##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6768971
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.756841918808636	33.0	32.0	34.0	27.0	34.0
2	32.130070582367686	33.0	32.0	34.0	29.0	34.0
3	31.933854643490125	33.0	31.0	33.0	29.0	34.0
4	32.51817964650757	33.0	33.0	34.0	31.0	34.0
5	32.819617486911966	33.0	33.0	34.0	31.0	34.0
6	36.48955077514736	38.0	37.0	38.0	34.0	38.0
7	37.001103121877755	38.0	38.0	38.0	35.0	38.0
8	36.93370277402577	38.0	38.0	38.0	35.0	38.0
9	37.05905003877251	38.0	38.0	38.0	36.0	38.0
10-11	37.17154416823473	38.0	38.0	38.0	36.0	38.0
12-13	37.255630804150286	38.0	38.0	38.0	36.0	38.0
14-15	37.22139938256494	38.0	38.0	38.0	36.0	38.0
16-17	37.293414538191996	38.0	38.0	38.0	36.5	38.0
18-19	37.31591197539478	38.0	38.0	38.0	37.0	38.0
20-21	37.3326664689984	38.0	38.0	38.0	37.0	38.0
22-23	37.34646616469189	38.0	38.0	38.0	37.0	38.0
24-25	37.35945564357012	38.0	38.0	38.0	37.0	38.0
26-27	37.32863063566634	38.0	38.0	38.0	37.0	38.0
28-29	37.30933033771851	38.0	38.0	38.0	37.0	38.0
30-31	37.30564169345681	38.0	38.0	38.0	37.0	38.0
32-33	37.28965957502935	38.0	38.0	38.0	37.0	38.0
34-35	37.26947867643404	38.0	38.0	38.0	37.0	38.0
36-37	37.25132024875452	38.0	38.0	38.0	37.0	38.0
38-39	37.25114341087853	38.0	38.0	38.0	37.0	38.0
40-41	37.239417778598394	38.0	38.0	38.0	37.0	38.0
42-43	37.19817018645851	38.0	38.0	38.0	36.0	38.0
44-45	37.11734964421235	38.0	38.0	38.0	36.0	38.0
46-47	37.14478283222226	38.0	38.0	38.0	36.0	38.0
48-49	37.104952896183306	38.0	38.0	38.0	36.0	38.0
50-51	37.05935132550309	38.0	38.0	38.0	36.0	38.0
52-53	37.034100528484124	38.0	38.0	38.0	36.0	38.0
54-55	36.99796486333722	38.0	38.0	38.0	36.0	38.0
56-57	36.94290791811862	38.0	38.0	38.0	36.0	38.0
58-59	36.92202518783395	38.0	38.0	38.0	36.0	38.0
60-61	36.92517359776691	38.0	38.0	38.0	36.0	38.0
62-63	36.92515345369792	38.0	38.0	38.0	36.0	38.0
64-65	36.88296192906193	38.0	38.0	38.0	35.0	38.0
66-67	36.901044011729105	38.0	38.0	38.0	35.0	38.0
68-69	36.880048940983684	38.0	38.0	38.0	35.0	38.0
70-71	36.87908807865712	38.0	38.0	38.0	35.0	38.0
72-73	36.855877119577855	38.0	38.0	38.0	35.0	38.0
74-75	36.84108685946439	38.0	38.0	38.0	35.0	38.0
76-77	36.82034175514815	38.0	38.0	38.0	35.0	38.0
78-79	36.791993421844325	38.0	38.0	38.0	35.0	38.0
80-81	36.75065346816088	38.0	38.0	38.0	35.0	38.0
82-83	36.708199601042054	38.0	38.0	38.0	34.5	38.0
84-85	36.677921875991785	38.0	38.0	38.0	34.0	38.0
86-87	36.66396482470114	38.0	38.0	38.0	34.0	38.0
88-89	36.641028080603796	38.0	38.0	38.0	34.0	38.0
90-91	36.613629705261744	38.0	38.0	38.0	34.0	38.0
92-93	36.57868154010861	38.0	38.0	38.0	34.0	38.0
94-95	36.54894192334138	38.0	38.0	38.0	34.0	38.0
96-97	36.540273496675006	38.0	38.0	38.0	34.0	38.0
98-99	36.550043608037385	38.0	38.0	38.0	34.0	38.0
100	35.05477614587096	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	5.0
20	23.0
21	97.0
22	407.0
23	1358.0
24	3397.0
25	7052.0
26	12580.0
27	20578.0
28	31446.0
29	44485.0
30	59992.0
31	79168.0
32	104249.0
33	141108.0
34	209192.0
35	364180.0
36	946363.0
