##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8940275
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.680483765879686	33.0	33.0	33.0	33.0	33.0
2	32.69452606323631	33.0	33.0	33.0	33.0	33.0
3	32.67031394448157	33.0	33.0	33.0	33.0	33.0
4	36.611709035795876	37.0	37.0	37.0	37.0	37.0
5	36.63612964925576	37.0	37.0	37.0	37.0	37.0
6	36.60357091923906	37.0	37.0	37.0	37.0	37.0
7	36.512530542964285	37.0	37.0	37.0	37.0	37.0
8	36.54527886446446	37.0	37.0	37.0	37.0	37.0
9	36.59907049839071	37.0	37.0	37.0	37.0	37.0
10-11	36.580448308357404	37.0	37.0	37.0	37.0	37.0
12-13	36.56724737214459	37.0	37.0	37.0	37.0	37.0
14-15	38.69940152847647	40.0	37.0	40.0	37.0	40.0
16-17	38.638259561366965	40.0	37.0	40.0	37.0	40.0
18-19	38.660099381730426	40.0	37.0	40.0	37.0	40.0
20-21	38.66906910611688	40.0	37.0	40.0	37.0	40.0
22-23	38.65075112695921	40.0	37.0	40.0	37.0	40.0
24-25	38.67080513053067	40.0	37.0	40.0	37.0	40.0
26-27	38.59479996028381	40.0	37.0	40.0	37.0	40.0
28-29	38.48535438236826	40.0	37.0	40.0	37.0	40.0
30-31	38.35107750104248	40.0	37.0	40.0	37.0	40.0
32-33	38.25754944980003	40.0	37.0	40.0	37.0	40.0
34-35	38.224004400203626	40.0	37.0	40.0	37.0	40.0
36-37	38.199629312435405	40.0	37.0	40.0	37.0	40.0
38-39	38.162462873500345	40.0	37.0	40.0	37.0	40.0
40-41	38.128455239981136	40.0	37.0	40.0	37.0	40.0
42-43	38.08179409154904	40.0	37.0	40.0	37.0	40.0
44-45	37.98832973183367	40.0	37.0	40.0	33.0	40.0
46-47	37.9015698623675	40.0	37.0	40.0	33.0	40.0
48-49	37.83986119470774	40.0	37.0	40.0	33.0	40.0
50-51	37.798425791965556	40.0	37.0	40.0	33.0	40.0
52-53	37.734517244862445	40.0	37.0	40.0	33.0	40.0
54-55	37.640513184630564	40.0	37.0	40.0	33.0	40.0
56-57	37.48409207224336	40.0	37.0	40.0	33.0	40.0
58-59	37.283373445006575	38.5	37.0	40.0	33.0	40.0
60-61	37.120245523524595	37.0	37.0	40.0	33.0	40.0
62-63	36.98121413721845	37.0	37.0	40.0	33.0	40.0
64-65	36.81371407403462	37.0	37.0	40.0	33.0	40.0
66-67	36.606627792167714	37.0	37.0	40.0	33.0	40.0
68-69	36.411373592977654	37.0	37.0	40.0	33.0	40.0
70-71	36.195010390768594	37.0	37.0	38.5	33.0	40.0
72-73	35.90601750478088	37.0	37.0	37.0	33.0	40.0
74-75	35.567971463452295	37.0	33.0	37.0	33.0	40.0
76-77	33.40767898363639	35.0	33.0	37.0	27.0	37.0
78-79	35.0886537659682	37.0	33.0	37.0	33.0	37.0
80-81	35.30133049648854	37.0	33.0	37.0	33.0	37.0
82-83	35.16527638318999	37.0	33.0	37.0	33.0	37.0
84-85	34.990764216604504	37.0	33.0	37.0	33.0	37.0
86-87	34.86952272987526	37.0	33.0	37.0	33.0	37.0
88-89	34.76776973365996	37.0	33.0	37.0	33.0	37.0
90-91	34.67911958242963	37.0	33.0	37.0	33.0	37.0
92-93	34.65669662404456	37.0	33.0	37.0	33.0	37.0
94-95	34.61816733212234	37.0	33.0	37.0	33.0	37.0
96-97	34.614902895118476	37.0	33.0	37.0	33.0	37.0
98-99	34.62835066110204	37.0	33.0	37.0	33.0	37.0
100	34.58240610420522	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	44.0
21	209.0
22	889.0
23	2655.0
24	6619.0
25	13517.0
26	24281.0
27	39471.0
28	59418.0
29	84584.0
30	116124.0
31	156530.0
32	210349.0
33	286409.0
34	400330.0
35	595585.0
36	1070872.0
37	2645896.0
38	3135289.0
39	91198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.447349214649435	20.27750824219613	14.921755762546454	23.35338678060798
