##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9350338
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69903173553726	33.0	33.0	33.0	33.0	33.0
2	32.71103483104033	33.0	33.0	33.0	33.0	33.0
3	32.686468660277306	33.0	33.0	33.0	33.0	33.0
4	36.633800724636906	37.0	37.0	37.0	37.0	37.0
5	36.65923766606084	37.0	37.0	37.0	37.0	37.0
6	36.63125771496175	37.0	37.0	37.0	37.0	37.0
7	36.538692398071596	37.0	37.0	37.0	37.0	37.0
8	36.57366043879911	37.0	37.0	37.0	37.0	37.0
9	36.6243931502797	37.0	37.0	37.0	37.0	37.0
10-11	36.6062355179032	37.0	37.0	37.0	37.0	37.0
12-13	36.59315834358074	37.0	37.0	37.0	37.0	37.0
14-15	38.74433009801357	40.0	37.0	40.0	37.0	40.0
16-17	38.68759926111762	40.0	37.0	40.0	37.0	40.0
18-19	38.71143406794492	40.0	37.0	40.0	37.0	40.0
20-21	38.719480949357006	40.0	37.0	40.0	37.0	40.0
22-23	38.69955427251642	40.0	37.0	40.0	37.0	40.0
24-25	38.71894864493171	40.0	37.0	40.0	37.0	40.0
26-27	38.645892540846646	40.0	37.0	40.0	37.0	40.0
28-29	38.53828698184422	40.0	37.0	40.0	37.0	40.0
30-31	38.39592434921077	40.0	37.0	40.0	37.0	40.0
32-33	38.304013539230695	40.0	37.0	40.0	37.0	40.0
34-35	38.27096038193062	40.0	37.0	40.0	37.0	40.0
36-37	38.245628322065414	40.0	37.0	40.0	37.0	40.0
38-39	38.20101091483667	40.0	37.0	40.0	37.0	40.0
40-41	38.166361999808814	40.0	37.0	40.0	37.0	40.0
42-43	38.11722101404072	40.0	37.0	40.0	37.0	40.0
44-45	38.015315380735714	40.0	37.0	40.0	33.0	40.0
46-47	37.92930215727799	40.0	37.0	40.0	33.0	40.0
48-49	37.86347103790625	40.0	37.0	40.0	33.0	40.0
50-51	37.807268664880475	40.0	37.0	40.0	33.0	40.0
52-53	37.7354610450612	40.0	37.0	40.0	33.0	40.0
54-55	37.63337570565924	40.0	37.0	40.0	33.0	40.0
56-57	37.46319641902464	40.0	37.0	40.0	33.0	40.0
58-59	37.24902409902798	37.0	37.0	40.0	33.0	40.0
60-61	37.07236538874331	37.0	37.0	40.0	33.0	40.0
62-63	36.929266916526366	37.0	37.0	40.0	33.0	40.0
64-65	36.75433142132117	37.0	37.0	40.0	33.0	40.0
66-67	36.54693478360491	37.0	37.0	40.0	33.0	40.0
68-69	36.34550883519115	37.0	37.0	40.0	33.0	40.0
70-71	36.135799213799906	37.0	37.0	38.5	33.0	40.0
72-73	35.85608563290366	37.0	37.0	37.0	33.0	40.0
74-75	35.524668207231734	37.0	33.0	37.0	33.0	40.0
76-77	33.32904517881218	35.0	33.0	37.0	27.0	37.0
78-79	35.03931777552672	37.0	33.0	37.0	33.0	37.0
80-81	35.27680694273502	37.0	33.0	37.0	33.0	37.0
82-83	35.15374274978795	37.0	33.0	37.0	33.0	37.0
84-85	34.991584353477265	37.0	33.0	37.0	33.0	37.0
86-87	34.88232545210356	37.0	33.0	37.0	33.0	37.0
88-89	34.788806428221264	37.0	33.0	37.0	33.0	37.0
90-91	34.70558468050312	37.0	33.0	37.0	33.0	37.0
92-93	34.68759464731914	37.0	33.0	37.0	33.0	37.0
94-95	34.6545714545534	37.0	33.0	37.0	33.0	37.0
96-97	34.658907842691974	37.0	33.0	37.0	33.0	37.0
98-99	34.669672937413964	37.0	33.0	37.0	33.0	37.0
100	34.62534062189249	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	4.0
19	13.0
20	42.0
21	193.0
22	893.0
23	2664.0
24	6438.0
25	13508.0
26	24008.0
27	39137.0
28	59647.0
29	84580.0