37	4743287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25942170530794	18.837530844791623	13.192226706245307	25.710820743655127
2	14.583332102193655	20.123962961802956	42.77663265372453	22.516072282278856
3	18.542539453523947	25.673167142059572	29.810245173913728	25.97404823050275
4	12.560963845169375	17.089953554240374	39.3882762978302	30.96080630276005
5	13.639133629025741	36.96505717043255	35.588688443191735	13.807120757349972
6	30.23667235145967	37.57170994082379	18.781755360074314	13.409862347642228
7	26.60419139039006	32.30715569619075	23.632040970481334	17.456611942937855
8	24.14615160856798	37.15001881379016	21.363424366864624	17.340405210777234
9	25.96109512066162	16.613692095888723	21.316356651550137	36.108856131899515
10-11	24.606858605751054	26.331079445537547	28.981105767856423	20.080956180854976
12-13	25.251282654335494	23.989111491244387	29.083615515563594	21.675990338856526
14-15	22.486349845493503	25.235268403424982	26.48313163108543	25.795250119996084
16-17	22.13221921027583	28.163025074269044	27.25276855226592	22.451987163189205
18-19	22.137515436245774	27.531969039311882	28.7857120380631	21.544803486379244
20-21	22.940188127861706	26.775275535621923	28.539969599227767	21.744566737288608
22-23	22.71116815483061	26.620559746627393	28.44058024543163	22.22769185311037
24-25	22.437990401206577	26.749978742647745	28.512939060360758	22.29909179578492
26-27	22.310857965111136	26.879964796248164	28.534847007972928	22.274330230667765
28-29	22.336604300785535	26.972012472338648	28.51283007107424	22.17855315580158
30-31	22.59682376378421	26.841628994086825	28.34374613722443	22.217801104904535
32-33	22.32489668847173	27.05151307377277	28.36325630524619	22.26033393250931
34-35	22.530952043709558	27.02483758360853	28.31602392961835	22.128186443063562
36-37	22.482746581581385	26.880426779185342	28.31248848342247	22.324338155810807
38-39	22.33919299346889	27.107884320865256	28.401154879503963	22.151767806161885
40-41	22.599972301549435	26.877585685851546	28.309870450087736	22.21257156251128
42-43	22.43746116795394	26.9817995268903	28.137790824711907	22.44294848044385
44-45	22.483428591207034	27.087332829055473	28.04285463335533	22.386383946382164
46-47	22.571567370572723	27.036921035891037	27.844658991053393	22.54685260248285
48-49	22.48369240152164	27.12295384763132	27.84462661307466	22.54872713777238
50-51	22.426473160976897	27.274344857074272	27.761914657750925	22.537267324197906
52-53	22.64906915761791	27.147646795215945	27.621561594186822	22.58172245297932
54-55	22.645457535280837	27.00936923295698	27.68573486515125	22.65943836661093
56-57	22.560839983008155	27.178848519694615	27.705561897018878	22.554749600278353
58-59	22.518042822803448	27.151920015192587	27.729113119735278	22.60092404226869
60-61	22.604920015778283	27.111617254512794	27.789217143723505	22.494245585985418
62-63	22.58395650326698	27.16922690067899	27.72856307705831	22.51825351899572
64-65	22.532595188909525	27.16506838754854	27.69378754984304	22.608548873698894
