FastQCFastQC Report
Fri 27 May 2016
SRR2139155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409735
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154550.050.8573341
GTATCAA289850.039.0986021
CTTATAC89200.032.8842661
ATCAACG363400.030.535833
TCAACGC372250.029.8664684
CAACGCA373950.029.708155
TATCAAC379900.029.4869232
AACGCAG389300.028.571996
TATACAC131400.026.524913
ACGCAGA431850.025.6886087
CGCAGAG440400.025.2281678
GTGGTAT71750.022.8492261
GCAGAGT502800.022.0385449
TTATACA145000.021.9734152
TGGTATC72500.020.9270612
TACATGG316050.020.4289822
GTACATG324250.020.3282761
ACATGGG310350.020.247423
ACACATC150550.020.2415166
AGAGTAC474250.019.52142110-11
Produced by FastQC (version 0.11.5)