30	116231.0
31	156019.0
32	210590.0
33	287401.0
34	403951.0
35	611021.0
36	1135403.0
37	2882726.0
38	3212062.0
39	103803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.611854031372985	20.23105474903688	14.689030492801436	23.4680607267887
2	14.459241111305044	20.457677097985265	42.51908814943343	22.563993641276262
3	17.94383261867111	26.244259833173945	30.65092406285206	25.160983485302886
4	12.216264267666046	17.723530422108805	39.13017903737811	30.930026272847034
5	13.035731970330913	37.769297751589306	35.67076398735532	13.524206290724464
6	29.67465988930026	38.04777966315228	18.630449508884066	13.647110938663392
7	26.282686251555827	32.99730982986925	23.15104544883832	17.5689584697366
8	24.43512737186613	36.54006946059062	21.078564218748028	17.94623894879522
9	25.425604935350997	16.972851676591798	21.619795990262595	35.98174739779461
10-11	24.226466465704235	26.806913290193357	28.73252817170887	20.234092072393533
12-13	25.03258785163395	24.317476024566066	29.00483773509311	21.64509838870688
14-15	22.10179460892216	25.61806321867723	27.161285506470463	25.11885666593015
16-17	21.32502055005926	28.69528887618822	27.772477315793292	22.20721325795923
18-19	21.23892205821864	27.536453762420138	30.0802602002195	21.144363979141716
20-21	22.156571829177548	26.739305682522176	30.040050298833144	21.064072189467137
22-23	21.808551458939576	26.69460283084158	29.916089361997745	21.5807563482211
24-25	21.43054052857093	26.917282181703506	30.11812261834031	21.534054671385253
26-27	21.51758240047264	27.08479992226822	29.926343683498548	21.471273993760594
28-29	21.38931528698567	27.24168270299464	29.858504122189323	21.510497887830365
30-31	21.68752070828069	27.014565603592004	29.83961867119965	21.458295016927654
32-33	21.40319231567382	27.17358704654314	29.77045726061997	21.65276337716307
34-35	21.47767769589129	27.29552514334534	29.699962795845607	21.52683436491776
36-37	21.64876969058479	27.143533178634023	29.689453634867945	21.518243495913243
38-39	21.5316214834916	27.27007710563335	29.641517173823868	21.556784237051183
40-41	21.793427958001583	27.272610055661666	29.464815040336255	21.469146946000496
42-43	21.831054868326763	27.33459100103485	29.130185090780536	21.704169039857852
44-45	21.852096395054836	27.731841490602484	28.66097093248753	21.755091181855153
46-47	22.036680477937594	27.530196612927604	28.286505496986415	22.146617412148387
48-49	22.10269534950509	27.552699835077625	28.204304005588064	22.140300809829217
50-51	21.898935967822926	27.778626161292475	28.131773619580013	22.190664251304582
52-53	22.137256310352843	27.742406022462347	27.892700660439512	22.2276370067453
54-55	22.156259148849596	27.674683669780205	27.849908841829063	22.319148339541137
56-57	22.22664342502682	27.935990310221946	27.68141622551847	22.155950039232767
58-59	22.12053137225948	27.97318338659524	27.87251887547444	22.033766365670836
60-61	22.20677539785444	27.886866752594585	27.74576157628838	22.160596273262595
