FastQCFastQC Report
Fri 27 May 2016
SRR2139156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9350338
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147950.051.893231
GTATCAA279250.039.383631
ATCAACG359900.030.214273
TATCAAC362050.030.1880842
TCAACGC363200.029.9749974
CAACGCA364450.029.8838965
AACGCAG376050.028.9780656
ACGCAGA413050.026.3208437
CGCAGAG421350.025.8428828
GCAGAGT478700.022.5773899
TACATGG290850.021.9804842
GTACATG308300.021.4285091
ACATGGG293400.021.0621623
GAGTACT327250.020.4440312-13
AGAGTAC446600.019.90397310-11
GTGGTAT77200.019.8459381
CAGAGTA466000.019.7669210-11
CTTATAC54500.019.0284881
TGGTATC75700.018.8297732
CATGGGG211250.018.70724
Produced by FastQC (version 0.11.5)