2	14.32053120757242	20.462428612047514	42.84070193241027	22.376338247969795
3	18.494330431670168	25.615766852809337	30.341046556174167	25.54885615934633
4	12.337774844733524	17.782450763539153	38.94050238946789	30.93927200225944
5	13.244659700065153	37.472314889642654	35.702313407585336	13.580712002706852
6	29.970890157181966	37.78560502892808	18.624136282161345	13.61936853172861
7	26.488804874570416	32.715089860211236	22.909272925049844	17.886832340168507
8	23.895696720738457	37.54838637513947	20.793924124257924	17.761992779864155
9	24.85810559518583	17.59168481953855	21.737989043961175	35.812220541314446
10-11	24.06545659948939	27.073177279222392	28.701175299417525	20.160190821870692
12-13	24.82430645991583	24.48962573075604	29.260471867444526	21.4255959418836
14-15	21.770644639007187	25.981795861984114	27.151933245901272	25.09562625310743
16-17	21.089463131726934	29.194359233916185	27.87209006434366	21.844087570013226
18-19	21.0245322431357	28.046726750575345	30.112188942733866	20.81655206355509
20-21	21.70425754544538	27.146170564791866	30.28757515831435	20.861996731448404
22-23	21.497471041383704	27.07715431035462	30.120546216355855	21.304828431905825
24-25	21.277634135674152	27.23475542931908	30.193025673317464	21.294584761689304
26-27	21.233806623828492	27.333979683344456	30.159648452747195	21.272565240079857
28-29	21.131440450844437	27.506906142311998	30.08998478341791	21.27166862342565
30-31	21.392312859914963	27.36193459071774	30.034935940125578	21.210816609241718
32-33	21.202523308517836	27.482371122687592	29.930172046877622	21.384933521916945
34-35	21.341251998609508	27.535555930539296	29.94652614238683	21.176665928464367
36-37	21.369744854753147	27.46575914977298	29.85547307858359	21.309022916890292
38-39	21.238746113573963	27.60889745126694	29.83623029547506	21.316126139684034
40-41	21.50755542893243	27.56630971656199	29.59633974177776	21.32979511272782
42-43	21.451713321378957	27.68686283657956	29.31980223747544	21.54162160456604
44-45	21.507664720032423	27.822079123261528	29.12674256331686	21.54351359338919
46-47	21.63160157237576	27.826803762994178	28.75121681831739	21.79037784631267
48-49	21.58841911597483	28.0181269853009	28.582496780337692	21.81095711838658
50-51	21.59225606761804	28.10829005520751	28.488248686775496	21.81120519039895
52-53	21.759930716108357	28.102847022545735	28.29593691330074	21.841285348045172
54-55	21.77447414221203	27.951499592364854	28.36270641261703	21.911319852806095
56-57	21.749987040900738	28.13304683342511	28.28222991793867	21.834736207735485
58-59	21.725281975822273	28.175668475040936	28.291095187525713	21.807954361611074
60-61	21.8403192719351	28.09929410943625	28.2616711960484	21.798715422580248
62-63	21.827918637306485	28.196044848018342	28.174301641559296	21.801734873115876
64-65	21.8316963027504	28.126484732839003	28.128575211640637	21.91324375276996
66-67	21.85381956714297	28.14937682379055	27.997895688245507	21.998907920820976
68-69	21.81002731826979	28.120557197129592	28.083984166553837	21.985431318046782