62-63	22.221672829603826	27.985067508631982	27.600725111093034	22.19253455067116
64-65	22.167234691735377	27.920798316031913	27.62886189284499	22.283105099387722
66-67	22.272376660862815	27.87544854005748	27.449405068762832	22.40276973031688
68-69	22.291947788556715	27.762998623710384	27.615965301946005	22.329088285786895
70-71	22.232026566105144	27.42493277204921	27.62775623803521	22.71528442381043
72-73	22.400368743567295	27.50236300264575	27.625709095311564	22.47155915847539
74-75	22.25551175577936	27.581014626692667	27.590415404405938	22.573058213122042
76-77	22.3356917035686	27.632874707191245	27.418868767571773	22.612564821668375
78-79	22.35786427028398	27.590495291091123	27.43862143973236	22.613018998892542
80-81	22.320061129444497	27.478643925578407	27.568432486809375	22.632862458167722
82-83	22.56167815151951	27.356201565542122	27.62897690027054	22.453143382667832
84-85	22.75889225175236	27.31362410516283	27.439242887061223	22.48824075602359
86-87	22.393492759154633	27.429234706132217	27.534767695916152	22.642504838797002
88-89	22.58992124655675	27.27009041529512	27.500594418071827	22.639393920076305
90-91	22.67262917082943	27.23685239829981	27.54507534396006	22.545443086910698
92-93	22.680422659980735	27.316670524972192	27.450453808615155	22.552453006431914
94-95	22.406685217811	27.315589958504866	27.62934680343687	22.648378020247268
96-97	22.57589145185374	27.349297428047155	27.685779260133543	22.389031859965563
98-99	23.749025182067385	29.276275232941952	27.72552894050701	19.249170644483655
100	22.77065379969835	30.153416133315282	21.449289540054632	25.62664052693173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1475.0
1	1605.5
2	3124.0
3	4857.0
4	6947.5
5	12419.0
6	17058.5
7	19549.0
8	20529.0
9	19214.0
10	16975.0
11	15212.5
12	17008.5
13	23309.0
14	37505.5
15	57407.0
16	74004.0
17	83565.5
18	84889.0
19	79101.0
20	69634.5
21	59583.5
22	52588.5
23	51552.5
24	56337.5
25	67945.0
26	85450.0
27	102455.5
28	116199.5
29	132465.5
30	147975.5
31	162127.5
32	183158.0
33	205476.0
34	226645.0
35	247891.0
36	273685.0
37	297452.5
38	307177.5
39	314019.5
40	328984.5
41	340714.5
42	348496.0
43	359585.0
44	371020.0
45	382528.5
46	390242.5
47	397033.0
48	399326.0
49	390776.0
50	378921.0
51	366019.5
52	351451.5
53	338183.0
54	319797.5
55	292106.0
56	260958.5
57	227607.5
58	194615.0
59	164421.5
60	131626.0
61	100873.5
62	76837.5
63	57515.0
64	42598.5
65	30684.0
66	22106.0
67	16519.5
68	12066.0
69	8639.0
70	6399.0
71	4653.0
72	3301.0
73	2199.5
74	1502.5
75	1032.5
76	702.0
77	462.5
78	306.5
79	218.5
80	157.5
81	115.0
82	90.0
83	68.5
84	50.0
85	37.0
86	28.5
87	24.5
88	17.0
89	9.0
90	8.0
91	7.0
92	3.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.6362321875423113E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.006539870537300363
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.1394950791613178E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0018636741733056468
