##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10409735
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67157953588636	33.0	33.0	33.0	33.0	33.0
2	32.690155224892855	33.0	33.0	33.0	33.0	33.0
3	32.66354590198502	33.0	33.0	33.0	33.0	33.0
4	36.60634732776579	37.0	37.0	37.0	37.0	37.0
5	36.633284997168516	37.0	37.0	37.0	37.0	37.0
6	36.603103345089956	37.0	37.0	37.0	37.0	37.0
7	36.51294177997807	37.0	37.0	37.0	37.0	37.0
8	36.545076027391666	37.0	37.0	37.0	37.0	37.0
9	36.59927241183373	37.0	37.0	37.0	37.0	37.0
10-11	36.58126911972303	37.0	37.0	37.0	37.0	37.0
12-13	36.56790292932529	37.0	37.0	37.0	37.0	37.0
14-15	38.69512350698649	40.0	37.0	40.0	37.0	40.0
16-17	38.63438901182403	40.0	37.0	40.0	37.0	40.0
18-19	38.66238866791518	40.0	37.0	40.0	37.0	40.0
20-21	38.67534481706869	40.0	37.0	40.0	37.0	40.0
22-23	38.65545174648455	40.0	37.0	40.0	37.0	40.0
24-25	38.67485616422164	40.0	37.0	40.0	37.0	40.0
26-27	38.601610081528804	40.0	37.0	40.0	37.0	40.0
28-29	38.493831349504205	40.0	37.0	40.0	37.0	40.0
30-31	38.35547770860403	40.0	37.0	40.0	37.0	40.0
32-33	38.26088614218733	40.0	37.0	40.0	37.0	40.0
34-35	38.22343742824141	40.0	37.0	40.0	37.0	40.0
36-37	38.21112286887087	40.0	37.0	40.0	37.0	40.0
38-39	38.16985911386651	40.0	37.0	40.0	37.0	40.0
40-41	38.14856264168383	40.0	37.0	40.0	37.0	40.0
42-43	38.10605804797522	40.0	37.0	40.0	37.0	40.0
44-45	38.0128245434958	40.0	37.0	40.0	33.0	40.0
46-47	37.93608502377337	40.0	37.0	40.0	33.0	40.0
48-49	37.881599922478756	40.0	37.0	40.0	33.0	40.0
50-51	37.83074124350379	40.0	37.0	40.0	33.0	40.0
52-53	37.77244227311785	40.0	37.0	40.0	33.0	40.0
54-55	37.67172913611897	40.0	37.0	40.0	33.0	40.0
56-57	37.51039389166034	40.0	37.0	40.0	33.0	40.0
58-59	37.31125134693515	38.5	37.0	40.0	33.0	40.0
60-61	37.15038337031532	37.0	37.0	40.0	33.0	40.0
62-63	37.02225864724377	37.0	37.0	40.0	33.0	40.0
64-65	36.86217080900939	37.0	37.0	40.0	33.0	40.0
66-67	36.66692266545574	37.0	37.0	40.0	33.0	40.0
68-69	36.47685260328073	37.0	37.0	40.0	33.0	40.0
70-71	36.271463637994216	37.0	37.0	40.0	33.0	40.0
72-73	35.99072109835942	37.0	37.0	37.0	33.0	40.0
74-75	35.65811109825793	37.0	35.0	37.0	33.0	40.0
76-77	33.478386456804515	35.0	33.0	37.0	27.0	37.0
78-79	35.16477339869213	37.0	33.0	37.0	33.0	37.0
80-81	35.37722949278215	37.0	33.0	37.0	33.0	37.0
82-83	35.242693834275926	37.0	33.0	37.0	33.0	37.0
84-85	35.07570014580586	37.0	33.0	37.0	33.0	37.0
86-87	34.95934538663351	37.0	33.0	37.0	33.0	37.0
88-89	34.865022984851294	37.0	33.0	37.0	33.0	37.0
90-91	34.778644345148706	37.0	33.0	37.0	33.0	37.0
92-93	34.75807533333479	37.0	33.0	37.0	33.0	37.0
94-95	34.72043739828852	37.0	33.0	37.0	33.0	37.0
96-97	34.72076386699519	37.0	33.0	37.0	33.0	37.0
98-99	34.732946388775346	37.0	33.0	37.0	33.0	37.0
100	34.6892013286657	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	4.0
20	43.0
21	262.0
22	1070.0
23	3222.0
24	7574.0
25	15279.0
26	26962.0
27	44202.0
28	66055.0
29	94355.0
30	129749.0
31	174753.0
32	234331.0
33	319111.0
34	448634.0
35	668500.0
36	1218244.0
37	3086506.0
38	3746343.0
39	124533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78556666289456	20.90452071109598	14.854375721800384	23.45553690420908
2	13.995899206239724	20.69760565442282	43.36016858135668	21.946326557980775
3	17.682699673209708	26.796102570920638	30.876946519478786	24.64425123639086
4	11.801723271699354	18.338067043788055	39.73174530684454	30.128464377668056
5	12.735464710241395	38.012863729274926	36.05778015077043	13.193891409713254
6	28.64095278515282	38.784699013442605	19.108221209110628	13.466126992293944
