FastQCFastQC Report
Fri 27 May 2016
SRR2139157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940275
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136900.051.3248561
GTATCAA247800.036.8303261
TCAACGC299050.029.8434934
ATCAACG300150.029.6768863
CAACGCA305200.029.2843425
TATCAAC309350.029.0719722
AACGCAG314550.028.48586
GTGGTAT63400.025.132321
ACGCAGA356350.025.072227
CGCAGAG362900.024.6670368
TGGTATC62550.023.4139652
GCAGAGT412450.021.6099139
GAGTACT291400.020.57662612-13
GTACATG239100.019.758821
CAGAGTA408000.019.49259810-11
TACATGG238700.019.3601072
ACATGGG236450.018.7632873
AGAGTAC387100.018.74186110-11
GTACTTT320950.018.64098714-15
AGTACTT314800.017.6485912-13
Produced by FastQC (version 0.11.5)