36-37	0.037278523975841873
38-39	0.11723604343091387
40-41	0.15241772756733177
42-43	0.055398394522895605
44-45	0.0017496067952513235
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.009359268629312231
64-65	0.07237033458908877
66-67	0.17319792075515597
68-69	0.19203468024510817
70-71	0.07594941234126791
72-73	0.005564456542123465
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	109.0
20-21	562.0
22-23	1185.0
24-25	2583.0
26-27	4749.0
28-29	9021.0
30-31	19191.0
32-33	23287.0
34-35	30010.0
36-37	39054.0
38-39	41163.0
40-41	58843.0
42-43	70437.0
44-45	79799.0
46-47	89283.0
48-49	73827.0
50-51	63424.0
52-53	62121.0
54-55	63747.0
56-57	64313.0
58-59	66801.0
60-61	60064.0
62-63	53666.0
64-65	70847.0
66-67	89339.0
68-69	80254.0
70-71	80345.0
72-73	77037.0
74-75	75649.0
76-77	70159.0
78-79	78563.0
80-81	83909.0
82-83	80949.0
84-85	91261.0
86-87	96664.0
88-89	104421.0
90-91	103440.0
92-93	108890.0
94-95	122556.0
96-97	309835.0
98-99	576999.0
100-101	6071982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.21081104676232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60411449748305	65.23958994793088
2	8.450698033570683	12.880691020994023
3	2.8087100422055387	6.421622109350104
4	1.273433867152103	3.88197711320307
5	0.6377213104138256	2.430062914422086
6	0.377323156870432	1.725366228709221
7	0.22698739680937804	1.210922552576518
8	0.1633894931961307	0.9961636634397258
9	0.10231053264876512	0.7017451804609781
>10	0.3498739017540811	3.957256843693501
>50	0.003718215273313547	0.18550518887168035
>100	0.0014732867951338681	0.1762079174038846
>500	1.8469938400533193E-4	0.11342981468469722
>1k	6.156644353670714E-5	0.0794595042595925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.347400275797517E-5
2	0.0	0.0	0.0	0.0	5.347400275797517E-5
3	0.0	0.0	0.0	0.0	5.347400275797517E-5
4	0.0	0.0	0.0	0.0	5.347400275797517E-5
5	0.0	0.0	0.0	0.0	6.41688033095702E-5
6	0.0	0.0	0.0	0.0	6.41688033095702E-5
7	0.0	0.0	0.0	0.0	6.41688033095702E-5
8	1.0694800551595033E-5	0.0	0.0	0.0	6.41688033095702E-5
9	1.0694800551595033E-5	0.0	0.0	1.0694800551595033E-5	6.41688033095702E-5
10-11	5.882140303377268E-5	0.0	0.0	1.0694800551595033E-5	9.090580468855778E-5
12-13	9.62532049643553E-5	0.0	0.0	1.0694800551595033E-5	3.6362321875423113E-4
14-15	9.62532049643553E-5	0.0	0.0	1.0694800551595033E-5	4.0640242096061125E-4
16-17	1.2299020634334287E-4	0.0	0.0	2.6737001378987583E-5	4.6522382399438397E-4
18-19	1.3903240717073542E-4	0.0	0.0	3.20844016547851E-5	4.812660248217765E-4
20-21	1.3903240717073542E-4	0.0	0.0	3.20844016547851E-5	5.347400275797517E-4
22-23	3.3688621737524356E-4	0.0	0.0	5.347400275797517E-5	5.561296286829417E-4
24-25	4.117498212364088E-4	0.0	0.0	1.283376066191404E-4	5.668244292345368E-4
26-27	4.812660248217765E-4	0.0	0.0	2.2993821185929322E-4	6.149510317167144E-4
28-29	5.133504264765616E-4	0.0	0.0	5.293926273039541E-4	6.30993232544107E-4
30-31	5.668244292345368E-4	0.0	0.0	0.0014758824761201147	6.30993232544107E-4