7	25.583679659826082	33.185257183324765	23.571783870750203	17.659279286098954
8	23.755890400273437	37.21060499720742	21.481644007426127	17.551860595093018
9	24.910319514408343	17.17284034347083	21.888657289417772	36.028182852703054
10-11	23.68206412949341	27.25717452255867	29.08246603215481	19.978295315793115
12-13	24.656195843405897	24.66879065152049	29.324205443896854	21.35080806117676
14-15	21.721191749838013	25.99622853031321	27.371878342724383	24.910701377124393
16-17	20.89593058805051	29.221070469132982	28.013854339231496	21.86914460358501
18-19	20.747190010120335	28.000025937259693	30.3326357491329	20.92014830348707
20-21	21.654228818680952	27.195825425085623	30.20733674682076	20.94260900941266
22-23	21.35235932320403	27.286801492912787	30.10596487321112	21.25487431067206
24-25	21.10960183071287	27.360756391782203	30.405602678596093	21.12403909890884
26-27	21.058287215072195	27.536110271665205	30.261730263345076	21.14387224991753
28-29	20.774933852446956	27.664022367538983	30.38040493555483	21.180638844459235
30-31	21.251106197407015	27.41056167933299	30.038140786081684	21.300191337178312
32-33	21.235242586924503	27.592191835943442	30.141219735042355	21.031345842089692
34-35	21.187522484812007	27.87448015598831	30.081214051536765	20.856783307662923
36-37	21.148646610909026	27.595916338246585	29.953327217309837	21.30210983353455
38-39	21.11341238620602	27.62820958941969	30.059556329028652	21.198821695345647
40-41	21.340937125927834	27.57477348328306	29.89012201308416	21.19416737770495
42-43	21.397063941197153	27.654521690820843	29.697863738347852	21.25055062963415
44-45	21.293745037412215	28.00683121814349	29.31161734749974	21.387806396944555
46-47	21.584633495775606	27.775479844671324	28.745850732764154	21.894035926788916
48-49	21.64579093462324	27.86592237472103	28.709047352190314	21.77923933846542
50-51	21.409330035922434	28.057594735716773	28.678933680063718	21.854141548297076
52-53	21.50395022454544	28.160335903476092	28.631356269457864	21.70435760252061
54-55	21.570274499434454	28.161334641228585	28.38095246096188	21.887438398375078
56-57	21.752162382725803	28.299786950761995	28.176290361293372	21.77176030521883
58-59	21.489999858231798	28.50578186902383	28.48503021401381	21.519188058730553
60-61	21.750365325319517	28.64466453287264	28.02980218559515	21.575167956212702
62-63	21.705502525291926	28.68775166582412	27.899301045109798	21.707444763774152
64-65	21.623694617480858	28.651686787267305	27.94335915411373	21.781259441138108
66-67	21.728336296153987	28.482406404626765	27.874556058317506	21.91470124090174
68-69	21.765134993178194	28.288026996377507	28.090926252685477	21.85591175775882
70-71	21.818168356346987	27.895310727754296	28.10516312883175	22.18135778706696
72-73	21.89641684444782	27.980428309019366	28.14104907564335	21.98210577088946
74-75	21.744084456593722	28.17998192075945	28.06027759682226	22.01565602582457
76-77	21.78935957186637	28.084173725192723	27.95902233923824	22.167444363702664
78-79	21.884674649062045	28.004093780773815	27.947062869629598	22.164168700534542
80-81	21.79375176428944	27.94644028001607	28.110668182150846	22.14913977354365
82-83	22.019880216307477	27.852615999881646	28.13244138150083	21.99506240231005
84-85	22.24978711960131	27.842557254764255	27.910539719075445	21.99711590655899
86-87	21.782142842793064	27.95597077039716	28.078283114721213	22.18360327208856
88-89	22.08037576934968	27.740900705945243	28.03251823165317	22.14620529305191
90-91	22.154042275067734	27.743260122480002	28.054843193778883	22.047854408673384
92-93	22.133615130683005	27.72841728889614	28.02097916333295	22.116988417087907
