FastQCFastQC Report
Fri 27 May 2016
SRR2139158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6768971
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.047.466611
GTATCAA131000.037.076611
TCAACGC150700.031.6080044
CAACGCA153500.031.0314355
ATCAACG153500.030.9496823
AACGCAG158550.030.150066
TATCAAC161650.029.6529922
ACGCAGA183600.025.943857
CGCAGAG189600.025.0780988
GTACATG198300.023.3382261
TACATGG197650.022.9428142
ACATGGG193100.022.449943
GCAGAGT227300.021.0119469
CATGGGG141600.019.2909284
GAGTACT125250.018.60875312-13
CAGAGTA217950.018.51736610-11
AGAGTAC199850.018.14634910-11
ATGGGGG83750.016.7132035
GTGGTAT40250.016.6516421
CTCGATA7655.5842975E-1016.32582994
Produced by FastQC (version 0.11.5)