123	28.188645739735296	22.368965707897555
62-63	22.139239909214727	27.313758530525384	28.231545732095075	22.315455828164808
64-65	22.081406422712966	27.24152577025861	28.220719846447633	22.45634796058079
66-67	22.106657685856344	27.307945480427776	28.02708320684707	22.558313626868813
68-69	22.026353141902092	27.346061583225683	28.14011070674209	22.487474568130136
70-71	22.11160161863324	27.078840075143702	28.216403630448667	22.59315467577439
72-73	22.213360291410993	27.177665473487256	28.05224392159579	22.556730313505962
74-75	22.118182817140276	27.316497311794087	28.000809228845103	22.564510642220533
76-77	22.31692022553741	27.22572210442437	27.853870082902983	22.603487587135238
78-79	22.2669731006538	27.298038564508285	27.756652750762616	22.678335584075292
80-81	22.319726235784202	27.35900001017016	27.686421337561473	22.63485241648416
82-83	22.415423594849443	27.341328462120163	27.719024502036948	22.524223440993442
84-85	22.424071845143022	27.34419560081896	27.673102768637463	22.55862978540055
86-87	22.343696758309324	27.431216558752077	27.755423353487185	22.469663329451414
88-89	22.428859307256314	27.423712024462443	27.680859089139336	22.466569579141904
90-91	22.489707724453446	27.41409024291838	27.651874036714723	22.444327995913447
92-93	22.5555892939719	27.456658226908655	27.61123746167855	22.376515017440898
94-95	22.419986057828986	27.40769959876686	27.732049093012662	22.440265250391498
96-97	22.543628481023273	27.510244306225935	27.770707761459	22.175419451291795
98-99	23.576002172804916	29.407643954069428	28.005639383707337	19.010714489418316
100	30.072344688809988	40.48867432402133	29.438980987168677	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1111.0
1	1085.5
2	1628.0
3	2346.5
4	2922.0
5	4301.5
6	5591.0
7	6828.0
8	8020.0
9	8171.0
10	7527.0
11	7075.5
12	7695.5
13	9509.5
14	13026.0
15	17269.0
16	20638.5
17	22865.5
18	23231.5
19	21918.5
20	20641.5
21	20444.0
22	22063.0
23	26276.5
24	33498.5
25	44048.5
26	58094.5
27	74012.0
28	90449.5
29	109177.0
30	127743.0
31	145025.5
32	165027.0
33	186244.0
34	204861.0
35	223602.0
36	244624.5
37	260481.0
38	273779.0
39	285923.0
40	295525.5
41	303481.0
42	308122.0
43	313408.5
44	319276.0
45	323178.0
46	322936.0
47	321088.5
48	319522.5
49	316490.0
50	310969.0
51	302794.0
52	291869.0
53	277585.5
54	258732.5
55	239428.5
56	218908.5
57	196199.5
58	173881.0
59	149364.0
60	125420.5
61	102805.0
62	81804.5
63	63310.5
64	47012.5
65	34788.5
66	25671.0
67	19361.0
68	14869.5
69	10875.5
70	7971.0
71	5756.0
72	3997.0
73	2685.5
74	1788.5
75	1167.5
76	771.5
77	479.5
78	292.0
79	173.0
80	108.5
81	72.0
82	55.0
83	45.5
84	35.5
85	23.0
86	12.5
87	8.5
88	8.5
89	7.5
90	5.5
91	3.0
92	2.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00220897425552938
3	0.002394157726052801
4	0.0
5	0.0
6	5.158682393152444E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.3810131660768587E-4
24-25	0.0010715108481544118
26-27	0.002553347938571741
28-29	0.0015681800000674911
30-31	0.0020199983813521164
32-33	0.003086256898033059
34-35	7.054410334152111E-4
36-37	1.3364105522709925E-5
38-39	4.0337105256045826E-5
40-41	6.7688066045682805E-6
42-43	4.975462450491592E-4
44-45	0.0
46-47	0.0
48-49	1.8200708735598164E-4
50-51	0.006005647852549899
52-53	0.0
54-55	0.0024919111630079737
56-57	0.04424456319938898
58-59	0.05607631938064009
60-61	0.03560815950741184
62-63	0.008232036863404388
64-65	0.0
66-67	0.0
68-69	4.545856247851794E-4
70-71	0.002510340341340799
72-73	0.009337808225552234
74-75	0.004553100434139323
76-77	0.0034356900123684838
78-79	0.003314366882454264