94-95	21.97959880956416	27.742041351720193	28.088963919608023	22.18939591910763
96-97	22.006539724980673	27.907486276771454	28.152106785199987	21.933867213047886
98-99	23.137434390315057	29.756235112876112	28.35838721398836	18.74794328282047
100	22.22607650601587	30.541208211748888	22.10014461151399	25.13257067072125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1517.0
1	1597.5
2	3218.5
3	5121.0
4	7357.0
5	13129.0
6	17979.5
7	20521.0
8	21483.5
9	19987.0
10	17726.0
11	15914.5
12	17563.5
13	24205.0
14	39645.5
15	61691.0
16	80350.0
17	91087.0
18	93170.0
19	87985.0
20	79030.0
21	69437.5
22	63335.0
23	65176.0
24	74219.0
25	89583.0
26	111060.5
27	133048.0
28	150212.0
29	168997.0
30	189338.5
31	209706.0
32	233898.5
33	257735.0
34	282981.5
35	307766.0
36	333252.0
37	359077.0
38	373105.0
39	381315.0
40	391231.0
41	399240.5
42	406793.0
43	416674.0
44	425671.0
45	434472.5
46	443906.5
47	452510.0
48	461158.5
49	453056.0
50	433900.0
51	417772.5
52	393455.0
53	360141.5
54	324664.0
55	290014.5
56	254852.0
57	220629.5
58	187444.0
59	156948.0
60	125732.0
61	94879.0
62	71164.5
63	51989.5
64	36651.0
65	24937.5
66	17283.5
67	12879.5
68	9485.5
69	6814.0
70	5118.0
71	3738.0
72	2669.5
73	1860.0
74	1317.5
75	898.5
76	606.0
77	423.0
78	272.5
79	176.0
80	125.5
81	92.5
82	63.5
83	34.5
84	20.5
85	14.5
86	11.5
87	12.0
88	9.5
89	6.5
90	3.5
91	1.0
92	2.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.606392477810433E-6
2	4.130748765458487E-4
3	9.606392477810433E-6
4	9.606392477810433E-6
5	9.606392477810433E-6
6	9.606392477810433E-6
7	9.606392477810433E-6
8	9.606392477810433E-6
9	9.606392477810433E-6
10-11	4.803196238905217E-6
12-13	0.006570772454822337
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.60882982277138E-6
26-27	0.0
28-29	0.0
30-31	9.63114589303139E-6
32-33	9.65420100143027E-6
34-35	0.0016752871325940379
36-37	0.03672642624642649
38-39	0.11733119448292433
40-41	0.15341170851243352
42-43	0.054651792560921335
44-45	0.0015241307754341204
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.00892477127000477
64-65	0.07311833384090057
66-67	0.175584969351611
68-69	0.1946717818882729
70-71	0.0774599289560819
72-73	0.005292068453508776
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	130.0
20-21	571.0
22-23	1367.0
24-25	2914.0
26-27	5350.0
28-29	10776.0
30-31	23654.0
32-33	29430.0
34-35	39825.0
36-37	57192.0
38-39	70593.0
40-41	76461.0
42-43	91985.0
44-45	107633.0
46-47	103934.0
48-49	90793.0
50-51	79739.0
52-53	74289.0
54-55	85306.0
56-57	89796.0
58-59	87025.0
60-61	92832.0
62-63	91106.0
64-65	94358.0
66-67	105221.0
68-69	89837.0
70-71	85716.0
72-73	84287.0
74-75	83736.0
76-77	79580.0
78-79	83627.0
80-81	86974.0
82-83	94043.0
84-85	96401.0
86-87	102060.0
88-89	111014.0
90-91	110711.0
92-93	116651.0
94-95	132125.0
96-97	329382.0
98-99	621241.0
100-101	6590070.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.13107999122789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.15589209873373	67.31452897281764
2	8.481741361123389	13.253752255016751
3	2.590420172711805	6.071769771751092
4	1.1508765125559979	3.596768994501523
5	0.5518167621455858	2.155701979184857
6	0.3573027622996446	1.674987042139213
7	0.20933998229900938	1.1449171231656292
8	0.1387901586481359	0.8675059989866172
9	0.09087582939185257	0.6390204025935611
>10	0.26993992048063975	3.001444822895711
>50	0.0021975563173625558	0.1129688335152619
>100	6.836349049002295E-4	0.09131163845816254
>500	1.2324838792663828E-4	0.07532216497402049
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.606392477810435E-6	0.0