66-67	22.582554813248375	27.241251838536535	27.460488807182713	22.715704541032373
68-69	22.524219038233742	27.150890333188137	27.732900640133785	22.591989988444336
70-71	22.528302849144577	26.899204275569232	27.775295512571873	22.797197362714318
72-73	22.635897936028464	27.020472528706907	27.65892528585	22.68470424941463
74-75	22.458042874456684	27.20184978988456	27.649917237909786	22.690190097748978
76-77	22.600579521179647	27.06759901456872	27.50127124616249	22.830550218089144
78-79	22.57349053392536	27.06828995292393	27.456837352309932	22.90138216084078
80-81	22.598831529783844	27.17928896725243	27.406287437563147	22.81559206540058
82-83	22.716118698936988	27.072422354407642	27.519567187062794	22.691891759592576
84-85	22.768735088459717	27.103937306941646	27.386883406007378	22.74044419859126
86-87	22.540037087104686	27.19638916324088	27.492980719762812	22.77059302989162
88-89	22.630338874607343	27.19697338560405	27.414889546034466	22.75779819375414
90-91	22.68861661937003	27.16044271307485	27.45567077728402	22.695269890271096
92-93	22.72644268825185	27.130419675085417	27.44363288323356	22.699504753429174
94-95	22.544390553332008	27.165228412837454	27.528398489969252	22.761982543861286
96-97	22.673757842830454	27.25691934674515	27.581125670637036	22.488197139787356
98-99	23.748095761883018	29.230157144886466	27.729690984129853	19.292056109100667
100	30.5772531768972	40.303992034503075	29.118754788599727	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1137.0
1	1091.0
2	1481.5
3	2094.0
4	2671.5
5	3912.5
6	5046.5
7	6124.5
8	7187.0
9	7430.0
10	7002.5
11	6598.5
12	7027.0
13	8611.5
14	12054.0
15	16313.5
16	19570.5
17	21546.5
18	21907.5
19	20671.0
20	19073.0
21	18408.5
22	19586.5
23	23310.5
24	29498.0
25	38223.0
26	50638.5
27	64473.0
28	76737.0
29	90246.0
30	103980.0
31	117648.5
32	133761.0
33	150472.5
34	166462.5
35	182810.5
36	201892.0
37	217900.0
38	228988.0
39	237957.5
40	246098.0
41	253416.5
42	259104.0
43	265369.5
44	271952.5
45	277780.0
46	281663.5
47	284976.5
48	287102.5
49	286495.0
50	285085.5
51	281918.5
52	274698.0
53	263858.5
54	249287.0
55	232735.5
56	214515.5
57	194093.0
58	173623.5
59	150448.5
60	125772.5
61	102385.5
62	80937.5
63	61963.0
64	45620.5
65	33536.5
66	24358.0
67	18039.0
68	13587.0
69	9794.5
70	6998.5
71	4843.5
72	3174.5
73	2016.5
74	1308.5
75	829.0
76	517.5
77	304.5
78	179.5
79	124.0
80	92.5
81	60.0
82	36.5
83	26.5
84	17.0
85	9.0
86	5.0
87	3.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0025853264846311206
3	0.0027182861324121493
4	0.0
5	0.0
6	5.170652969262241E-4
7	0.0
8	0.0
9	0.0
10-11	7.386647098946059E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.58542808410176E-4
24-25	0.001196750600499163
26-27	0.002881352338339326
28-29	0.0017662749674791087
30-31	0.002374475673607681
32-33	0.0035430429313032144
34-35	0.0011444184778699191
36-37	1.4920260161592384E-5
38-39	8.253634487946767E-5
40-41	0.0
42-43	5.700084361248546E-4
44-45	0.0
46-47	0.0
48-49	1.4031207119559214E-4
50-51	0.005888861073826348
52-53	0.0
54-55	0.0028423954782917232