80-81	0.015409967553916491
82-83	0.05246677125417016
84-85	0.034007173531134104
86-87	0.00901945956975806
88-89	0.00812662814737536
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	36.0
20-21	148.0
22-23	369.0
24-25	555.0
26-27	1577.0
28-29	4251.0
30-31	15412.0
32-33	18958.0
34-35	26127.0
36-37	43271.0
38-39	50021.0
40-41	50322.0
42-43	55194.0
44-45	66260.0
46-47	68197.0
48-49	66886.0
50-51	59773.0
52-53	55329.0
54-55	60278.0
56-57	63576.0
58-59	69176.0
60-61	68526.0
62-63	65613.0
64-65	68301.0
66-67	73600.0
68-69	75778.0
70-71	75157.0
72-73	69728.0
74-75	69918.0
76-77	73663.0
78-79	76713.0
80-81	76920.0
82-83	71383.0
84-85	69508.0
86-87	72063.0
88-89	75985.0
90-91	77313.0
92-93	76268.0
94-95	85691.0
96-97	236886.0
98-99	1701930.0
100-101	3523410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.54304255238608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68944318040145	78.48540340213684
2	6.589419548778444	11.405368328109152
3	1.5519081075798706	4.029205481750331
4	0.5482075429474204	1.897741948673505
5	0.2479716369341234	1.0730109963487338
6	0.12481925901685331	0.6481343066671702
7	0.07565976435956569	0.45834783445313787
8	0.04638378357591558	0.3211355004600903
9	0.02947320498376346	0.22956307497585351
>10	0.09429491371821022	1.25693412337989
>50	0.00196404337196213	0.11586933088797398
>100	4.0759616428472957E-4	0.05259103531441001
>500	4.741816812496244E-5	0.026694636842886088
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9682172255018806E-5
2	0.0	3.9682172255018806E-5	0.0	1.3227390751672934E-5	3.9682172255018806E-5
3	0.0	3.9682172255018806E-5	0.0	1.3227390751672934E-5	3.9682172255018806E-5
4	0.0	1.0581912601338347E-4	0.0	1.3227390751672934E-5	3.9682172255018806E-5
5	0.0	1.190465167650564E-4	0.0	1.3227390751672934E-5	3.9682172255018806E-5
6	1.3227390751672935E-4	1.190465167650564E-4	0.0	1.3227390751672934E-5	5.2909563006691734E-5
7	1.3227390751672935E-4	1.190465167650564E-4	0.0	2.6454781503345867E-5	5.2909563006691734E-5
8	1.3227390751672935E-4	1.3227390751672935E-4	0.0	2.6454781503345867E-5	5.2909563006691734E-5
9	1.7195607977174814E-4	2.380930335301128E-4	0.0	2.6454781503345867E-5	6.613695375836468E-5
10-11	1.8518347052342107E-4	2.380930335301128E-4	0.0	6.613695375836468E-5	6.613695375836468E-5
12-13	1.9841086127509402E-4	2.5132042428178573E-4	0.0	6.613695375836468E-5	7.275064913420115E-5
14-15	1.9841086127509402E-4	3.1745737804015045E-4	0.0	6.613695375836468E-5	9.9205430637547E-5
16-17	2.579341196576222E-4	3.1745737804015045E-4	0.0	7.936434451003761E-5	1.190465167650564E-4
18-19	2.645478150334587E-4	3.439121595434963E-4	0.0	7.936434451003761E-5	1.4550129826840227E-4
20-21	2.645478150334587E-4	3.835943317985151E-4	0.0	8.597803988587407E-5	1.4550129826840227E-4
22-23	2.645478150334587E-4	4.2989019942937036E-4	0.0	9.259173526171053E-5	1.8518347052342107E-4
24-25	2.645478150334587E-4	4.827997624360621E-4	0.0	9.259173526171053E-5	2.2486564277843989E-4
26-27	3.0422998728847747E-4	4.894134578118986E-4	0.0	1.2566021214089286E-4	2.2486564277843989E-4
28-29	3.0422998728847747E-4	4.894134578118986E-4	0.0	3.968217225501881E-4	2.2486564277843989E-4
30-31	3.1084368266431396E-4	6.084599745769549E-4	0.0	0.0018452210098583743	2.2486564277843989E-4
32-33	3.2407107341598693E-4	6.084599745769549E-4	0.0	0.004880907187367313	2.380930335301128E-4
34-35	3.3068476879182337E-4	7.07665405214502E-4	0.0	0.008577962902459896	2.645478150334587E-4
36-37	3.3068476879182337E-4	8.06870835852049E-4	0.0	0.0159059373788867	2.97616291912641E-4