70-71	21.86001509050227	27.905693279862724	28.080814458495247	22.153477171139755
72-73	21.92230225240694	27.948481207875975	28.020239442359653	22.108977097357435
74-75	21.7732551150199	28.101527428177764	28.019460881102155	22.105756575700184
76-77	21.938954609607375	28.006275814619464	27.92609462654057	22.12867494923259
78-79	21.903403133930375	28.027315906552325	27.851984559884112	22.217296399633195
80-81	21.929626720813648	27.976981750750852	27.901326338532943	22.192065189902557
82-83	21.990161175759418	27.994002969037773	27.95797690320665	22.05785895199616
84-85	22.131392658996525	27.990455032238025	27.82409697035527	22.054055338410176
86-87	21.955984933977675	28.015961807261874	27.935335679090734	22.092717579669714
88-89	22.044487582036105	27.976350009436345	27.844130241427045	22.1350321671005
90-91	22.06644147303568	28.010234713860072	27.81043615367782	22.11288765942643
92-93	22.08334365763815	27.929446647317796	27.88853045111402	22.098679243930036
94-95	21.94681653298734	28.06098500621892	27.884716280905504	22.107482179888237
96-97	22.066429628256138	28.13265652500595	27.910832757069272	21.890081089668644
98-99	23.170396756698537	30.0528969747264	28.083227708299212	18.693478560275846
100	22.134656288673874	30.94148137907601	21.949421443002933	24.974440889247184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3312.0
1	2831.0
2	3274.0
3	4478.5
4	5865.0
5	9297.5
6	12568.0
7	15319.5
8	17468.0
9	17754.0
10	16425.5
11	14723.5
12	15680.0
13	20210.5
14	31245.5
15	46898.5
16	59818.0
17	67654.0
18	69861.0
19	66885.0
20	61210.0
21	54875.0
22	50745.0
23	51727.0
24	57722.0
25	68814.0
26	84277.5
27	100484.0
28	117043.5
29	136502.0
30	155158.5
31	173264.0
32	193217.0
33	213756.5
34	233561.0
35	251458.5
36	272909.0
37	294587.0
38	309736.0
39	319058.5
40	330033.5
41	342480.5
42	351690.0
43	360760.5
44	368031.0
45	373737.0
46	376314.0
47	374793.5
48	372361.5
49	366418.0
50	355032.5
51	337982.0
52	317475.5
53	295303.5
54	271155.5
55	244465.0
56	217923.0
57	192383.5
58	166649.0
59	141971.5
60	116311.0
61	92867.0
62	73355.0
63	55879.0
64	41100.0
65	30151.0
66	22775.5
67	17353.5
68	12977.5
69	9422.5
70	6722.5
71	4793.0
72	3429.5
73	2422.0
74	1644.5
75	1124.0
76	767.5
77	501.0
78	328.5
79	222.0
80	139.5
81	85.5
82	68.5
83	54.5
84	41.5
85	30.5
86	22.5
87	18.0
88	13.0
89	8.5
90	5.0
91	4.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.5859886860303515E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006274974763080554
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.0348368754809314E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0017572360389286356
36-37	0.03568974401809532
38-39	0.1172751141991714
40-41	0.15297490134579494
42-43	0.05427823499748031
44-45	0.001525831013693086
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.009358453484201291
64-65	0.07251953180214446
66-67	0.17322679328513169
68-69	0.19186894380060637
70-71	0.0755686095459196
72-73	0.005546409940718057
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	519.0
22-23	1247.0
24-25	2695.0
26-27	4286.0
28-29	7829.0
30-31	17467.0
32-33	21776.0
34-35	30217.0
36-37	43099.0