56-57	0.04773353863264187
58-59	0.06009035327212936
60-61	0.037323301722112394
62-63	0.00805289185719384
64-65	0.0
66-67	0.0
68-69	4.261233741369191E-4
70-71	0.0024726311181573917
72-73	0.00937724717631236
74-75	0.004669991243106816
76-77	0.0036256527730112093
78-79	0.0032887826094926487
80-81	0.015772659880453236
82-83	0.05487383982797921
84-85	0.03388735990319278
86-87	0.009220635424910566
88-89	0.007882969941731634
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	39.0
20-21	158.0
22-23	351.0
24-25	517.0
26-27	1474.0
28-29	3934.0
30-31	13134.0
32-33	16232.0
34-35	22405.0
36-37	36538.0
38-39	42547.0
40-41	41887.0
42-43	46606.0
44-45	56431.0
46-47	58414.0
48-49	55712.0
50-51	50394.0
52-53	47082.0
54-55	52395.0
56-57	54617.0
58-59	56871.0
60-61	56372.0
62-63	54410.0
64-65	57126.0
66-67	60748.0
68-69	63130.0
70-71	62452.0
72-73	57525.0
74-75	57955.0
76-77	61291.0
78-79	63999.0
80-81	64140.0
82-83	59474.0
84-85	58203.0
86-87	60407.0
88-89	64581.0
90-91	65047.0
92-93	63969.0
94-95	72265.0
96-97	213396.0
98-99	1574856.0
100-101	3219887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.69123839500126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32172229071566	74.75445554084382
2	7.275218686805414	12.177441229863998
3	1.8573555653011553	4.66333162199704
4	0.6865047919535463	2.2981774481077997
5	0.3273462528253733	1.3698006641459335
6	0.16684887218505706	0.8378273242786024
7	0.09860435306341706	0.5776624293310717
8	0.058974313037526246	0.3948506633284032
9	0.04670457078213001	0.3517887030717108
>10	0.15414725837373713	2.045024290247796
>50	0.005448298066173332	0.3093445723430631
>100	0.0010306145495361369	0.1553015825437767
>500	6.275489570836855E-5	0.03697432550887962
>1k	3.137744561248767E-5	0.028019604388066124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9546588395784235E-5	0.0	0.0	0.0
3	0.0	2.9546588395784235E-5	0.0	0.0	0.0
4	0.0	1.1818635358313694E-4	0.0	1.4773294197892118E-5	0.0
5	0.0	1.1818635358313694E-4	0.0	1.4773294197892118E-5	0.0
6	0.0	1.1818635358313694E-4	0.0	1.4773294197892118E-5	0.0
7	0.0	1.1818635358313694E-4	0.0	1.4773294197892118E-5	0.0
8	1.4773294197892118E-5	1.3295964778102905E-4	0.0	1.4773294197892118E-5	0.0
9	1.4773294197892118E-5	3.397857665515187E-4	0.0	1.4773294197892118E-5	0.0
10-11	1.4773294197892118E-5	3.397857665515187E-4	0.0	2.2159941296838176E-5	0.0
12-13	1.4773294197892118E-5	3.397857665515187E-4	0.0	2.9546588395784235E-5	0.0
14-15	2.2159941296838176E-5	3.914922962441411E-4	0.0	4.431988259367635E-5	0.0
16-17	2.9546588395784235E-5	3.9887894334308716E-4	0.0	5.909317679156847E-5	0.0
18-19	2.9546588395784235E-5	4.5058547303570954E-4	0.0	5.909317679156847E-5	0.0
20-21	2.9546588395784235E-5	5.02292002728332E-4	0.0	7.386647098946058E-5	0.0
22-23	2.9546588395784235E-5	5.835451208167385E-4	0.0	7.386647098946058E-5	0.0
24-25	7.386647098946058E-5	6.647982389051453E-4	0.0	7.386647098946058E-5	0.0
26-27	1.0341305938524482E-4	6.943448273009295E-4	0.0	1.6250623617681327E-4	0.0
28-29	1.0341305938524482E-4	7.017314743998756E-4	0.0	5.835451208167387E-4	0.0