32-33	5.775192297861318E-4	0.0	0.0	0.003240524567133295	6.844672353020821E-4
34-35	5.828666300619293E-4	0.0	0.0	0.0052939262730395415	6.951620358536772E-4
36-37	6.470354333714995E-4	0.0	0.0	0.008106658818109035	7.005094361294747E-4
38-39	6.52382833647297E-4	0.0	0.0	0.011935397415580057	7.165516369568672E-4
40-41	6.791198350262846E-4	0.0	0.0	0.015988726824634578	7.165516369568672E-4
42-43	7.112042366810697E-4	0.0	0.0	0.020587491061820437	7.272464375084623E-4
44-45	7.165516369568672E-4	0.0	0.0	0.025458972713071976	7.593308391632474E-4
46-47	7.272464375084623E-4	0.0	0.0	0.030747551585835718	7.967626410938299E-4
48-49	7.379412380600573E-4	0.0	0.0	0.03581153964701597	8.716262449549952E-4
50-51	7.967626410938299E-4	0.0	0.0	0.04028731367785849	9.197528474371729E-4
52-53	8.234996424728176E-4	0.0	0.0	0.04460801310070288	9.678794499193505E-4
54-55	8.555840441276027E-4	0.0	0.0	0.04842605689762231	0.0010106586521257306
56-57	8.930158460581852E-4	0.0	0.0	0.05235104870005769	0.0010641326548837056
58-59	0.001016006052401528	0.0	0.0	0.05607818669228856	0.0010801748557110983
60-61	0.0010641326548837058	0.0	0.0	0.05958073387293593	0.0010801748557110983
62-63	0.0010748274554353008	0.0	0.0	0.06255388842627935	0.001171080660399656
64-65	0.001096217056538491	0.0	0.0	0.06558586438265654	0.0012192072628818339
66-67	0.0011122592573658834	0.0	0.0	0.07053755703804504	0.0012405968639850238
68-69	0.0011122592573658834	0.0	0.0	0.0753823016879176	0.001251291664536619
70-71	0.0011122592573658834	0.0	0.0	0.07995967632400028	0.001294070866742999
72-73	0.0011176066576416809	0.0	0.0	0.08404509013470957	0.0013528922697767717
74-75	0.0011229540579174785	0.0	0.0	0.08694338108419182	0.0014010188722589495
76-77	0.0011229540579174785	0.0	0.0	0.08970798702677914	0.001417061073086342
78-79	0.0011229540579174785	0.0	0.0	0.09152610312055029	0.0014758824761201147
80-81	0.0011550384595722636	0.0	0.0	0.09218383335447339	0.0015186616783264947
82-83	0.0011764280606754536	0.0	0.0	0.09266509937929517	0.001604220082739255
84-85	0.0011764280606754536	0.0	0.0	0.09288969019087866	0.0016309570841182425
86-87	0.0011764280606754536	0.0	0.0	0.09306080699970418	0.001716515488531003
88	0.0011764280606754536	0.0	0.0	0.09308754400108317	0.0017967264926679657
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14795	0.0	51.89323	1
GTATCAA	27925	0.0	39.38363	1
ATCAACG	35990	0.0	30.21427	3
TATCAAC	36205	0.0	30.188084	2
TCAACGC	36320	0.0	29.974997	4
CAACGCA	36445	0.0	29.883896	5
AACGCAG	37605	0.0	28.978065	6
ACGCAGA	41305	0.0	26.320843	7
CGCAGAG	42135	0.0	25.842882	8
GCAGAGT	47870	0.0	22.577389	9
TACATGG	29085	0.0	21.980484	2
GTACATG	30830	0.0	21.428509	1
ACATGGG	29340	0.0	21.062162	3
GAGTACT	32725	0.0	20.44403	12-13
AGAGTAC	44660	0.0	19.903973	10-11
GTGGTAT	7720	0.0	19.845938	1
CAGAGTA	46600	0.0	19.76692	10-11
CTTATAC	5450	0.0	19.028488	1
TGGTATC	7570	0.0	18.829773	2
CATGGGG	21125	0.0	18.7072	4
>>END_MODULE