38-39	51544.0
40-41	55238.0
42-43	66860.0
44-45	77915.0
46-47	77687.0
48-49	66675.0
50-51	58977.0
52-53	54412.0
54-55	58269.0
56-57	59984.0
58-59	59010.0
60-61	59077.0
62-63	56280.0
64-65	61465.0
66-67	69183.0
68-69	63530.0
70-71	65116.0
72-73	61297.0
74-75	60197.0
76-77	55868.0
78-79	63293.0
80-81	63875.0
82-83	64444.0
84-85	68257.0
86-87	77736.0
88-89	84698.0
90-91	85492.0
92-93	92172.0
94-95	109462.0
96-97	298624.0
98-99	560634.0
100-101	6003730.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.31134379329971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4817858124255	73.7153647726409
2	7.886528731096084	13.140746129041636
3	1.999834288027184	4.998266456983845
4	0.7449272252232798	2.482435526462619
5	0.34710248788312015	1.4458787349770137
6	0.18566489046781753	0.9280794912065811
7	0.10518733286892658	0.6134308635940379
8	0.06332285853157635	0.42204099496789105
9	0.046827814679964136	0.35111593511022776
>10	0.13592614098589154	1.6947745821783538
>50	0.0023554032426519756	0.12407102005391633
>100	5.14098860489753E-4	0.07140518462288589
>500	2.291570756690422E-5	0.012390308160011273
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2370676517221225E-5	0.0	1.1185338258610612E-5	0.0
3	0.0	2.2370676517221225E-5	0.0	3.355601477583184E-5	0.0
4	0.0	4.474135303444245E-5	0.0	7.829736781027429E-5	0.0
5	0.0	4.474135303444245E-5	0.0	7.829736781027429E-5	0.0
6	1.3422405910332736E-4	4.474135303444245E-5	0.0	7.829736781027429E-5	0.0
7	1.3422405910332736E-4	4.474135303444245E-5	0.0	8.94827060688849E-5	0.0
8	1.3422405910332736E-4	6.711202955166368E-5	0.0	8.94827060688849E-5	1.1185338258610612E-5
9	1.3422405910332736E-4	1.5659473562054858E-4	0.0	1.4540939736193797E-4	3.355601477583184E-5
10-11	1.3422405910332736E-4	1.5659473562054858E-4	0.0	1.6218740474985388E-4	3.355601477583184E-5
12-13	1.3422405910332736E-4	1.6218740474985388E-4	0.0	1.9015075039638044E-4	3.355601477583184E-5
14-15	1.3422405910332736E-4	2.0692875778429635E-4	0.0	1.9574341952568574E-4	3.9148683905137144E-5
16-17	1.789654121377698E-4	2.2370676517221227E-4	0.0	2.0692875778429635E-4	4.474135303444245E-5
18-19	1.789654121377698E-4	2.572627799480441E-4	0.0	2.2370676517221227E-4	4.474135303444245E-5
20-21	1.789654121377698E-4	2.796334564652653E-4	0.0	2.460774416894335E-4	4.474135303444245E-5
22-23	1.789654121377698E-4	3.2996747862901307E-4	0.0	2.572627799480441E-4	4.474135303444245E-5
24-25	1.789654121377698E-4	3.635234934048449E-4	0.0	2.796334564652653E-4	4.474135303444245E-5
26-27	1.8455808126707513E-4	3.970795081806768E-4	0.0	3.46745486016929E-4	4.474135303444245E-5
28-29	1.9015075039638044E-4	4.082648464392874E-4	0.0	5.704522511891413E-4	4.474135303444245E-5
30-31	1.9015075039638044E-4	4.8096954512025637E-4	0.0	0.0012639432232229993	5.033402216374776E-5
32-33	1.9015075039638044E-4	4.92154883378867E-4	0.0	0.002790741895523348	7.829736781027429E-5
34-35	1.9015075039638044E-4	5.816375894477519E-4	0.0	0.004602766693418268	8.94827060688849E-5
36-37	1.9015075039638044E-4	6.543422881287208E-4	0.0	0.007046763102924687	8.94827060688849E-5