38-39	3.3068476879182337E-4	8.597803988587407E-4	6.613695375836467E-6	0.037810496463657084	3.1745737804015045E-4
40-41	3.3068476879182337E-4	9.126899618654324E-4	1.3227390751672934E-5	0.06193725719470851	3.1745737804015045E-4
42-43	3.439121595434963E-4	9.457584387446147E-4	1.3227390751672934E-5	0.07549533271517328	3.1745737804015045E-4
44-45	3.439121595434963E-4	0.0010185090878788161	1.3227390751672934E-5	0.09187084246574437	3.1745737804015045E-4
46-47	3.439121595434963E-4	0.0010714186508855076	1.3227390751672934E-5	0.10996591301403294	3.1745737804015045E-4
48-49	3.439121595434963E-4	0.001091259737013017	1.3227390751672934E-5	0.1275649564091338	3.3068476879182337E-4
50-51	3.769806364226786E-4	0.0012235336445297464	1.3227390751672934E-5	0.14561373108979148	3.372984641676598E-4
52-53	3.9682172255018803E-4	0.0012301473399055829	1.3227390751672934E-5	0.20233278263296506	3.571395502951692E-4
54-55	3.9682172255018803E-4	0.0012367610352814193	1.3227390751672934E-5	0.2745344950509717	3.769806364226786E-4
56-57	4.1004911330186095E-4	0.0012566021214089288	1.3227390751672934E-5	0.3739581776359214	3.9682172255018803E-4
58-59	4.232765040535339E-4	0.001276443207536438	1.3227390751672934E-5	0.4328531349577451	4.1004911330186095E-4
60-61	4.629586763085527E-4	0.00132935277054313	1.3227390751672934E-5	0.45420875732632104	4.232765040535339E-4
62-63	4.960271531877351E-4	0.0013888760289256581	1.3227390751672934E-5	0.4719665294104419	4.232765040535339E-4
64-65	5.026408485635715E-4	0.0014682403734356958	1.3227390751672934E-5	0.48152331922852565	4.232765040535339E-4
66-67	5.09254543939408E-4	0.0014814677641873685	1.3227390751672934E-5	0.49022694234312647	4.232765040535339E-4
68-69	5.224819346910809E-4	0.001514536241066551	1.3227390751672934E-5	0.4995919349953109	4.232765040535339E-4
70-71	5.357093254427538E-4	0.0015476047179457334	1.3227390751672934E-5	0.5058484908208523	4.3650389480520685E-4
72-73	5.621641069460997E-4	0.0015476047179457334	1.3227390751672934E-5	0.5107624664850987	4.4973128555687977E-4
74-75	5.687778023219361E-4	0.0015872868902007521	1.3227390751672934E-5	0.5150018452210099	4.4973128555687977E-4
76-77	5.687778023219361E-4	0.0016468101485832805	1.3227390751672934E-5	0.5189369939696326	4.4973128555687977E-4
78-79	5.753914976977726E-4	0.0016931060162141355	1.3227390751672934E-5	0.5215692447292155	4.4973128555687977E-4
80-81	5.886188884494456E-4	0.001699719711589972	1.3227390751672934E-5	0.5223562744789401	4.4973128555687977E-4
82-83	5.952325838252821E-4	0.0017195607977174814	1.3227390751672934E-5	0.5230308714072753	4.629586763085527E-4
84-85	6.349147560803009E-4	0.0017460155792208274	1.3227390751672934E-5	0.5232623507454296	4.629586763085527E-4
86-87	6.481421468319738E-4	0.0017724703607241731	1.3227390751672934E-5	0.5234739889974565	4.629586763085527E-4
88	6.481421468319738E-4	0.0018915168774892297	1.3227390751672934E-5	0.523487216388208	4.629586763085527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7325	0.0	46.05573	1
GTATCAA	14165	0.0	37.927494	1
TCAACGC	15790	0.0	32.954243	4
CAACGCA	16170	0.0	32.23196	5
ATCAACG	16255	0.0	31.934345	3
AACGCAG	16705	0.0	31.199692	6
TATCAAC	17360	0.0	30.291306	2
ACGCAGA	19445	0.0	26.802979	7
CGCAGAG	20415	0.0	25.508808	8
GTACATG	20395	0.0	23.302444	1
TACATGG	20505	0.0	22.251986	2
ACATGGG	20255	0.0	21.942965	3
GCAGAGT	24750	0.0	21.143124	9
CATGGGG	14570	0.0	18.63823	4
AGAGTAC	21530	0.0	18.508036	10-11
GAGTACT	13965	0.0	18.267832	12-13
CAGAGTA	23725	0.0	17.906528	10-11
GTGGTAT	4530	0.0	16.7562	1
TGGTATC	4265	0.0	15.819845	2
GTACTTT	16285	0.0	15.665508	14-15
>>END_MODULE