2	0.0	0.0	0.0	9.606392477810435E-6	0.0
3	0.0	0.0	0.0	3.842556991124174E-5	0.0
4	0.0	0.0	0.0	4.803196238905217E-5	0.0
5	0.0	0.0	0.0	5.7638354866862606E-5	0.0
6	0.0	0.0	0.0	6.724474734467304E-5	0.0
7	0.0	0.0	0.0	6.724474734467304E-5	0.0
8	0.0	0.0	0.0	6.724474734467304E-5	0.0
9	0.0	0.0	0.0	1.5370227964496696E-4	0.0
10-11	0.0	0.0	0.0	2.545694006619765E-4	0.0
12-13	0.0	0.0	0.0	3.3622373672336517E-4	0.0
14-15	0.0	0.0	0.0	3.3622373672336517E-4	0.0
16-17	0.0	0.0	0.0	3.698461103957017E-4	0.0
18-19	0.0	0.0	0.0	4.3709085774037477E-4	0.0
20-21	0.0	0.0	0.0	5.091388013239531E-4	0.0
22-23	0.0	0.0	0.0	5.331547825184791E-4	0.0
24-25	0.0	0.0	0.0	5.427611749962895E-4	0.0
26-27	0.0	0.0	0.0	5.715803524297208E-4	0.0
28-29	9.606392477810435E-6	0.0	0.0	7.877241831804557E-4	0.0
30-31	9.606392477810435E-6	0.0	0.0	0.0013641077318490816	0.0
32-33	9.606392477810435E-6	0.0	0.0	0.0028002634072817416	0.0
34-35	9.606392477810435E-6	0.0	0.0	0.004543823642004336	0.0
36-37	9.606392477810435E-6	0.0	0.0	0.007031879293757238	0.0
38-39	9.606392477810435E-6	0.0	0.0	0.011191447236649155	0.0
40-41	9.606392477810435E-6	0.0	0.0	0.015749680467370207	0.0
42-43	9.606392477810435E-6	0.0	0.0	0.020221456165790963	0.0
44-45	9.606392477810435E-6	0.0	0.0	0.02543772728124203	4.8031962389052175E-6
46-47	9.606392477810435E-6	0.0	0.0	0.03142250979491793	9.606392477810435E-6
48-49	9.606392477810435E-6	0.0	0.0	0.03738807952363821	9.606392477810435E-6
50-51	9.606392477810435E-6	0.0	0.0	0.043161521402802286	9.606392477810435E-6
52-53	9.606392477810435E-6	0.0	0.0	0.04869000027378219	9.606392477810435E-6
54-55	9.606392477810435E-6	0.0	0.0	0.05393028737042778	9.606392477810435E-6
56-57	9.606392477810435E-6	0.0	0.0	0.05912254250468432	9.606392477810435E-6
58-59	9.606392477810435E-6	0.0	0.0	0.06428597846150742	9.606392477810435E-6
60-61	9.606392477810435E-6	0.0	0.0	0.06928130254996885	9.606392477810435E-6
62-63	9.606392477810435E-6	0.0	0.0	0.07432465860081933	9.606392477810435E-6
64-65	9.606392477810435E-6	0.0	0.0	0.07934399867047529	9.606392477810435E-6
66-67	9.606392477810435E-6	0.0	0.0	0.08475720083172146	9.606392477810435E-6
68-69	9.606392477810435E-6	0.0	0.0	0.08987740802239443	9.606392477810435E-6
70-71	9.606392477810435E-6	0.0	0.0	0.09393130564803043	9.606392477810435E-6
72-73	9.606392477810435E-6	0.0	0.0	0.09746165488362576	9.606392477810435E-6
74-75	9.606392477810435E-6	0.0	0.0	0.10035798221568559	9.606392477810435E-6
76-77	9.606392477810435E-6	0.0	0.0	0.10326391594022327	9.606392477810435E-6
78-79	9.606392477810435E-6	0.0	0.0	0.1051467688658741	9.606392477810435E-6
80-81	9.606392477810435E-6	0.0	0.0	0.10573756200325946	9.606392477810435E-6
82-83	9.606392477810435E-6	0.0	0.0	0.10626591358953902	9.606392477810435E-6
84-85	9.606392477810435E-6	0.0	0.0	0.10653489257891771	9.606392477810435E-6
86-87	9.606392477810435E-6	0.0	0.0	0.10665977568112925	9.606392477810435E-6
88	9.606392477810435E-6	0.0	0.0	0.10667898846608487	9.606392477810435E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15455	0.0	50.857334	1
GTATCAA	28985	0.0	39.098602	1
CTTATAC	8920	0.0	32.884266	1
ATCAACG	36340	0.0	30.53583	3
TCAACGC	37225	0.0	29.866468	4
CAACGCA	37395	0.0	29.70815	5
TATCAAC	37990	0.0	29.486923	2
AACGCAG	38930	0.0	28.57199	6
TATACAC	13140	0.0	26.52491	3
ACGCAGA	43185	0.0	25.688608	7
CGCAGAG	44040	0.0	25.228167	8
GTGGTAT	7175	0.0	22.849226	1
GCAGAGT	50280	0.0	22.038544	9
TTATACA	14500	0.0	21.973415	2
TGGTATC	7250	0.0	20.927061	2
TACATGG	31605	0.0	20.428982	2
GTACATG	32425	0.0	20.328276	1
ACATGGG	31035	0.0	20.24742	3
ACACATC	15055	0.0	20.241516	6
AGAGTAC	47425	0.0	19.521421	10-11
>>END_MODULE