38-39	1.9015075039638044E-4	6.711202955166368E-4	0.0	0.011224486942515752	1.1185338258610613E-4
40-41	1.9015075039638044E-4	7.158616485510792E-4	0.0	0.015318320745167235	1.1185338258610613E-4
42-43	1.9015075039638044E-4	7.382323250683005E-4	0.0	0.0192835231578447	1.1185338258610613E-4
44-45	1.9015075039638044E-4	8.165296928785748E-4	0.0	0.023958994549943935	1.1185338258610613E-4
46-47	1.9015075039638044E-4	8.612710459130172E-4	0.0	0.028779875339405108	1.1185338258610613E-4
48-49	1.9015075039638044E-4	8.612710459130172E-4	0.0	0.03333230801065963	1.1185338258610613E-4
50-51	2.1252142691360166E-4	9.395684137232915E-4	0.0	0.0378399993288797	1.1185338258610613E-4
52-53	2.2370676517221227E-4	9.395684137232915E-4	0.0	0.042302949294065334	1.1185338258610613E-4
54-55	2.2370676517221227E-4	9.451610828525969E-4	0.0	0.046497451141044324	1.1744605171541144E-4
56-57	2.2370676517221227E-4	9.675317593698181E-4	0.0	0.050602470281954415	1.2303872084471675E-4
58-59	2.2370676517221227E-4	0.0010010877741456498	0.0	0.05493119618803672	1.2303872084471675E-4
60-61	2.572627799480441E-4	0.0010290511197921764	0.0	0.05921518074108459	1.3422405910332736E-4
62-63	3.1318947124109716E-4	0.001040236458050787	0.0	0.06354949931629619	1.4540939736193797E-4
64-65	3.1878214037040246E-4	0.0011073484876024508	0.0	0.06772722315588726	1.4540939736193797E-4
66-67	3.355601477583184E-4	0.0011129411567317561	0.0	0.07232998984930553	1.4540939736193797E-4
68-69	3.46745486016929E-4	0.0011353118332489772	0.0	0.0770502025944392	1.4540939736193797E-4
70-71	3.523381551462343E-4	0.0011409045023782825	0.0	0.08114962906622	1.4540939736193797E-4
72-73	4.3622819208581393E-4	0.0011464971715075879	0.0	0.08414170705039833	2.0133608865499105E-4
74-75	4.3622819208581393E-4	0.0011744605171541145	0.0	0.08654655477599962	2.0133608865499105E-4
76-77	4.3622819208581393E-4	0.0012136092010592515	0.0	0.08914155325199728	2.0133608865499105E-4
78-79	4.3622819208581393E-4	0.001219201870188557	0.0	0.0907466492921079	2.0133608865499105E-4
80-81	4.3622819208581393E-4	0.001219201870188557	0.0	0.09121084082984024	2.0692875778429635E-4
82-83	4.3622819208581393E-4	0.0012471652158350835	0.0	0.09154080830846925	2.1252142691360166E-4
84-85	4.4182086121511923E-4	0.0012751285614816098	0.0	0.09174773706625355	2.1252142691360166E-4
86-87	4.4741353034442454E-4	0.001297499237998831	0.0	0.09188196112535688	2.1252142691360166E-4
88	4.5859886860303515E-4	0.0013534259292918842	0.0	0.09194348048577924	2.1252142691360166E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13690	0.0	51.324856	1
GTATCAA	24780	0.0	36.830326	1
TCAACGC	29905	0.0	29.843493	4
ATCAACG	30015	0.0	29.676886	3
CAACGCA	30520	0.0	29.284342	5
TATCAAC	30935	0.0	29.071972	2
AACGCAG	31455	0.0	28.4858	6
GTGGTAT	6340	0.0	25.13232	1
ACGCAGA	35635	0.0	25.07222	7
CGCAGAG	36290	0.0	24.667036	8
TGGTATC	6255	0.0	23.413965	2
GCAGAGT	41245	0.0	21.609913	9
GAGTACT	29140	0.0	20.576626	12-13
GTACATG	23910	0.0	19.75882	1
CAGAGTA	40800	0.0	19.492598	10-11
TACATGG	23870	0.0	19.360107	2
ACATGGG	23645	0.0	18.763287	3
AGAGTAC	38710	0.0	18.741861	10-11
GTACTTT	32095	0.0	18.640987	14-15
AGTACTT	31480	0.0	17.64859	12-13
>>END_MODULE