30-31	1.3295964778102905E-4	9.011709460714191E-4	0.0	0.0018762083631322988	0.0
32-33	1.403462948799751E-4	9.307175344672033E-4	0.0	0.004786547320117046	0.0
34-35	1.6250623617681327E-4	0.0010784504764461246	0.0	0.008583283928975319	0.0
36-37	1.772795303747054E-4	0.0012261834184250457	0.0	0.014285775489361677	0.0
38-39	1.772795303747054E-4	0.001270503301018722	0.0	0.029842054279742077	0.0
40-41	1.772795303747054E-4	0.001403462948799751	0.0	0.04589323842575187	0.0
42-43	1.772795303747054E-4	0.0014551694784923734	0.0	0.05608681142229742	0.0
44-45	1.8466617747365147E-4	0.0016324490088670787	0.0	0.0682747791355584	0.0
46-47	1.9205282457259752E-4	0.001758022009549162	0.0	0.08134914450069294	0.0
48-49	1.9205282457259752E-4	0.001758022009549162	0.0	0.09463033598459795	0.0
50-51	1.9205282457259752E-4	0.0018762083631322988	0.0	0.109137710886928	0.0
52-53	1.9205282457259752E-4	0.0018762083631322988	0.0	0.1431532207775746	0.0
54-55	1.9205282457259752E-4	0.0018762083631322988	0.0	0.18369852670368952	0.0
56-57	1.9205282457259752E-4	0.0018762083631322988	0.0	0.23562665580928033	0.0
58-59	1.9205282457259752E-4	0.001890981657330191	0.0	0.275795242733349	0.0
60-61	1.9205282457259752E-4	0.0019426881870228132	0.0	0.2892684870418266	0.0
62-63	1.9205282457259752E-4	0.0020387145993091124	0.0	0.3048616990677017	0.0
64-65	1.9205282457259752E-4	0.002260314012277494	0.0	0.3121670930485594	0.0
66-67	1.9205282457259752E-4	0.002289860600673278	0.0	0.31934691402873494	0.0
68-69	1.9205282457259752E-4	0.0023637270716627386	0.0	0.32629774894884317	0.0
70-71	2.0682611877048963E-4	0.002408046954256415	0.0	0.3321110402157137	0.0
72-73	2.0682611877048963E-4	0.002408046954256415	0.0	0.3368089477706434	0.0
74-75	2.0682611877048963E-4	0.0024597534839490374	0.0	0.3406278443207985	0.0
76-77	2.0682611877048963E-4	0.0025631665433342825	0.0	0.3444171942825579	0.0
78-79	2.2159941296838174E-4	0.0026000997788290124	0.0	0.34700252076718896	0.0
80-81	2.2159941296838174E-4	0.0026074864259279587	0.0	0.34781505194807305	0.0
82-83	2.2898606006732783E-4	0.0026148730730269046	0.0	0.3483173439508014	0.0
84-85	2.3637270716627388E-4	0.002629646367224797	0.0	0.34855371665796764	0.0
86-87	2.3637270716627388E-4	0.002659192955620581	0.0	0.34866451636445184	0.0
88	2.3637270716627388E-4	0.002880792368588963	0.0	0.34866451636445184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7060	0.0	47.46661	1
GTATCAA	13100	0.0	37.07661	1
TCAACGC	15070	0.0	31.608004	4
CAACGCA	15350	0.0	31.031435	5
ATCAACG	15350	0.0	30.949682	3
AACGCAG	15855	0.0	30.15006	6
TATCAAC	16165	0.0	29.652992	2
ACGCAGA	18360	0.0	25.94385	7
CGCAGAG	18960	0.0	25.078098	8
GTACATG	19830	0.0	23.338226	1
TACATGG	19765	0.0	22.942814	2
ACATGGG	19310	0.0	22.44994	3
GCAGAGT	22730	0.0	21.011946	9
CATGGGG	14160	0.0	19.290928	4
GAGTACT	12525	0.0	18.608753	12-13
CAGAGTA	21795	0.0	18.517366	10-11
AGAGTAC	19985	0.0	18.146349	10-11
ATGGGGG	8375	0.0	16.713203	5
GTGGTAT	4025	0.0	16.651642	1
CTCGATA	765	5.5842975E-10	16.325829	94
>>END_MODULE